• 한국엔터테인먼트산업학회논문지
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• 제13권5호
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• pp.37-46
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• 2019

<창조 101>은 중국 텐센트가 한국 <프로듀스 X 101> 프로그램의 제작권을 매입하여 만든 리얼리티 예능 프로그램이다. 이 프로그램은 양성 대상의 미완성성, 양성 목표의 지향성, 과정의 성장성, 결정자의 국민성 등 네 가지 특징을 가지고 있다. 기획사에서 연습생을 키우는 과정을 프로그램을 통해 연습생 개인의 후면영역(back region)과 연예계의 후면영역을 전면영역(front region)으로 전환시켜 대중들에게 공개하였다. 이는 시청자들에게 기분 전환(diversion), 대인관계(personal relation) 유지와 자아 인정(personal identity) 등 효용 가치가 있으므로 주목과 인기를 많이 얻었다. 이러한 아이돌 양성 프로그램은 오디션 리얼리티 예능 프로그램의 발전에 좋은 귀감을 제공해 주었다.

• 한국콘텐츠학회논문지
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• 제18권7호
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• pp.503-518
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• 2018

본 연구는 2016년 한국에서 인기가 가장 많은 오디션 리얼리티 프로그램 <프로듀스 101> 시즌2를 대상으로 경쟁과 육성에 대한 내용을 중심으로 서사분석을 통해서 분석한다. 구체적인 제작 방식, 제작 방식을 설정하는 이유와 의의, 그리고 이 프로그램의 성공한 경험과 장점을 연구를 통하여 밝혀보고자 한다. 이를 위해서 서사 분석 이야기 층위에서 <프로듀스 101> 시즌2 제작 중 경쟁 방식, 육성 방식, 그리고 시청자의 위치를 서사구조로 분석한다. 분석 결과를 보면, <프로듀스 101> 시즌2는 생존과 방출을 중심으로 아이돌 그룹을 육성 하기 위한 오디션 프로그램으로 먼저 경쟁과 육성의 중요성을 강조한다. 또한, 시청자의 참여는 이 프로그램에 전체적으로 영향을 준다. 순위발표식에서 나타나는 시청자의 100% 선택권과 다른 결정적인 참여 형식은 <프로듀스 101> 시즌2의 시청자가 엄청 큰 권력을 가진다고 강조한다.

• Biomolecules & Therapeutics
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• 제2권4호
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• pp.331-335
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• 1994

As a part of the safety evaluation of Pseudomonas vaccine(CFC-101), antigenicity tests were carried out in guinea pigs and mice. In active systemic anaphylaxis(ASA) test, guinea pigs showed no sign or only moderate sign(1/5) when sensitized and challenged with up to 200 $\mu\textrm{g}$/kg. In homologous passive cutaneous anaphylaxis(PCA) test using guinea pigs, inoculation of CFC-101 alone did not produce CFC-101-specific antibody. When inoculated with 200 $\mu\textrm{g}$/kg plus adjuvant, challenge of 200 $\mu\textrm{g}$/kg produced PCA titer of 32(5/5) but challenge of 20 $\mu\textrm{g}$/kg did not produce CFC-101-specific antibody. In heterologous PCA test using mice, CFC-101-specific antibody was not detected when sensitized with CFC-101 alone. Some animals(3/12) showed positive PCA response when inoculated with 200 $\mu\textrm{g}$/kg plus alum. In passive hemagglutination (PHA) test, although no antibody was detected at 20 $\mu\textrm{g}$/kg, inoculation of 200 $\mu\textrm{g}$/kg alone or with alum produced positive response in all animals. This result has already been predicted because CFC-101 is a vaccine developed for the purpose of immunization. From the above results, it can be concluded that there is no adverse antigenic potential up to 10 times clinical dose of 200 $\mu\textrm{g}$/kg.

