• 한국광학회지
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• 제12권3호
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• pp.205-211
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• 2001

본 논문에서는 최근 주목받고 있는 장파장 EDFA(Erbium-Doped Fiber Amplifer)에서의 성능 분석, 특히 전력변환률(Power Conversion Efficiency)의 예측을 위한 밀도반전이 고정된 상태에서의 black box 모델을 유도하고 이득이 평탄화된 장파장 EDFA의 요구되는 펌프파워측면에서 수치해석과 실험을 통하여 유도된 모델의 유용성을 증명하였다. 모델을 통한 펌프파워의 예측치는 실험오차 범위내에서 1.9%이내의 정확성을 보였다. 밀도반전이 고정된 상태에서의 black box 모델은 일반적으로 980nm, 1480m, 펌핑의 경우 모두 적용이 가능하며 EDF의 3가지 이상의 길이에 대하여 입력 펌프파워와 입력/출력 신호파워의 관계를 측정하여 밀도반전이 일정한 상태에서 다른 동작점의 성능을 예측할 수 있었다. 또한 단파장 EDFA에서는 손실의 효과가 크게 나타나지 않아 해석적인 해와 일치하는 결과가 알려져 있었으나 이에 비해 장파장 EDFA에서는 여러 가지 손실의 효과가 더욱 크게 작용하게 되며 특히 paired ion의 효과로 인한 손실로써 비선형적인 관계식이 유도됨을 보였다.

• 식물조직배양학회지
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• 제25권6호
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• pp.453-475
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• 1998

During the 100 years since the initial discovery of meiotic phenomenon many brilliant aspects have been elucidated, but further researches based on light microscopy alone as an experimental tool have been found to have some limits and shortcomings. By the use of electron microscopy and armed with the advanced knowledges on modern genetics and biochemistry it has been possible to applu molecular technology in gaining information on the detailed aspects of meiosis. As synapsis takes place, a three-layered proteinous structure called the synatonemal complex starts to form in the space between the homologous chromosomes. To be more precise, it begins to form along the paired chromosomes early in the prophase I of meiotic division. The mechanism that leads to precise point-by-point pairing between homologous chromocomes division. The mechamism that leads to precise point-by-point pairing between homologous chromosomes remains to be ascertained. Several items of information, however, suggest that chromsome alignment leading to synapsis may be mediated somehow by the nuclear membrane. Pachytene bivalents in eukaryotes are firmly attached to the inner niclear membrane at both termini. This attached begins with unpaired leptotene chromosomes that already have developed a lateral element. Once attached, the loptotene chromosomes begin to synapse. A number of different models have been proposed to account for genetic recombination via exchange between DNA strands following their breakage and subsequent reunion in new arrangement. One of the models accounting for molecular recombination leading to chromatid exchange and chiasma formation was first proposed in 1964 by Holliday, and 30 years later still a modified version of his model is favored. Nicks are made by endomuclease at corresponding sites on one strant of each DNA duplex in nonsister chromatid of a bivalent during prophase 1 of meiosis. The nicked strands loop-out and two strands reassociate into an exchanged arrangement, which is sealed by ligase. The remaining intact strand of each duplex is nicked at a site opposite the cross-over, and the exposed ends are digested by exonuclease action. Considerable progress has been made in recent years in the effort to define the molecular and organization features of the centromere region in the yeast chromosome. Centromere core region of the DNA duplex is flanked by 15 densely packed nucleosomes on ons side and by 3 packed nucleosomes on the other side, that is, 2000 bp on one side and 400 400 bp in the other side. All the telomeres of a given species share a common DNA sequence. Two ends of each chromosome are virtually identical. At the end of each chromosome there exist two kinds of DNA sequence" simple telpmeric sequences and telpmere-associated sequencies. Various studies of telomere replication, function, and behabior are now in progress, all greatly aided by molecular methods. During nuclear division in mitosis as well as in meiosis, the nucleili disappear by the time of metaphase and reappear during nuclear reorganizations in telophase. When telophase begins, small nucleoli form at the NOR of each nucleolar-organizing chromosome, enlarge, and fuse to form one or more large nucleoli. Nucleolus is a special structure attached top a specific nucleolar-organizing region located at a specific site of a particular chromosome. The nucleolus is a vertical factory for the synthesis of rRNAs and the assenbly of ribosome subunit precursors.sors.

• 대한화학회지
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• 제42권4호
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• pp.416-421
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• 1998

귀금속-티아크라운에테르 착물들의 역상 이온쌍 고성능 액체크로마토그래피(RPIP-HPLC)용리 거동에서 이온쌍 시약의 농도와 리간드 종류 효과를 연구하였다. 귀금속 이온 분석의 선택성은 사용한 티아크라운에테르의 고리를 구성하는 원자들의 수가 작을수록 좋았고, 귀금속-티아크라운에테르 착물의 용리 메카니즘은 이동상 중 이온쌍 시약인 도데실술폰산나트륨염(SDS)의 농도가 10 mM 이하일 경우 이온쌍 형성 메카니즘으로, 10 mM 이상일 경우 미쎌 형성 메카니즘으로 설명되었다. 결론적으로 몇 가지 귀금속-티아크라운에테르 착물들을 최적 조건에서 성공적으로 분리하였고, 흑백사진 정착액 중 Ag(Ⅰ) 이온 분리와 정량에 유용함을 증명하였다.

