• 제목/요약/키워드: Pan-EV

검색결과 4건 처리시간 0.022초

Evaluation of Proposed Diagnostic System for Detection of Pan-enterovirus Using Reverse Transcription Nested PCR from Water Environment

  • Siwon Lee;Kyung Seon Bae;Jin-Ho Kim;Ji-Hyun Park;Ji Hye Kim;Ji-Yeon Park;Kyung-Jin Lee;Chae-Rin Jeon;Jeong-Ki Yoon;Soo-Hyung Lee;Eung-Roh Park
    • 대한의생명과학회지
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    • 제29권2호
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    • pp.81-87
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    • 2023
  • Pan-Enterovirus (Pan-EV) infects millions of children and infants worldwide every year. As severe infections have recently been reported, the need for monitoring has consequently intensified. Pan-EV is a categorical name for waterborne enteroviruses belonging to the Picornaviridae family, and includes a wide range of pathogens including Coxsackievirus (CoxV), Echovirus (EcoV) and Enterovirus (EV). In this study, we proposed an optimal RT-nested PCR method for diagnosis of various types of Pan-EV in an aquatic environment and developed a positive control. Considering detection sensitivity, specific reaction, and final identification, one condition capable of amplifying 478 bp among the four candidates in the 1st round PCR (RT-PCR) and one condition in the 2nd round PCR (nested PCR) were selected. Through the detection of nucleic acids extracted from 123 groundwater samples and the detection sensitivity test based on artificial spiking in the sample, the methods are optimal for non-disinfected water samples such as groundwater. We developed a positive control for Pan-EV detection that can be amplified to different sizes under the two conditions. Accuracy could be further improved by testing for contamination from the control group. The method proposed in this study and the positive control developed are expected to be used in monitoring Pan-EV in aquatic environments including groundwater through future research using more samples.

해남 농경지에서의 자유 수면 증발 관측과 해석 (Measurement and Analysis of Free Water Evaporation at HaeNam Paddy Field)

  • 한진수;이부용
    • 한국농림기상학회지
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    • 제7권1호
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    • pp.91-97
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    • 2005
  • BYL-EV250 증발기록계를 이용하여 해남 농경지역의 자유 수면 증발량을 관측하였고, 그 자료를 분석하여, 기상 요소와 증발량과의 관계를 분석하였다. 자연상태에서 증발량을 결정하는 기상요소의 관계를 살펴보고, 그 결과를 바람과 포차의 경험식으로 나타내었다. 이 식을 이용하여 자유 수면으로부터의 시간당 증발량을 구할 수 있었으며 수온, 기온, 습도, 풍속 같은 기상요소만으로 증발량 추정이 가능했다. 증발량 산출에 대한 신뢰성을 높이기 위해 지속적으로 수면에서의 물수지에 대한 연구가 수행되어야 할 것으로 사료된다. 증발량 관측도 다른 기상요소와 같이 매시간 관측 자료 생산 가능성을 시사했다. 부력을 이용한 측정방법은 미소 수위 변화(0.1mm 이하)를 측정 할 수 있어 대형증발계를 활용한 자동관측에 대한 신뢰성을 확보할 수 있었다. 이 결과를 바탕으로 장기간 관측된 자유수면 증발자료와 플럭스 타워에서 동시에 관측된 실제 증발산 자료의 비교분석이 현재진행 중에 있다.

이기종 무선인터넷 플랫폼의 어플리케이션 상호 호환을 위한 변환 솔루션 개발 (Development of Conversion Solutions for Interoperability of Applications on Different Mobile Internet Platforms)

