• Molecules and Cells
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• v.46 no.4
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• pp.219-230
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• 2023

Down syndrome (DS) is the most common autosomal aneuploidy caused by trisomy of chromosome 21. Previous studies demonstrated that DS affected mitochondrial functions, which may be associated with the abnormal development of the nervous system in patients with DS. Runt-related transcription factor 1 (RUNX1) is an encoding gene located on chromosome 21. It has been reported that RUNX1 may affect cell apoptosis via the mitochondrial pathway. The present study investigated whether RUNX1 plays a critical role in mitochondrial dysfunction in DS and explored the mechanism by which RUNX1 affects mitochondrial functions. Expression of RUNX1 was detected in induced pluripotent stem cells of patients with DS (DS-iPSCs) and normal iPSCs (N-iPSCs), and the mitochondrial functions were investigated in the current study. Subsequently, RUNX1 was overexpressed in N-iPSCs and inhibited in DS-iPSCs. The mitochondrial functions were investigated thoroughly, including reactive oxygen species levels, mitochondrial membrane potential, ATP content, and lysosomal activity. Finally, RNA-sequencing was used to explore the global expression pattern. It was observed that the expression levels of RUNX1 in DS-iPSCs were significantly higher than those in normal controls. Impaired mitochondrial functions were observed in DS-iPSCs. Of note, overexpression of RUNX1 in N-iPSCs resulted in mitochondrial dysfunction, while inhibition of RUNX1 expression could improve the mitochondrial function in DS-iPSCs. Global gene expression analysis indicated that overexpression of RUNX1 may promote the induction of apoptosis in DS-iPSCs by activating the PI3K/Akt signaling pathway. The present findings indicate that abnormal expression of RUNX1 may play a critical role in mitochondrial dysfunction in DS-iPSCs.

• KSCE Journal of Civil and Environmental Engineering Research
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• v.30 no.2A
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• pp.137-148
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• 2010

This study proposes the optimal ratio of vertical load-carrying capacity (${\beta}$) by investigating structural performances and economic efficiency in the extradosed bridges. Without design standards for the extradosed bridge, Japanese design standards have been used domestically. For the design live load, DL24 is found to be more adequate than DB24. Using the DL24 load, parameter studies are carried out. The parameters are 'main tower height', 'main girder stiffness', and 'cable arrangement'. As a result, it is found that one side cable-stayed extradosed bridges are more economical than double side cable-stayed extradosed bridges. This study also shows that when the ratio of vertical load-carrying capacity(${\beta}$) is 30~50% in the extradosed bridge with the ratio of tower height to main span length 1/6, the extradosed bridge is most economical because of the cable stress less than the allowable stress.

• KSCE Journal of Civil and Environmental Engineering Research
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• v.29 no.2A
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• pp.131-143
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• 2009

Under transverse earthquake shaking, arching action of bridge structures develops along the deck between the abutments thus providing the so-called deck resistance. The magnitude of the arching action for bridge structures is dependent on the number of spans, connection condition between deck and abutment or piers, and stiffness ratio between superstructure and substructure. In order to investigate the arching action effects for inelastic seismic responses of PSC Box bridges, seismic responses evaluated by pushover analysis, capacity spectrum analysis and nonlinear time-history analysis are compared for 18 example bridge structures with two types of span numbers (short bridge, SB and long bridge, LB), three types of pier height arrangement (regular, semi-regular and irregular) and three types of connection condition between superstructure and substructure (Type A, B, C). The arching action effects (reducing inelastic displacement and increasing resistance capacity) for short bridge (SB) is more significant than those for long bridge (LB). Semi-regular and irregular bridge structures have more significant arching action than regular bridges.

• KSCE Journal of Civil and Environmental Engineering Research
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• v.30 no.3A
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• pp.287-295
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• 2010

The main purpose of this study is to investigate the static behavior of spliced prestressed concrete girder with bending moment connector and lateral prestressing. Four (4) spliced girders and one (1) monolithic girder had been fabricated and tested to compare their static behaviors. Same geometry and materials are used to fabricate these spliced and monolithic girders. A monolithic girder and one (1) spliced girder without lateral bending connector are used as control specimens to estimate the performance of three (3) spliced girders with lateral bending connector. Deflections at the middle of girders have been measured for evaluation. Also, strains of the concrete at the middle of span and connection points have been measured. It was found from the result that laterally strengthened spliced girders showed improved ultimate strength but less stiffness compared to the monolithic girder at the ultimate state. Laterally strengthened spliced girder also showed improved strength as well as improved stiffness compared to the spliced girder without lateral strengthening.

