• Title/Summary/Keyword: Paraconiothyrium brasiliense

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First Record of Endophytic Paraconiothyrium brasiliense Isolated from Chinese Maple Leaves in Korea

  • Paul, Narayan Chandra;Lee, Hyang Burm
    • The Korean Journal of Mycology
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    • v.42 no.4
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    • pp.349-352
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    • 2014
  • The fungal endophyte EML-CM25 was isolated in Korea from surface sterilized Chinese maple leaf tissues. Internal transcribed spacer sequence analysis indicated that the isolate showed 99% sequence similarity with Paraconiothyrium brasiliense, a finding that was confirmed by morphological analyses. The fungal colonies did not express aerial hyphae. Conidiomata formation was observed in the fungus cultured on potato dextrose agar at $25^{\circ}C$ for 7 days. Visible scattered black dots consisting of pycnidia were present throughout the colony. This is the first record of P. brasiliense in Korea.

Identification and Characterization of Paraconiothyrium brasiliense from Garden Plant Pachysandra terminalis (가든식물 수호초(Pachysandra terminalis)로부터 Paraconiothyrium brasiliense의 분리 및 동정)

  • Choi, Min Ah;Park, Seung Jun;Ahn, Geum Ran;Kim, Seong Hwan
    • The Korean Journal of Mycology
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    • v.42 no.4
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    • pp.262-268
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    • 2014
  • A fungal isolate DUCC5000 from a garden plant Pachysandra terminalis was identified as Paraconiothyrium brasiliense based on the results of morphological and molecular studies. The fungus formed brown to black conidiomata of (0.2-0.7)-2(-3.5) mm singly or as a group on PDA. Conidia measured $2-5{\times}1.8-3{\mu}m$ in size, hyaline, ellipsoid to short-cylindrical, and rounded at both ends. The internal transcribed spacer (ITS) DNA of the isolate shared 100% nucleotide sequence homology with those of known P. brasiliense isolates. Phylogenetic tree inferred from the ITS sequence analysis showed that the DUCC5000 isolate formed a clade with known isolates of P. brasiliense. The fungal mycelia grew better on oatmeal agar than on MEA and PDA. On PDA media under various pH conditions, fungal mycelial growth was observed at pH 9. Colony morphology of the fungus tended to alter depending on the kinds of nutrient media and pH condition. On chromagenic media, the fungus demonstrated its ability to produce extracellular enzymes including amyalse, avicelase, ${\beta}$-glucosidase, protease, and xylanase. However, in pathogenicity testing, no disease symptoms were observed on the leaves of P. terminalis. This strain is the first report on P. terminalis in Korea.

Diversity of Foliar Endophytic Fungi Isolated from Lindera obtusiloba in Korea (생강나무(Lindera obtusiloba)의 잎에서 분리한 내생균의 다양성)

  • Kim, Chang-Kyun;Eo, Ju-Kyeong;Eom, Ahn-Heum
    • The Korean Journal of Mycology
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    • v.40 no.3
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    • pp.136-140
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    • 2012
  • Leaves of Lindera obtusiloba were collected from four sites in Gangwon Province, Korea. Endophytic fungi were isolated from the leaves and identified using ITS sequences of rDNA. Total twelve species belonging of endophytic fungi were identified; Alternaria alternata, Annulohypoxylon annulatum, Creosphaeria sassafras, Diaporthe eres, Discosia sp., Epicoccum nigrum, Glomerella acutata, Glomerella cingulata, Paraconiothyrium brasiliense, Pestalotiopsis neglecta, Phomopsis amygdali, Xylaria sp. The endophytic fungus, Phomopsis amygdali, was the most dominant species isolated from L. obtusiloba in this study and the fungal diversities varied in the different sites.