  • 강경보;강동현;홍창표;류종민;이중훈;윤정한;좌정우
    • 한국콘텐츠학회논문지
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    • 제7권4호
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    • pp.1-9
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    • 2007
  • 이동통신사업자는 기존의 음성서비스에서 고속 무선인터넷 서비스를 기반으로 하는 신규 사업모델 개발을 위해 고속 무선인터넷 망을 구축하고 다기능 휴대폰을 개발하여 보급하고 있다. 국내 이동통신사업자는 무선인터넷 서비스의 활성화를 위해 cdma2000-1x, EvDo에서 HSDPA로 고속 무선인터넷망을 구축하고 MP3, MPEG, 3D 게임엔진, DMB 등 이동멀티미디어 솔루션과 팝업 창과 같이 새로운 UI를 갖는 다기능 휴대폰을 개발하여 보급하고 있다. 또한, 무선인터넷 서비스의 콘텐츠 개발을 위해 WAP, ME등의 모바일 브라우저와 WIPI, J2ME, BREW 등의 무선인터넷 플랫폼을 사용하고 있다. 그러나 이동통신 사업자별로 다른 무선인터넷 플랫폼을 사용하고 있어 콘텐츠 사업자가 무선인터넷 서비스를 사용자에게 제공하기 위해서는 서로 다른 무선인터넷 플랫폼으로 어플리케이션을 개발하여야 하는 문제가 있다. 본 논문에서는 무선인터넷 플랫폼에서 개발된 어플리케이션을 다른 무선인터넷 플랫폼에서 동작할 수 있도록 하는 어플리케이션 상호 호환을 위한 변환 솔루션을 제안한다. 제안하는 어플리케이션 상호 호환을 위한 변환 솔루션은 대표적 인 무선인터넷 플랫폼인 WIPI와 BREW 간 어플리케이션 변환을 위 해 단일패스(one-pass) 컴파일러 방식을 사용하여 개발되었다. 개발된 변환 솔루션의 성능은 무선인터넷 핵심 서비스인 게임 어플리케이션에 적용하여 API 변환율, 프로그램 성능, 변환 프로그램 크기, 변환 소요시간에 대해 확인하였다.

논벼 장.단간품종의 증발산제계수와 건물량과의 관계에 대한 연구(I) (Studies on Relations between Various Coeffcients of Evapo-Transpiration and Quantities of Dry Matters for Tall-and Short Statured Varieties of Paddy Rice)

