• Title/Summary/Keyword: PSP

Search Result 296, Processing Time 0.03 seconds

Competitive Spectrophotometry for Microbial Dipeptide Transport Systems

  • Hwang, Se-Young;Ki, Mi-Ran;Cho, Suk-Young;Lim, Wang-Jin;Yoo, Ick-Dong
    • Journal of Microbiology and Biotechnology
    • /
    • v.6 no.2
    • /
    • pp.92-97
    • /
    • 1996
  • Portage kinetic constants of peptide transport can be measured by competitive spectrophotometry. The kinetic constants of L-Glu-L-Glu transport in Escherichia coli were ascertained using L-Phe-L-3-thia-Phe (PSP) as a detector. Since the production of thiophenol upon intracellular hydrolysis of PSP was competitively inhibited by L-Glu-L-Glu, it was able to compute the kinetic constants of L-Glu-L-Glu using this method. The resulted data were in agreement with the values obtained by the method of Michaelis-Menten kinetics. The potential of this method was examined against dipeptide transport systems in various microorganisms. These results strongly suggest that the overall properties of individual systems for dipeptide transports can be easily characterized by competitive spectrophotometry.

  • PDF

Practical Use of Tissue Biosensor for Safety Test of Marine Organism and Monitoring of Public Health and Environment (해양 유독생물의 독성 검사와 보건환경 모니터링을 위한 조건센서의 활용)

  • 천병수;유종수;유진형;도변탈생
    • KSBB Journal
    • /
    • v.14 no.1
    • /
    • pp.1-8
    • /
    • 1999
  • It confirmed the facilitated diffusion of $Na^+$ of frog bladder membrane which is a tissue membrane. The mechanism was explained in $Na^+$ channel model and its referred to the $Na^+$ channel obstruction ingredient which was contained in the reference to the $Na^+$ channel obstruction ingredient and son on, e.g., seaweed, shellfish, pufferfish, phytoplankton and chinese drug. Also, it introduces the result which studied from the barrier point of the application of the tissue biosensor to the trade friction on Korea or Japan pufferfish and the marine environment in the one with high dependance. It was possible for the poison quantity of small amount pufferfish toxin (TTX), paralytic shellfish poisoning (PSP) to be measured and also to measure poison quantity in the cultivation poisonous toxin phytoplankton individual. In future, as for this tissue biosensor, it expects that it is possible to contribute widely until environment watch and also monitoring to the scene.

  • PDF

A Study on the Reliability Evaluation of Power Distribution System with Distributed Generations using Power Supplied Probability (전력공급확률을 이용한 분산형 전원을 고려한 배전계통 신뢰도 평가에 관한 연구)

  • Lee, Hee-Tae;Moon, Jong-Fil;Kim, Jae-Chul
    • The Transactions of The Korean Institute of Electrical Engineers
    • /
    • v.59 no.12
    • /
    • pp.2119-2124
    • /
    • 2010
  • Reliability evaluation of power distribution system is the evaluation for all customers supplied from one power source as main transformer located in substation. However, power sources include not only the main transformer but distributed generations. Typical reliability evaluation has focused on configuration of power system with one source including failure rates of equipment. In this paper, we focus on not only configuration but power sources as distributed generations. New reliability evaluation method using power supplied probability (PSP) is proposed. The proposed evaluation method are proved through case studies.

On the Some Toxic Dinoflagellates (유독플랑크톤(과편모조류를 중심으로)에 관한 고찰)

  • HAN Myung-Soo
    • Korean Journal of Fisheries and Aquatic Sciences
    • /
    • v.23 no.1
    • /
    • pp.51-60
    • /
    • 1990
  • Some species of dinoflagellates were considered as one of the causative organisms of PSP(Paralytic Shellfish Poison) or DSP(Diarrhetic Shellfish Poison). Fish and shellfish are intoxicated by feeding of toxic plankton, sometimes human is intoxicated by feeding on these intoxicated fish and shellfish. In past ten years, the physiological and ecological studies of the toxic plankton has been investigated for development of monitoring system and preventation and control measures of PSP. However, in our country still little is known on a research for the toxic dinoflagellates. This paper reviews the general biology, taxonomic problem, physioecology and culture method of the toxic planktons such as Protogony-aulax and Dinophysis.

