The structural characteristics of Antheraea yamamai and Antheraea pernyi silk were investigated by using x-ray diffraction method, IR spectroscopy and polarizing microscopy. The amino acid composition, fiber density, thermal decomposition temperature and glass transition temperature were also measured for relating these physical properties to the structure in comparison with those of Bombyx mori silk fiber. There was no significant structural difference between A. yamamai and A. pernyi silk fiber on an examination of x-ray diffraction curve and IR spectrum. Both of these wild silk fibers showed double diffraction peaks at the Bragg angle 2Θ16.7˚ and 20.5˚by x-ray diffraction analysis as well as IR absorption peaks for the bending vibration of specific groups related to ala-ala amino acid sequence. On the other hand, the x-ray diffraction curve and IR spectrum of Bombyx mori silk fiber are different from those of wild silk fibers, indicating different crystal structure as well as amino acid sequences. It showed under the polarizing microscope examination that the birefringence and optical orientation factor of wild silk fibers are much lower than those of B. mori silk. Also, the surface of degummed wild silk fibers was characterized by the longitudinal stripes of microfibrils in the direction of fiber axies. The amino acid composition, which is strongly related to the fine structure and properties, was not significantly different between these two wild silk fibers. However, the alanine content was somewhat less and polar amino acid content more for A. yamamai. As a result of fiber density measurement, the specific gravities of B. mori, A. pernyi and A. yamamai were 1.355~1.356, 1.308~1.311, 1.265~1.301g/㎤ in the order, respectively. The calculated crystallinity(%) was 64% for B. mori and 51~52% for wild silk fibers, which showed same trend by IR method in spite of somewhat higher value. The thermal decomposition behaviour was examined by DSC and TGA, showing that the degradation temperature was in the order of B mori, A. prernyi and A. yamamai at around 350$^{\circ}C$. It was also observed by TGA that the decomposition seems to proceed step by step according to their specific regions in the fiber structure, resulting the difference in their thermal stabilities. The glass transition temperature was turned out to be 220$^{\circ}C$ for B. mori, 240$^{\circ}C$ A. yamamai and 255$^{\circ}C$ A. pernyi by the dynamic mechanical analysis. It is expected that the chemical properties are affected by the dynamic mechanical behavior in accordance with their structural characters.
The effect of water temperature and photoperiod on the oxygen consumption of the fasted juvenile parrot fish, Oplegnathus fasciatus was investigated to provide empirical data for the early-stage culture management and bioenergetic growth model of the species. The mean body weight of the juvenile used for the experiment was $21.5{\pm}1.9g$, and the oxygen consumption rate was measured under four water temperatures (10, 15, 20 and $25^{\circ}C$) and three photoperiods (24L:0D, 12L:12D and OL:24D) with an interval of 5 minutes for 24 hours using a continuous flow-through respirometer. In each treatment three replicates were set up and 15 juveniles were totally involved. The oxygen consumption rates increased with increasing water temperature under all photoperiod treatments (P<0.001). Mean oxygen consumption rates at 10, 15, 20 and $25^{\circ}C$ ranged $202.1{\sim}403.4,\;306.7{\sim}502.2,\;536.7{\sim}791.0\;and\;879.9{\sim}1,077.4mg\;O_2\;kg^{-1}h^{-1}$, respectively. $Q_{10}$ values ranged $1.58{\sim}2.30$ between 10 and $15^{\circ}C,\;2.44{\sim}3.06$ between 15 and $20^{\circ}C\;and\;1.86{\sim}2.6y9$ between 20 and $25^{\circ}C$, respectively. Mean oxygen consumption rates of O. fasciatus were the highest in continuous light (24L:0D) followed by 12L:12D and 0L:24D (P<0.001). The oxygen consumption of fish exposed to the 12L:12D photoperiod was significantly higher during the light phase than during the dark phase under all temperature treatments (P<0.001). In summary, oxygen consumption rates of the juvenile parrot fish increase with increasing water temperature and lengthening daylight period; and, thereby, changes in water quality resulted from the depletion of oxygen under high temperature and long daylight photoperiod conditions should be monitored.
