• Journal of the Korean Applied Science and Technology
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• v.32 no.2
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• pp.348-361
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• 2015

To reduce the effects of greenhouse gas (GHG) emissions, the government has announced the special platform of technologies as parts of an effort to minimize global climate change, and the government distributed biodiesel since 2006 as the further efforts. Although there are some debates about some quality specifications and unbalanced of source (44% from palm oil), more than 400kton/year of biodiesel was produced in 2013. Moreover the amounts will be increased when the RFS is activated. To solve the unbalanced situation and to achieve the diversity of feeds, it is essential that many researches should be considered. Especially, free and bond glycerines are one of the important properties seriously affected to the combustion system in vehicle & cold properties. Previous method (KS M 2412) couldn't cover the biodiesel derived from lauric oil($C_{12:0}$) such as PKO (Palm Kernel Oil), Coconut oil because those compositions are lighter than other conventional biodiesel sources. In this study, we review the existed method and figure out the factors should improve to analysis the glycerine from PKO and Coconut oil biodiesel. Modifying the analysis conditions to enhance the resolution and change the internal standards to avoid the overlapped- peaks between Capric acid ME ($C_{10:0}$) and standard#1(1,2,4-butantriol). From this revised method, we could solve the restrictions of previous methods. And check the possibility of new method to analyze the glycerine in biodiesel regardless of sources.

• Journal of Microbiology and Biotechnology
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• v.13 no.1
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• pp.64-69
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• 2003

Pseudomonas chlororaphis HS21 was isolated from a soil sample and found to produce medium-chain-length polyhydroxyalkanoates (MCL-PHAs) using palm kernel oil (PKO) as the sole carbon source. Up to 3.3 g/1 dry cell weight containing $45\%$ MCL-PHA was produced, when the strain was grown for 21 h in a jar fermentor culture containing 5 g/1 PKO. The polymer produced from PKO consisted of unsaturated monomers of $7.3\%$ 3-hydroxy-5-cis-tetradecenoate and $2.3\%$ 3-hydroxy-5,8,-cis, cis-tetradecadienoate as well as saturated even-carbon number monomers ranging from $C_6\;to\;C_14$, as determined by GC and El GC/MS The PHA was a transparent, sticky material at room temperature. A differential scanning calorimetric analysis revealed that the polymer was amorphous with a $-44^{\circ}C$ glass transition temperature. The number average molecular weight and polydispersity index of the PHA were 83,000 and 1.53, respectively. Although the PHA was practically biodegradable, its degradability was lower than that of poly(3-hydroxyoctanoate) based on a comp:trison of the clear zones formed by growing PHA depolymerase-producing bacteria on an agar plate containing the respective polymers.