• 한국발생생물학회지:발생과생식
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• 제17권4호
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• pp.345-351
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• 2013

This study examines the effects on fertilization rate (FR), hatching rate (HR), and normal individual rate after artificial fertilization using frozen thawed sperm according to the cryoprotectant (DMSO) concentration and the period of cryopreserved sperm of longtooth grouper, Epinephelus bruneus. Performing artificial fertilization using frozen-thawed sperm, after freezing the sperm at different DMSO concentration of 5.0%, 7.5%, 10.0% respectively, FR were (DMSO 5.0%: $99.5{\pm}0.8%$, DMSO 7.5%: $99.5{\pm}0.7%$, and DMSO 10.0%: $99.6{\pm}0.6%$). The results are not significantly different from the control fresh sperm (100%). HR also (DMSO 5.0%: $96.2{\pm}2.3%$, DMSO 7.5%: $95.3{\pm}3.6%$, 10.0%: $96.6{\pm}1.8%$) were not significantly different in each group. The normal individual rate after hatching using with control fresh sperm ($98.4%{\pm}0.5$) and DMSO concentration level of 5.0% ($97.8{\pm}0.1%$) were not significantly different. However, with 7.5% ($97.2{\pm}0.6%$) and 10.0% DMSO concentrations ($95.9{\pm}0.2%$) are lower than the normal individual rate after hatching observed in the control and 5.0% DMSO. Performing artificial fertilization using frozen-thawed sperm at different frozen period (2 days, 2 years, and 3 years), 10% DMSO FR and HR of 3 years (FR; $66.8{\pm}1.8%$, HR: $82.0{\pm}12.9%$) and 2 years (FR; $78.5{\pm}14.8%$, HR: $79.3{\pm}0.6%$) cryopreserved sperm were lower than control (FR; 100%, HR: $91.1{\pm}3.6%$) and 2 days cryopreserved sperm (FR; $99.6{\pm}0.6%$, HR: $96.6{\pm}1.8%$). These results suggest suitable DMSO concentration ranges of cryopreservation sperm for E. bruneus is 5 to 10% and with 2 to 3 years cryopreservation period, cryopreservation sperm can be useful for seed production.

• Asian Journal of Atmospheric Environment
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• 제8권1호
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• pp.25-34
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• 2014

In winter 2013, extreme air pollution by fine particulate matter ($PM_{2.5}$) in China attracted much public attention. In order to simulate the $PM_{2.5}$ pollution, the Community Multiscale Air Quality model driven by the Weather Research and Forecasting model was applied to East Asia in a period from 1 January 2013 to 5 February 2013. The model generally reproduced $PM_{2.5}$ concentration in China with emission data in the year 2006. Therefore, the extreme $PM_{2.5}$ pollution seems to be mainly attributed to meteorological (weak wind and stable) conditions rather than emission increases in the past several years. The model well simulated temporal and spatial variations in $PM_{2.5}$ concentrations in Japan as well as China, indicating that the model well captured characteristics of the $PM_{2.5}$ pollutions in both areas on the windward and leeward sides in East Asia in the study period. In addition, contribution rates of four anthropogenic emission sectors (power generation, industrial, residential and transportation) in China to $PM_{2.5}$ concentration were estimated by conducting zero-out emission sensitivity runs. Among the four sectors, the residential sector had the highest contribution to $PM_{2.5}$ concentration. Therefore, the extreme $PM_{2.5}$ pollution may be also attributed to large emissions from combustion for heating in cold regions in China.

