• Fisheries and Aquatic Sciences
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• 제17권3호
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• pp.385-390
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• 2014

Abalone immortal cell lines can be used to study the physiological properties and disease mechanisms of abalone at the cellular and molecular level. As a first step for the final goal to establish abalone immortal cell lines, we examined various initial culture conditions for primary cell populations derived from Haliotis discus hannai radula tissue. The survival rate after cell isolation procedures using the enzymatic method was as low as $9.95{\pm}2.37%$. Based on three different experimental conditions for H. discus hannai radula-derived cell culture, we found that the salinity of the media and the presence of growth-promoting factors were important to support radula-derived primary cell populations during the initial culture. The growth factor-containing media adjusted to 35 psu salinity could induce 100% (8 out of 8 trials) initial cell attachment, and the rate of cell attachment reached 50-70%. The data obtained from this study will provide useful information for developing immortal cell lines from abalone species.

• Fisheries and Aquatic Sciences
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• 제13권4호
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• pp.343-349
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• 2010

The effects of osmotic stress on the non-specific immune response of Nile tilapia, Oreochromis niloticus, were investigated. Osmoregulatory mechanism of tilapia has been studied, but less information is available about innate immune response of O. niloticus faced with hyperosmolality. Acute osmotic stress was elicited by transferring tilapia from freshwater (FW) to 24 psu seawater (SW). Non-specific immune parameters including lysozyme activities of plasma and head kidney (HK), alternative complement pathway (ACP) activity in plasma, phagocytic capacities of spleen and HK immune cells, and respiratory burst activity of immune cells in both HK and spleen were analyzed. Lysozyme activities were increased at 1 h and 30 h after transfer to SW, but decreased at 10 h after SW transfer. Conversely, ACP activity increased 10 h after SW transfer. Phagocytic capacity increased slightly at 1 h and 5 h after SW transfer, and respiratory burst activity showed an increase in superoxide release at 10 h after SW transfer. Taken together, these results indicate that the exposure of tilapia to hyperosmotic conditions has immunostimulatory effects on cellular and humoral immune reactions.

• 한국환경독성학회:학술대회논문집
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• 한국환경독성학회 2003년도 추계국제학술대회
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• pp.158-158
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• 2003

Bioassays using gametes of sea urchins are widely used in ecotoxicological assessments of marine environments. Since most of sea urchin species in Korean coastal water spawn from spring to autumn, bioassay with them during the winter is impossible. In the course of developing standard methods for bioassays with Korean species, we found a winter-spawning starfish, Asterias amurensis, Since reproductive mode of asteroids is similar to echinoids, the bioassay protocol for sea urchins could be applied similarly to the starfish. Here, we tested and determined several conditions for the acceptability of bioassay with A. amurensis. The least required time for formation of fertilization membrane of fertilized eggs to be easily distinguished from unfertilized ones was 60 min. The threshold of sperm to egg ratio that could make acceptable fertilization rates in controls was 3000. The allowed time for manipulation of sperm after dilution in seawater was at most 3 hr. The optimal exposure time of sperms when the response against toxicant solution was relatively stable was in the range of 20-60 min. The tolerance range of sperms to the salinity of test solution was 26-38 psu. The sensitivity of A. amurensis sperm was intermediate among marine organisms commonly used in aquatic toxicity tests. The sperm bioassay with A. amurensis can be satisfactorily applied to toxicity assessments of marine environments.

• 한국패류학회지
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• 제20권1호
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• pp.45-53
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• 2004

This study have conducted to calibrate the productivities on the mussel (Mytilus galloprovincialis) cultures in Jinhae Bay. Annual water temperature, salinity and dissolved oxygen have varied to 7.2-$25.9^{\circ}C$, 32.9-34.0 psu, 7.9-8.7 mg/l, respectively. In April, the smallest size of spat, 3.3 mm in shell length, have recruited, and 82.5 mm have recorded to the maximum. In summer, variations of growing up between individuals showed both shell length and weights at same cohort. Annual production at 2 m, 5 m, 8 m depth calculated to 10.91, 10.85 and 9.19 gWWt/ind./yr, respectively. Mussel cultivated in the upper part of the water column (two meter depth) were significantly longer and heavier than those in lower part (eight meter depth). After the recruitment, monthly production showed gradual increment at all positions during summer. As results, annual production of mussel cultivated seemed to be controlled by conditions of growing up in summer. Based on the productivities calculated to this study, the total annual production of the mussel cultivated in Jinhae Bay have estimated about 196 ton/ha/yr.

• 수산해양교육연구
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• 제28권4호
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• pp.1068-1075
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• 2016

The suitable temperature and salinity for the long-distance transportation for the rockfish were investigated by assessing survival rate, metabolism, histological change. All experimental groups showed survival rates of 100%. Daily Oxygen consumption rhythm was decreased during nights and increased during days. Average oxygen consumption was significantly decreased as temperature and salinity were decreased. Ammonia excretion was significantly increased as temperature and salinity were decreased. Histological changes were observed in the skin and gill of the rockfish exposed to 10 psu under all the temperature conditions, with larger changes at $4^{\circ}C$. Further, nucleus deformation and uniformity in the cytoplasm were also observed.

