• Journal of Microbiology and Biotechnology
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• 제30권10호
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• pp.1516-1524
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• 2020

Climate change is expected to affect not only availability and quality of water, the valuable resource of human life on Earth, but also ultimately public health issue. A six-year monitoring (total 20 times) of Escherichia coli O157, Salmonella enterica, Legionella pneumophila, Shigella sonnei, Campylobacter jejuni, and Vibrio cholerae was conducted at five raw water sampling sites including two lakes, Hyundo region (Geum River) and two locations near Water Intake Plants of Han River (Guui region) and Nakdong River (Moolgeum region). A total 100 samples of 40 L water were tested. Most of the targeted bacteria were found in 77% of the samples and at least one of the target bacteria was detected (65%). Among all the detected bacteria, E. coli O157 were the most prevalent with a detection frequency of 22%, while S. sonnei was the least prevalent with a detection frequency of 2%. Nearly all the bacteria (except for S. sonnei) were present in samples from Lake Soyang, Lake Juam, and the Moolgeum region in Nakdong River, while C. jejuni was detected in those from the Guui region in Han River. During the six-year sampling period, individual targeted noxious bacteria in water samples exhibited seasonal patterns in their occurrence that were different from the indicator bacteria levels in the water samples. The fact that they were detected in the five Korea's representative water environments make it necessary to establish the chemical and biological analysis for noxious bacteria and sophisticated management systems in response to climate change.

• 농촌의학ㆍ지역보건
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• 제24권2호
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• pp.225-232
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• 1999

간흡충 및 요코가와흡충 만연 일 지역에서 주민 전체를 대변검사하여 간흡충 감염자를 찾아내고, 이들을 전원 디스토시드로 치료해 주고 2년 후에 유병률과 재감염율을 조사한 결과는 다음과 같다. 1. 간흡충 유병률은 투약 전 35.8%에서 투약 후 10.9%로 감소하였다(P<0.01). 같은 시기에 요코가와흡충도 17.0%에서 6.3%로 감소하였다. 2. 간흡충 감염자의 성비는 투약전후 차이가 없었고, 평균연령은 남녀 각각 투약 전 50, 40대에서 투약 후 남녀 공히 60대로 상승하였다. 연령별 분포에서 투약 전 10대에서 관찰되었던 감염자가 투약 후에는 관찰되지 않았고, 투약 전 40, 50, 60, 70대에서 감염자가 관찰되었으나 투약 후에는 50, 60대에서만 관찰되었다. 3. 간흡충 및 요코가와흡충 감염자를 치료하고 2년 후에 관찰한 재감염율은 각각 15.8%, 25.0%이었다. 4. 간흡충 감염자 중 재감염자와 신환감염자의 분율은 각각 50.0%, 50.0%이었다. 요코가와 흡충의 경우는 재감염자만 100.0%이었다. 이상의 결과에서, 섬진강 상류에 위치한 강변지역인 곡성군 오곡면 송정리에서 마을전 주민을 대상으로 실시한 간흡충 및 요코가와흡충의 관리는 비록 유병률이 투약 후에 감소하였지만 성공적으로 관리 또는 박멸되었다고 할 수 없다고 판단되며, 그 원인으로서 재 감염을 들 수 있으며 이 질환의 퇴치를 위해서는 특히 재감염의 관리가 우선적으로 필요하다고 생각된다.

• 농촌의학ㆍ지역보건
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• 제34권1호
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• pp.135-142
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• 2009

섬진강 유역 일개 군지역에서 1999년부터 2005년까지 간흡충 관리사업 결과 간흡충 감염률과 감염관련요인의 변화를 알아보기 위해 체계적 확률표본추출한 8개 마을주민 699명 중 416명에 대해 간흡충 충란검사를 위해 대변검사와 관련요인들의 특성을 파악하기 위해 설문조사를 하였으며, 1999년의 선행 연구와 비교하여 다음과 같은 성적을 얻었다 1. 간흡충 감염률은 1999년의 19.0%에서 2005년 11.3%로 유의하게 감소하였다 (P(Z)=0.000). 2. 간흡충 감염과 관련된 요인별 감염률은 남자와, 44세 이상자, 강변지역 거주자, 음주자 및 흡연자에서 유의하게 감소하였고, 그 결과로써 1999년에 성별, 연령별, 지역별, 생식습관, 음주여부와 같은 요인에 따라 감염률이 통계적으로 유의한 차이를 보였으나, 2005년에는 그러한 차이를 보이지 않았다. 3. 이전 간흡충 검사여부와 검사결과, 치료후 재섭취 여부에 관계없이 감염률이 유의하게 감소하였다. 4. 간흡충에 대한 인식 항목에서 간흡충증을 ‘잘안다’거나 ‘들어본적 있음’군에서 유의하게 감소하였고, 건강행위 실천여부에 에서는 안 한다고 하는 군에서 감염률이 유의하게 감소하였다. 결론적으로 간흡충 감염률은 5년 전에 비해 크게 감소하였다. 그 원인으로서는 지속적인 보건사업과 민물고기 생식률이 감소하고, 민물고기의 수나 종류가 감소하는 등 환경적인 변화가 작용한 것으로 보인다. 그러나 아직도 전국평균에 비하면 많이 높아서 간흡충 관리사업을 더욱 강화해야 할 것으로 생각된다. 또한 지속적인 간흡층관리사업의 결과, 감염 관련 요인으로 파악되었던 성별, 연령, 지역, 생식습관, 음주여부와 상관 없이 감염률이 감소되는 것으로 밝혀져 이와 같은 특성에 관계없이 전체 주민을 대상으로 간흡충 관리 사업이 확대되어야 할 것으로 사료된다.

