• Asian-Australasian Journal of Animal Sciences
• /
• v.17 no.7
• /
• pp.897-902
• /
• 2004

PSMC5 subunit, which belongs to the 26S proteasomal subunit family, plays an important role in the antigen presentation mediated by MHC class I molecular. Full-length cDNA of porcine PSMC5 was isolated using the in silico cloning and rapid amplification of cDNA ends (RACE). Amino acid was deduced and the primary structure was analyzed. Results revealed that the porcine PSMC5 gene shares the high degree of sequence similarity with its mammalian counterparts at both the nucleotide level and the amino acid level. The RT-PCR was performed to detect the porcine PSMC5 expression pattern in seven tissues and the result showed that high express level was observed in spleen, lung, marrow and liver while the low express level was in muscle. The full-length genomic DNA sequence of porcine PSMC5 gene was amplified by PCR and the genomic structure revealed that this gene was comprised by 12 exons and 11 introns. Best alignment of the cDNA and genomic exon DNA sequence presents 4 mismatches and this information potentially bears further study in gene polymorphisms.

• Food Science and Preservation
• /
• v.16 no.3
• /
• pp.419-426
• /
• 2009

The antioxidant and antimicrobial effects of acid-soluble and pepsin-solubilizable shark (Isurus oxyrinchus) collagens (SC) (ASSC: acid-soluble shark skin collagen, ASMC: acid-soluble shark meat collagen, PSSC: pepsin-solubilizable shark skin collagen, PSMC: pepsin-solubilizable shark meat collagen) and standard marine collagen (STMC) as materials, and the ability of these materials to inhibit tyrosinase and elastase, were investigated. The electron-donating ability of SC ($1{\sim}5\;g/mL$) was $14.91{\sim}17.21%$, which was $3.0{\sim}3.6$-fold higher than that of STMC at the same concentration. Also, the SOD(superoxide dismutase)-like activity of SC (5.80 mg/mL) was $4.67{\sim}37.28%$, thus $3.0{\sim}3.6$-fold greater than that of STMC. The MIC values of SC against Staphylococcus aureus and Salmonella enteritidis were $5{\mu}g$/disc, which were remarkably lower than that of STMC ($200{\mu}g$/disc). There was no antimicrobial activity against Escherichia coli in STMC, but the MIC against E. coli was $200{\mu}g$/disc for acid-soluble SC and $100{\mu}g$/disc for pepsin-solubilizable SC. The inhibition of tyrosinase by SC (3-5 mg/mL) was $58.95{\sim}98.16%$, $3.34{\sim}3.74$-fold higher than that of STMC ($17.67{\sim}26.25%$). Also, elastase inhibition by SC (at 1 mg/mL) was $53.33{\sim}80.0%$, $1.1{\sim}4.0$-fold greater than that of STMC. These results indicated that shark collagens may be valuable new functional materials owing to their antioxidant and antimicrobial properties, and because the inhibitory activities against elastase and tyrosinase are better than those of standard marine collagen.

• BMB Reports
• /
• v.44 no.9
• /
• pp.578-583
• /
• 2011

Aspartate aminotransferase (AST) and alanine aminotransferase (ALT) are biochemical markers used to test for liver diseases. Copy number variation (CNV) plays an important role in determining complex traits and is an emerging area in the study various diseases. We performed a genome-wide association study with liver function biomarkers AST and ALT in 407 unrelated Koreans. We assayed the genome-wide variations on an Affymetrix Genome-Wide 6.0 array, and CNVs were analyzed using HelixTree. Using single linear regression, 32 and 42 CNVs showed significance for AST and ALT, respectively (P value < 0.05). We compared CNV-based genes between the current study (KARE2; AST-140, ALT-172) and KARE1 (AST-1885, ALT-773) using NetBox. Results showed 9 genes (CIDEB, DFFA, PSMA3, PSMC5, PSMC6, PSMD12, PSMF1, SDC4, and SIAH1) were overlapped for AST, but no overlapped genes were found for ALT. Functional gene annotation analysis shown the proteasome pathway, Wnt signaling pathway, programmed cell death, and protein binding.

• The Bulletin of The Korean Astronomical Society
• /
• v.36 no.2
• /
• pp.119.2-119.2
• /
• 2011

대 소 마젤란은하는 항성진화 및 은하진화를 연구할 수 있는 가장 중요한 실험실이다. 두 은하는 우리은하에 매우 가깝고, 각크기가 매우 크기 때문에 이들 두 은하에 대한 측광학적 연구는 매우 지엽적이고 단편적으로 이루어졌다. 시상이 매우 좋은 KMTNet의 1호기 (칠레)를 사용하여 대마젤란은하 ($10^{\circ}{\times}10^{\circ}$)와 소마젤란은하 ($5^{\circ}{\times}5^{\circ}$) 영역에 대한 UBVI 및 협대역 $H{\alpha}$, [OIII] $5007{\AA}$ 측광 탐사관측을 통해 다음과 같은 연구를 제안한다. 1. $V{\approx}23$ 등급까지 대 소 마젤란은하 천체들의 측광 자료의 제공 2. 거리를 지시할 수 있는 천체들의 공간적 분포를 통해 3차원 구조 연구 3. 협대역 $H{\alpha}$ 측광을 통해 Herbig Ae/Be 천체들의 분포와 별 탄생 연구 4. 협대역 $H{\alpha}$ 및 O[III] 측광을 통해 행성상성운의 분포와 광도함수 연구 5. 색-등급도 연구를 통해 위치에 따른 별 탄생의 역사 연구 6. 어두운 별들의 공간적 분포를 통해 LMC-SMC, LMC-MWG, SMC-MWG의 상호작용 연구.