• 한국환경과학회지
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• 제11권4호
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• pp.297-308
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• 2002

Field observations for research of oceanographic characteristics in waters adjacent to fish farm on land and water quality at area where water is supplied to fish farm and area where water is discharged from fish farm are carried out in coastal waters of Pyoson located in the southeastern region of Jeju Island in March, May, August and November of 2000. Semidiurnal tides to the northeast and southwest dominate. The mean velocities of current around neap tides and spring tides are 8.52cmㆍs$^{-1}$, 28.63cmㆍs$^{-1}$, respectively. The difference of temperature between area where water is supplied to fish farm and area where water is discharged from fish farm is about 0.6$^{\circ}C$. Seasonal variation range for temperature and salinity is 13.5$^{\circ}C$ and 3.5psu, individually. Concentrations of nitrate(NO$_3$$^{-}$), phosphate(PO$_4$$^{3-}$ ), silicate(SiO$_2$) and COD(chemical oxygen demand) are high in autumn. Particularly, concentrations of nitrate and COD in winter and spring at station where water is supplied to fish farm are higher than these at station where water is dischared from fish farm. However, concentration of phosphate and silicate at station where is supplied to fish farm in spring, summer and autumn is higher than that at station where water is dischared from fish farm. Water quality in the study area is grade 1 and this level is kept up in the whole year.

• 한국환경과학회지
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• 제19권6호
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• pp.703-714
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• 2010

In this study, we analyzed the impact of orographic and thermal forcing on the atmospheric flow field over the urban metropolitan areas on urban artificial buildings and future development plan. Several numerical experiments have been undertaken in order to clarify the impacts of the future development plan on urban area by analyzing practical urban ground conditions, we revealed that there were large differences in the meteorological differences in each case. The prognostic meteorological fields over complex areas of Seoul, Korea are generated by the PSU/NCAR mesoscale model(MM5). we carried out a comparative examination on the meteorological fields of topography and land-use that had building information and future development plan. A higher wind speed at daytimes tends to be forecasted when using new topography and land use data that have a high resolution with an appropriate limitation to the mixing height and the nocturnal boundary layer(NCB). During nighttime periods, since radiation cooling development is stronger after development plan, the decreased wind speed is often generated.

• 한국패류학회지
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• 제20권2호
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• pp.107-110
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• 2004

The filtration rate and oxygen consumption of Anadara granosa bisenensis were measured at 4 different temperature (10$^{\circ}C$, 15$^{\circ}C$, 20$^{\circ}C$, and 25$^{\circ}C$) and 4 different salinity levels (20, 25, 35, and 40 psu), using an direct method under laboratory conditions. The samples were divided into two groups: a large-sized group (shell length: 34.58 ${\pm}$ 2.23 mm, body weight: 12.43 ${\pm}$ 0.44 g) and a small-sized group (shell length: 20.60 ${\pm}$ 0.85 mm, body weight: 4.41 ${\pm}$ 2.30 g). Filtration rate and oxygen consumption increased with the increase of various temperatures and salinity levels. Also, the filtration rate and oxygen consumption of the small-sized group were higher than those of the large-sized group. These results indicate that the filtration rate and oxygen consumption of Anadara granosa bisenensis depends on environmental conditions, especially on water temperature and salinity.

• 전력전자학회:학술대회논문집
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• 전력전자학회 2008년도 추계학술대회 논문집
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• pp.29-32
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• 2008

최근 PDP Display 시장은 해를 거듭 할수록 가격 경쟁이 치열해 지고 있다. 그에 따라 PSU(Power Supply Unit) 모듈의 저 가격화 및 경량화가 요구되고 있고, 요구조건을 만족하기 위해 여러 출력을 하나로 통합화 하는 다 출력 구조의 변압기가 적용되고 있다. 본 논문에서는 서스테인 전원부($V_s$)와 어드레스 전원부($V_a$), 그리고 Multi 18V전원부($V_m$)를 하나의 변압기에 일체화 하였다. 그리고 어드레스 전원부($V_a$)와 Multi 18V전원부($V_m$)의 부하 추가에 따라 서스테인 전원부($V_s$)의 전압 이득 특성 및 공진특성 변화를 확인하였다. 또한 다 출력 LLC 직렬공진 컨버터 설계 시 고려사항과 동작 특성으로 42인치 HD PDP 전원에 적용하여 검증한 결과에 대하여 서술 하였다.

• 대한원격탐사학회지
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• 제20권3호
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• pp.153-162
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• 2004

In the summer of 1998-2001, a huge flood occurred in the Yangtze River in the eastern China. Low salinity water less than 28 psu from the river was detected around the southwestern part of the Jeju Island, which is located in the southern part of the Korean Peninsula. We studied how to detect low salinity water from the Yangtze River, that cause a terrible damage to the Korean fisheries. We established a relationships between low salinity at surface, turbid water from the Yangtze River and digital ocean color remotely sensed data of SeaWiFS sensor in the northern East China Sea, in the summer of 1998, 1999, 2000 and 2001. The salinity charts of the northern East China Sea were created by regeneration of the satellite ocean color data using the empirical formula from the relationships between in situ low salinity, in situ measured turbid water with transparency and SeaWiFS ocean color data (normalized water leaving radiance of 490 nm/555 nm).