• 지식경영연구
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• 제20권3호
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• pp.155-171
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• 2019

In the entertainment industry which has great uncertainty, it is essential to predict public preference first. Thanks to various mass media channels such as cable TV and internet-based streaming services, the reality audition program has been getting big attention every day and it is being used as a new window to new entertainers' debut. This phenomenon means that it is changing from a closed selection process to an open selection process, which delegates selection rights to the public. This is characterized by the popularity of the public being reflected in the selection process. Therefore, this study aims to implement a machine learning model which predicts the winner of , which has recently been popular in South Korea. By doing so, this study is to extend the research method in the cultural industry and to suggest practical implications. We collected the data of winners from the 1st, 2nd, and 3rd seasons of the Produce 101 and implemented the predictive model through the machine learning method with the accumulated data. We tried to develop the best predictive model that can predict winners of by using four machine learning methods such as Random Forest, Decision Tree, Support Vector Machine (SVM), and Neural Network. This study found that the audience voting and the amount of internet news articles on each participant were the main variables for predicting the winner and extended the discussion by analyzing the precision of prediction.

• 미생물학회지
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• 제32권2호
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• pp.126-131
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• 1994

4-Chlorobiphebyl(4CB)를 분해한 Pseudomonas sp. DJ-12가 가지고 있는 pcbABCD 유전자를 Escherichia coli에 클로닝한 결과, 재조합 균주인 E. coli CU1과 CU101은 모두 Pseudomonas sp. DJ-12에서와 같이 4CB를 분해하여 2,3-dihydrozybiphenyl(2,3-DHBP)을 생성하는 탈염소화 기능을 보여주었다. 특히 pcbAB를 포함하는 재조합 플라스미드인 pCU101을 가지고 있는 E. coli CU101은 Pserudomonas sp. DJ-12에서와 같이 4CB로부터 4-chlorobenzoic acid를 생성하지 않고 2, 3-DHBP만을 생성하는 탈염소화 기능을 보여주었다. 그러므로 Pseudomonas sp. DJ-12의 염색체 DNA로부터 클로닝한 약 2.2kb의 pcbAB 유전자는 4CB로부터 2,3-DHBP만을 생성하는 탈염소화기능을 가지고 있음이 밝혀졌다.

• The Plant Pathology Journal
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• 제24권4호
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• pp.469-473
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• 2008

We have previously obtained a representative isolate KU101 of the predominant Penicillium species from rice under indoor storage conditions. In this study we attempted to characterize isolate KU101 using its morphological and molecular characteristics. When the micro- and macroscopic characteristics of isolate KU101 were compared with the P. islandicum reference isolate KCCM 34763, isolate KU101 was generally identical to those of isolate KCCM 34763, however, isolate KU101 grew faster and produced more orange to red pigments than isolate KCCM 34763. In a molecular-based identification, the nuclear sequence of the ITS1-5.8S-ITS2 region of isolate KU101 was most closely related to that of P. islandicum. Therefore, these results indicated that isolate KU101 from stored rice could be identified as P. islandicum, some isolates of which are known to produce mycotoxins.

• 한국수정란이식학회지
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• 제23권1호
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• pp.51-57
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• 2008

The present study investigated the efficient methods to produce in vitro Hanwoo embryos, and to improve the pregnancy rate. The developmental rate, total cell number and ICM ratio of in vitro embryos were compared amongst different culture media. Comparisons were also made on the status of recipients, pregnancy rate along with day of transfer after the estrus. Development of embryos into blastocyst stage in IVMD101 supplemented with 5% fetal bovine serum (FBS) group was significantly higher (34.2%) than that of TCM-199 supplemented with 5% FBS (26.8%) and IVMD101 without FBS (25.9%) (p<0.05). The development rate to blastocyst stage was significantly faster in IVMD101(5% FBS) than that of other groups ($0.2{\sim}2.3%$) (p<0.05). The average number of inner cell mass and trophectoderm were similar among treatment groups, which were $36.0{\sim}44.7$ and $83.3{\sim}106.7$. However, total cell number in IVMD101(5% FBS and 0% FBS) was significantly higher than that of TCM199(5% FBS). There were no differences in the pregnancy rate among treatment groups (32.0%, 33.9% and 28.6%, respectively). However, the pregnancy rate of Day 6 embryos cultured in IVMD101(5% FBS) was significantly (p<0.01) higher than IVMD101 without FBS and TCM-199 + 5% FBS (38.0% vs. 17.2% and 32.4%, respectively). No significant difference was observed for the pregnancy rate between heifer and cow transferred with Day 6 embryos cultured in IVMD 101(5% FBS) (42.7% and 39.3%, respectively). However, there was a significant difference of pregnancy rate (p<0.05) in heifer between one and two embryos transferred (31.4% and 41.9%). There was no difference of pregnancy rate among transfer days after estrus between heifer and cow, but the pregnancy rate of transfer to heifer with day 6 after estrus was significantly higher (p<0.05) than that of day 7 and 8 (22.2% vs. 49.0% and 38.7% respectively). Based on the above findings, there is a possibility to produce in vitro produced embryos cultured in IVMD101(5% FBS) showed higher blastocyst rate and the increased cell number. In terms of the pregnancy rate of in vitro produced embryos, the highest pregnancy rate was observed when two embryos were cultured in IVMD101(5% FBS) and transferred.

