• Korean Journal of Animal Reproduction
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• v.9 no.2
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• pp.140-147
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• 1985

Seventy four Holstein heifers were randomly assigned over three trials to PRID-7+PG-6 and Synchromate B-9 regimens to synchronize estrus cycle for embryo transfer. Sexual behaviors; moounting, standing, orientation, chin-resting, sniffing, licking, rubbing and butting, vaginal swelling and mucus discharge were observed between 06-08, 12-14 and 18-20 h on 1st day and 00-02, 06-08, 12-14 and 18-20 h on 2nd day after removal of hormones. Synchromate-B treatment (81.6%) showed higher synchronized estrus rate than PRID treatment (77.8%) during observation period. Standing estrus was observed within 74 h after PG injection in PRID and within 52 h after removal of implant in Synchromate-B. About 68% of heifers in PRID and 74% of heifers in Synchromate-B showed standing estrus between 0-14 h on 2nd day after removal of the hormones. Synchromate-B resulted in a tighter synchrony of standing estrus than PRID. Incidence of average mounting and standing per head during observation period was 22.3 and 16.6 in PRID and 28.1 and 13.6 in Synchromate-B. The PRID showed peak in active mounting at 18-20 h on 1st day, however, the Synchromate-B showed at 0-2 h on 2nd day after removal of hormone. Active standing was shown between 18 h on 1st day to 20h on 2nd day in PRID, however, between 0-14 h on 2nd day after removal of hormone is Synchromate-B. There was slight difference in pattern of active mounting and standing during estrus between PRID and Synchromate-B. Conception rate of synchronized heifers transferred with fresh and frozen embryos by non-surgical and surgical methods was higher in synchromate-B (62.5%) than in PRID (38.5%). Chin-resting showed highest incidence among 6 sexual behavioral components in the both treatments. Synchromate-B showed higher incidence of chin-resting (16.6) than PRID (10.7). Synchromate-B group showed also higher incidence of orientation, sniffing and butting than PRID group. Synchromate-B resulted in more active sexual behaviors than PRID. The pattern of incidence of chin-resting, licking and butting was almost symmetrical in PRID with their peak values at 6-8 h on 2nd day, however in Synchromate-B chin-resting and sniffing was symmetrical with their peak values at 12-14 h on 2nd day after removal of hormone. There was tendency to increase vaginal swelling according to time passage of synchronized estrus in the both treatments. Incidence of mucus discharge in Synchromate-B was slightly higher than in PRID. Twenty to 40% was false negative in conception rate by tall painting before re-estrus day as judged by rectal palpation.

• Journal of Veterinary Clinics
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• v.1 no.1
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• pp.25-31
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• 1984

The effects of progesterone releasing intravaginal device (PRID) on the fertility levels in dairy cows were studied in 2 experiments. In experiment I, 70 lactating cows at 45 days postpartum were allotted to 3 groups and the treatments imposed were either： 1: Untreated control, 2: PRID with a capsule containing long of oestradiol benzoate (ODB) attached, inserted for 12 days, 3: PRID inserted for 12 days with long of prostaglandin F$\_$2${\alpha}$/ administration 24 h before PRID removal. Treated cows were inseminated 56 h after PRID removal and at an observed oestrus during the subsequent 48-day period. The control group was inseminated at an observed oestrus during this 60-day period. In experiment II, 60 ovarian disorder cows were divided into 5 groups and PRID+ODB inserted for 12 days. 1: atrophied ovary, 2: smooth ovary, 3: persistent corpus luteum, 4: follicular cyst, 5: luteal cyst. Treated cows were inseminated 56 h after PRID removal and at an observed oestrus over a period from the first insemination to 46 days. The results obtained were as follows: 1. The device produced a vaginal discharge in some animals. In experimenet I : 2. For treatments 2 and 3, respectively, conception rates to the fixed time insemination were 45% and 52%. 3. The conception rates of cows inseminated to the fixed time insemination and at an observed oestrus during a 60-day period were 65%, 86% and 91% for control, treatment 2 and 3, respectively. 4. Mean interval from calving to conception and inseminations per conception were 133, 91 and 86 days and 2.4, 2.1 and 1.9 for control, treatments 2 and 3, respectively. In experiment II； 5. The conception rates to the fixed time insemination for each group 20, 50, 70, 20 and 50%, respectively. 6. The total conception rates for the 48 days period of each group were 60, 70, 100, 60 and 90%, respectively. 7. The inseminations per conception of each group were 2.8, 2.1, 1.4, 2.7 and 1.9, respectively.

