• Animal Bioscience
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• v.34 no.7
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• pp.1210-1220
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• 2021

Objective: Unlike mammals, goose fatty liver shows a strong tolerance to fatty acids without obvious injury. Stearyl-coenzyme A desaturase 1 (SCD1) serves crucial role in desaturation of saturated fatty acids (SAFs), but its role in the SAFs tolerance of goose hepatocytes has not been reported. This study was conducted to explore the role of SCD1 in regulating palmitic acid (PA) tolerance of goose primary hepatocytes. Methods: 3-(4, 5-dimethylthiazol-2-yl)-2, 5-diphenyltetrazolium bromide was examined to reflect the effect of PA on hepatocytes viability, and quantitative polymerase chain reaction was used to detect the mRNA levels of several genes related to endoplasmic reticulum (ER) stress, inflammation, and apoptosis, and the role of SCD1 in PA tolerance of goose hepatocytes was explored using RNA interfere. Results: Our results indicated that goose hepatocytes exhibited a higher tolerant capacity to PA than human hepatic cell line (LO2 cells). In goose primary hepatocytes, the mRNA levels of fatty acid desaturation-related genes (SCD1 and fatty acid desaturase 2) and fatty acid elongate enzyme-related gene (elongase of very long chain fatty acids 6) were significantly upregulated with 0.6 mM PA treatment. However, in LO2 cells, expression of ER stress-related genes (x box-binding protein, binding immunoglobulin protein, and activating transcription factor 6), inflammatory response-related genes (interleukin-6 [IL-6], interleukin-1β [IL-1β], and interferon-γ) and apoptosis-related genes (bcl-2-associated X protein, b-cell lymphoma 2, Caspase-3, and Caspase-9) was significantly enhanced with 0.6 mM PA treatment. Additionally, small interfering RNA (siRNA) mediated downregulation of SCD1 significantly reduced the PA tolerance of goose primary hepatocytes under the treatment of 0.6 mM PA; meanwhile, the mRNA levels of inflammatory-related genes (IL-6 and IL-1β) and several key genes involved in the phosphoinositide 3-kinase (PI3K)/protein kinase B (AKT), forkhead box O1 (FoxO1), mammalian target of rapamycin and AMPK pathways (AKT1, AKT2, FoxO1, and sirtuin 1), as well as the protein expression of cytochrome C and the apoptosis rate were upregulated. Conclusion: In conclusion, our data suggested that SCD1 was involved in enhancing the PA tolerance of goose primary hepatocytes by regulating inflammation- and apoptosis-related genes expression.

• Korean Journal of Soil Science and Fertilizer
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• v.24 no.2
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• pp.144-151
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• 1991

Composition and unsaturation ratio of fatty acids composing phospholipid which is the main constituent of biological membranes are known to be related to the response of plant to cold stress. In order to investigate a relationship between fatty acid unsaturation ratio of phospholipid and the range of cold tolerance of rice cultivars obtained in the field experiment, the lipid from the leaves of 32 rice cultivars were isolated and the fatty acid composition of phospholipid was determined by gas chromatography. In this experiment, composition and unsaturation ratio of fatty acids composing phopholipid on rice plants grown under natural condition and treated with chilling temperature were compared with the cold tolerance in the field for rice cultivars. The results obtained were summarized as follows ; 1. The phospholipids isolated from the leaves of 32 rice cultivars both grown under natural condition and chilling treatment contained palmitic, linoleic and linolenic acid as major components and palmitoleic, stearic and oleic acid as minor components. 2. The content of palmitic acid, a saturated fatty acid, was reduced in rice seedlings subjected to chilling treatment for 3 days at $15^{\circ}C$, and contents of linoleic and linolenic acid, unsaturated fatty acids, were increased. 3. The unsaturation ratio of fatty acid was increased by chilling treatment, and was high in cold-tolerant cultivars but low in cold-susceptible cultivars and the results were correlated with the range of cold tolerance investigated in the field. 4. The content of palmitic acids among fatty acids composing phospholipid was reduced and that of linolenic acid was increased by hardening treatment. 5. The unsaturation ratio of fatty acid was increased 18~24% by hardening treatment compared to control.

• Journal of the Korean Society of Food Science and Nutrition
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• v.22 no.5
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• pp.576-580
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• 1993

An attempt was made to investigate the chemical compositions of the three plant types such as runner type(Yeoju landrace), semi-Spanish type(Suweon 15ho) and Spanish type(Wasedairu) of peanut. The proximate compositions were lipid 44.25~46.48% and protein 22.54~25.42% in various plant types of peanut, respectively. And the contents of purified free and bond lipids were 43.71~45.44% and 1.74~3.12%, respectively. The predominant fatty acids were oleic, linoleic, palmitic and arachidic acid in the free lipid, and linoleic, oleic and palmitic acid in t도 bond lipid. Pattern of 16 amino acid compositions in three plant types of peanut was shown to be similarity. Major amino acid were glutamic acid(75.30~104.66mg/g), arginine(57.30~74.27mg/g), aspartic acid(41.44~63.05mg/g) and leucine(30.80~39.00mg/g). The richest mineral contained in three plant types of peanut was noticed to be K and followed by Mg, Ca and Na. The lead content was below the authorized tolerance limits.

