• Journal of Ginseng Research
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• 제41권4호
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• pp.463-468
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• 2017

Background: Both Panax ginseng Meyer and Panax quinquefolius are obligate shade-loving plants whose natural habitats are broadleaved forests of Eastern Asia and North America. Panax species are easily damaged by photoinhibition when they are exposed to high temperatures or insufficient shade. In this study, a cytohistological study of the leaf structures of two of the most well-known Panax species was performed to better understand the physiological processes that limit photosynthesis. Methods: Leaves of ginseng plants grown in soil and hydroponic culture were sectioned for analysis. Leaf structures of both Panax species were observed using a light microscope, scanning electron microscope, and transmission electron microscope. Results: The mesostructure of both P. ginseng and P. quinquefolius frequently had one layer of non-cylindrical palisade cells and three or four layers of spongy parenchymal cells. P. quinquefolius contained a similar number of stomata in the abaxial leaf surface but more tightly appressed enlarged grana stacks than P. ginseng contained. The adaxial surface of the epidermis in P. quinquefolius showed cuticle ridges with a pattern similar to that of P. ginseng. Conclusion: The anatomical leaf structure of both P. ginseng and P. quinquefolius shows that they are typical shade-loving sciophytes. Slight differences in chloroplast structure suggests that the two different species can be authenticated using transmission electron microscopy images, and light-resistant cultivar breeding can be performed via controlling photosynthesis efficiency.

• 대한한의학방제학회지
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• 제32권1호
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• pp.83-90
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• 2024

Objectives : As a substitute for high-price ginseng, this study attempted to examine a possibility of the ferment extract of Panax ginseng sprout whether leaves and roots can be used together as a cosmetic ingredient with anti-oxidative and wrinkle-care effects. Methods : In terms of a test method, antioxidant activities were confirmed through total polyphenol contents, total flavonoid contents, DPPH radical scavenging activity and ABTS radical scavenging activity using the Panax ginseng sprout. In addition, to assess wrinkle-care effectiveness, the cytotoxicity of the extract was analyzed through MTT assay, and inhibition of collagenase activity in the cells was tested using the Panax ginseng sprout fermented by Saccharomyces cerevisiae. Resuits : The content of polyphenols and flavonoids in natural plants was highest in Panax Ginseng Sprout Extract at 100℃, which also demonstrated high DPPH, ABTS radical scavenging activity. MTT assay demonstrated that the Panax Ginseng Sprout Ferment Extract did not have a cytotoxic effect in CCD-986SK cell. Also, Panax Ginseng Sprout Ferment Extract was found to inhibit MMP-1 expression by 51.85±6.09% at a concentration of 10%. Conclusions : Therefore, this study has confirmed a possibility of Panax ginseng sprout ferment extract as a cosmetic ingredient with MMP-1-inhibitory effects.

• Journal of Ginseng Research
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• 제38권4호
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• pp.289-292
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• 2014

Ginseng leaf/stem extract produced by subcritical water extraction at high temperature ($190^{\circ}C$) posses higher cytotoxic activity against human cancer cell lines than ethanol extract. Subcritical water extraction can be a great candidate for extraction of functional substance from ginseng leaves/stems.

• Journal of Ginseng Research
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• 제46권1호
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• pp.23-32
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• 2022

Panax polysaccharides are biopolymers that are isolated and purified from the roots, stems, leaves, flowers, and fruits of Panax L. plants, which have attracted considerable attention because of their immunomodulatory activities. In this paper, the composition and structural characteristics of purified polysaccharides are reviewed. Moreover, the immunomodulatory activities of polysaccharides are described both in vivo and in vitro. In vitro, Panax polysaccharides exert immunomodulatory functions mainly by activating macrophages, dendritic cells, and the complement system. In vivo, Panax polysaccharides can increase the immune organ indices and stimulate lymphocytes. In addition, this paper also discusses the membrane receptors and various signalling pathways of immune cells. Panax polysaccharides have many beneficial therapeutic effects, including enhancing or activating the immune response, and may be helpful in treating cancer, sepsis, osteoporosis, and other conditions. Panax polysaccharides have the potential for use in the development of novel therapeutic agents or adjuvants with beneficial immunomodulatory properties.

