• Reproductive and Developmental Biology
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• v.31 no.3
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• pp.161-167
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• 2007

In this study, we constructed and tested retrovirus vectors designed to express the human thrombopoietin gene under the control of the tetracycline-inducible promoters. To increase the hTPO gene expression at him-on state, WPRE sequence was also introduced into retrovirus vector at downstream region of either the hTPO gene or the sequence encoding reverse tetracycline-controlled transactivator (rtTA). Primary culture cells (PFF, porcine fetal fibroblast; CEF, chicken embryonic fibroblast) infected with the recombinant retrovirus were cultured in the medium supplemented with or without doxycycline for 48hr, and induction efficiency was measured by comparing the hTPO gene expression level using RT-PCR, western blot and ELISA. Higher hPTO expression and tighter expression control were observed from the vector in which the WPRE sequence was placed at downstream of the hTPO (in CEF) or rtTA(in PFF) gene. This resulting tetracycline inducible vector system may be helpful in solving serious physiological disturbance problems which have been a major obstacle in successful production of transgenic animals.

• Journal of Ocean Engineering and Technology
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• v.27 no.1
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• pp.85-92
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• 2013

This paper deals with the resonant type of a WEC (wave energy converter) and the determination method of its geometric parameters which were obtained to construct the robust and optimal structure, respectively. In detail, the optimization problem is formulated with the constraints composed of the response surfaces which stand for the resonance period(heave, pitch) and the meta center height of the buoy. Use of a signal-to-noise ratio calculated from normalized multi-objective results with the weight factor can help to select the robust design level. In order to get the sample data set, the motion responses of the power buoy were analyzed using the BEM (boundary element method)-based commercial code. Also, the optimization result is compared with a robust design for a feasibility study. Finally, the power efficiency of the WEC with the optimum design variables is estimated as the captured wave ratio resulting from absorbed power which mainly related to PTO (power take off) damping. It could be said that the resultant of the WEC design is the economical optimal design which satisfy the given constraints.

• Journal of Korean Society of Forest Science
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• v.97 no.1
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• pp.61-70
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• 2008

This study was conducted to develop a mobile tower-yarder with tractor for agriculture and forestry that is the efficient yarder in steep terrains, thinning operation and small scale logging operation. It was designed and manufactured that the power source of tower-yarder is equiped three hydraulic pump connected to PTO of tractor, and three hydraulic pump is used to operate the four motor for drum, the cylinder for clutch of interlocker, the cylinder for tower expanding and the out-rigger cylinder. It was to adopt the running skyline system and the inter-lock function, and to equip the double capstan drum, the storage drum and the clutch for interlock in the development of tower-yarder. It was to develop the tower-yarder which the winch torque of double-capstan drum, the traction force of double-capstan drum, the number of rotation of double-capstan drum and the line speed is $191kg{\cdot}m$, 1,910 kgf, 220.5 rpm and 138.5 m/min, respectively. And it was known that the optimum flange diameter of the main and haulback storage drum is about 360 mm and about 460 mm in order to storage the main line length of 250m and the haulback line length of 450 m. The carriage was made to adopt the running skyline system and to equip the lock function in order to the convenience of chocking and the fall down preventing of tree. It was provided to develop the wire remote controller for the inter-lock function, the convenience of control and the efficiency of yarding. In development process, this tower-yarder was attached the 3-point linkage hitch equipment and the tire wheel for the traction and moving of tower-yarder. Also, it was equipped that the out-rigger and the guy line in order to raise the safety and efficiency of yarding of tower-yarder.

• Journal of Biosystems Engineering
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• v.38 no.3
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• pp.171-179
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• 2013

Purpose: A simulator for the design and performance evaluation of a tractor with a hydro-mechanical transmission (HMT) was developed. Methods: The HMT consists of a hydro-static unit (HSU), a swash plate control system, and a planetary gear. It was modeled considering the input/output relationship of the torque and speed, and efficiency of HSU. Furthermore, a dynamic model of a tractor was developed considering the traction force, running resistance, and PTO (power take off) output power, and a tractor performance simulator was developed in the co-simulation environment of AMESim and MATLAB/Simulink. Results: The behaviors of the design parameters of the HMT tractor in the working and driving modes were investigated as follows; For the stepwise change of the drawbar load in the working mode, the tractor and engine speeds were maintained at the desired values by the engine torque and HSU stroke control. In the driving mode, the tractor followed the desired speed through the control of the engine torque and HSU stroke. In this case, the engine operated near the OOL (optimal operating line) for the minimum fuel consumption within the shift range of HMT. Conclusions: A simulator for the HMT tractor was developed. The simulations were conducted under two operation conditions. It was found that the tractor speed and the engine speed are maintained at the desired values through the control of the engine torque and the HSU stroke.

• Korean Journal of Agricultural Science
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• v.49 no.4
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• pp.845-855
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• 2022

The purpose of this study is to develop a self-propelled underground crop harvester and its performance was evaluated by measuring the load during actual potato harvesting operations. This study was conducted at a constant working speed of 1 km·h^-1. A load measurement system was installed to measure the actual load and the required working power was analyzed. A hydraulic pressure sensor was also installed to measure the hydraulic pressure. The required hydraulic power was calculated using the hydraulic pressure and flow rate. The results showed that the engine speed, torque, and power during harvesting operation were in the range of 845 - 1,423 rpm, 95 - 228 Nm, and 9 - 31 kW, respectively. Traction power, excluding the hydraulic pump of the tractor and power take-off (PTO) output, was in the range of 9 - 28 kW, and it was confirmed that it occupies a ratio of 16.2 to 50% of the engine rated output. The engine can supply the minimum required traction power to move the vehicle. This means that the engine used in this study could be down-sized to be suitable for an underground crop harvester. In this study, the gear stages of the tractor were not considered. This research thus shows the possibility of developing a self-propelled underground crop harvester.