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Single Somatic Embryogenesis from Transformant with Proteinase II Gene in Panax ginseng C.A. Meyer

  • Yang, Deok-Chun;Kim, Se-Young;Rho, Yeong-Deok;Kim, Moo-Sung
    • Plant Resources
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    • v.6 no.3
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    • pp.205-210
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    • 2003
  • Ginseng(Panax ginseng C.A. Meyer) is a perennial herbaceous plant which grows very slowly. It takes about 3 to 4 years from seeding to collecting the ripe seeds and the ginseng propagation is very difficult. and so, it is very difficult to breed ginseng plant. Ginseng tissue culture was started from at 1960, and ginseng commercial product by in vitro callus culture was saled, however upto now, regenerants were not planted to soil normally. Recently, plant genetic engineering to produce transgenic plants by introducing useful genes has been advanced greatly. In a present paper, transformation of ginseng plants was achieved by co-cultivation with Agrobacterium harboring the binary vector coding Proteinase-II gene, which confer resistant or tolerant to insect pests, The binary vector for transformation was constructed with disarmed Ti-plasmid and with double 35S promoter. The NPT II gene and introduced genes of the transgenic ginseng plants were successfully identified by the PCR. Especially the transgenic ginseng plants were regenerated using new techniques such as repetitive single somatic embryogenesis.

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Changes in Chemical and Sensory Characteristics of Dongchiml Juice during Fermentation with the Addition of Panax ginseng C.A. Meyer (인삼을 첨가한 동치미 쥬스 발효중 화학적 및 관능적 특성 변화)

  • Oh, Hoon-Il;Kwon, Soo-Mi;Shin, Tai-Sun
    • Journal of Ginseng Research
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    • v.20 no.3
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    • pp.307-317
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    • 1996
  • This study was undertaken to examine the effects of fresh ginseng addition on the chemical and sensory properties of dongchimi juice fermented under various conditions. The contents of free sugars in dongchimi juice during fermentation increased at the initial stage, but decreased at the palatable stage and then increased again thereafter. Free sugars were higher in dongchlml juice with 4% of ginseng addition than with Soye ginseng and without ginseng addition. Analyses of organic acid contents showed that all three groups contained relatively high amounts of citric, lactic and malic acids. It was also found that, as fermentation progressed, the amounts of tactic and acetic acids in- creased, while that of malic acid decreased at the palatable stage and increased again thereafter. Total saponins were highest in dongchimi juice fermented at 4$^{\circ}C$ with 4% of ginseng addition and panax atrlol ginsenosldes were found more than panaxadiol ginsenosides in dongchimi juice The results of sensory evaluation revealed that dongchimi juice prepared with the addition of $4^{\circ}C$ and 4% ginseng was lower in sour taste and moldy off flavor than the control, thus scoring high In total acceptability. Dongchimi juice with 4% of ginseng addition was best in most sensory characteristics.

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Browning Reaction of Fresh Ginseng (Panax ginseng C.A. Meyer) as Affected by Heating Temperature (가열온도에 따른 수삼의 갈변반응 특성)

  • 이종원;이성계
    • Journal of Ginseng Research
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    • v.19 no.3
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    • pp.249-253
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    • 1995
  • In the browning reaction of Korean ginseng, it appears that enzymatic and non-enzymatic browning reaction occurred in the initial stage of heating fresh ginseng at low temperature, and then non-enzymatic browning reaction followed in the drying period after heating. Activation energy of the browning reaction for red ginseng was about 9.0 kcal/mol. Browning reaction of red ginseng was accede- rated with an increase in steaming time, and a great extent of browning reaction occurred between 60-90 min of steaming at 10$0^{\circ}C$. Browning pigments of red ginseng were mostly water soluble subset.

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The Cancer-Preventive Potential of Panax ginseng - A Review of Human and Experimental Evidence - (인삼(Panax ginseng) 항암 효과에 관한 문헌고찰 - 실험연구와 역학연구 결과를 중심으로 -)

