• Proceedings of the PSK Conference
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• 2003.10b
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• pp.118.3-119
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• 2003

Diazepam (DZ) is one of the most frequently prescribed drugs as an antianxiety agent, muscle relaxant, and anticovulsant and sometimes causes intoxication due to accidental overdose, misuse or abuse. Screening or confirmation methods for DZ and NDZ in plasma are very important for clinical and toxicological studies and in forensic cases. GC/MS assay with SPE was developed for the determination of diazepam and its metabolite, nordiazepam in human plasma. Diazepam in plasma was extracted by a rapid and sensitive procedure based on C18 bonded-phase extraction. (omitted)

• Proceedings of the PSK Conference
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• 2003.10b
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• pp.125.3-126
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• 2003

Effect of Compound-A, a phenylpropanoid isolated from Arctium lappa fruit, on heterologous passive cutaneous anaphylaxis (HPCA), the release of histamine, and Phospholipase $A_2$ (PLA2) and phosphadiesterase (PDE) activities were studied by the method of Levine and Vaz. Anti-serum was prepared from ovalbumin (OA)-sensitized male Balb/c mouse at two weeks after the last challenge of OA and alumina gel. Heterologous PCA test in rats were carried out to determine the contents of leakaged pigment in the dorsal skin 30 minutes after i.v. injection of 0.2 ml of 1 % OA and 1 % Evans blue mixture (1:1). (omitted)

• Proceedings of the PSK Conference
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• 2003.10b
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• pp.157.2-157.2
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• 2003

Panax ginseng is one of the most important medicinal plant in the Orient. The international trade of ginseng is increasing yearly. The disguise of Chinese and American ginseng into Korean ginseng became a problem in recent years in Korea and an abroad. Obviously, an effective method of authentication of Korean ginseng from others at a DNA level, is necessary for the healthy development of the ginseng market. In order to develop convenient and reproducible methods for the identification of Korean ginseng, amplified fragment length polymorphism (AFLP) analysis was applied within Panax species (Korean cultivatied and wild ginseng, Chinese wild ginseng, American cultivatied and wild ginseng). (omitted)

• Proceedings of the PSK Conference
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• 2003.10b
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• pp.170.3-171
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• 2003

An efficient Erythropoietin (EPO)-expression system in mammalian cells is required for massive production for therapeutic use. Ammonium ion is a major problem in the production of valuable recombinant proteins in cultured animal cells. Therefore, it is of importance to devise a system by which a high productivity of human therapeutic recombinant protein can be maintained or enhanced under low ammonium concentration. To reduce the ammonium ion accumulated in EPO producing Chinese Hamster Ovary (CHO) ceels, IBE, we introduced the first two genes of the urea cycle, carbamoyl phosphate synthetase (CPSI) and arnithine transcarbamoylase (OTC), into IBE using a stable transfection method. (omitted)

• Proceedings of the PSK Conference
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• 2003.10b
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• pp.185.2-185.2
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• 2003

The typical approach of virtual screening is to prepare a 3D database and dock each member to the receptor, and carry out a post-analysis to make a final selection of compounds to be tested. The biological test of these compounds leads to 'hit'. The size of the 3D database is rate-determining factor because the docking process is still time-consuming method. The number of compounds for biological testing is cost-determining factor because the materials used in the test are cost-consuming. The use of the representative subsets derived from the entire database help reduce runtime of docking procedure. (omitted)

• Proceedings of the PSK Conference
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• 2003.10b
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• pp.200.1-200.1
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• 2003

Cephalotaxus koreana Nakai is an endemic species in Korea. The EtOH extract of leaf and branch from the plant showed potent antitumor activity in Teruhiro's method. The tumor volume inhibition ratio value is 25.2% with 20mg/kg in the BDF1 mouse injected LLC cell. We isolated one flavone, sciadopitysin (1), two flavone O-glycosides, quercetin 3-O-${\beta}$-D-glucuronide 6"-ethyl ester (2), apigenin 7-neohesperidoside (3) in comparison with literatures data. Compounds 1-3 showed stronger antitumor activity than Taxol used as positive control. The inhibition ratio values of compounds 1-3 is 34.9, 31.6, 34.0%, respectively, and Taxol is 27.0 % compared with control group.

• Proceedings of the PSK Conference
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• 2003.10b
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• pp.210.3-210.3
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• 2003

This study was performed to determine the cytotoxic effect of methanol extract from medicinal plants. The cell viability was determined by the MTT method. Their cytotoxic activities against three cancer cell lines such as A549, MDA-MB-231 and SNU-C4 cell line were tested. Among them, The methanol extract of Saururus Chinensis Bail showed the strongest cytotoxic effect against SNU-C4 cells. These results suggest that the methanol extract of Saururus Chinensis Bail possessed a potential antitumorous agent

• Proceedings of the PSK Conference
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• 2003.10b
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• pp.205.3-206
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• 2003

The genus Styrax (Styracaceae) is different from other genera of this family due to the production of resinous material, usually secreted when the barks and trunks are injured by sharp objects. This resin, in the past considered a miraculous remedy in several parts of Asia and America, has been used in traditional medicine to treat inflammatory diseases. The CH$_2$Cl$_2$ fraction of Styrax japonica showed significant cytotoxic activities by SRB method against five human tumor cell lines (A549, HCT-15, MES-SA, SK-OV-3, and SK-MEL-2). (omitted)

• Proceedings of the PSK Conference
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• 2003.10b
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• pp.207.1-207.1
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• 2003

The Aster tataricus is a chinese traditional medicine called “Ziwan” which has an expectorative and remediable cough action. The anti-oxidant activities of A. tataricus were investigated. The MeOH extract of A. tataricus showed strong anti-oxidant activity in the NBT(nitroblue tetrazolium) method system, and thus fractionated with several solvents in to the EtOAc, n-BuOH, CH$_2$Cl$_2$, H$_2$O fraction. The EtOAc soluble fraction exhibiting strong anti-oxidant activity was further purified by repeated silica gel and sephadex LH-20 column chomatography. (omitted)

• Proceedings of the PSK Conference
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• 2003.10b
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• pp.203.1-203.1
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• 2003

Cudrania tricuspidata have been used for anti-inflammatory, anti-hepatotoxic, anti-hypertensive and anti-diabetic activities. In this study, in order to investigate the efficacy of antioxidant activity, the bio-activity guided fraction and isolation of physiologically active substance were performed. H$_2$O, 30%, 60%, 100% MeOH and acetone fractions were examined antioxidant activity by DPPH method. It was revealed that 30%, 60%, 100% MeOH frations have significantly antioxidant activity. (omitted)