  • 류한열;김철기
    • 한국농공학회지
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    • 제16권2호
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    • pp.3361-3394
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    • 1974
  • The purpose of this thesis is to disclose some characteristics of water consumption in relation to the quantities of dry matters through the growing period for two statured varieties of paddy rice which are a tall statured variety and a short one, including the water consumption during seedling period, and to find out the various coefficients of evapotranspiration that are applicable for the water use of an expected yield of the two varieties. PAL-TAL, a tall statured variety, and TONG-lL, a short statured variety were chosen for this investigation. Experiments were performed in two consecutive periods, a seedling period and a paddy field period, In the investigation of seedling period, rectangular galvanized iron evapotranspirometers (91cm${\times}$85cm${\times}$65cm) were set up in a way of two levels (PAL-TAL and TONG-lL varieties) with two replications. A standard fertilization method was applied to all plots. In the experiment of paddy field period, evapotanspiration and evaporation were measured separately. For PAL-TAL variety, the evapotranspiration measurements of 43 plots of rectangular galvanized iron evapotranspirometer (91cm${\times}$85cm${\times}$65cm) and the evaporation measurements of 25 plots of rectangular galvanized iron evaporimeter (91cm${\times}$85cm${\times}$15cm) have been taken for seven years (1966 through 1972), and for TONG-IL variety, the evapotranspiration measurements of 19 plots and the evaporation measurements of 12 plots have been collected for two years (1971 through 1972) with five different fertilization levels. The results obtained from this investigation are summarized as follows: 1. Seedling period 1) The pan evaporation and evapotranspiration during seedling period were proved to have a highly significant correlation to solar radiation, sun shine hours and relative humidity. But they had no significant correlation to average temperature, wind velocity and atmospheric pressure, and were appeared to be negatively correlative to average temperature and wind velocity, and positively correlative to the atmospheric pressure, in a certain period. There was the highest significant correlation between the evapotranspiration and the pan evaporation, beyond all other meteorological factors considered. 2) The evapotranpiration and its coefficient for PAL-TAL variety were 194.5mm and 0.94∼1.21(1.05 in average) respectively, while those for TONG-lL variety were 182.8mm and 0.90∼1.10(0.99 in average) respectively. This indicates that the evapotranspiration for TONG-IL variety was 6.2% less than that for PAL-TAL variety during a seedling period. 3) The evapotranspiration ratio (the ratio of the evapotranspiration to the weight of dry matters) during the seedling period was 599 in average for PAL-TAL variety and 643 for TONG-IL variety. Therefore the ratio for TONG-IL was larger by 44 than that for PAL-TAL variety. 4) The K-values of Blaney and Criddle formula for PAL-TAL variety were 0.78∼1.06 (0.92 in average) and for TONG-lL variety 0.75∼0.97 (0.86 in average). 5) The evapotranspiration coefficient and the K-value of B1aney and Criddle formular for both PAL-TAL and TONG-lL varieties showed a tendency to be increasing, but the evapotranspiration ratio decreasing, with the increase in the weight of dry matters. 2. Paddy field period 1) Correlation between the pan evaporation and the meteorological factors and that between the evapotranspiration and the meteorological factors during paddy field period were almost same as that in case of the seedling period (Ref. to table IV-4 and table IV-5). 2) The plant height, in the same level of the weight of dry matters, for PAL-TAL variety was much larger than that for TONG-IL variety, and also the number of tillers per hill for PAL-TAL variety showed a trend to be larger than that for TONG-IL variety from about 40 days after transplanting. 3) Although there was a tendency that peak of leaf-area-index for TONG-IL variety was a little retarded than that for PAL-TAL variety, it appeared about 60∼80 days after transplanting. The peaks of the evapotranspiration coefficient and the weight of dry matters at each growth stage were overlapped at about the same time and especially in the later stage of growth, the leaf-area-index, the evapotranspiration coefficient and the weight of dry matters for TONG-IL variety showed a tendency to be larger then those for PAL-TAL variety. 4) The evaporation coefficient at each growth stage for TONG-IL and PAL-TALvarieties was decreased and increased with the increase and decrease in the leaf-area-index, and the evaporation coefficient of TONG-IL variety had a little larger value than that of PAL-TAL variety. 