• 한국대기환경학회:학술대회논문집
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• 한국대기환경학회 2004년도 추계학술대회 논문집
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• pp.421-422
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• 2004

• Journal of Pharmaceutical Investigation
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• 제18권1호
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• pp.1-3
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• 1988

Effect of hydrochlorothiazide on serum potassium level was studied in the hypokalemia-induced rats by the oral administration of glycyrrhiza extract (GE) for 4 weeks. According to the concentration of GE, serum potassium levels were found to be $5.8{\pm}0.2,\;5.4{\pm}0.2\;and\;5.5{\pm}0.2\;mM/l$ after oral administration of 0.1, 0.2 and 0.5% GE solutions for 4 weeks, respectively, comparing $6.4{\pm}0.2\;mM/l$ in normal rats. The i.p. administration of hydrochlorothiazide (100mg/kg) showed no significant difference $(5.1{\pm}0.3-5.2{\pm}0.3\;mM/l)$ in the decrease of serum potassium levels between these hypokalemia-induced rats and normal rats, regardless of the concentration of pretreated GE solutions. Therefore it was considered that the administration of hydrochlorothiazide did not worsen the hypokalemia induced by the long term administration of GE in rats.

• 대한원격탐사학회지
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• 제36권6_3호
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• pp.1733-1741
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• 2020

본 연구는 다변량 통계분석을 이용하여 다양한 기상인자가 대기 중 미세먼지(pariculate matter 10, PM10) 농도에 미치는 영향을 분석하였으며, 대기 중 PM10 농도에 따른 빗물 수질 변화를 평가하였다. 총 11회의 강우 이벤트를 대상으로 강우 전후의 대기 중 PM10 농도를 실시간 측정하였으며, 총 183개 빗물 샘플을 수집하여 pH와 EC (electrical conductivity)를 실시간 측정하고 양이온(Na+, Mg2+, K+, Ca2+, NH4+) 및 음이온(Cl-, NO3-, SO42-) 농도를 분석하였다. 측정된 데이터를 바탕으로 대기 중 PM10 농도, 기상인자, 빗물 수질 간 상관관계를 규명하기 위해 주성분 분석(principal component analysis, PCA)과 피어슨 상관분석(Pearson correlation analysis)을 실시하였다. 강우 유형 1(강우 강도: > 5 mm/h)의 경우, 누적 강우량에 따른 대기 중 PM10 농도는 유의한 음의 상관관계(r = -0.55, p < 0.05)를 보였으며 대기 중 PM10 농도는 빗물 내 수용성 이온(r = 0.25) 농도와 EC (r = 0.4)를 증가시키는 요인으로 작용하며, 빗물의 pH는 감소시키는 것으로 나타났다(r = -0.7). 반면, 강우 유형 2(강우강도: <5 mm/h)의 경우, 누적강우량과 대기 중 PM10 농도는 음의 상관관계를 보이지 않았으며, 대기 중 PM10 농도와 빗물 수질 간 통계적으로 유의한 상관관계가 나타나지 않았다.

• Asian Journal of Atmospheric Environment
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• 제7권4호
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• pp.209-216
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• 2013

This study focused on a comprehensive and detailed interpretation for the springtime air quality influenced by both artificial (particulate matter (PM) and asbestos) and biological (pollen) sources in Fukuoka Prefecture, Japan. An intensive measurement of PM was conducted at four characteristic sites (i.e., a heavy traffic area, a residential area, an industrial area, and a desolate area) in the Fukuoka Prefecture during spring of 2007. Analysis of major ionic species in $PM_{2.5}$ was performed by an Ion Chromatography, and asbestos and pollen were identified by Scanning Electron Microscopy with an energy dispersive X-ray spectrometer (EDX). $PM_{2.5}$ concentration ($65.3{\mu}gm^{-3}$) measured in an industrial area (site C) was extraordinarily high compared to those monitored in other areas; it greatly exceeded the Japan's $PM_{2.5}$ criteria (a daily average of $35{\mu}gm^{-3}$). NOAA's HYSPLIT dispersion model suggests that this high level of $PM_{2.5}$ monitored at site C is unlikely to affect the Asian continent. The ambient concentrations of $PM_{2.5}$-related anions ($NH_4{^+}$, $NO_3{^-}$, and $SO_4{^{2-}}$) and their relative contributions to $PM_{2.5}$ were also investigated in four study areas. The concentrations of these major water-soluble ions exhibit not only strong spatial dependence but also different ratios to each other. Asbestos fiber (crocidolite and amosite) concentration values changed in the range of 2.5 to 14.4 f per liter of air. The number of pollen grains showed that Cedar ranked higher in concentration than other types of pollen, with the maximum concentration at site A.