  • Kim, Joon-Youn;Lee, Duk-Hee;Yun, Taik-Koo;Morgan, Gareth;Vainio, Harri;Shin, Hai-Rim
    • Journal of Preventive Medicine and Public Health
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    • v.33 no.4
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    • pp.383-392
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    • 2000
  • Objective : We have reviewed the potential cancer preventive and other relevant properties of Panax ginseng C. A. Meyer, which has been traditionally used as a natural tonic in oriental countries. Data identification and study selection: Publications on Panax ginseng and its relation to cancer were obtained from the Medline database (1983-2000) and by checking reference lists to find earlier reports. The reports cover experimental models and human studies on cancer-preventive activity, carcinogenicity and other beneficial or adverse effects. In addition, possible mechanisms of chemoprevention by ginseng were also considered. Results : Published results from a cohort and two case-control studies in Korea suggest that the intake of ginseng may reduce the risk of several types of cancer. When ginseng was tested in animal models, a reduction in cancer incidence and multiplicity at various sites was noted. Panax ginseng and its chemical constituents have been tested for their inhibiting effect on putative carcinogenesis mechanisms (e.g., cell proliferation and apoptosis, immunosurveillance, angiogenesis); in most experiments inhibitory effects were found. Conclusion : While Panax ginseng C. A. Meyer has shown cancer preventive effects both in experimental models and in epidemiological studies, the evidence is currently not conclusive as to its cancer-preventive activity in humans. The available evidence warrants further research into the possible role of ginseng in the prevention of human cancer and carcinogenesis.

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Effects of some Insecticides on Growth of 2 year old Ginseng Panax ginseeng, C.A. Meyer, and Control of Root-knot nematode (몇가지 살충제의 조합이 인삼의 생육 및 근류선충 방제 효과에 미치는 영향)

  • Ahn Y.J.;Choi S.Y.;Han S.C.;Kim Y.T.
    • Korean journal of applied entomology
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    • v.20 no.4 s.49
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    • pp.223-227
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    • 1981
  • These experiments were conducted to investigate the nematicidal effects of the insecticides, Mocap(O-Ethyl-S, S-dipropyl phosphorodithioate), Carbofuran(2,3-Dihydro-2,2-dimethyl benzofuranyl ethyl carbamate), Terbufos (S-tert-buthylthio methyl O,O-diethyl phosphordithioate) and their mixtures (Mocap+carbofuran, Mocap+Terbufos, Carbofuran+Terbufos) on growth of 2year-old ginseng, Panax ginseng C.A. Meyer, and the control of root-knot nematodes. There was no evidence of plant injury from insecticide treatment of ginseng, although the rate of emergence of the treated ginseng was slightly inhibited. The insecticide treatments showed no of-flavor of ginseng plant. Terbufos and Mocap provided heifer confrol of the root-knot nematodes than carbofuran alone and their mixtures. Mixtures of the insecticides showed antagonisitic effect to the root-knot nematodes.

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Induction of Callus and Organ in Tissue Culture of Ginseng (Panax ginseng C. A. Meyer) (고려인삼의 조직배양에 의한 캘러스 및 기관분화)

  • Choe, Gwang-Tae;Kim, Myeong-Un;Sin, Hui-Seok
    • Journal of Ginseng Research
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    • v.6 no.2
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    • pp.162-167
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    • 1982
  • Calli and leaflets of ginseng (Panax ginseng C.A. Meyer) were cultured on 1/2MS media supplement. with kinetin, 2 iP, NAA, 2,4-D and IBA to assess their capacity to regenerate embryoids and organs. Root calli produced numerous embryoids and shoots in 1/2MS medium supplemented with 2 mg/l NAA and 2mg/s 2iP, and the combination of 2 iP and NAA was more effective than the combination of kinetin and NAA in induction of embryoid and shoot from root calli. Culture of leaflet in the medium supplemented with IBA resulted in profuse root regeneration.

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Studies on the Nonstarchy Polysaccharides of Korean Ginseng, Panax ginseng, C. A. Meyer II. Physicochemical propertie of pectic substances (고려인삼( Panax ginseng C A. Meyer)의 비전분성 다당류에 관한 연구 II. Pectin질의 이화학적 성질)

  • Min, Gyeong-Chan;Jo, Jae-Seon;Kim, Eun-Su
    • Journal of Ginseng Research
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    • v.8 no.2
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    • pp.105-113
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    • 1984
  • This study was conducted to investigate chemical and physical characteristics of pectin in Korean ginseng plant. The results obtained are as follows: 1, The molecular weight of the pectin in ginseng plant was in the range of 1.1-2.4x 104 and athydrouronic acid content in the pectin was 97.98%. 2. The intrinsic viscosity of pectin as well as apparent visosity of the pectin in the roots were increased with cultural period. 3. The IR spectra of ginseng pectin showed the OH stretch, C-H bending vibration and the vibration of ester group carboxyl. 4. Sugars present in galacturonic acid, glucose, arbinose, xylose, galactose and rhamnose.