This study presents spatial characteristics of cloud using satellite image in the extreme heavy snowfall of the Yeongdong region. 3 extreme heavy snowfall events in the Yeongdong region during the recent 12 years (2001 ~ 2012) are selected for which the fresh snow cover exceed 50 cm/day. Spatial characteristics (minimum brightness temperature; Tmin, cloud size, center of cloud-cell) of cloud are analyzed by tracking main cloud-cell related with these events. These characteristics are compared with radar precipitation in the Yeongdong region to investigate relationship between cloud and precipitation. The results are summarized as follows, selected extreme heavy snowfall events are associated with the isolated, well-developed, and small-scale convective cloud which is developing over the Yeongdong region or moving from over East Korea Bay to the Yeongdong region. During the period of main precipitation, cloud-cell Tmin is low ($-40{\sim}-50^{\circ}C$) and cloud area is small (17,000 ~ 40,000 $km^2$). Precipitation area (${\geq}$ 0.5 mm/hr) from radar also shows small and isolated shape (4,000 ~ 8,000 $km^2$). The locations of the cloud and precipitation are similar, but in there centers are located closely to the coast of the Yeongdong region. In all events the extreme heavy snowfall occur in the period a developed cloud-cell was moving into the coastal waters of the Yeongdong. However, it was found that developing stage of cloud and precipitation are not well matched each other in one of 3 events. Water vapor image shows that cloud-cell is developed on the northern edge of the dry(dark) region. Therefore, at the result analyzed from cloud and precipitation, selected extreme heavy snowfall events are associated with small-scale secondary cyclone or vortex, not explosive polar low. Detection and tracking small-scale cloud-cell in the real-time forecasting of the Yeongdong extreme heavy snowfall is important.
Blue-ice area is a glacial ice field in ice sheet, ice shelf and glaciers where snow ablation and sublimation is larger than snowfall. As the blue-ice area has large influences on the meteorite concentration mechanism and ice mass balance, it is required to quantify the concentration of blue-ice. We analyzed spectral reflectance characteristics of blue-ice, snow and cloud by using MODIS images obtained over blue-ice areas in McMurdo Dry Valleys, East Antarctica, from 2007 to 2012. We then developed Normalized Difference Blue-ice Index (NDBI) algorithm which quantifies the concentration of blue-ice. Snow and cloud have a high reflectance in visible and near-infrared (NIR) bands. Reflectance of blue-ice is high in blue band, while that lowers in the NIR band. NDBI is calculated by dividing the difference of reflectance in the blue and NIR bands by the sum of reflectances in the two bands so that NDBI = (Blue-NIR)/(Blue + NIR). NDBI calculated from the MODIS images showed that the blue-ice areas have values ranging from 0.2 to 0.5, depending on the exposure and concentration of blue-ice. It is obviously different from that of snow and cloud that has values less than 0.2 or rocks with negative values. The change of NDBI values in the blue-ice area has higher correlation with snow depth ($R^2=0.699$) than wind speed ($R^2=0.012$) or air temperature ($R^2=0.278$), all measured at a meteorological station installed in McMurdo Dry Valleys. As the snow depth increased, the NDBI value decreased, which suggests that snow depth can be estimated from NDBI values over blue-ice areas. The NDBI algorithm developed in this study will be useful for various polar research fields such as meteorite exploration, analysis of ice mass balance as well as the snow depth estimation.
Back J. J.;Park C. K.;Yang B. K.;Kim C. I.;Cheong H. T.
Reproductive and Developmental Biology
/
v.29
no.3
/
pp.175-180
/
2005
This study was conducted to examine the effects of demecolcine-assisted enucleation and recipient cell cycle stage on the development of bovine somatic cell nuclear transfer (NT) embryos. In vitro cultured oocytes for $16\~20$ h were classified by first polar body (1st PB) extrusion and cell cycle stage (MI and MII) and treated $0.4\;{\mu}L/mL$ demecolcine for 40 min before enucleation. Enucleated oocytes were fused electrically with bovine ear skin cells, activated by Ca-ionophore+DMAP, and cultured in vitro. Most of eggs ($86.2\%$) treated with demecolcine protruded a chromosome mass and enucleated efficiently ($98.8\%$, (P<0.05). Demecolcine did not have a deteriorative effect on the development of NT embryos. Developmental rate of NT embryos reconstituted with oocytes extruded 1st PB significantly higher than that of NT embryos produced by oocytes without 1st PB ($18.2\%\;vs.\;4.6\%\cdot$, P<0.05). Cleavage and blastocyst formation rate of embryos reconstituted with MI oocytes ($69.4\%\;and\;5.9\%$, respectively) were significantly lower than those of embryos reconstituted with MII oocytes ($96.7\%\;and\;23.9\%$, respectively, P<0.05). From the present result, it is suggested that domecolcine is useful for the enucleation of recipient oocytes in bovine NT procedures, and MII oocytes rather than MI oocytes are more appropriate for recipient cytoplasm. Although, the potential to develop into blastocysts of NT embryos produced by 1st PB-nonextruded and MI oocytes was very low, these oorytes could be used for NT.