  • PDF

Inositol(1,4,5)triphosphate 3-Kinase의 유전자 재조합과 CCL39 Hamster Lung Fibroblasts에서 발현

  • 김재웅;최관용
    • The Korean Journal of Food And Nutrition
    • /
    • v.9 no.2
    • /
    • pp.123-136
    • /
    • 1996
  • IPSKCDNA gene(1.8 kbp) encoding rat brain IP3K enzyme contained Not I restric site in open reading frame. The Not I sequence, GCGGCCGC, was converted to GCAGCCGC by site-directed mutagenesis. The mutated IP3KcDNA was digested with EcoR I and ligated with EcoR I-restricted psp72·Not2 vector. The resulting psp72 · Not2-IP3KCDNA was digested with the Not I restriction enzyme and then subcloned into the Not I -digested PZIP · NeoSV(X) mammalian expression vector. The PZIP · NeoSV(X) -IPSKCDNA was transfected into CCL39 hamster lung fibroblast cells. The efficiency of the expressed IPSKCDNA gene was significantly higher than expected generally, not only a mean 5-fold increase in the amount of enzyme, but also 16-fold increase in enzyme activity from tractsfected CCL39 cells by the method of Western blot using anti-lP3K antibodies. Both distribution of IPSK in various rat tissues and biochemical properties were discussed.

  • PDF

A Study on the Activities and Decision Factors between In-house vs. Outsourcing Reverse Logistics Processes (역물류(Reverse Logistics)의 아웃소싱을 위한 연구)

  • Kim, Young-Ah
    • Proceedings of the Korean DIstribution Association Conference
    • /
    • 2006.08a
    • /
    • pp.119-131
    • /
    • 2006
  • Reverse logistics (RL) covering the flow of products/parts in a reverse direction from product returns to product disposal. Companies are faced with a decision on whether to manage RL in house or to outsource it to a RL third party service provider (3PSP). A survey was conducted in 2004 to study RL outsourcing practices. Through the survey, various RL functions outsourced in industry are identified, and the factors considered to decide between in-house vs. outsourcing RL management are studied along with their importance. In addition, this research presents the performance measures of a 3PSP and problems faced by companies while managing RL in-house and outsourcing.

  • PDF

Pneumothorax

  • Choi, Won-Il
    • Tuberculosis and Respiratory Diseases
    • /
    • v.76 no.3
    • /
    • pp.99-104
    • /
    • 2014
  • Pneumothorax-either spontaneous or iatrogenic-is commonly encountered in pulmonary medicine. While secondary pneumothorax is caused by an underlying pulmonary disease, the spontaneous type occurs in healthy individuals without obvious cause. The British Thoracic Society (BTS, 2010) and the American College of Chest Physicians (ACCP, 2001) published the guidelines for pneumothorax management. This review compares the diagnostic and management recommendations between the two societies. Patients diagnosed with primary spontaneous pneumothorax (PSP) may be observed without intervention if the pneumothorax is small and there are no symptoms. Oxygen therapy is only discussed in the BTS guidelines. If intervention is needed, BTS recommends a simple aspiration in all spontaneous and some secondary pneumothorax cases, whereas ACCP suggests a chest tube insertion rather than a simple aspiration. BTS and ACCP both recommend surgery for patients with a recurrent pneumothorax and persistent air leak. For patients who decline surgery or are poor surgical candidates, pleurodesis is an alternative recommended by both BTS and ACCP guidelines. Treatment strategies of iatrogenic pneumothorax are very similar to PSP. However, recurrence is not a consideration in iatrogenic pneumothorax.

Sequential Induction of Ethylene, Lipoxygenase, and Ascorbate Peroxidase in Senescing Soybean Callus

  • Ha, Mi-Young;Kim, Do-Kyun;Kim, Soon-Young;Kang, Bin-G.;Oh, Seung-Eun
    • BMB Reports
    • /
    • v.29 no.6
    • /
    • pp.564-568
    • /
    • 1996
  • Bursts of ethylene production occurred in twice at an early exponential (EEP) and prestationary (PSP) phases, respectively, during growth of callus tissue isolated from the root of soybean seedlings. The second burst of ethylene production at PSP was smaller in magnitude than the earlier one at EEP, but was followed by increases in both guaiacol peroxidase (GuPOX) and ascorbate peroxidase (AsPOX). The increase in AsPOX activity was also preceded by an increase in lipoxygenase (LOX) activity. Treatment of the tissue with the ethylene antagonist 2,5-norbonadiene (NBD) resulted in substantial reduction in LOX and AsPOX activities during this period. GuPOX activity was reduced only slightly, if any, by NBD. Role of ethylene in the sequential induction of LOX and AsPOX in senescing callus tissue is discussed.

  • PDF

Effect of Mitomycin C on Chinese Hamster Cells (培養한 Chinese Hamster 細胞에 미치는 Mitomycin C 의 影響)

  • Hahn, Sahsook
    • The Korean Journal of Zoology
    • /
    • v.16 no.4
    • /
    • pp.211-217
    • /
    • 1973
  • Mitomycin C, chemically reactive antibiotics derived from Streptomyces caespitosus, was introduced to Chinese hamster, Cricetulus griseus, (2n=22) cells (22Emb. ♀5PSP ♂, to carry out an analysis of the chromosome aberration. It was found that regions 5 and 7 of chromosomes 1 and 2, and secondary constriction of chromosome X showed the most striking effect of Mitomycin C. The relationship between Mitomycin C and secondary contriction was discussed.

  • PDF