• Journal of the Korea institute for structural maintenance and inspection
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• v.28 no.1
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• pp.61-69
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• 2024

An ultra-high strength prestressed prismatic beam of 100 MPa in compressive strength was developed by increasing the water-tightness of concrete by utilizing centrifugal molding processes without adding expensive admixtures. The centrifugal prismatic PSC beam developed as the superstructure of the avalanche tunnel was constructed on a rahmen bridge in a small local river. In this study, the centrifugal prismatic beam was compared and analyzed based on the results of measurements made through static load tests and the results of numerical analysis of the target structure. The common load-carrying capacity and safety of the rahmen bridge were evaluated. The static·dynamic load tests and finite element analysis results of this bridge were similar, and it was confirmed that the behavior of the centrifugal prismatic beam was well simulated. All centrifugally formed square beams that make up the composite rahmen bridge were evaluated to secure sufficient load carrying capacity under the design live load, and structural reliability was proven by ensuring safety.

• Reproductive and Developmental Biology
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• v.39 no.3
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• pp.89-95
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• 2015

The addition of growth factors and cytokines to in vitro culture (IVC) media could affect embryo development and the quality of the resulting blastocysts. The present study was performed to investigate the effect of porcine induced pluripotent stem cell (piPSC)-culture conditioned medium (CM) on the in vitro maturation (IVM) and development of parthenogentic embryos (parthenotes) in pigs. Cumulus-oocyte complexes (COCs) or activated oocytes were cultured in IVM or IVC medium supplemented with 0 (control), 25, or 50% of stem cell medium (SM) or CM, respectively. The maturation rate of CM-25% group was significantly improved when compared with control group (p<0.05), but that was not different among SM or CM groups. Blastocyst formation rate was significantly higher in CM-25% group (29.2%) than that of control (20.7%), SM-50% (19.6%) and CM-50% (23.66%, p<0.05). Cell number and the apoptotic cell index in blastocysts was significantly lower in SM-25% than in CM-25% group (p<0.05). The embryo quality related genes, OCT4, KLF4, TERT and ZFP42, were significantly increased in CM-25% group compared with control (p<0.05). In conclusion, the addition of 25% of CM to IVM and IVC medium positively influences not only the developmental potential also quality of parthenotes in pig.

• Journal of Korean Society of Steel Construction
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• v.16 no.6 s.73
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• pp.725-736
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• 2004

A new type of girder, called steel-confined prestressed concrete girder (SCP girder), has been developed, which maximizes the structural advantages of concrete, steel, and PS tendon, and improves on the shortcomings of steel plate girder, PSC I-girder, and preflex girder bridge for use in the construction of middle- or long-span bridges. To verify the propriety of design, structural safety, and applicability of this girder, a static load test was carried out (Kim et al.., 2002). Since the main damage typically sustained by steel bridges results from the fatigue caused by the repetition of traffic loads, fatigue safety must therefore be guaranteed in applying the SCP girder in the construction of real bridges. In this study, a fatigue test was carried out to investigate fatigue behavior and provide basic data for fatigue design. Based on the fatigue test, the fatigue safety of the girder was estimated. For the fatigue test, 10-m specimens were designed for a standard-design truckload (DB-24). A static load test was also performed before the fatigue test to analyze the structural behavior of the specimens. After the fatigue test, outer steel plates were removed to observe the condition of the concrete in the girder.