• BMB Reports
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• 제51권10호
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• pp.532-537
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• 2018

Prostaglandin $E_2$ ($PGE_2$), a major product of cyclooxygenase-2 (COX-2), plays an important role in the carcinogenesis of many solid tumors, including colorectal cancer. Because $PGE_2$ functions by signaling through $PGE_2$ receptors (EPs), which regulate tumor cell growth, invasion, and migration, there has been a growing amount of interest in the therapeutic potential of targeting EPs. In the present study, we investigated the role of EP4 on the effectiveness of cordycepin in inhibiting the migration and invasion of HCT116 human colorectal carcinoma cells. Our data indicate that cordycepin suppressed lipopolysaccharide (LPS)-enhanced cell migration and invasion through the inactivation of matrix metalloproteinase (MMP)-9 as well as the down-regulation of COX-2 expression and $PGE_2$ production. These events were shown to be associated with the inactivation of EP4 and activation of AMP-activated protein kinase (AMPK). Moreover, the EP4 antagonist AH23848 prevented LPS-induced MMP-9 expression and cell invasion in HCT116 cells. However, the AMPK inhibitor, compound C, as well as AMPK knockdown via siRNA, attenuated the cordycepin-induced inhibition of EP4 expression. Cordycepin treatment also reduced the activation of CREB. These findings indicate that cordycepin suppresses the migration and invasion of HCT116 cells through modulating EP4 expression and the AMPK-CREB signaling pathway. Therefore, cordycepin has the potential to serve as a potent anti-cancer agent in therapeutic strategies against colorectal cancer metastasis.

• 대한핵의학회지
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• 제1권2호
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• pp.41-59
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• 1967

This study was aimed; firstly to observe various clinical symptomatology with reiation to the number of Ancylostoma duodenale larvae orally given to the human beings, secondly to evaluate the effects of some drugs like steroid hormones, antihistaminic3 and antitussives, and, thirdly to study the influences of some iron compounds in prevention and treatment of anemia of such origin. Ten healthy volunteers free from the previous history of hookworm infection were divided into 4 groups, to whom various numbers of actively moving filariform Ancylostoma duodenale larvae were orally given; 500 to 4 cases, 250 to 3 cases and 100 to 3 cases. Following were the results: 1. Clinical symptomatology 1. The most frequently encountered symptoms and signs were general malaise, cough and hoar seness. The tracheal itching and pain low back pain, arthralgia, sputum and salivation, acid belching, loss of appetite, abdominal pain and vomiting were also noted. 2. If the larger number of the larvae was given, the clinical symtomatology was more severe. 3. Prednisolone medication caused some improvement of such symptomatology, while the antihistaminics and antitussives like codeine or ephedrine were ineffective. 4. In volunteers whose nutritional conditions were rather poor appeared to show more severe symptomatology. 2. Effects of iron compounds 1. The oral administration of ferrous fumarate induced a slight increase of serum iron levels in the initial stage of the infection, then a decrease from $15{\sim}20$ days later and a recovery after 2 months. 2. The intravenous administration of saccharated ferric oxide induced a steady upkeep of the serum iron levels. 3. The hemoglobin contents also showed the upkeeps after either the oral or intravenous administration of the iron compounds. 4. The iron compounds, therefore, are considered to have the preventive as well as the therapeutic effects on hookworm anemia, which may strongly suggest that hookworm anemia is essentially the iron deficiency type. 3. Hematological changes 1. The severity of hookworm anemia generally correlated with the number of larvae given. 2. The moderate leucocytosis was observed in all cases regardless of the number of larvae given, which reached to a peak in $25{\sim}35$ days. 3. Eosinophilia was observed in all cases, but was more severe in cases given larger number of larvae, which was slightly less evident after the medication of prednisolone. 4. Red cell survival time The red cell survival time determined by $^{51}Cr$ was generally in the normal ranges except for the severe anemia patients.