According to the Montreal Protocol, CFC 113, one of the ozone-depleting substances, will be prohibited to use as a cleaning solvent essentially in the electronic industry. Therefore, the development of the alternative cleaning solvents to CFC 113 is being accelerated. A number of the alternative cleaning solvents are avialable on the market. The alternatives of Axarel 32(DuPont), Cleanthru 750H(KAO Chemical), and EC-Ultra(Petroferm) are chosen for the comparison of cleaning performance with CFC 113. The test methods for measuring the cleaning performance were composed of the measurement of the physical properties, the experiments on the material compatibility with cleaning solvents, the measurement of the evaporation rate, and finally the experiments of the removal efficiency. Normally the basic physical properties of the alternatives had higher boiling points, viscosity and surface tension, which were quite different to those of CFC 113. In terms of solubility of rosin-based flux, the solubilities of abietic acid (nonpolar organic) were similar, but those of the activator (polar organic) in the alternatives were better than CFC 113. The evaporation of the alternatives was very slow, compared to CFC 113, which had much lower boiling point. All the cleaning solvents showed the good material compatibility with FR4 and Cu-coated PCB. The better removal efficiencies of abietic acid were obtained when using the ultrasonic mechanical energy over the dipping method. The experiments also indicated the very slow-eavaporating solvent was not desirable with the dipping cleaning method, and the differences in the removal efficiency of the alternatives with the ultrasonic cleaning method were negligible. Among the alternatives, the overall cleaning performances were obsorved as almost similar. Before selecting the ultimate cleaning solvent, the application of cleaning machine, environmental issues, and economics are simultaneously considered with the cleaning performance.
The faunal assembalges of macrobenthos and their habitat conditions on the soft-bottoms around Dokdo(Dok Island) was investigated using a box corer and a van Veen grab in Sept. 1999 and May 2000. The sediments in the slope sites were composed of sand particles and those in Ullneung Basin were mud. The sediments in the shelf sites were in the range of fine to medium sand. The organic content of the slope sediments was in the range of 1 to 2%. The macrobenthos occurred at the slope sites represented by 15faunal groups belonging to 8 phyla, and the major faunal group was polychaetous annelids. They comprised ca. 80.6% in slope sites, and 84.8% in shelf sites. Dominant species in the slope were Exogone verugera(40.9%), Cossura longocirrata (8.4%), Tharyx sp. (6.6%), Scalibregma inflatum (4.9%), Aedicira sp. (4.7%), Aricidea ramosa (3.8%), and Sigambra tentaculata (3.7%). Dominant species in the shelf were Chone sp. (49.3%), Tharyx sp. (18.4%), Ophelina acuminata (6.7%), Chaetozone setosa (3.8%), Glycera sp. (2.6%), and Aedicira sp. (2.4%). The mean densities of macrobenthos in the slope and shelf area were $2,028\;ind./m^2$ and $456\;ind./m^2$, respectively. The trophic composition of benthic polychaete worms in the slope area was different from that in shallow shelf area: surface deposit feeding worms were most abundant in slope area whereas filter feeding worms in shelf area. According to the cluster analysis and MDS plots, the spatial distribution of macrobenthos in Dokdo slope region was related with the sediment properties such as particle size and organic content. In the case of vertical distribution of macrobenthos in slope sites, most faunas concentrated in the upper sediment layer within 2cm depth.