• KOREAN JOURNAL OF CROP SCIENCE
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• v.55 no.3
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• pp.239-244
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• 2010

The purpose of this study was to identify mungbean (Vigna radiata L.) cultivars with appropriate characteristics for labor saving culture (whole crop feeding and combine harvesting), and to investigate seed quality in the southern South Korea. Cultivar Dahyeon exhibited strong lodging resistance, excellent disease tolerance, and greater pod numbers per plant resulting in higher yield. Cultivar Owool and Keumseong, the two most common mungbean cultivars in Korea, exhibited lower yield than Dahyeon due to weaker disease tolerance or lower pod numbers per plant. Cultivar Samgang demonstrated higher seed starch content, Jangan, Nampyeong, and Keumseong exhibited higher crude protein content, and Sohyeon exhibited higher vitexin and isovitexin contents. However, no statistical differences were found among the cultivars in crude fat content. Unsaturated fatty acid ranged from 51.8 to 57.2%, with saturated fatty acid ranging from 36.2 to 40.3%. We detected five unsaturated fatty acids including linoleic acid (36.1 to 38.6%), linolenic acid (10.3 to 14.7%), and oleic acid (2.7 to 4.6%), and seven saturated fatty acids including palmitic acid (28.7 to 30.9%), stearic acid (2.9 to 4.1%), and arachidic acid (1.5 to 3.7%). There were significant differences between the cultivars in amylogram properties of seeds: the Nampyeong cultivar exhibited a lower gelatinization temperature; Dahyeon was higher in peak viscosity and breakdown; and Sohyeon, Nampyeong, and Dahyeon were lower in setback.

• KOREAN JOURNAL OF CROP SCIENCE
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• v.36 no.5
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• pp.429-444
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• 1991

Cold stress influence plant growth through a wide range of growth characters. Adverse effects of low temperature to plant growth come from results of colligative and complex physiological responses to cold stress. To evaluate more exactly cold tolerance of crop plant, it is needed to observe physiological changes induced by cold stress and to analyze relationships between intraspecific variations in physiological factors related to cold tolerance and the extent of cold tolerance in the field. Therefore, the composition and unsaturation ratio of fatty acids in phospholipid, a constituent of membrane, the transition-temperature in respiratory activity of mitochodria, the chlorophyll fluorescence as a factor related to photosynthesis were investigated in rice plant and data on these factors were compared with the degree of cold tolerance obtained in the field experiment. Also, effects of hardening and Mn++ treatment were evaluated as a method to reduce chilling injuries. The unsaturation ratio of fatty acids, whether rice plants were grown in a natural condition or under the chilling stress, was higher in the cold- tolerant varieties and was significantly correlated with the degree of cold tolerance (1-9) observed in the field experiment. And it was also increased by chilling treatment or hardening treatment, due to a reduction in palmitic acid content and an increase in linolenic acid content. The transition-temperature of respiratory activity of mitochodria isolated from etiolated rice seedlings ($25^{\circ}C$, two week-grown in the dark), was correlated with the degree of cold tolerance in the field, cold -tolerant varieties showing a lower transition-temperature. It was not influenced by growth stages. The intensity of chlorophyll fluorescence was highly correlated with the degree of cold tolerance, cold-tolerant varieties having a higher fluorescence intensity. By foliar application of Mn, the transition-temperature of respiratory activity was lowered as much as 0-2$^{\circ}C$ in all tested varieties. Soil application of Mn induced more significant effect in cold-susceptible varieties with a possibility of reducing chilling injuries. On the whole, there were high correlationships among the degree of cold tolerance, the unsaturation ratio of fatty acids in phospholipid, the transition- temperature of respiratory activity and chlorophyll fluorescence except for a few varieties. The transition- temperature of respiratory activity appeared to be negatively correlated with the unsaturation ratio of fatty acids. and the chlorophyll fluorescence to be positively correlated with the unsaturation ratio. This implies that these physical and physiological factors were very closely related to cold tolerance and can be used as an effective index of the evaluation of cold tolerance of crop plant. But other factors as well as three factors discussed above are needed to be considered colligatively and altogether with a systematic analysis for the more exact evaluation of cold tolerance. in rice cultivars. in rice cultivars.