• Asian-Australasian Journal of Animal Sciences
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• v.16 no.9
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• pp.1268-1273
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• 2003

Sixty postpartum lactating Friesian cows in 3 treatments at a commercial dairy farm were used to study the effect of using progesterone supplementation with GnRH and PGF2$\alpha$ synchronization with and without timed AI on fertility during summer. Cows in treatment1($Tr_1$) and treatment2 ($Tr_1$) were fitted with progesterone releasing intravaginal device (PRID) device and injected with 10 g GnRH agonist on $51{\pm}3$ d postpartum (pp). Seven days later, PRID was removed and cows received 25 mg PGF2$\alpha$. Two days later, $Tr_1$ cows received another injection of 10 g GnRH and timed AI 16-20 h later. Control cows received only 25 mg PGF2$\alpha$ $58{\pm}3d\;pp$. $Tr_2$ and control cows were AI at detected estrus. Serum progesterone for all cows was determined on days of injection, AI and 21, 23 and 28 d postinsemination. Pregnancy rates from first AI based on serum P4 concentrations on d 21, 23 and 28 postinsemination (50, 40 and 35%) and that based on rectal palpation 40-45 d postinsemination (30, 15 and 15% for $Tr_1$, $Tr_2$ and control cows, respectively) did not differ among the three groups. Whereas, pregnancy rate at 120 d pp for $Tr_1$ (65%) was higher (p<0.05) than that in $Tr_2$ (30%) or control (30%). The overall pregnancy rate was not significantly different (90, 90 and 75% for $Tr_1$, $Tr_2$ and control, respectively). Days open for cows in $Tr_1$ ($100.3{\pm}9$) was less (p<0.03) than that in $Tr_2$ ($130.9{\pm}9$) or control ($135.1{\pm}10$). Results indicate that using PRID device with Ovsynch program had significantly increased pregnancy rate and decreased days open compared to AI at detected estrus after synchronization with GnRH, PRID and PGF2$\alpha$ or synchronization with one injection of PGF2$\alpha$.

• Proceedings of the KSAR Conference
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• 2002.06a
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• pp.75-75
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• 2002

한우의 사육규모가 다두화 된 농가에서 전년도 발정제어 처리에 따라 수태한 임신우의 분만내역과 차기번식에 미치는 영향을 규명하고자 수행하였다. 1. 발정유기방법별 계절번식처리우의 분만율은 PGF₂α구에서 73%(73/100), PRID 구에서 73.3%(22/30), CIDR 구에서 76.6%(23/30), GnRH-PGF₂α-GnRH 처리구에서 81%(81/100)였으며 전체적으로 76.5%(199/260)로 나타났다. 2. 계절번식농가의 자연발정 수정구에서 암송아지는 23.9㎏, 수송아지는 26.2㎏인 반면에 발정유기구에서는 암송아지는 24㎏, 수송아지는 24.9㎏로써 생시체중에 거의 차이를 보이지 않았고, 4개월령 체중에서 자연발정 수정구에서 암송아지 72㎏, 수송아지 75㎏인 반면에 발정유기구에서는 암송아지 75.6㎏, 수송아지 78.3㎏으로 발육상에 특별한 차이가 나타나지 않았다. 3. 다두사육농가의 자연발정 수정구에서 송아지 육성율은 86.5%(251/290)이였으며, 발정제어처리구에서는 87.0%(175/201)로써 유사한 경향을 나타내었으나 농가의 사육경험에 따라 6년 이상에서는 84.0%(105/125)였고, 10년 이상에서 88.4%(146/165)로써 사육경험이 많을수록 육성율이 우수하였다. 4. 발정유기방법별 분만후 발정재귀일수는 대조구에서 80.7일, PGF₂α 구에서 92.3일, PRID 구에서 78.5일, CIDR 구에서 64.5일, GnRH-PGF₂α-GnRH 처리구에서 65.6 일로 나타났고 분만 후 수태일수는 대조구에서 137.1일, PGF₂α 구에서 147.6 일, PRID구에서 141.3일, CIDR구에서 116.6일, GnRH-PGF₂α-GnRH 처리구에서 118일로 나타났으며 평균 134.3일이었다.

