• Title/Summary/Keyword: Paraffin-embedded tissues (PET)

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Detection of Mycobacterium tuberculosis by PCR from Trace Clinical Specimens and Paraffin-embedded Tissues (임상가검물과 파라핀 포매 조직에서 PCR법을 이용한 결핵균의 검출)

  • 김은중;최우순;황석연
    • Biomedical Science Letters
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    • v.6 no.1
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    • pp.55-63
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    • 2000

  • This study has been carried out to investigate the sensitivity of polymerase chain reaction (PCR) method over conventional acid-fast bacilli (AFB) staining and/culture methods for the detection of Mycobacterium tuberculosis from trace body fluid and paraffin-embedded tissues (PET) specimens. A total of 65 cases were employed for the AFB staining and culture test, and a total of 50 cases were subjected to PCR and histopathological analysis. Among the specimen showing negative reaction to AFB staining, 12.1% were positive to PCR and 3.7% of the specimen representing negative result to culture test showed positive reaction to PCR. In addition, 20.0% of the specimen with AFB negative showed positive reaction to PCR. From these results, it could be concluded that PCR method overwhelms AFB staining and culture tests in sensitivity and specificity to M tuberculosis detection.

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