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Changes of Protein Bodies in Endosperm Cells during Embryo Development of Ginseng (Panax ginseng C.A. Meyer) Seeds - Seeds with Red Seed Coat and Indehiscent Seeds - (인삼(Panax ginseng C.A. Meyer) 종자의 배발달에 따른 배유세포의 단백과립 변화 - 홍숙 및 미개갑 종자 -)

  • 유성철
    • Journal of Plant Biology
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    • v.35 no.1
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    • pp.45-51
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    • 1992
  • The changes of protein bodies in endosperm cells of both seeds with red seed coat and indehiscent seeds of Panax ginseng C.A. Meyer have been investigated in relation to the embryo development. In the early stage of seeds with red seed coat, spherical spherosomes were distributed in endosperm cells. Protein bodies were formed from vacuoles containing the storage protein. Cell organelles were hardly observed in the cytoplasm. In the late stage of the seed with red seed coat, the endosperm was filled with spherosomes and protein bodies. The protein bodies consisted of amorphous inclusions with high electron density or proteinaceous matrix with even electron density. In the seed of in dehiscence, the protein body in endosperm cells contained globoids and protein crystalloids. The globoid of protein body had a electron dense materials. Umbiliform layer was formed between embryo and endosperm. The deformation patterns of endosperm cell wall and the cellulose microfibril were observed in endosperm cells near the umbiliform layer. Umbiliform layer consisted of lipid body and autolyzed cell debris. The protein body of endosperm cell near the umbiliform layer showed various degenerative patterns, and so electron density of proteinaceous matrix was gradually decreased.reased.

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Comparative Analysis of Genetic Relationships by Quantitative Characters and Their Characteristics in Germplasm of Panax ginseng C. A. Meyer Collected in Korea and China (한국과 중국 인삼자원들의 양적 형질 특성과 유전적 유연관계 분석)

  • Song, Beom Heon;Wu, Wen Guo;Kim, Do Hyun;Chung, Jong Wook
    • Korean Journal of Medicinal Crop Science
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    • v.25 no.6
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    • pp.381-388
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    • 2017
  • Background: This study was conducted to acquire basic information on the phenotypic and genotypic characteristics of the germplasm of Panax ginseng C. A. Meyer collected from China and Korea, and identify the variations that can be utilized in ginseng breeding programs. Methods and Results: Quantitative parameters were evaluated, and used to compare and analyze on genetic polymorphisms in the germplasm. The genetic characteristics and classifications were compared and analyzed for each character. Stem length followed a normal frequency distribution ranging from 15.5 cm to 40.5 cm, with showing approximately 40% having a stem length of 20 - 30 mm. Stem diameters ranged from 2.7 mm to 11.3 mm. Stem number per plant ranged from 1 to 3; approximately 50% had a single stem, and 45% had two stems. A non-normal frequency distribution was observed for petiole number, with approximately 60% of the germplasm having 3 - 5 petioles. Petiole length exhibited a normal frequency distribution, raging from 4.5 to 10.6. Petiole angle in the germplasm ranged from $28^{\circ}$ to $89^{\circ}$ and seedstalk length ranged from 5.6 cm to 27.3 cm. Conclusions: The genetic polymorphisms identified by complete linkage clustering based on the quantitative characteristics of Panax ginseng C. A. Meyer collected from Korea and China were classified to 6 groups, namely I, II, III, IV, V, and VI with frequencies of 6.7%, 20.0%, 31.7%, 8.3%, 6.7%, and 26.7%, respectively.

고려인삼 (Panax ginseng C.A. Meyer) 의 프로테옴 분석

  • Nam, Myeong-Hui
    • Bulletin of Food Technology
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    • v.18 no.2
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    • pp.3-13
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    • 2005
  • 인삼의 효능 및 유효성분에 관한 수많은 문헌 및 연구사례가 보고 되어 있음에도 불구하고 인삼의 주성분인 ginsenosides의 합성경로나 그와 관련된 유전인자에 대하여서는 거의 밝혀져 있지 않다. 인삼의 생리적 특성규명 및 ginsenosides의 생합성 경로를 밝히기 위한 한 방법으로써 프로테옴 분석이 시도되었다. 본지에서는 고려인삼 (Panax ginseng C.A. Meyer)을 대상으로 이루어진 최근의 프로테옴 분석 및 이차 대사산물의 생산과 연관된 유전인자를 찾기 위하여 이루어진 인삼의 기능유전체 분석을 소개하고자 한다.

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