• Molecules and Cells
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• 제42권5호
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• pp.426-439
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• 2019

Many small RNAs (sRNAs) regulate gene expression by base pairing to their target messenger RNAs (mRNAs) with the help of Hfq in Escherichia coli. The sRNA DsrA activates translation of the rpoS mRNA in an Hfq-dependent manner, but this activation ability was found to partially bypass Hfq when DsrA is overproduced. The precise mechanism by which DsrA bypasses Hfq is unknown. In this study, we constructed strains lacking all three rpoS-activating sRNAs (i.e., ArcZ, DsrA, and RprA) in $hfq^+$ and $Hfq^-$ backgrounds, and then artificially regulated the cellular DsrA concentration in these strains by controlling its ectopic expression. We then examined how the expression level of rpoS was altered by a change in the concentration of DsrA. We found that the translation and stability of the rpoS mRNA are both enhanced by physiological concentrations of DsrA regardless of Hfq, but that depletion of Hfq causes a rapid degradation of DsrA and thereby decreases rpoS mRNA stability. These results suggest that the observed Hfq dependency of DsrA-mediated rpoS activation mainly results from the destabilization of DsrA in the absence of Hfq, and that DsrA itself contributes to the translational activation and stability of the rpoS mRNA in an Hfq-independent manner.

• 한국미생물생명공학회:학술대회논문집
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• 한국미생물생명공학회 2001년도 Proceedings of 2001 International Symposium
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• pp.83-89
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• 2001

Recent advances in the structural and molecular biology uncovered that a set of translation factors resembles a tRNA shape and, in one case, even mimics a tRNA function for deciphering the genetic ：ode. Nature must have evolved this 'art' of molecular mimicry between protein and ribonucleic acid using different protein architectures to fulfill the requirement of a ribosome 'machine'. Termination of protein synthesis takes place on the ribosomes as a response to a stop, rather than a sense, codon in the 'decoding' site (A site). Translation termination requires two classes of polypeptide release factors (RFs)： a class-I factor, codon-specific RFs (RFI and RF2 in prokaryotes; eRFI in eukaryotes), and a class-IT factor, non-specific RFs (RF3 in prokaryotes; eRF3 in eukaryotes) that bind guanine nucleotides and stimulate class-I RF activity. The underlying mechanism for translation termination represents a long-standing coding problem of considerable interest since it entails protein-RNA recognition instead of the well-understood codon-anticodon pairing during the mRNA-tRNA interaction. Molecular mimicry between protein and nucleic acid is a novel concept in biology, proposed in 1995 from three crystallographic discoveries, one, on protein-RNA mimicry, and the other two, on protein-DNA mimicry. Nyborg, Clark and colleagues have first described this concept when they solved the crystal structure of elongation factor EF- Tu：GTP：aminoacyl-tRNA ternary complex and found its overall structural similarity with another elongation factor EF-G including the resemblance of part of EF-G to the anticodon stem of tRNA (Nissen et al. 1995). Protein mimicry of DNA has been shown in the crystal structure of the uracil-DNA glycosylase-uracil glycosylase inhibitor protein complex (Mol et al. 1995; Savva and Pear 1995) as well as in the NMR structure of transcription factor TBP-TA $F_{II}$ 230 complex (Liu et al. 1998). Consistent with this discovery, functional mimicry of a major autoantigenic epitope of the human insulin receptor by RNA has been suggested (Doudna et al. 1995) but its nature of mimic is. still largely unknown. The milestone of functional mimicry between protein and nucleic acid has been achieved by the discovery of 'peptide anticodon' that deciphers stop codons in mRNA (Ito et al. 2000). It is surprising that it took 4 decades since the discovery of the genetic code to figure out the basic mechanisms behind the deciphering of its 64 codons.

• 방송공학회논문지
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• 제13권1호
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• pp.53-61
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• 2008

본 논문에서는 전방향 안전성(forward-secrecy)을 보장하는 공개키 브로드캐스트 암호 기법을 제안한다. 공개키 브로드캐스트 암호는 공개키를 이용하여 구성원 누구나 메시지를 전송할 수 있고, 탈퇴자 그룹을 효율적으로 배제(revocation)할 수 있는 기법이다. 여기에 전방향 안전성을 보장하려는데, 전방향 안전성은 사용자의 비밀키가 노출되더라도 그 노출된 시점 이전의 암호문을 쉽게 복호화 할 수 없도록 하는 것이다. 이러한 기능이 없다면 권한 없는 수신자가 과거의 방송을 수집하고 이후 정당한 비밀키를 받아서 과거의 방송을 복호화할 수 있는 문제가 발생한다. 전방향 안전성은 특히 유료 방송 등의 환경에서 요구된다. 본 논문에서 제안되는 기법은 2005년 Boneh-Boyen-Goh가 제시한 계층구조의 신원 기반 암호기법을 변형하여 설계된다. 먼저 BBG기법을 사용하여 새로운 공개키 브로드캐스트 암호기법을 설계하고, 다시 BBG 기법에서 사용된 하위레벨 비밀키 생성 알고리즘을 사용하여 전방향 안전성을 부여한다. 제안되는 기법은 타원곡선 위의 페어링(pairing)을 이용하여 설계되며, 전체 사용자 n에 대하여 $O(\sqrt{n})$ 사이즈의 통신량과 비밀키 저장량을 가진다. 특히 비밀키 저장량은 탈퇴자 수가 증가할수록 줄어드는 장점을 가진다. 통신량이 중요한 환경에서는 이전에 제시된 기법보다 본 논문에서 제안된 기법을 사용하는 것이 더 바람직한데, 이는 통신량은 동일하지만 비밀키 저장량이 더 적기 때문이다. 제안된 기법은 Bilinear Diffie-Hellman Exponent 가정 하에서 선택 암호문 공격에 안전하도록 설계되며, 그 증명은 랜덤 오라클을 사용하지 않는다.