• Asian-Australasian Journal of Animal Sciences
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• v.14 no.5
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• pp.693-696
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• 2001

The effect of replacement of in vitro maturation medium completely with the buffalo follicular fluid (buFF) on in vitro oocyte maturation of buffalo oocytes was studied. 5 to 8 buffalo cumulus oocyte complexes were cultured in a single drop with each of the eight media studied i.e., M199+steer serum (10% v/v), M199+steer serum (10% v/v)+PMSG, M199+buFF (10% v/v), M199+buFF (10% v/v)+PMSG, M199+buFF (50% v/v), M199+buFF (50% v/v)+ PMSG, buFF (100%) and buFF+PMSG at $39^{\circ}C$ and 5% $CO_2$ in air for 24 h. Supplementation of M199 with Steer serum alone resulted in IVM rate of 35% only. When the above medium was supplemented with PMSG, the maturation rate rallied to 82%. Significant increase in the maturation rates were observed when M199 was supplemented with increasing levels of buFF. A further increase in the maturation rate was also obtained when PMSG was incorporated into the medium of M199 supplemented with buFF. The rate of maturation was to the tune of 91% when oocytes were matured in buFF alone which was increased non significantly on the addition of PMSG. Highest maturation rate (97%) obtained with M199+buFF (50%v/v)+PMSG did not differ significantly from that obtained by either M199+buFF (10%v/v)+PMSG or buFF+PMSG. It is suggested that buFF alone without any supplementation can form the effective in vitro maturation medium for buffalo oocytes.

• Asian-Australasian Journal of Animal Sciences
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• v.23 no.3
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• pp.308-311
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• 2010

An experiment was conducted using indigenous Kivircik ewes to evaluate the effect of intravaginal progestagen sponges, containing 30 mg of fluorogestone acetate (FGA), followed by administration of pregnant mare serum gonadotrophin (PMSG) on inducing synchronized oestrus in the season and fertility. Three times of PMSG administration relative to sponge withdrawal (24 h before (n = 30), at (n = 29) or 24 h after (n= 29)) and two routes of PMSG administration (intramuscular (n = 46) and subcutaneous (n = 42) were compared for estrous response, number of multiple births and fecundity rates. There were no significant differences in terms of estrous response, due to differences in the time and route of PMSG administration. Lambing percentage, proportion of multiple births and fecundity were 75.6, 51.6 and 114.6%, respectively. The administration had a significant effect on lambing (p<0.05), multiple birth and fecundity rates (p<0.01). The subcutaneous administration of PMSG resulted in a significantly higher lambing rate (p<0.05) and fecundity rate (p<0.01), compared to the intramuscular injection of the PMSG.

• Korean Journal of Animal Reproduction
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• v.13 no.1
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• pp.26-31
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• 1989

This study was carried out to investigate the effects of PMSG and/or HCG treatment on superovulation and fertilization in the golden hamster. The results obtained were summarized as follows: 1. The female groups treated with 30 IU PMSG or 30 IU PMSG＋25IU HCG ovulated more eggs than those treated with 15 IU PMSG or 15 IU PMSG＋25IU HCG(P<.01). All the PMSG treatment groups superovulated as compared with the untreated control group(P<.01). There were no differences on fertilization rate between the superovulated groups and the control group. 2. The fertilized ova were obtained only by the female group treated with 30 IU PMSG at 1000hr on day 1(morning of ovulation) of the estrous cycle. 3. The intervals between PMSG and HCG injection necessary to obtain the consistent superovulation and fertilized ova were 66hr and 72hr. 4. The superovulated ova were collected from oviduct 48hr, oviduct and uterus 72hr, and uterus 96 hr after mating. 80.3% of two cell, 75.8% of eight cell, and 73.7% of blastocyst of the ovulated ova occurred 48hr, 72hr, and 96hr after mating, respectively.

• Korean Journal of Animal Reproduction
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• v.7 no.1
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• pp.15-18
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• 1983

As a preliminary experiment to establish the process of embryo transfer in rabbit, present sutdies were carried out with 75 mature Japanese of ovary to pregnant mare's serum gonadotropin(PMSG) and human chorionic gonadotropin(hCG) and collection rate of embryos at various times after hCG injection. Female rabbits were superovulated using 50∼100IU hCG or 75∼100IU PMSG and 50∼751IU hCG injected 83hrs apart. The results obtained were as follows: 1. The average number of growth follicles obtained from all of rabbits treated with hCG or PMSG-hCG was 28.1. PMSG-hCG treatment group (30.9) was clearly increased more than hCG treatment group (16.7). 2. In ovulation score, PMSG-hCG treatment group (21.0) was increased more than hCG treatment group (7.9), showing the same trends in the growth of follicles. 3. The ovulation rate per follicles developed was higher in the rabbits treated with 100 IU PMSG and 75 IU hCG (18.9%) than that from the other groups. 4. The oviduct score (72.9%) was inclined to higher than that from uteri (57.1%) in score of embryo collection.

• Journal of Embryo Transfer
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• v.22 no.1
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• pp.39-45
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• 2007

This study was performed to induce the estrus in 9 anestrus Shih-tzu bitches by intramuscular injection of pregnant mare serum gonadotrophin (PMSG) 50IU/kg for 10 consecutive days and by intravenous injection of human chorionic gonadotrophin (hCG) 1,000IU/Head on Day 10. The day when the first injection of PMSG was counted as Day 0 of experiment. All of the bitches were monitored by vaginal discharges, displays the perineal region, vaginal swelling and male acceptances. The 9 bitches (100%) showed vaginal discharges and vaginal swelling, and were mated. The 5 bitches out of 9 bitches were pregnant (55.6%) and 4 bitches were non-pregnant (44.4%). The 3 bitches out of 5 pregnant bitches were spontaneously delivered (33.3%) and litter size were $1.66{\pm}1.15\;(1{\sim}3\;pups)$ pups. The 2 bitches were diagnosed as early embryonic death on days 38 and 41 after first injection of PMSG. These results indicated that rates of estrus induction, pregnancy and delivery were 100%, 55.6% and 33.3%, respectively, using PMSG and hCG.