• Bulletin of the Korean Chemical Society
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• 제35권6호
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• pp.1659-1664
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• 2014

A porous metal-organic framework (MOF), MIL-101, was synthesized in the presence of sulfated zirconia (SZ) to produce acidic SZ/MIL-101 composites for the first time. The composites were characterized with XRD, nitrogen adsorption, FT-IR, scanning electron microscope, chemical analysis and so on. The composites (SZ/MIL-101s) were successfully applied in a liquid-phase esterification for a high yield of ester. This catalytic result of SZ/MIL-101, compared with that of pure SZ or MIL-101 (showing a negligible yield of ester), suggests that the SZ in the composite is highly active in the acid catalysis probably because of the well-dispersed active species of SZ. Moreover, the esterification is catalyzed in heterogeneous mode as confirmed by negligible esterification after filtration of the catalyst. Finally, microwaves can be efficiently applied both in the synthesis of the composites and the esterification reaction to accelerate the two processes of synthesis and esterification by about 5 times.

• Journal of Microbiology
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• 제37권1호
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• pp.45-49
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• 1999

To investigate the mechanism of cadmium tolerance in a cadmium-resistant Azomonas agilis PY101 that produces a specific fluorescent pigment promoted by cadmium, we carried out Tn5 mutagenesis and isolated four pigment-deficient mutants. In these mutants, Ppg1, Ppg2, and Ppg3 remarkably reduced the pigment production to 15.3%, 11.2%, and 13.9%, respectively. Especially, Ppg4 mutant did not produce the pigment at all. None of the mutants grew in the presence of 1500 ppm of CdCl2 in growth medium, and they exhibited differential sensitivities to cadmium. Ppg1, Ppg2, Ppg3, and Ppg4 mutants were sensitive to 900 ppm, 1100 ppm, 1000 ppm, and 800 ppm of CdCl2, respectively. These mutants also showed noticeable increase, from 8.8-fold to 13.2-fold, in the size of growth inhibition zone compared with that of the will type after treatment with cadmium. Therefore, the pigment production of A. agilis PY101 was found to decrease the toxic effects of cadmium to the bacterium.

• 미생물학회지
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• 제28권2호
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• pp.174-179
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• 1990

L-Phenylalanine을 대량생산하는 균주를 얻기 위하여 Escherichia coli K-12로부터 여러대사조절 변이주를 분리하였다. MWEC 83은 L-phenylalanine을 7.4 g/l 생산하는 tyrosine, tryptophan 이중 영양요구성 변이주이다. Tyrosine과 tryptop phan의 첨가없이 L-phenylalanine을 생산하기 위하여 MWEC 83으로부터 복기변이주 MWEC 101을 분리하였다. 또한 MWEC 101 균주로부터 여러 analog와 valine 내성주를 분리하였다. MWEC 101-5는 포도당 15%로 배양 54시간에 17.9 g/l의 L-phenylalanine을 생산하는 최고 우량균주이다. MWEC 101-5의 chorismate mutase와 prephenate dehydratase 효소환성은, 효소반응 혼합액 속에 2mM phenylalanine에 대하여 효소활성이 저해되지 않았다.