• Proceedings of the KSAR Conference
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• 2003.06a
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• pp.86-86
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• 2003

한우의 사육규모가 커짐에 따라 농가에서 암소의 번식관리는 더욱 어려워지게 되었으며 이를 효율적으로 조절하고자 다양한 발정제어 처리에 따라 수태시켰으며 이에 따라 임신한 한우번식우의 분만내역과 다음번 번식에 미치는 영향을 규명하고자 수행하였다. 1) 발정유기 방법별분만율은 PeGF$_2$$\alpha$구에서 73%(73/100), PRID구에서 73.3%(22/30), CIDR구에서 76.6%(23/30), GnRH-PGF$_2$$\alpha$-GnRH 처리구에서 81%(81/100)였으며 전체적으로 76.5%(199/260)로 나타났다. 2) 생시체중은 자연발정구에서 암송아지는 23.9kg, 수송아지는 26.2kg인 반면에 발정유기구에서는 암송아지는 24kg, 수송아지는 24.9kg으로써 거의 차이를 보이지 않았고, 4개월령 체중에 있어서도 자연발정구에서 암송아지 72kg, 수송아지 75kg인 반면에 발정유기구에서는 암송아지 75.6kg, 수송아지 78.3kg으로 발육상에 차이가 없었다. 3) 송아지 육성율은 자연발정구에서 86.5%(251/290)이었으며, 발정유기구에서는 87.0% (175/201)로써 유사한 경향을 나타내었으나 농가의 사육경험에 따라 6년 이상에서는 84.0% (105/125)였고, 10년 이상에서 88.4%(146/165) 로써 사육 경험이 많을수록 우수하였다. 4) 분만후 발정재귀일수는 대조구에서 80.7일, PGF$_2$$\alpha$구에서 92.3일, PRID구에서 78.5일, CIDR구에서 64.5일, GnRH-PGF$_2$$\alpha$-GnRH 처리구에서 65.6일로 나타났고 분만 후 수태일수는 대조구에서 137.1일, PGF$_2$$\alpha$구에서 147.6일. PRID구에서 141.3일, CIDR구에서 116.6일, GnRH-PGF$_2$$\alpha$-GnRH 처리구에서 118일로 나타났다.

• Journal of Embryo Transfer
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• v.20 no.1
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• pp.49-53
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• 2005

This study was conducted to investigate the effects of repetition usage of various estrus synchronization and seasonal breeding in Hanwoo. Body weight(kg) at birth and weaning in calves produced by seasonal breeding are 24.0kg, 75.6kg in female, 24.9kg, 78.3kg in male, respectively. Changes of birth weight(kg) by parity in Hanwoo increased gradually. Raising rates of calves by raising experience of farmer higher than in more 10 years as $88.4\%$ and in more 6 years as $84.0\%$. Delivering rates by induced estrus methods in $PGF_{2}{\alpha}$, PRID, CIDR and $GnRH-PGF_{2}{\alpha}-GnRH$ were $87.0\%$, $87.3\%$, $91.6\%$ and $96.0\%$, respectively. Conception days of post-partum following to induced estrus methods in Control, $PGF_{2}{\alpha}$ PRID, CIDR and $GnRH-PGF_{2}{\alpha}-GnRH$ were 137.1, 147.6, 141.3, 116.6 and 118.0 days, respectively. The results show that repetition usage of various estrus synchronization were not effective on postpartum reproduction in Hanwoo.

• Proceedings of the KSAR Conference
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• 2001.03a
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• pp.84-84
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• 2001

한우 농가의 다두화 사육규모에 적합한 번식관리모델 제시 및 수태율 증진을 위한 발정제어 방법을 개발하고자 수행하였다. 1. 년중 번식구의 분만간격은 412.9일, 수태당종부횟수 1.76회에 반하여 계절번식 I구와 II구는 각각 370.4일과 1.51회, 376.5일과 1.48회로 나타났다. 2. 송아지 생산율에 있어서 년중번식구 78.2%(174/184), 계절번식 I구 71.7%(99/138)에 비해 계절번식 II구에서는 79.2%(172/217)로 효율이 다소 높았다. 3. 발정유기방법별 동기화율에 있어서 PGF$_2$$_{\alpha}$ 2회 투여법에서 68.1%(141/207), PRID 삽입법 71.42%(15/21), CIDR 삽입법 86.3%(33/38)에 비해 GnRH-PGF$_2$$_{\alpha}$-GnRH 처리법은 93.1%(216/232)로 효율이 가장 높았다. 4. 발정유기방법별 1회 수정수태율은 PGF$_2$$_{\alpha}$ 2회 투여법 55.1%(64/l16), PRID 삽입법 54.0%(20/37), CIDR 삽입법 58.6%(17/29), 일괄수태법 58.8%(60/102)로 나타났고, 수태율은 각각 75%(87/116), 81%(30/37), 89.6%(26/29), 91.1%(93/102)였다. 5. 일괄수태 처리후 수정시점에 따른 수태율은 최종 GnRH 투여후 16~20 시간구가 65.3%로 가장 좋았다.