• 환경영향평가
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• 제16권5호
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• pp.327-340
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• 2007

The $PM_{10}$ and $PM_{2.5}$ aerosols were collected at Busan from March to May, 2005, and the concentrations of some metallic elements were analysed to study their characteristics. The mean concentration of $PM_{10}$ was $66.5{\pm}23.0{\mu}g/m^3$ with a range of 22.2 to $118.1{\mu}g/m^3$. The mean concentration of $PM_{2.5}$ was $46.1{\pm}17.2{\mu}g/m^3$ with a range of 9.7 to $83.3{\mu}g/m^3$. The ratio of $PM_{2.5}/PM_{10}$ was 0.69 at Busan. The distribution of metallic elements for $PM_{10}$ and $PM_{2.5}$ were Cd${\ldots}$ ${\ldots}$ $PM_{10}$ were $94.9{\mu}g/m^3$ and $63.7{\mu}g/m^3$, respectively. And The mean mass concentrations of Asian dust and non Asian dust in $PM_{2.5}$ were $56.9{\mu}g/m^3$ and $45.1{\mu}g/m^3$, respectively. The mean values of crustal enrichment factors for five elements (Cd, Cu, Pb, V and Zn) were all higher than 10, possibly suggesting the influence of anthropogenic sources. The soil contribution ratios for $PM_{10}$ and $PM_{2.5}$ were 20.5% and 19.4, respectively.

• 한국환경보건학회지
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• 제33권5호
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• pp.408-421
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• 2007

The concentration characteristics of atmospheric particle matters (PM) including $PM_{2.5},\;PM_{10}$, and TSP were evaluated through the measurement data of PM_{2.5}$ (fine particulate), PM_{10-2.5}$ (coarse particulate), and PM_{over-10}$ collected using a MCI (multi-nozzle cascade impactor) sampler of a three-stage filter pack in spring of 2006 in Iksan area. During the sampling period of 10-15 March and 24 days from 8 April to 2 May, 32 samples for PM of each size fractions were collected, and then measured for PM mass concentrations and water-soluble inorganic ion species. Average concentrations of $PM_{2.5},\;PM_{10}$, TSP were $57.9{\pm}44.1mg/m^3$, $96.6{\pm}89.1mg/m^3$, and $114.8{\pm}99.7mg/m^3$, respectively. Water-soluble inorganic ion fractions to PM mass were found to be 36.5%, 18.0%, and 11.1% for $PM_{2.5}$, $PM_{10-2.5}$ and $PM_{over-10}$, respectively. By showing the high concentrations of PM samples during Asian dust events, those three fractions of PM were distinguished between the samples of Asian dust event and the samples of no event. However, the increase of PM concentrations observed during Asian dust events showed a different pattern for some Asian dust events. The differences of those three fractions in the size distribution may depend on differences on place of occurrence of Asian dust storm and course of transport from China continent to Iksan area in Korea. However, the extent of PM mass contribution during Asian dust events was generally dominated by the coarse particles rather than the fine fraction of PM. The variations of water-soluble inorganic ion species concentration in those three PM fractions between the samples of Asian dust event and the samples of no event were also discussed in this study.

