• Korean Journal of Veterinary Research
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• v.36 no.4
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• pp.809-819
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• 1996

This study was conducted to identify the effects of caffeine or combinations of caffeine and iron or vitamin E on the lipid and protein components in the MDBK(Mardin-Darby Bovine Kidney) cells. For the In vitro test, MDBK cells in ${\alpha}$-MEM(Minimum Essential Medium) were divided into 4 treatment groups according to drug types and dosages as follows; the control(group A), group B was treated with 0.3mM caffeine, group C was treated with 0.3mM caffeine and 0.3mM ferric chloride, group D was treated with 0.3mM caffeine and 0.3mM vitamin E. Those groups were further divided into 5 subgroups according to the time lapsed(control, 4hrs, 8hrs, 24hrs and 48hrs lapsed group). The concentrations of the carbonyl group and malondialdehyde(MDA) and the patterns of the SDS-PAGE(Sodium Dodecyl Sulfate-Polyacrylamide Gel Electrophoresis) and fatty acid compositions were analyzed to determine the oxidative damages and metabolic changes on the lipid and protein components in the MDBK cells. The results obtained from this study were summarized as follows; 1. The concentrations of carbonyl group and malondialdehyde in MDBK cells of group C were significantly higher(p<0.01) in comparison to the control, and increased according to the time lapsed. But the results of groups B and D were little different in comparison to the group C. 2. As the analytical results of fatty acid compositions in MDBK cells, the proportions of palmitoleic acid and linoleic acid in groups B, C and D were lower in comparison to the control, while the proportion of arachidonic acid in groups B, C and D were significantly higher(p<0.01) in comparison to the control. 3. In order to determine the oxidative damages to the protein in MDBK cells, the patterns of the SDS-PAGE were examined and the patterns of SDS-PAGE in groups C and D were significantly different between 43kd and 200kd of molecular weight.

• Journal of the Korea Society of Computer and Information
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• v.29 no.2
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• pp.21-30
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• 2024

This research aims to design a system capable of generating real web pages based on deep learning and big data, in three stages. First, a classification system was established based on the industry type and functionality of e-commerce websites. Second, the types of components of web pages were systematically categorized. Third, the entire web page auto-generation system, applicable for deep learning, was designed. By re-engineering the deep learning model, which was trained with actual industrial data, to analyze and automatically generate existing websites, a directly usable solution for the field was proposed. This research is expected to contribute technically and policy-wise to the field of generative AI-based complete website creation and industrial sectors.

• Journal of KIISE:Information Networking
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• v.32 no.1
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• pp.110-122
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• 2005

Bluetooth has been reputed as a wireless networking technology supplying ad-hoc networks between digital devices. In particular, bluetooth scatternet is a most essential part for dynamic ad-hoc networks. But past researches on bluetooth scatternet has hardly treated dynamic scatternet environment. In this paper, we proposed a scatternet reformation algorithm for the case that some nodes escape from the scatternet. The proposed algorithm is a general algorithm which can be applied to many types of bluetooth scatternet regardless of the topology. The proposed algorithm has short reformation time delay because the process has only page process (not including inquiry process ). The algorithm is operated based on Recovery Node Vector which is composed of Recovery Master and Recovery Slave. In this paper, we performed the real hardware experiments for evaluating the performance of the proposed algorithm. In that experiments, we measured the reformation time and reformation probability. In comparison with the case including inquiry process, the proposed algorithm had the improvement in reformation time delay and we obtained high success rate over 97%.

• Journal of Plant Biology
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• v.36 no.4
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• pp.377-382
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• 1993

Rice (Oryza saliva L.) calli containing both embryogenic callus (EC) and non embryogenic callus (NEC) regions were initiated from the mature seed on MS medium supplemented with 2.0 mg/L 2,4-D, 0.5 mg/L kinetin. The calli were developed into two callus type which can be distinguished by visual examination depending on color and appearance. In order to illucidate the polypeptide composition between EC and NEC, the total protein extracted from two types of callus was analysed by electrophoresis. By one-dimesional anlaysis of SDS-PAGE and Isoelectric focusing, several protein bands showed quantitative and qualitative difference in each type of callus. The further analysis of the total protein with two-dimensional electrophoresis showed at least 20 EC specific protein and 10 NE specific protein. Also 3 specific protein spots showing micro heterogeneity of 90, 65, 50 kD were detected in EC, while a series of acidic heterologous protein spots were visualized in NEC.in NEC.

• Korean Journal of Fisheries and Aquatic Sciences
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• v.42 no.6
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• pp.574-579
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• 2009

Acid- and pepsin-solubilized collagens were extracted from the skin of shark (Isurus oxyrinchus) and their physicochemical properties were characterized by amino acid analysis, SDS-PAGE, the composition of collagen types, solubility and denaturation temperature. Acid - and pepsin-solubilized collagens from shark skin had an imino acid of 188.8 and 186.2 residues/1,000 amino acids, respectively. SDS-PAGE showed two different${\alpha}$ chains ($\alpha1$ and $\alpha2$) and $\beta$-component. The component ratio of type I and V was 10:1, and the type III was not found. Solubility of acid-soluble collagen was low in the range of pH 6.0 to pH 11.0. On the other hand, pepsin-solubilized collagen showed a low solubility in the range of pH 7.0-9.0. Temperature for denaturation of acid- and pepsin-solubilized collagens were $25^{\circ}C$ and $27^{\circ}C$, respectively.