• Korean journal of food and cookery science
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• v.12 no.4
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• pp.499-505
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• 1996

The facillities for Kimchi preservation were investigated in order to develop the taste, flavor and commercial quality of Kimchi during fermentation. Four kinds of facillities used for this experiment were PP(Polypropylene). PSC(Polypropylene+ceramic)and PPP was selected newly and laminated of three layers with PETG (Polyester G), PS (Polystyrene) and PETG. The change of total number of lactic acid Bacteria, pH, acidity, color, gas contents and free internal volume of package were measured for the Kimchi packaged by 4 facillities during 6 days fermentation at 15$^{\circ}C$. The total No. of lactic acid Bacteria within the Kimchi in the PPP facillity was more and remained longer time (120 hrs.) than other Kimchi in the PP, PS and PPC facillities. Also the pH of all Kimchi were decreased to pH 4 within 72 hrs. and the pH of Kimchi in the PPP facillity was kept as pH 4 for 120 hrs., so that was shown to be decreased more dully than others. In case of fimchi in the PPP facillity, the color was retained better, CO$_2$ contents was lower similar to PPC facillity, and swelling degree of free internal volume was higher than others. By the sensory evaluation, the Kimchi in the PPP facillity represented as better than others for color, flavor, texture and total preferences until 48 hrs. fermentation. And the PPP facillity is transparent, so these will be selected and confirmed more easily, also PPP facillity is so hard to endure the swelling pressure of internal gas. Therefore it is thought that the PPP facillity used as Kimchi package will be desirable for better taste, flavor, and commercial quality.

• Bulletin of the Korean Chemical Society
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• v.33 no.2
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• pp.629-635
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• 2012

Two novel conjugated copolymers utilizing 4,7-dithien-2-yl-2,1,3-benzothiadiazole (DTBT) coupled with cyano (-CN) substituted vinylene, as the electron deficient moeity, have been synthesized and evaluated in bulk heterojunction solar cell. The electron deficient moeity was coupled with carbazole and fluorene unit by Knoevenagel condition to provide poly(bis-2,7-((Z)-1-cyano-2-(5-(7-(2-thienyl)-2,1,3-benzothiadiazol-4-yl)-2-thienyl)ethenyl)-alt-9-(1-octylnonyl)-9H-carbazol-2-yl-2-butenenitrile) (PCVCNDTBT) and poly(bis-2,7-((Z)-1-cyano-2-(5-(7-(2-thienyl)-2,1,3-benzothiadiazol-4-yl)-2-thienyl)ethenyl)-alt-9,9-dihexyl-9H-fluoren-2-yl) (PFVCNDTBT). The optical band gaps of PCVCNDTBT (1.74 eV) and PFVCNDTBT (1.80 eV) are lower than those of PCDTBT (1.88 eV) and PFVDTBT (2.13 eV), which is advantageous to provide better coverage of the solar spectrum in the longer wavelength region. The high $V_{oc}$ value of the PSC of PCVCNDTBT (~0.91 V) is attributed to its lower HOMO energy level ( 5.6 eV) as compared to PCDTBT ( 5.5 eV). Bulk heterojunction solar cells based on the blends of the polymers with [6,6]phenyl-$C_{61}$-butyric acid methyl ester ($PC_{61}BM$) gave power conversion efficiencies of 0.76% for PCVCNDTBT under AM 1.5, 100 mW/$cm^2$.

• Journal of the Korea Concrete Institute
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• v.26 no.1
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• pp.3-12
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• 2014

Hybrid Truss Bridge (HTB) uses steel truss webs instead of concrete webs in prestressed box girder bridges, which is becoming popular due to its structural benefits such as relatively light self-weight and good aesthetics appearance. Since the core technology of this bridge is the connection system between concrete slabs and steel truss members, several connection systems were proposed and experimentally evaluated. Also, the selected joint system was applied to the real bride design and construction. The research was performed on the connection system, since it can affect the global behavior of this bridge such as flexural and fatigue behaviors as well as the local behavior around the connection region. The evaluation study showed that HTB applied to a curved bridge or an eccentrically loaded bridge had a weak torsional capacity compared to an ordinary PSC box girder bridge due to the open cross-sectional characteristic of HTB. Therefore, three types of girders with different joint system between truss web member and concrete slab were tested for their torsional capacity. In this study, the three different types of HTB girders under torsional loading were simulated using FEM analysis to investigate the torsional behavior of HTB girders more in detail. The results are discussed in detail in the paper.