• 농촌의학ㆍ지역보건
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• 제25권2호
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• pp.303-314
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• 2000

신안군 일부 섬지역에 거주하는 주민 224명에 대하여 장흡충 감염실태와 감염요인을 파악하기 위하여 설문조사와 대변에서의 장흡충 충란검사를 실시히여 다음과 같은 결과를 얻었다. 1. 조사대상자의 대변검사상 장흡충란양성률은 21.0%이었다. 2. 조사대상자의 장흡충란 양성률은 70세 이상에서 다른 연령군보다, 내륙지역보다 해안 지역에서, 농 수산에 종사하는자가 다른직업에 종사자보다 통계적요로 유의하게 높았다(P<0.05). 3. 연구대상자중 과거 음주자가 비음주자보다 장흡충 충란 양성률이 통계적으로 유의하게 높았고 현재 흡연자가 비흡연자에 비해 통계학적으로 유의하게 놓았다(P<0.05). 4. 장흡충 감염흡충은 장흡충의 중간 숙주인 숭어 생식자들에게서 통계학적으로 유의하게 높았다(P<0.01). 5. 연구대상자중 장흡충의 양성자 중에서 잦은 복통과 잦은 설사증상들이 통계학적으로 유의하게 높았다(P<0.05). 이상의 결과를 요약하면 전남 일부 섬지역의 장흡충 감염율은 여전히 높아 이에대한 진단 및 치료사업의 필요성이 충분하며, 해안지역 거주자, 고연령자, 음주, 흡연 그리고 생선회의 생식등이 관련이 있는 것으로 나타나 감염률을 낮추기 위해서는 이들 관련인자에 대한 우선적 접근이 필요하며 계속적인 관심과 관리가 이루어져야 할 것으로 생각한다.

• 농촌의학ㆍ지역보건
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• 제25권2호
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• pp.441-448
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• 2000

This study was performed to investigate the prevalence and its related factors on the Clonorchis sinensis(C.S.) of inhabitants at Goksung-gun, Chollanam-do. After the population was stratified by gender, age, resident area, 651 residents living in eight villages were sampled by cluster sampling method. We interviewed the subjects to survey the various characteristics using the questionnaire, and examined stools to confirm the infection of C.S. from November to December, 1998. The prevalence of C.S. was 19.0%. The factors related with the infection of C.S. were male(adjusted odds ratio, of female=1.71, 95% confidence interval=1.07-2.72), 45-64 year old group(aOR of under 45 year old group=2.16. 95% CI=1.21-3.85), above 65 year old group(aOR of under 45 year old group=2.34, 95% CI=1.31-4.15), basin villagers(aOR of inland villagers=2.34, 95% CI=1.31-4.15), current drinker(aOR of nondrinker=1.83, 95% CI=1.12-2.98), those who took raw fish(aOR of persons who didn't it or take cooked fish=2.09, 95% CI=1.21-3.88) and persons who know the infection status(aQR of the persons who didn't know it=0.57, 95% CI=0.37-0.89). In conclusion, these results suggest that several life styles such as ingestion of raw fish, drinking habit. So we think that it is necessary to set up the efficient management programs for the treatment and prevention of C.S. infection.

• Journal of Ginseng Research
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• 제40권2호
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• pp.160-168
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• 2016

Background: Ginseng (Panax ginseng Meyer) is a well-characterized medicinal herb listed in the classic oriental herbal dictionary as "Shin-nong-bon-cho-kyung." Ginseng has diverse pharmacologic and therapeutic properties. Black ginseng (BG, Ginseng Radix nigra) is produced by repeatedly steaming fresh ginseng nine times. Studies of BG have shown that prolonged heat treatment enhances the antioxidant activity with increased radical scavenging activity. Several recent studies have showed the effects of BG on increased lipid profiles in mice. In this study report the effects of water and ethanol extracts of BG on hypercholesterolemia in rats. To our knowledge, this is the first time such an effect has been reported. Methods: Experiments were conducted on male Sprague Dawley rats fed with a high-cholesterol diet supplemented with the water and ethanol extracts of BG (200 mg/kg). Their blood cholesterol levels, serum white blood cell levels, and cholesterol-metabolizing marker genes messenger RNA (mRNA) expression were determined. Liver and adipose tissues were histologically analyzed. Results: We found that BG extracts efficiently reduced the total serum cholesterol levels, low-density lipoprotein (LDL) levels with increased food efficiency ratio and increased number of neutrophil cells. It also attenuated the key genes responsible for lipogenesis, that is, acetyl-coenzyme A (CoA) acetyltransferase 2, 3-hydroxy-3-methyl-glutaryl-CoA reductase, and sterol regulatory element-binding protein 2, at the mRNA level inside liver cells. Furthermore, the BG extract also reduced the accumulation of fat in adipose tissues, and inhibited the neutral fat content in liver cells stained with hematoxylin and eosin and oil red O. Conclusion: Administration of BG extracts to Sprague Dawley rats fed with high-cholesterol diet ameliorated hypercholesterolemia, which was mediated via modulation of cholesterol-metabolizing marker genes. This data throw a light on BG's cardioprotective effects.