• The Transactions of The Korean Institute of Electrical Engineers
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• v.57 no.6
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• pp.951-958
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• 2008

This paper describes development of hardware simulator for the PMSG wind power system, which was designed considering wind characteristic, blade characteristic and blade inertia compensation. The simulator consists of three major parts, such as wind turbine model using induction motor, PMSG generator, converter-inverter set. and control system. The turbine simulator generates torque and speed signals for a specific wind turbine with respect to given wind speed. This torque and speed signals are scaled down to fit the input of 2kW PMSG. The PMSG-side converter operates to track the maximum power point, and the grid-side inverter controls the active and reactive power supplied to the grid. The operational feasibility was verified by computer simulations with PSCAD/EMTDC, and the implementation feasibility was confirmed through experimental works with a hardware set-up.

• Journal of Veterinary Clinics
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• v.17 no.1
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• pp.285-288
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• 2000

Nine immature 30-day-old female rats were injected sc at 0800 hr with pregnant mare serum gonadotrophin(PMSG) to induce ovulation and mating. Fifty-six hours later the animals were placed with mature male rats overnight (one female and one male). Five of 9 immature female rats treated with PMSG were pregnant and allowed to maintain the pregnancy to term. Three of 5 pregnant rats were failed to maintain pregnancy to term. Two of 5 pregnant rats seemed to be developed normally and increased abdominal enlargement as pregnancy progresses, but did not occurred parturition on day of 43 or 48 of pregnancy, respectively. On day 44 or 49, pregnant rats were killed and examined uterus and ovaries. There was no fetus but approximately 50∼60ml. of mucopurulent fluids were accumulated in the uterine cavity and 40 or 42 corpora lutea persisted in the ovaries. Pyometra was developed after coitus in PMSG-treated immature female rat.

• The Transactions of The Korean Institute of Electrical Engineers
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• v.58 no.6
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• pp.1130-1137
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• 2009

This paper describes a scaled model development of PMSG wind power system using wind turbine simulator and matrix converter. The wind turbine simulator, which consists of an induction motor with vector drive, calculates the output torque of a specific wind turbine using simulation software and sends the torque signal to the vector drive after scaling down the calculated value. The operational feasibility of interconnected PMSG system with matrix converter was verified by computer simulations with PSCAD/EMTDC software. The feasibility of hardware implementation was conformed by experimental works with a laboratory scaled-model of wind power system. The simulation and experimental results confirm that matrix converter can be effectively applied for the PMSG wind power system.

• Asian-Australasian Journal of Animal Sciences
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• v.4 no.1
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• pp.1-5
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• 1991

Responsiveness of the testis to pregnant mare's serum gonadotropin (PMSG) was studied in immature Nili-Ravi buffalo bulls. Four month old bull calves weighing between 66 to 100 kg raised under uniform condition, were treated with 1000 IU PMSG or vehicle, daily, for six days. PMSG induced an increase in the size of the testis, enlargement of the seminiferous tubules and activation of the spermatogonia. The number of differentiated Leydig cells in the testis of gonadotropin treated animals increased considerably over that of the control testes. A significant increase in plasma testosterone concentrations was observed 24 h following the first injection of PMSG and the levels continued to increase until day 6. In vehicle treated animals plasma testosterone levels remained more or less at pretreatment values. The data suggest that buffalo bull testis is functionally responsive to gonadotropin at an early stage of prepubertal development.

• Korean Journal of Veterinary Research
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• v.34 no.4
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• pp.745-757
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• 1994

Superovulatory treatment with exogenous gonadotropins adversely affects the uterus through the disruption of the delicate balance of ovarian steroids (estrogens, progestins, androgens). To examine the uterine effects of this treatment, 189 rats were given 4IU, 20IU or 40IU pregnant mare',s serum gonadotropin(PMSG) at 28 days of age and sacrificed every 24h until day 10(D10) post injection. Long term uterine effects were examined in 12 rats treated with 4IU or 40IU PMSG and killed on D30. Adult rat uteri were examined to provide a reference for comparisons. Morphological and histological changes of control (4IU) uteri mimicked those of the adult on a comparable time-course form D2 to D5. Administration of superovulatory doses(20IU, 40IU) of PMSG produced stromal hypertrophy by D2 and focal papillary hyperplasia of the luminal epithelia by D3. Levels of $17{\beta}$-estradiol following 20IU and 40IU PMSG treatment were significantly(p<0.05, p<0.005) elevated above those of controls after D1. Androgen levels of both groups(20IU, 40IU) significantly p<0.05, p<005 increased from baseline on D1 and were maximum between D2 and D3. In the 20IU PMSG group, the hyperplasia gradually regressed after D3 and was absent by D10. The hyperplasia in the 40IU PMSG group, however, had become extensive by D6. It is suspected that preceding elevated levels of estrogen may be responsible for this progressive change. On D 4, the levels of $17{\beta}$-estradiol reached a maximum, which was significantly(p<0.001) greater than both the controls and 20IU PMSG-treated rats. Between D6 and D10, the hyperplasia in 40IU PMSG-treated rats partially regressed. Examination of uteri from D30 revealed no evidence of the hyperplasia. It is suggested that previous exposure to high levels of estrogen and androgens, secondary to superovulation, is possible cause for the observed hyperplasia.