• Nutrition Research and Practice
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• v.7 no.4
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• pp.294-301
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• 2013

In this study, we examined the hepatic anti-steatosis activity of carnosic acid (CA), a phenolic compound of rosemary (Rosmarinus officinalis) leaves, as well as its possible mechanism of action, in a high-fat diet (HFD)-fed mice model. Mice were fed a HFD, or a HFD supplemented with 0.01% (w/w) CA or 0.02% (w/w) CA, for a period of 12 weeks, after which changes in body weight, blood lipid profiles, and fatty acid mechanism markers were evaluated. The 0.02% (w/w) CA diet resulted in a marked decline in steatosis grade, as well as in homeostasis model assessment of insulin resistance (HOMA-IR) index values, intraperitoneal glucose tolerance test (IGTT) results, body weight gain, liver weight, and blood lipid levels (P < 0.05). The expression level of hepatic lipogenic genes, such as sterol regulating element binding protein-1c (SREBP-1c), liver-fatty acid binding protein (L-FABP), stearoyl-CoA desaturase 1 (SCD1), and fatty acid synthase (FAS), was significantly lower in mice fed 0.01% (w/w) CA and 0.02% (w/w) CA diets than that in the HFD group; on the other hand, the expression level of ${\beta}$-oxidation-related genes, such as peroxisome proliferator-activated receptor ${\alpha}$ (PPAR-${\alpha}$), carnitine palmitoyltransferase 1 (CPT-1), and acyl-CoA oxidase (ACO), was higher in mice fed a 0.02% (w/w) CA diet, than that in the HFD group (P < 0.05). In addition, the hepatic content of palmitic acid (C16:0), palmitoleic acid (C16:1), and oleic acid (C18:1) was significantly lower in mice fed the 0.02% (w/w) CA diet than that in the HFD group (P < 0.05). These results suggest that orally administered CA suppressed HFD-induced hepatic steatosis and fatty liver-related metabolic disorders through decrease of de novo lipogenesis and fatty acid elongation and increase of fatty acid ${\beta}$-oxidation in mice.

• Food Science and Preservation
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• v.22 no.2
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• pp.218-224
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• 2015

Crickets have been used as crude drug for treating fever and hypertension in East Asia. This study was carried out to investigate the quality characteristics such as the microbial and nutrient contents of crickets (Gryllus bimaculatus) prepared with various processing conditions for use as food. These conditions included the lyophilization process (LP), hot-air process (HP, $90^{\circ}C$ for 7 hr), roasting process (RP, $160^{\circ}C$ for 40 min), and frying process (FP, $180^{\circ}C$ for 30 sec). The total bacterial population of the crickets was 5~7 log CFU/g, but Coliform and E. coli were not detected. The major fatty acids in all the samples were palmitic acid (C16:0), oleic acid (C18:1), and linoleic acid (C18:2). The level of polyunsaturated fatty acids was highest (63.55 g/100 g) in the LP-processed crickets. As for the amino acid content, the glutamic acid level was highest in all the samples, and the RP and FP decreased by 12.01% and 53.88%, respectively, compared to that of the LP. The mineral contents were highest in the LP-processed crickets. Hg was detected at about 1.0 ppb in all the samples, but its level was lower than the residue tolerance level in the Korean Food Code. Such conditions should be considered to better understand the quality characteristics of crickets in food processing.

• Korean Journal of Weed Science
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• v.8 no.1
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• pp.53-58
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• 1988

This study was carried out to determine effect of butachlor [N.-(buthoxymethyl)-2-chloro-N-(2,6-diethylphenyl) acetamide] and bialaphos [2-amino-4(hydroxy)(methyl) phosphionyl] butyryl-alanylalanine sodium salt on the germination of tobacco seed, induction and growth of callus from tobacco. Further, fatty acids and ammonia content of tobacco calli were determined. Bialaphos had no effect on tobacco seed germination, but the growth of seedling was markedly affected by an application of 10 ppm bialaphos. However, regardless of varieties tested, tobacco seed germination was completely inhibited by $5{\times}10^{-5}M$ of butachlor. At an application of $5{\times}10^{-5}M$ butachlor, tobacco seeds were to some extent germinated and showed further growth. Hyangcho among varieties tested, showed the most tolerant response to butachlor. In induction of callus from various tobacco varieties and their growth, aromatic type of tobacco varieties exhibited the most tolerance against bialaphos. However, no distinct varietal differences were determined in the treatment of butachlor. The major fatty acids identified in tobacco calli were palmitic, oleic and linoleic acid. No marked difference in terms of fatty acids was observed among tobacco varieties used, but it was observed that there was the higher ratio of quantity in unsaturated fatty acids over saturated one, bialaphos treatment accumulated about 9 times higher ammonia content than that of the untreated control, giving an evidence that bialaphos might inhibit glutamine synthetase activity.