• Proceedings of the PSK Conference
• /
• 2003.04a
• /
• pp.318-318
• /
• 2003

The current study was performed to observe the effects of Panax ginseng (PG) and P. quinquefolium (PQ) on hemodynamics such as blood flow rate (BF), blood flow velocity (BV), heart rate (HR), systolic blood pressure (SBP), and diastolic blood pressure (DBP) and body temperature (BT) in healthy young men. This is a randomized, single-blind study observed during 6 hrs after orally single administration of PG and PQ groups. (omitted)

• Journal of Ginseng Research
• /
• v.12 no.2
• /
• pp.145-152
• /
• 1988

Correlations between various morphological characteristics of Panax quinquefolium plants grown in Lytton, British Columbia, Canada were assessed for 1-through 4-year old plants. Root dry weight, the dependent variable, was found to be strongly related to leaf dry weight, leaf length and root length for 1-and 2-year old plants during the middle of the growing season. For 1- and 2-year old plants at the end of the growing season, root dry weight was found to be related to leaf dry weight, leaf length and stem dry weight. For 3 and 4-year old plants, root dry weight was found to be related to leaf dry weight, leaf length and stem dry weight. For 3- and 4-year old plants, root dry weight was found to be related to leaf dry weight. For practical considerations, this latter relationship provides a simple method for selecting superior plants from which seed can be harvested.

• Journal of Ginseng Research
• /
• v.19 no.2
• /
• pp.171-174
• /
• 1995

Freshly harvested American ginseng (Panax quinquefolium L.) seeds were stratified at two locations over each of three years. Seed development and temperature in the stratification boxes were investigated until the seed was removed 12 months later and direct-seeded in the field. During stratification and seeding (14 months) three embryo stages were identified. In Stage I of 250 days (Sept. to mid-May) embryo length increased from about 0.5 to 1.0 mm: in Stage II of 100 days (mid-May to late Aug. when seeded) length increased to 2.0 mm and in Stage III (late Aug. to late Nov.) length increased to 5.3 mm. Excerpt split width could also be placed in three stages. Changes in embryo length correlated with embryo endosperm length ratio. Insertion compression tests showed that the excerpt softened rapidly in late Stage II and throughout Stage III. The stratification box temperatures at all depths (10, 25 and 50 cm) never exceeded -2$^{\circ}C$ even when the air temperatures dropped to -13$^{\circ}C$ and were, therefore not damaging to the seeds.

• Korean Journal of Pharmacognosy
• /
• v.45 no.2
• /
• pp.107-112
• /
• 2014

The purpose of this study is to develop a new preparation process of American ginseng (Panax quinquefolium) extract featuring high concentration of ginsenoside $Rg_3$, $Rg_5$, and $Rk_1$, Red ginseng special components. Chemical transformation from ginseng saponin glycosides to prosapogenin was analyzed by the HPLC. Extracts of American ginseng were processed under several treatment conditions of microwave and vinegar (about 14% acidity). The results showed that the quantity of ginsenoside $Rg_3$ increased by over 0.9% at the 20 minutes of the pH 2~4 vinegar and microwave American ginseng ethanol extract compared with other process times. The result of MAG-20 indicates that the American ginseng microwave and vinegar-processed American ginseng extracts (about 14% acidity) treated for 20 minutes produced the highest amount of ginsenoside $Rg_3$ (0.969%), $Rg_5$ (1.071%), and $Rk_1$ (0.247%). Besides, MAG-15 indicates that the microwave - and vinegar-processed American ginseng extracts (about 14% acidity) treated for 15 minutes produced the highest amount of ginsenoside $Rg_3$ (0.772%), $Rg_5$ (1.330%), and $Rk_1$ (0.386%). This indicates that American ginseng treated with microwave and vinegar had the quantity of the ginsenoside $Rg_3$ over 32 times the amount of the ginsenoside $Rg_3$ (which was not found in raw and American ginsengs) in the average commercial Red ginseng.

• Journal of Ginseng Research
• /
• v.44 no.1
• /
• pp.44-49
• /
• 2020

Background: Salting-out extraction (SOE) had been developed as a special branch of aqueous two-phase system recently. So far as we know, few reports involved in extracting ginsenosides with SOE because of the lower recovery caused by the unique solubility and surface activity of ginsenosides. A new SOE method for rapid pretreatment of ginsenosides from the enzymatic hydrolysates of Panax quinquefolium was established in this article. Methods: The SOE system comprising ethanol and sodium carbonate was selected to extract ginsenosides from the enzymatic hydrolysates of Panax quinquefolium, and HPLC was applied to analyze the ginsenosides. Results: The optimized extraction conditions were as follows: the aqueous two-phase extraction system comprising ethanol, sodium carbonate, ethanol concentration of 41.51%, and the mass percent of sodium carbonate of 7.9% in the extraction system under the experimental condition. Extraction time had minor influence on extraction efficiency of ginsenosides. The results also showed that the extraction efficiencies of three ginsenosides were all more than 90.0% only in a single step. Conclusion: The proposed method had been successfully applied to determine ginsenosides in enzymatic hydrolysate and demonstrated as a powerful technique for separating and purifying ginsenosides in complex samples.