• 농촌의학ㆍ지역보건
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• 제22권2호
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• pp.239-252
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• 1997

A questionnaire survey including stool examination for the parasite ova, skin test, and enzyme-linked immunosorbent assay (ELISA) of the liver fluke to find the factors related with the prevalence of the parasite infestation was done toward 118 residents living in three villages of Song-jung, Yi-jung, and Chim-gok of Okok-myun, Goksung County, South Cholla Province. The results are as follows : 1. The prevalence of Clonorchis sinensis ova by the stool examination was 46.6%. The sensitivity and specificity of skin test to egg-positive cases were 63.5% and 68.9%. The sensitivity and specificity of ELISA to egg-positive clonorchiasis were 100% and 34.9%. 2. The prevalence of Clonchiasis was higher in male than in female. 45~64 year old group was the highest among age groups, and the longer the duration of living in the present residence was, the higher the prevalence was shown statistically significantly (p<0.01). 3. The present drinker had higher prevalence of Clonorchiasis significantly than the past drinker or non-drinker, and the more the frequency of drinking the higher the prevalence was shown significantly (p<0.05). 4. Those who took raw fish had significantly higher than persons who didn't take it or take cooked fish(p<0.05) and prevalence showed the tendency to be increased as the frequency to take raw fishes increased significantly(p<0.05). 5. Those who had taken a raw sweet fish(Plecoglossus altivelis) or snake-headed fish(Ophicephalus argus) showed significantly higher prevalence of infection with liver fluke than persons who didn't take the raw fish. 6. While 56.4% of persons infected with Clonorchiasis answered that they were healthy, only 13.0% of the subjects were careful for their health, and 41.8% experienced tests to diagnose liver flukes before they were examined by this survey. 7. While 56.4% of infected persons thought the prevalence was high in their community, 54.0% were afraid to be infected, 47.1% thought the infection might destroy their health, 25.5% took treatment drugs, and 18.2% took raw fish continuously after treatment. The above results confirmed that Goksung area was highly prevalent in Clonorchiasis, so it was necessary to push effective preventive program against liver fluke infection together with the program to diagnose and treat existing infected persons.

• 대한의생명과학회지
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• 제11권3호
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• pp.259-266
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• 2005

Mast cells and goblet cells have been known to protect the host against parasites. In this study, we examined the response of the mast cells and goblet cells over a period of 6 weeks in the duodenum, jejunum and ileum of C3H/HeN and C57BL/6 mice infected with Echinostoma hortense (E. hortense). In addition, we investigated whether the worm recovery rate of uninfected mice (the control group) or E. hortense-infected mice (the experimental group) was associated with the number of mast cells and goblet cells. The worm recovery rate was higher in the C3H/HeN mice than in the C57BL/6 mice. The number of goblet cells significantly increased in the experimental group of the C3H/HeN and C57BL/6 mice compared with the control group of both strains (P<0.005). Worm recovery peaked 3 weeks after the infection of the C57BL/6 mice and at 2 weeks after the infection of the C3H/HeN mice, and it was higher in the duodenum than in the jejunum and ileum. However, the infected site in the intestine had no relation with worm expulsion. In the C3H/HeN and C57BL/6 mice, the number of goblet cells in the experimental group was significantly higher than that in the control group (P<0.005). The number reached a peak 2 weeks after the infection and it even increased in duodenum, jejunum and ileum. The increased number of goblet cells was retained 6 weeks after infection. The number of goblet cells was higher in the C3H/HeN mice than in the C57BL/6 mice (P<0.01). These results indicate that goblet cells are related with the worm expulsion. Furthermore, immunohistostaining of the antral intestinal walls for lectin showed the significant increase of the number of goblet cells in the experimental group (P<0.001). The high infection rate in the duodenum was found during the early infection. An increased infection rate in the jejunum and ileum was found 3 weeks after infection and the infection rate was higher in the C3H/HeN mice than in the C57BL/6 mice. Taken together, the present study indicates that goblet cells, rather than mast cells, may play critical roles in parasite expulsion.