• 한국가축위생학회:학술대회논문집
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• 한국가축위생학회 1996년도 제19차 학술대회 및 정기총회
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• pp.69.2-70
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• 1996

• 한국어업기술학회:학술대회논문집
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• 한국어업기술학회 1997년도 수산관련공동학술대회발표요지집
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• pp.338-339
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• 1997

• 한국어류학회지
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• 제28권3호
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• pp.141-146
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• 2016

본 연구에서는 넙치 유래 VHSV (genotype IVa)에 의한 무지개송어의 영향을 조사하기 위하여 다양한 조건에서 감염 실험을 실시하였다. 3개의 다른 로트의 담수 무지개송어 치어에 VHSV ($10^{6.3-7.3}TCID_{50}$/fish)를 복강 주사한 결과, 15% 이하의 누적 폐사율이 관찰되었다. 무지개송어 (in vivo)를 사용하여 VHSV를 계대 배양한 후 감염 실험을 실시한 결과, VHSV를 5회 계대 배양하여도 독력의 변화는 관찰되지 않았다. 해수에 사육 중인 송어 (성어)에 넙치 유래 VHSV ($10^{5.3}TCID_{50}$/fish와 $10^{6.3}TCID_{50}$/fish)를 복강 주사한 결과, 폐사어는 관찰되지 않았고, 어체 내에서도 VHSV는 검출되지 않았다. 이상의 결과, 해수 송어 (성어)에서는 넙치 유래 VHSV의 병원성이 확인되지 않았으나 담수 무지개송어 치어에서는 낮은 독력을 나타내었다.

• 한국수산과학회지
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• 제38권5호
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• pp.291-297
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• 2005

This study evaluated the quality of commercial extruded pellet (EP) diet of five companies (A, B, C, D and E) for olive flounder Paralichtys olivaceus by biochemical analyses, physical properties and growth performance. The proximate analyses of five EP diets showed $3.2-10.0\%$ of moisture, $49.3-55.5\%$ of crude protein, $4.6-14.7\%$ of crude lipid, $7.0-13.8\%$ of crude ash, $0.7-10.5\%$ of crude fiber, $10.0-27.3\%$ of itrogen free extract (NFE), 304.3-395.4kcal/100g of digestible energy (DE) and 6.1-7.1 of calorie/protein ratio (C/P). Peroxide value (POV) was highest in diet D (47.4 meq/kg) as compared to other diets which in the range of 4.0-11.7 meq/kg. Total amino acid contents were ranged from 46.54 to $55.46\%$ with the highest content in diet B and the lowest content in diet C. Essential amino acid of diet C was lowest $(7.43\%)$ as compared to other diets which in the range of $19.43-20.30\%$. Saturated fatty acid was higher in diet A $(37.65\%)$ followed by diet B $(36.32\%)$, diet E$(34.39\%)$, diet C$(30.95\%)$ and diet D$(30.10\%)$. EPA+DHA were highest in diet E$(30.78\%)$ and lowest in diet C$(15.48\%)$. The floating rate after 6 hours on the sea water was highest in diet C$(100\%)$ followed by diet B$(40\%)$ and A$(10\%)$. However, diets D and E were completely settled down after 1 and 2 hours, respectively. The range of relative expansion rate was $27.2-49.3\%$ for all diets and all reached the peak at 2-3 hours. The water absorption rate of diets C and D was lowest, and diet E was highest at 1 hour after deposition of sea water. Growth rate was higher in diet B$(22.3\%)$ and E$(21.3\%)$. Feed efficiency was higher in diet A$(109.7\%)$ and E$(105.3\%)$ and was significantly lowest in diet D$(80.7\%)$. The protein efficiency ratio was highest in diet E (2.72) and lowest in diet D (1.76). These results suggest that there is a necessity for improvement of nutrients balance and feed physical properties to fulfill the nutrient requirements and digestive characteristics of fishes in commercial EP diets.

• 수산해양교육연구
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• 제28권6호
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• pp.1822-1827
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• 2016

Consumption of Paralichthys olivaceus in raw fish have been reported as the cause of outbreaks of food-born illness, and Kudoa septempunctata in muscle of Paralichtys olivaceus was suggested with the causative agent. For this reason, distinguish of vital and dead spore is important to study survivability of Kudoa septempunctata in human intestinal condition and in vitro inactivation of Kudoa septempunctata. In this reports, we suggest NR & MB (Neutral red and Methylene blue) staining method that is easier and simpler than the previously described HO & PI (Hoechst33342 and Propidium iodide) method according to a experimental condition.

• 생명과학회지
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• 제11권4호
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• pp.371-378
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• 2001

The effects of the RM 60 series on DNA replication systems were examined by using simian virus 40 (SV40) DNA replication system in vitro. The RM 60 series inhibited the DNA replication in the initiation step rather than in the elongation step. Polymerase$\alpha$-primase activity and toposiomerase I activity study were performed subsequently, the RM 60 seies increased the polymerase $\alpha$-primase activity in a low dose, but decreased the activity in a higher dose. The topoisomerase I activity was inhibited by the RM 60 series. This result suggests that the RM 60 series might inhibit some molecules which are required to establish replication forks during the initiation step of the DNA replication.