CREAHS II carried out an intensive hydrographic survey covering almost entire East Sea in 1999. Hydrographic data from total 203 stations were released to public on the internee. This paper summarized the results of water mass analysis by OHP (Optimum Multiparameter) method that utilizes temperature, salinity, dissolved oxygen, pH, alkalinity, silicate, nitrate, phosphate and location data as an input data-matrix. A total of eight source water types are identified in the East Sea: four in surface waters(North Korea Surface Water, Tatar Surface Cold Water, East Korean Coastal Water, Modified Tsushima Surface Water), two intermediate water types (Tsushima Middle Water, Liman Cold Water), two deep water types (East Sea Intermediate Water, East Sea Proper Water). Of these NKSW, MTSW and TSCW are the newly reported as the source water type. Distribution of each water types reveals several few interesting hydrographic features. A few noteworthy are summarized as follows: The Tsushima Warm Current enter the East Sea as three branches; East Korea Coastal Water propagates north along the coast around $38^{\circ}N$ then turns to northeastward to $42^{\circ}N$ and moves eastward. Cold waters of northern origin move southward along the coast at the subsurface, which existence the existence of a circulation cell at the intermediate depth of the East Sea. The estimated volume of each water types inferred from the OMP results show that the deep waters (ESIW + ESPW) fill up ca. 90% of the East Sea basins. Consequently the formation and circulation of deep waters are the key factors controlling environmental condition of the East Sea.
In order to assess the ecological changes induced by organic pollutants of the Shihwa Lake, BPI (Benthic Pollution Index) based on the benthic faunal community was employed. It was modified from Infaunal Trophic Index (ITI), and recommended as a pollution detecting method for the environmental assessment. The BPI values were calculated from the benthos data, which were collected for three terms: in 1980, before the Shihwa Lake was built up; in 1994-1997, which the Shihwa Lake was completely isolated from the outer seawater; in 1997-1999, after inflow of the outer seawater. Since the Shihwa Dike was constructed in February 1994, the pollution intensity of the lake had been increased from the narrow and inner part of the former Gyeonggi Bay and spread fast along the coast line of the Shihwa Lake. Then, in 1996 it showed the very high BPI levels all around the Lake. This serious polluted condition had been lasted till 1997, when the inflow of the seawater was begun. In 1998, from the nearest part of the Shihwa Gate, the BPI levels gradually became low in most area of the Lake, except its inner and narrow part. These greatly lowered BPI levels mean that the seawater inflow could be assumed to affect positively in the lake. Furthermore, BPI gave the same results from the other environmental assessment based on the abundance and the species richness of macrobenthic community. It shows that BPI could be useful as an effective method to assess the marine environment and evaluate the status of environmental conditions.
The black scraper Thamnaconus modestus was a commercially important fish species in the 1980s, but suddenly its commercial significance decreased in the 1990s mainly due to continuous overfishing. Recently, in order to reverse the depleted stocks of the black scraper and help the species recover, seed production technology has emerged. This has led to the farming of the black scraper in several parts of the southern coast of Korea. Since detailed research on its metabolism in relation to water temperature has been scanty, this was the investigative focus of the present study. The standard metabolism rates of the black scraper (9-10 months old, total length=$22.6{\pm}0.8cm$, wet weight=$140.3{\pm}13.9g$) were measured at seven different water temperature settings (12, 15, 17, 20, 23, 26, $28^{\circ}C$) to understand the relationship between metabolism and water temperature. Relationships between water temperature (WT) and oxygen consumption rate (OCR) were obtained as SOCR (weight-specific oxygen consumption rate) = 0.0117WT - 0.0135 ($r^2=0.9351$) and IOCR (oxygen consumption rate per individual) = 1.8160WT - 5.4007 ($r^2$ = 9428). The $Q_{10}$ (temperature sensitivity), an indicator of the sensitivity of biological function to temperature, was analyzed. In our experiment, when the water temperature increased, the $Q_{10}$ value decreased. The $Q_{10}$ value was 6.27 in waters where the temperature ranged from $12-15^{\circ}C$ and this was much higher than the values obtained in waters where temperatures ranged between (1) $15-23^{\circ}C$ and (2) $23-28^{\circ}C$. Consequently, it was shown that the black scraper is a warm water species and inhabiting waters in the temperature range from $15-28^{\circ}C$ is deemed appropriate.
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