• 한국토양비료학회지
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• 제12권2호
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• pp.77-82
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• 1979

대두엽(大豆葉)과 비교(比較)하여 인삼절제엽(人蔘切除葉)의 위조(萎凋) 증산(蒸散) 특성(特性)을 온도별로 그리고 엽절편(葉切片)의 water potential 및 흡수특성(吸水特性)을 조사(調査)하였다. 1. 인삼엽(人蔘葉)은 대두엽(大豆葉)에 비(比)하여 얇고 초기수분함량(初期水分含量)이 크지만 탈수속도(脫水速度)는 적으면서도 영구위조점(永久萎凋点)의 수분함량(水分含量)이 높아서 (초기수분함량(初期水分含量)의 약(約) 80%, 대두(大豆)는 50%) 영구위조점(永久萎凋点)에 이르는 시간(時間)이 대두(大豆)의 반(半)에 불과(不過)하며 고온에서 더욱 단축(短縮)되었다. 2. 인삼엽(人蔘葉)은 대두엽(大豆葉)보다 증산량(蒸散量)이 적었으며 (약(約)1/4) $23^{\circ}C$보다 $33^{\circ}C$의 증산량(蒸散量)이 적어 고온에 대한 내성(耐性)이 적은 것으로 나타났다. 3. 인삼엽(人蔘葉)은 water free space가 적으나 흡수속도(吸水速度)는 (시간당 단위생중당 흡수량(吸水量)) 큰 차이(差異)가 없었고 수분결차(水分缺差)가 컸다. 4. 액침법(液浸法)에 의한 water potential은 대두엽(大豆葉)보다 컸다. 5. 이상(以上)의 결과(結果)는 인삼(人蔘)이 대두(大豆)에 비하여 수분부족(水分不足)에 약(弱)한 것을 나타내며 여름 고온에 의한 위조피해(萎凋被害)가 클 것으로 보였으며 전엽기간(展葉期間)에 수광량(受光量)을 높이고 비교적(比較的) 온도를 높게 경과하여 기공수를 증대시키어 증발산기능을 증대시켜 고온 내성(耐性)으로 키워야 하며 근권(根圈)엔 산소(酸素)가 부족(不足)되지 않는 한 수분(水分)을 충분(充分)히 공급(供給)하는 것이 좋을 것으로 보였다.

• Preventive Nutrition and Food Science
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• 제16권4호
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• pp.321-327
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• 2011

Water, methanol and ethanol extracts of ginseng leaves were assayed for total phenolics and flavonoids, ascorbic acid, cupric and ferrous ion chelating activities, 2,2-diphenyl-1-picrylhydrazyl (DPPH) radical scavenging activity, ferric reducing antioxidant power (FRAP) assay and ABTS radical cation decolourization (TEAC) assay for their antioxidant properties. The ethanol extract of ginseng leaves contained significantly (p<0.05) higher amounts of total phenolics and flavonoids (600.57 and 1701 mg/100 g) than methanol (374.43 and 1512.64 mg/100 g) and water extracts (248.30 and 680.05 mg/100 g). Among solvent extracts of ginseng leaves, the ethanol extract showed the most powerful antioxidant activities. However, the ferrous ion chelating activity of ginseng leaf extracts were lower than the cupric ion chelating ability. These differences in concentrations of key antioxidants among various solvent extracts seemed to be responsible for their differences in antioxidant activities. These results suggest that ethanol extract of ginseng leaves has the most effective antioxidant capacity compared to the methanol and water extracts tested in the present study. Thus, it can be applied for the effective extraction of functional material from ginseng leaves for the usage of pharmaceutical and/or food industries.

• Journal of Ginseng Research
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• 제42권2호
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• pp.175-182
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• 2018

Background: The impact of fungicide azoxystrobin, applied as foliar spray, on the physiological and biochemical indices and ginsenoside contents of ginseng was studied in ginseng (Panax ginseng Mey. cv. "Ermaya") under natural environmental conditions. Different concentrations of 25% azoxystrobin SC (150 g a.i./ha and 225 g a.i./ha) on ginseng plants were sprayed three times, and the changes in physiological and biochemical indices and ginsenoside contents of ginseng leaves were tested. Methods: Physiological and biochemical indices were measured using a spectrophotometer (Shimadzu UV-2450). Every index was determined three times per replication. Extracts of ginsenosides were analyzed by HPLC (Shimadzu LC20-AB) utilizing a GL-Wondasil $C_{18}$ column. Results: Chlorophyll and soluble protein contents were significantly (p = 0.05) increased compared with the control by the application of azoxystrobin. Additionally, activities of superoxide dismutase, catalase, ascorbate peroxidase, peroxidase, and ginsenoside contents in azoxystrobin-treated plants were improved, and malondialdehyde content and $O_2^-$ contents were reduced effectively. Azoxystrobin treatments to ginseng plants at all growth stages suggested that the azoxystrobin-induced delay of senescence was due to an enhanced antioxidant enzyme activity protecting the plants from harmful active oxygen species. When the dose of azoxystrobin was 225 g a.i./ha, the effect was more significant. Conclusion: This work suggested that azoxystrobin played a role in delaying senescence by changing physiological and biochemical indices and improving ginsenoside contents in ginseng leaves.