• Proceedings of the Korea Contents Association Conference
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• 2005.11a
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• pp.269-274
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• 2005

There are lots of medicine information on the internet recently. But there is no specific research result about search patterns or acquisition methods of medicine information on web pages for lay people until now. In this paper, 1 analyzed the web log files of a certain company providing medicine information using the WiseLog tool. I analyzed three kinds of statistic result of the web log files such as the status of web page usage by types of users, the status of web page menu usage, and the status of search menu usage. As results, I proposed methods of supplement and improvement for companies providing medicine information on the internet.

• Journal of Plant Biology
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• v.38 no.4
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• pp.349-357
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• 1995

The alkaline invertase ($\beta$-D-fructofuranoside fructohydrolase, EC 3.2.1.26) was isolated and characterized from the hypocotyls of mung bean (Phaseolus radiatus L.). The enzyme was purified by consecutive step using diethylaminoethyl (DEAE)-cellulose anion exchange, 1st Sephadex G-200, DEAE-Sephadex A50 and 2nd Sephadex G-200 chromatography. The overall purification was about 77-fold with a yield of about 6%. The finally purified enzyme exhibited a specific activity of about 48 $\mu$mol of glucose produced mg-1 protein min-1 at pH 7.0 and appeared to be a single protein by nondenaturing polyacrylamide gel electrophoresis (PAGE). The enzyme had the native molecular weight of 450 kD and subunits molecular weight of 63 kD and 38 kD as estimated by Sephadex G-200 chromatography and SDS-PAGE, respectively, suggesting that the enzyme is a heteromultimeric protein composed of two types of subunits. On the other hand, the enzyme appeared to be not a glycoprotein according to the results of Con A chromatography and glycoprotein staining. The enzyme had a Km for sucrose of 19.7 mM at pH 7.0 and maximum activity around pH 7.5. The enzyme was most active with sucrose as substrate, compared to raffinose, cellobiose, maltose and lactose. These results indicate the alkaline invertase is a $\beta$-fructofuranosidase.

• Proceedings of the Korean Institute of Information and Commucation Sciences Conference
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• 2009.10a
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• pp.317-319
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• 2009

The physical disabled are using various types of assistive devices and reading assistance device is have been researched and development. After all reading assistance device which being marketed present for the physical disabled's features is simply limited to turing the page. In this paper, Implemented prototype model of the reading assistance device for the physical disabled that is have designed to modify to improve the success rate of turing page and conjunction with mobile devices such as PDA to provide more convenient and useful features. And not only for the disabled then take a look at Universal design concept of view of the tool can be useful the public also.

• Food Science of Animal Resources
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• v.42 no.1
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• pp.73-83
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• 2022

The aim of this study is to determine the effects of using emulsion manufactured with soybeans (ES) to substitute chicken breast in Vienna sausages. Four types of Vienna sausages (S1: 10% ES and 50% chicken, S2: 20% ES and 40% chicken, S3: 30% ES and 30% chicken, and S4: 40% ES and 20% chicken) for this study were made. The pH, color, proximate composition, sodium dodecyl sulfate-polyacrylamide gel electrophoresis (SDS-PAGE), microphotographs, cooking yields, and texture profile analysis of sausages were examined. The pH value of uncooked and cooked sausages increased significantly with increasing ES content (p<0.05). The crude protein contents of S2, S3, and S4 were significantly higher than that of the control (p<0.05). Furthermore, the SDS-PAGE results showed that α-conglycinin, β-conglycinin, and the acidic subunit of glycinin all increased with increasing ES content. Microphotographs revealed that increasing the ES content decreased the size of fat globules. The cooking yields of samples increased significantly with increasing ES content (p<0.05). The hardness values of ES treated samples were significantly lower than that of the control (p<0.05). Therefore, 30% substitute of chicken breast with ES can improve the quality and structure of Vienna sausage, without inducing critical defects.

• Journal of Information Technology Services
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• v.6 no.1
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• pp.17-30
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• 2007

The rapid development of information and communication technologies has resulted in rapid increase in the qualitative and quantitative information that can be accessed by information technology. Furthermore an enormous amount of information is provided in various formats. However, there are a group of people who are not able to even approach to targeted information due to physical or environmental problems. Visually handicapped users constitute a class of these users. In this paper, a method of developing user interface for web-based education service is described for the visually handicapped users to access information more efficiently. First, this paper analyzed the problems of visually handicapped users to access information targeting representative domestic sites that supply education services. And based on the results of the above analyses, Nest, this paper explains the web-based education service interface implementation method according to types of the content to be delivered. Lastly, the validity of this method is provided by testing a web page based on the implementation method according to the types of content.