• Journal of applied mathematics & informatics
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• v.6 no.1
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• pp.217-226
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• 1999

In this paper we define automata-linearly independence. An automaton M has a basis B iff M is free provided that we assume that the action of S on X $\times$ S is (x,sa) for all a, s $\in$ S and x $\in$ X. if a semigroup S is PRID every subautomaton of a free S-automaton is free.

• Asian-Australasian Journal of Animal Sciences
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• v.20 no.11
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• pp.1670-1676
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• 2007

Fifty repeat breeder (RB) Friesian cows were allocated to five groups of 10 cows each, to determine the effect of progesterone (P4) supplement on P4 concentrations and pregnancy rates during the periods of corpus luteum (CL) formation and development between days 2-7 and 7-12 following a spontaneous or $PGF_{2{\alpha}}$-induced estrus. Cows were artificially inseminated during $PGF_{2{\alpha}}$-induced (PGF-P4-d2 and PGF-P4-d7 groups) or spontaneous (S-P4-d2, S-P4-d7, and control groups) estrus. Progesterone-releasing intravaginal device (PRID) devoid of estrogen capsule were inserted either on d 2 (PGF-P4-d2 and S-P4-d2 groups) or d 7 (PGF-P4-d7 and S-P4-d7 groups) post-insemination and left in place for 5 days. Control cows did not receive any treatment. Blood samples were collected for progesterone analysis from all cows once daily for 4 days starting on the day of estrus (d 0) and once every 3 days thereafter until d 22. Progesterone treatment by day interaction accounted for higher plasma P4 in treated than non-treated control cows. Progesterone concentrations differed significantly (p<0.05) during metestrus (d 2 to d 7) but not during diestrus (d 7 to d 12). $PGF_{2{\alpha}}$ treatment, lactation number, service number or their interactions did not affect progesterone concentrations and pregnancy rates. Therefore, cows were grouped according to the day of P4 supplement irrespective of the $PGF_{2{\alpha}}$ treatment. Progesterone supplement on d 7 but not d 2 significantly increased (p<0.03) pregnancy rates in repeat breeding cows with four or more previous services but not in cows in their third service. In conclusion, post-insemination P4 supplement to repeat breeding cows with four or more previous services improved pregnancy rates and should be advocated when no specific reason for infertility is diagnosed. Further studies with larger numbers of repeat breeding cows under field conditions are needed to ascertain the findings of this study.

• Journal of Embryo Transfer
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• v.1 no.1
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• pp.35-49
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• 1986

Recipients are an integral part of embryo transfer and they are expensive to maintain as a good recipient. Recipient management is one of the most important components in a successful embryo transfer program. Management includes selection and subsequent care of the animals. A good recipient is basically on "open" cows or heffers whose reproductive tract is capable of receiving one or two embryos and incubating it to term. Potential recipients should be always be healthy and cycling normally ranging from 18 to 23 days. A thorough veterinary examination is recommended for candidate of recipients and cattle for questionable health should be eliminated from the recipient herd. Age and size of recipients are particularly important considerations when heifers are used, because of most embryos available for transfer are from large dams and sires. Body condition can influence a recipient's production, reproduction and health. Obese and underconditioned cattle should be avoided for use. Transfer of fresh embryos especially requires precise synchronization of donors and recipients. For estrus synchronization, PGF$_2$$\alpha$ is injected twice 10 to 12 days apart and short4erm progestagen treatment is applied to potential recipient cattle by coil into vagina (PRID) or ear implant (Synchro-Mate-B). The highest pregnancy results are achieved in recipients at exact synchrony with donors or 12 to 24 hr earlier than donors. Estrus detection is a major factor in breeding efficiency. High accuracy can be achieved by use of heat mount detection alds or by obserbing cattle for 30-minute peroids 3 times daily. Assay progesterone in milk can be used to discrIminate between pregnant and nonprenant recipients. Rectal palpation on day 35 to 70 after is an accurate and safe method of pregnancy diagnosis. Embryonic mortality in recipients may be associated with factors such as high environmental temperature and nutritional or lactational stress in early lactation period. Achievement of short calving interval requires concentrated management activity during the first 90 days following calving. Acceptable candidate for a recipient should be routinely vaccinated for infectious diseases. Proper nutritional programs according to NRC requirements and body condition scoring system for recipient cattles are vital to the ultimate success of an embryo transfer program.r program.