5) Meteorological factors (especially pan evaporation) had a considerable influence to the evapotranspiration, the evaporation and the transpiration. Under the same meteorological conditions, the evapotranspiration (ET) showed a increasing logarithmic function of the weight of dry matters (x), while the evaporation (EV) a decreasing logarithmic function of the weight of dry matters; 800kg/10a x 2000kg/10a, ET=al+bl logl0x (bl>0) EV=a2+b2 log10x (a2>0 b2<0) At the base of the weight of total dry matters, the evapotranspiration and the evaporation for TONG-IL variety were larger as much as 0.3∼2.5% and 7.5∼8.3% respectively than those of PAL-TAL variety, while the transpiration for PAL-TAL variety was larger as much as 1.9∼2.4% than that for TONG-IL variety on the contrary. At the base of the weight of rough rices the evapotranspiration and the transpiration for TONG-IL variety were less as much as 3.5% and 8.l∼16.9% respectively than those for PAL-TAL variety and the evaporation for TONG-IL was much larger by 11.6∼14.8% than that for PAL-TAL variety. 6) The evapotranspiration coefficient, the evaporation coefficient and the transpiration coefficient and the transpiration coefficient were affected by the weight of dry matters much more than by the meteorological conditions. The evapotranspiratioa coefficient (ETC) and the evaporation coefficient (EVC) can be related to the weight of dry matters (x) by the following equations: 800kg/10a x 2000kg/10a, ETC=a3+b3 logl0x (b3>0) EVC=a4+b4 log10x (a4>0, b4>0) At the base of the weights of dry matters, 800kg/10a∼2000kg/10a, the evapotranspiration coefficients for TONG-IL variety were 0.968∼1.474 and those for PAL-TAL variety, 0.939∼1.470, the evaporation coefficients for TONG-IL variety were 0.504∼0.331 and those for PAL-TAL variety, 0.469∼0.308, and the transpiration coefficients for TONG-IL variety were 0.464∼1.143 and those for PAL-TAL variety, 0.470∼1.162. 7) The evapotranspiration ratio, the evaporation ratio (the ratio of the evaporation to the weight of dry matters) and the transpiration ratio were highly affected by the meteorological conditions. And under the same meteorological condition, both the evapotranspiration ratio (ETR) and the evaporation ratio (EVR) showed to be a decreasing logarithmic function of the weight of dry matters (x) as follows: 800kg/10a x 2000kg/10a, ETR=a5+b5 logl0x (a5>0, b5<0) EVR=a6+b6 log10x (a6>0 b6<0) In comparison between TONG-IL and PAL-TAL varieties, at the base of the pan evaporation of 343mm and the weight of dry matters of 800∼2000kg/10a, the evapotranspiration ratios for TONG-IL variety were 413∼247, while those for PAL-TAL variety, 404∼250, the evaporation ratios for TONG-IL variety were 197∼38 while those for PAL-TAL variety, 182∼34, and the transpiration ratios for TONG-IL variety were 216∼209 while those for PAL-TAL variety, 222∼216 (Ref. to table IV-23, table IV-25 and table IV-26) 8) The accumulative values of evapotranspiration intensity and transpiration intensity for both PAL-TAL and TONG-IL varieties were almost constant in every climatic year without the affection of the weight of dry matters. Furthermore the evapotranspiration intensity appeared to have more stable at each growth stage. The peaks of the evapotranspiration intensity and transpiration intensity, for both TONG-IL and PAL-TAL varieties, appeared about 60∼70 days after transplanting, and the peak value of the former was 128.8${\pm}$0.7, for TONG-IL variety while that for PAL-TAL variety, 122.8${\pm}$0.3, and the peak value of the latter was 152.2${\pm}$1.0 for TONG-IL variety while that for PAL-TAL variety, 152.7${\pm}$1.9 (Ref.to table IV-27 and table IV-28) 9) The K-value in Blaney & Criddle formula was changed considerably by the meteorological condition (pan evaporation) and related to be a increasing logarithmic function of the weight of dry matters (x) for both PAL-TAL and TONG-L varieties as follows; 800kg/10a x 2000kg/10a, K=a7+b7 logl0x (b7>0) The K-value for TONG-IL variety was a little larger than that for PAL-TAL variety. 10) The peak values of the evapotranspiration coefficient and k-value at each growth stage for both TONG-IL and PAL-TAL varieties showed up about 60∼70 days after transplanting. The peak values of the former at the base of the weights of total dry matters, 800∼2000kg/10a, were 1.14∼1.82 for TONG-IL variety and 1.12∼1.80, for PAL-TAL variety, and at the base of the weights of rough rices, 400∼1000 kg/10a, were 1.11∼1.79 for TONG-IL variety and 1.17∼1.85 for PAL-TAL variety. The peak values of the latter, at the base of the weights of total dry matters, 800∼2000kg/10a, were 0.83∼1.39 for TONG-IL variety and 0.86∼1.36 for PAL-TAL variety and at the base of the weights of rough rices, 400∼1000kg/10a, 0.85∼1.38 for TONG-IL variety and 0.87∼1.40 for PAL-TAL variety (Ref. to table IV-18 and table IV-32) 11) The reasonable and practicable methods that are applicable for calculating the evapotranspiration of paddy rice in our country are to be followed the following priority a) Using the evapotranspiration coefficients based on an expected yield (Ref. to table IV-13 and table IV-18 or Fig. IV-13). b) Making use of the combination method of seasonal evapotranspiration coefficient and evapotranspiration intensity (Ref. to table IV-13 and table IV-27) c) Adopting the combination method of evapotranspiration ratio and evapotranspiration intensity, under the conditions of paddy field having a higher level of expected yield (Ref. to table IV-23 and table IV-27). d) Applying the k-values calculated by Blaney-Criddle formula. only within the limits of the drought year having the pan evaporation of about 450mm during paddy field period as the design year (Ref. to table IV-32 or Fig. IV-22).

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