• Journal of Ginseng Research
• /
• v.43 no.1
• /
• pp.27-37
• /
• 2019

Background: The structural conversions in ginsenosides induced by steaming or heating or acidic condition could improve red ginseng bioactivities significantly. In this paper, the chemical transformations of red American ginseng from fresh Panax quinquefolium L. under steaming were investigated, and the possible mechanisms were discussed. Methods: A method with reversed-phase high-performance liquid chromatography coupled with linear ion trap mass spectrometry ($HPLC-MS^n$)-equipped electrospray ionization ion source was developed for structural analysis and quantitation of ginsenosides in dried and red American ginseng. Results: In total, 59 ginsenosides of protopanaxadiol, protopanaxatriol, oleanane, and ocotillol types were identified in American ginseng before and after steaming process by matching the molecular weight and/or comparing $MS^n$ fragmentation with that of standards and/or known published compounds, and some of them were determined to be disappeared or newly generated under different steaming time and temperature. The specific fragments of each aglycone-type ginsenosides were determined as well as aglycone hydrated and dehydrated ones. The mechanisms were deduced as hydrolysis, hydration, dehydration, and isomerization of neutral and acidic ginsenosides. Furthermore, the relative peak areas of detected compounds were calculated based on peak areas ratio. Conclusion: The multicomponent assessment of American ginseng was conducted by $HPLC-MS^n$. The result is expected to provide possibility for holistic evaluation of the processing procedures of red American ginseng and a scientific basis for the usage of American ginseng in prescription.

• Journal of Ginseng Research
• /
• v.20 no.1
• /
• pp.42-48
• /
• 1996

This study was carried out to determine the differences of essential oil components among Korean, Chinese and Japanese red ginseng, and Korean white ginseng (Panax ginseng C.A Mayer) , American and Canadian ginseng (P. Quinquefolium), and sanchi ginseng (P notoginseng). The steam distilled oils of these ginsengs were analyzed by GC and GC-MS, and 22 sesquiterpenes, 8 sesquiterpene alcohols, 8 monoterpenes, 5 aldehydes, 4 esters, 3 acids, 2 alcohols and 5 miscellaneous components were identified. The major oil components of Korean, Chinese and Japanese red ginseng were $\beta$-panasinsene, $\beta$-caryophyllene, $\alpha$-panasinsene, $\alpha$-neoclovene, selina-4,11-diane, bicyclo-ger-macrene and spathulenol. The contents of $\beta$-panasinsene, $\alpha$-neoclovene, $\alpha$-basabolene and spathulenol were higher in Korean red ginseng than Chinese and Japanese red ginseng. The contents of $\alpha$-cubebene, selina-4,11-diene and ledol were higher in Chinese red ginseng than Korean and Japanese red ginseng, but those of selina-4,11-diene and spathulenol were lower in Japanese red ginseng than Korean or Chinese red ginseng. On the other hand, the GC patterns of the oils from American, Canadian and sanchi ginseng were different from that of Korean white ginseng.

• Journal of Ginseng Research
• /
• v.9 no.2
• /
• pp.204-212
• /
• 1985

Lipid and fatty acid compositions of the bound lipids in Panax quinquefolium (Korea, Japan and China), Panax quinquefolium (America, Canada) and Panax notoginseng (China) were studied by means of silicic acid column chromatography, thin-layer chromatography and gas-liquid chromatography. The could lipid contents in various ginsengs were 0.29 to 0.48%, in which neutral lipid fractions were 63.6 to 67.3%, glycolipid fractions 21.9 to 25.7% and phospholipid fractions 7.7 to 12.4%. The content compositions of neutral lipid fractions were lower and those of glycolipid and phospholipid fractions were higher in the bound lipids than in the free lipids from the various ginseng. The major components were fatty acids, diglycerides and free sterols in neutral lipid fractions, monogalactosyl diglyceride, sterol glucoside and esterified steryl g1ycoside in glycolipid fractions and phosphatidyl glycerol, phosphatidyl ethanolamine and phosphatidic acid in phosphoipid fractions. Seventeen fatty acids were analyzed in the four bound lipid fractions from the various ginsengs and main fatty acids were linoleic acid, palmitic acid and oleic acid. Total saturated fatty acid and palmitic acid contents were higher and total unsaturated fatty acid and linoleic acid contents lower in the total bound lipids than in the total free lipids from the various ginsengs.

• Journal of Ginseng Research
• /
• v.48 no.4
• /
• pp.354-365
• /
• 2024

Panax species include Panax ginseng Meyer, Panax quinquefolium L., Panax notoginseng, Panax japonicum, Panax trifolium, and Panax pseudoginseng, which contain bioactive components (BCs) such as ginsenosides and polysaccharides. Recently, growing evidence has revealed the pharmacological effects of Panax species and their BCs on allergic airway diseases (AADs), including allergic asthma (AA) and allergic rhinitis (AR). AADs are characterized by damaged epithelium, sustained acquired immune responses with enforced Th2 responses, allergenspecific IgE production, and enhanced production of histamine and leukotrienes by activated mast cells and basophils. In this review, we summarize how Panax species and their BCs modulate acquired immune responses involving interactions between dendritic cells and T cells, reduce the pro-inflammatory responses of epithelial cells, and reduce allergenic responses from basophils and mast cells in vitro. In addition, we highlight the current understanding of the alleviative effects of Panax species and their BCs against AA and AR in vivo. Moreover, we discuss the unmet needs of research and considerations for the treatment of patients to provide basic scientific knowledge for the treatment of AADs using Panax species and their BCs.

• Journal of Ginseng Research
• /
• v.10 no.1
• /
• pp.76-79
• /
• 1986

Variation in the inflorescence of Oriental ginseng (Panax ginseng C.A. Meyer) have been placed in 6 groups; only 2 of these groups, a complete simple hemispherical terminal umbel, and a simple umbel with several branched pedicels below it on the peduncle were found in cultivated American ginseng. Apical peduncle reflexing and associated peduncles shortening were observed in a few plants.