• 한국어병학회지
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• 제17권3호
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• pp.179-189
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• 2004

Morphological structure and sporogenesis of myxosporean parasite, Parvicapsula anisocaudata from cultured olive flounder, Paralichthys olivaceus were examined by light and transmission electron microscopies. Numerous round early stages, spindle-shaped disporic or monosporic pseudoplasmodia, asymmetrical thin-walled mature spores were found inthe lumen of renal tubule and urinary bladder. Long pseudopodia or short projections from cytoplasm of pseudoplasmodia make the parasite attach firmly to host tissues. Spore development was mono- or disporous with no pansporoblast formation. Sporoplasmic cell was partially surrounded by two capsulogenic cells, and capsulogenic cells were enveloped by two flattened valvogenic cells. Capsulogenesis and valvogenesis followed general patterns seen in that of other myxosporean.

• 한국수산과학회지
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• 제36권6호
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• pp.654-660
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• 2003

To evaluate the biological diversity of fish pathogenic streptococci, 35 strains isolated from diseased olive flounder (Paralichtys olivaceus), were analyzed using a random amplified polymorphic DNA (RAPD) technique with the oligonucleotide commercial primer 6 (Amersham Biosciences). Api 20 Strep test, drug resistance and artificial infection were carried out for further characterization of the isolates. RAPD fingerprints showed similar pattern in 25 strains (about $71.4\%$ of 35 isolates) and these strains were designed as RA group 1. Similarities greater than $44\%$ were obtained when the Dice coefficient was applied among the isolates of RA 1. On the other hand, the reference Streptococcus iniae showed a similar RAPD profile to the isolates with similarity levels of $40-93.3\%.$ Rh I was suggested to be the dominant group isolated from olive flounder suffering from streptococcosis. However, the isolates of Rh 1 group were not classified into the same species by the Api 20 Strep identification system. There was no peculiarity in drug resistance patterns of Rh I group isolates against 7 antibacterial agents. However, only 3 of 25 isolates $(0.12\%)$ showed oxytetracycline (OTC) resistance and OTC might be a useful chemotherapeutic agent in controlling the streptococcosis by strains of RA I group in olive flounder. Fish injected intraperitoneally with $10^5$ CFU of an isolate of Rh I and RA III group showed $60\%\;and\;50\%$ accumulative mortality for 20 days, respectively ($20\%$ in control or Rh II). However luther comparative studies about differences in virulence between isolates are needed.

• 수산해양교육연구
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• 제26권3호
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• pp.543-552
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• 2014

Papain family중 하나인 cysteine protease는 근골격계 질환 치료를 위한target molecule로 인식 되어왔으며 Cathepsin B 는 단백질 분해의 초기과정에 관여하는 cysteine proteases 중 하나이다. 본 연구는 넙치의 cathepsin B 유전자의 발현 양상과 넙치 cathepsin B(PoCtB)의 클로닝, 발현 및 효소특성을 분석하였다. cDNA Library Screening을 이용하여 넙치의 cDNA를 클로닝하였다. 넙치의 동정된 cathepsin B 유전자는 993bp의 open reading frame과 330개의 아미노산으로 이루어져있다. Cathepsin B의 propeptide region 내에 GNFD motif와 occluding loop 가 존재함으로써 이것이 명백하게 cathepsin B group이라는 것을 보여주며, 계통 유전학적 분석 결과 다른 종의 cathepsin B에 비해 초창기에 분화되어 나온 것으로 사료된다. mature enzyme인 maPoCtB은 fusion protein인 glutathione S-transferase를 포함하는 pGEX-4T-1 vector에 삽입하여 E.coli 균주인 $DH5{\alpha}$ 내에 발현시켰다. 재조합 단백질인 PoCtB을 과발현 시킨 결과 53kDa의 분자량을 가진다. 넙치 cathepsin B 활성은 Z-Arg-Arg-AMC와 같은 fluorogenic 펩타이드 기질을 이용하여 측정되었고 적정 pH는 pH.7.5 이다.

• 한국양식학회지
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• 제15권1호
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• pp.15-21
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• 2002

관행의 일일공급체제를 격일공급체제로 전환할 경우 넙치의 사료 섭취량과 증체량이 어떤 변화를 보이는지 조사하고 습사료의 공급에 따른 질소와 인의 배설량을 측정 하기 위하여 펑균 체중 90 g의 넙치를 이용하여 양어장에서 8주간 사육실험을 수행하였다. 일일공급 (ED)구의 건물 섭취량은 펑균 107.7g으로 격일공급 (EO)구의 평균 섭취량 89.8 g에 비해 유의적으로 높게 나타났다. 반면, 사료전환효율은 각각 1.51 (ED) 및 1.47 (EO)로 유의적 차이가 나타나지 않았다. 마리당 증체량은 섭취량이 높았던 ED구에서 마리당 71.6 g으로 EO구 (61.1 g)보다 높게 나타났으나 유의적 차이는 발견되지 않았다 (P>0.05). 질소 증가량은 질소 섭취량이 유의적으로 높았던 ED구가 2.13g으로 EO구 (1.76 g)보다 유의적으로 높게 나타났다. 그러나 증체단위당 질소 배설량은 두 처리구 공히 약 113 g으로 동일하였다. 인 섭취량은 유의적인 차이를 보였으나 어체내 인 증가량은 공히 0.5 g으로 동일하였다. 증체 단위당 인 배설량은 ED구가 27.6 g으로 EO구 (26.0 g)에 비해 약간 높았으나 유의적 차이는 없었다. 에너지 섭취량은 ED구가 2523 kJ로 EO구 (2116 kJ)에 비해 유의적으로 높았으며 그 결과 체내 에너지 증가량도 ED구가 유의적으로 높았다. 그러나, 에너지 축적효율은 각각 20.6% (ED) 및 18.9% (EO)로 유의적인 차이가 발견되지 않았다. 이상의 결과는 관행의 일일공급체제를 격일공급체제로 전환할 수 있는 가능성을 제시하였다.