• Journal of Ginseng Research
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• 제35권4호
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• pp.449-456
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• 2011

Plant leaf cuticle is related to the prevention of moisture loss, transpiration, and diffusion of light reflection. The purpose of this study was to examine the morphological characteristics of ginseng leaves in ginseng plants resistant and susceptible to hightemperature injury (HTI) to be related with the leaf-burning. For the HTI resistant lines Yunpoong, high-temperature injury resistance (HTIR) 1, HTIR 2, and HTIR 3, and the HTI-susceptible line Chunpoong, the cuticle densities were 53.0%, 46.2%, 44.9%, 48.0%, and 17.0%; the adaxial leaf cuticle layers were 141.3, 119.7, 119.7, 159.4, and 85.0 nm in thickness; the abaxial leaf cuticle layers were 153.6, 165.8, 157.9, 199.6, and 119.4 nm in thickness; and the stomtal lengths were 21.7, 32.4, 29.4, 30.9, and $21.8{\mu}m$, respectively. All of these aspects suggest that HTI resistant lines have higher cuticle density, thickicker adaxial and abaxial leaf cuticle layers, and longer of stomta length than the HTI-susceptible line, protecting leaves from moisture loss and excessive transpiration under high temperatures to be resistant against the leaf-burning.

• Journal of Ginseng Research
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• 제39권4호
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• pp.406-413
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• 2015

Background: Pathogenesis-related 10 (PR-10) proteins are small, cytosolic proteins with a similar three-dimensional structure. Crystal structures for several PR-10 homologs have similar overall folding patterns, with an unusually large internal cavity that is a binding site for biologically important molecules. Although structural information on PR-10 proteins is substantial, understanding of their biological function remains limited. Here, we showed that one of the PgPR-10 homologs, PgPR-10.3, shares binding properties with flavonoids, kinetin, emodin, deoxycholic acid, and ginsenoside Re (1 of the steroid glycosides). Methods: Gene expression patterns of PgPR-10.3 were analyzed by quantitative real-time PCR. The three-dimensional structure of PgPR-10 proteins was visualized by homology modeling, and docking to retrieve biologically active molecules was performed using AutoDock4 program. Results: Transcript levels of PgPR-10.3 expressed in leaves, stems, and roots of 3-wk-old ginseng plantlets were on average 86-fold lower than those of PgPR-10.2. In mature 2-yr-old ginseng plants, the mRNA of PgPR-10.3 is restricted to leaves. Ginsenoside Re production is especially prominent in leaves of Panax ginseng Meyer, and the binding property of PgPR-10.3 with ginsenoside Re suggests that this protein has an important role in the control of secondary metabolism. Conclusion: Although ginseng PR-10.3 gene is expressed in all organs of 3-wk-old plantlets, its expression is restricted to leaves in mature 2-yr-old ginseng plants. The putative binding property of PgPR-10.3 with Re is intriguing. Further verification of binding affinity with other biologically important molecules in the large hydrophobic cavity of PgPR-10.3 may provide an insight into the biological features of PR-10 proteins.

• 한국작물학회지
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• 제49권2호
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• pp.94-99
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• 2004

This study was conducted to determine the effect of salinity on the growth and development of Korean ginseng (Panax ginseng C.A.Meyer) and to evaluate the inorganic ion content in Korean ginseng with different general complete fertilizer (GCF) and NaCI concentrations at two growth stages. The stem height of Korean ginseng treated with different GCF and NaCI concentrations decreased at the higher EC (2.0 dS m$^{-1}$ ), but there were no significant difference in the stem diameter, the leaf length, and the leaf width among different treatments. The root growth increased with the supply of GCF. Especially, the root growth was facilitated two times at 3.0 dS $\textrm{m}^{-1}$ as compared to control. But the root growth more sharply decreased with NaCI treatment than GCF. The $\textrm{K}^{+}$ and $\textrm{Mg}^{2+}$ content in leaves and roots increased with GCF at the early growth stage. At the late growth stage, the $\textrm{K}^{+}$ content in leaves decreased but the $\textrm{Ca}^{2+}$ and $\textrm{Mg}^{2+}$ content increased. The $\textrm{Ca}^{2+}$ and $\textrm{Mg}^{2+}$ content in roots increased but the $\textrm{K}^{+}$ content decreased. The $\textrm{Na}^{+}$ content in Korean ginseng increased sharply with NaCl treatment. The $\textrm{NO}_3^{-}$ content in leaves and $\textrm{NH}_4^{+}$ content in leaves and roots increased as GCF concentration increased. The $\textrm{NO}_3^{-}$ content in leaves, stems, and roots at the late growth stage decreased as NaCl concentration increased. The $\textrm{NH}_4^{+}$ content in leaves and roots decreased significantly at the early growth stage, but it decreased significantly in leaves and stems at the late growth stage. The root activity of Korean ginseng increased with GCF, but decreased as the EC increased with NaCl. The water potential of leaves with GCF showed no significant difference compare to control, but the water potential of leaves treated with NaCl decreased as EC increased.