• Journal of Preventive Medicine and Public Health
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• 제20권2호
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• pp.261-269
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• 1987

In order to investigate working conditions of underground coal mines, this work was undertaken to evaluate the respirable dust and the concentration of quartz in Taeback and Kangneung areas. The concentration of quartz was determined by Fourier Transform Infrared Spectrophotometry. The results were as follows; 1) The concentration of respirable dust of drilling and coal face in Taeback and Kangneung areas were as followed; Arithmetic $Mean{\pm}S.D.(mg/m^3)$ Taeback Drilling: $2.00{\pm}1.56$ Taeback Coal Face: $3.74{\pm}3.14$ Kangneung Drilling: $4.55{\pm}4.51$ Kangneung Coal Face: $5.77{\pm}4.53$ Geometric $Mean{\pm}S.D.(mg/m^3)$ Taeback Drilling: $1.34{\pm}2.81$ Taeback Coal Face : $2.55{\pm}2.61$ Kangneung Drilling : $2.44{\pm}3.63$ Kangneung Coal Face: $4.24{\pm}2.37$ 2) Distribution of respirable dust was well fitted to the log-normal distribution and geometric mean value was $log^{-1}\;0.37{\pm}log^{-1}\;0.47(2.34{\pm}2.95)mg/m^3$. 3) The difference of respirable dust concentrations in Taeback and Kangneung areas was not significant statistically (p>0.05). 4) The concentration of quartz of drilling and coal face in Taeback and Kangneung areas were as followed; Arithmetic $Mean{\pm}S.D.(%)$ Taeback Drilling: $6.18{\pm}5.52$ Taeback Coal Face: $1.89{\pm}1.54$ Kangneung Drilling: $3.54{\pm}2.12$ Kangneung Coal Face: $2.05{\pm}3.37$ Geometric $Mean{\pm}S.D.(%)$ Taeback Drilling: $4.24{\pm}2.59$ Coal Face: $1.39{\pm}2.22$ Kangneung Drilling : $2.55{\pm}3.08$ Kangneung Coal Face : $1.24{\pm}2.33$ 5) Distribution of quartz concentrations was well fitted to the log-normal distribution and geometric mean value was $log^{-1}\;0.33{\pm}log^{-1}\;0.45(2.14{\pm}2.82)%$. 6) The difference of quartz concentrations in Taeback and Kangneung areas was not significant (p>0.05), but significant at drilling sites and coal faces (p<0.05).

• 한국산업보건학회지
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• 제30권2호
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• pp.163-173
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• 2020

Objectives: The study was evaluated exposure variation and daily absorption level of cadmium, lead concentration of ambient air of monthly data from 1999 to 2017 for main exposure factor in outdoor workers. Methods: Based on the monthly data from 'The annual report of air quality in Korea from 1999 to 2018' in 'Air Korea' website in the Korean Ministry of Environment. The monthly data of PM_2.5, PM₁₀, cadmium, lead concentration of ambient air were recalculated to average, minimum, and maximum. And these data were combined to Asian-dust exposure data from 'The annual report of Asian-dust·smog in 2017' of National Institute of Meteorological Sciences in Korea. Results: Geometric mean(minimum-maximum) concentration in ambient air of monthly data were 0.0017 (ND-0.2015) mg/㎥ in cadmium and 0.0467(ND-0.8554) mg/㎥ in Pb from 1999 to 2017. Both of Cd and Pb concentration in ambient air showed the highest concentration in January and the lowest in August among annual variation from 1999 to 2017. PM₁₀ and PM_2.5 level showed the highest in March(PM₁₀) and February (PM_2.5) the lowest in August both of PM₁₀ and PM_2.5. Discussion: Based on exposure data and prior reports, daily Cd absorption was estimated to 0.013(ND-1.511) mg/day from respiration and 1.89 mg/day from daily food(25.2 mg/day of daily Cd intake). In case of Pb, daily absorption was estimated to 0.350(ND-6.416) mg/day from respiration and 1.38-1.71 mg/day from daily food intake. Conclusion: Cd and Pb with Asian-dust have high influential factor to increase the Cd and Pb exposure at Winter and Spring season in outdoor workers.