• Korean Journal of Animal Reproduction
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• v.20 no.2
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• pp.191-196
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• 1996

The objective of this study were to investigate the effects of culture media and additives on the development of bovine in vitro matured(IVM) and in vitro fertilized(IVF) oocytes. In experiment 1, bovine oocytes were cultured in droplets of TC 199 supplemented with 10% fetal bovine serum(FBS) with or without hormones (5$\mu\textrm{g}$/ml FSH, 5$\mu\textrm{g}$/ml LH, 1$\mu\textrm{g}$/ml E2). Cleavage rates of embryos cultured for 40~44hrs after IVF were higher when embryos were cultured in TC 199 supplemented hormones (68.1%, 921/35) than without hormones (52.7%, 77/146), but the percentages of development beyond morulae stage were not difference (20.7%, 19.4%). In experiment 2, the effects of various media such as TC 199, synthetic oviduct fluid(SOF), CR1aa with different energy source (fatal bovine serum, FBS; bovine serum albumin, BSA) on developmental capacity of IVM/IVF bovine embryos were investigated. The developmental rates into morulae and blastocysts were 27.1, 10.7, 6.3 and 0%, respecitvely, in CR1aa plus 3mg/ml BSA, SOF plus 10% FBS, TC 199 plus 10% FBS, SOF plus 3mg/ml BSA. In experiment 3, the comparisons of bovine embryos developed to morulae and blastocysts in different culture media (TC 199, SOF, CR1aa, Menezo's B2) were investigated. The developmental capacity beyond morulae stage were 32.9, 26.6, 11.1 and 7.1%, respectively, in Menezo's B2 plus BSA, CR1aa plus BSA, SOF plus BSA, TC 199 plus FBS medium. The cell numbers of the blastocyst were not different in different cultrue media. In experiment 4, bovine embryos were co-cultured with vobine oviduct epithelial cells(BOEC) in TC 199 plus FBS, SOF plus BSA, CR1aa plus BSA, Menezo's B2 plus BSA. The morula and blastocyst rates were 44.7, 32.9, 26.0 and 23.3%, respectively, in CR1aa TC 199, SOF, and Menezo's B2 medium. The cell numbers of the blastocyst were similar to those of blastocyst developed in different culture media.

• Proceedings of the Korean Nuclear Society Conference
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• 2002.10a
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• pp.123.1-123
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• 2002

• Proceedings of the Korean Nuclear Society Conference
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• 2002.10a
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• pp.237-237
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• 2002

• Molecules and Cells
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• v.38 no.6
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• pp.580-586
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• 2015

While increasing evidence indicates the important function of histone methylation during development, how this process influences cardiac development in vertebrates has not been explored. Here, we elucidate the functions of two histone H3 lysine 4 (H3K4) methylation enzymes, SMYD3 and SETD7, during zebrafish heart morphogenesis using gene expression profiling by whole mount in situ hybridization and antisense morpholino oligonucleotide (MO)-based gene knockdown. We find both smyd3 and setd7 are highly expressed within developing zebrafish heart and knock-down of these genes led to severe defects in cardiac morphogenesis without altering the expressions pattern of heart markers, including cmlc2, vmhc, and amhc. Furthermore, double knock-down by coinjection of smyd3 and setd7 MOs caused the synergistic defects in heart development. As similar to knock-down effect, overexpression of these genes also caused the heart morphogenesis defect in zebrafish. These results indicate that histone modifying enzymes, SMYD3 and SETD7, appear to function synergistically during heart development and their proper functioning is essential for normal heart morphogenesis during development.

• The Journal of the Acoustical Society of Korea
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• v.22 no.5
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• pp.388-402
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• 2003

In this paper, we carried out the word, 4 continuous digits. continuous, and task-independent word recognition experiments to verify the effectiveness of the re-defined phoneme-likely units (PLUs) for the phonetic decision tree based HM-Net (Hidden Markov Network) context-dependent (CD) acoustic modeling in Korean appropriately. In case of the 48 PLUs, the phonemes /ㅂ/, /ㄷ/, /ㄱ/ are separated by initial sound, medial vowel, final consonant, and the consonants /ㄹ/, /ㅈ/, /ㅎ/ are also separated by initial sound, final consonant according to the position of syllable, word, and sentence, respectively. In this paper. therefore, we re-define the 39 PLUs by unifying the one phoneme in the separated initial sound, medial vowel, and final consonant of the 48 PLUs to construct the CD acoustic models effectively. Through the experimental results using the re-defined 39 PLUs, in word recognition experiments with the context-independent (CI) acoustic models, the 48 PLUs has an average of 7.06%, higher recognition accuracy than the 39 PLUs used. But in the speaker-independent word recognition experiments with the CD acoustic models, the 39 PLUs has an average of 0.61% better recognition accuracy than the 48 PLUs used. In the 4 continuous digits recognition experiments with the liaison phenomena. the 39 PLUs has also an average of 6.55% higher recognition accuracy. And then, in continuous speech recognition experiments, the 39 PLUs has an average of 15.08% better recognition accuracy than the 48 PLUs used too. Finally, though the 48, 39 PLUs have the lower recognition accuracy, the 39 PLUs has an average of 1.17% higher recognition characteristic than the 48 PLUs used in the task-independent word recognition experiments according to the unknown contextual factor. Through the above experiments, we verified the effectiveness of the re-defined 39 PLUs compared to the 48PLUs to construct the CD acoustic models in this paper.

• Korean Journal of Microbiology
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• v.55 no.3
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• pp.309-312
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• 2019

Comamonas sp. NLF-7-7 was isolated from biofilter of wastewater treatment plant. The whole-genome sequence of Comamonas sp. NLF-7-7 was analyzed using the PacBio RS II and Illumina HiSeqXten platform. The genome comprises a 3,333,437 bp chromosome with a G + C content of 68.04%, 3,197 total genes, 9 rRNA genes, and 49 tRNA genes. This genome contained pollutants degradation and floc forming genes such as sulfur oxidization pathway (SoxY, SoxZ, SoxA, and SoxB) and floc forming pathway (EpsG, EpsE, EpsF, EpsG, EpsL, and glycosyltransferase), respectively. The Comamonas sp. NLF-7-7 can be used to the purification of wastewater.

• Journal of Nutrition and Health
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• v.19 no.3
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• pp.169-176
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• 1986

The effect of riboflavin feeding on lipid emtabolism in rat were studied. 70 male Sprague-Dawley rats were divided into 7 diet group ; control, riboflavin, cholesterol, MLHP (methyl linoleate hydroperoxide) cholesterol plus MLHP, cholesterol plus riboflavin, cholesterol plus MLHP plus riboflavin. After 4 weeks formular feeding sera of animals were collected, then checked cholesterol, triglyceride, phospholipid levels and lipoprotein by use of cellulose acetate papter. The results were as follows ; 1) Marked increase of triglyceride and phospholipid levels were found in the group feeding with cholesterol, cholesterol plus MLHP. 2) Combined riboflavin feeding prevented the increase of triglyceride and phospholipid levels in the group of cholesterol, cholesterol plus MLPH. 3) Also combined riboflavin feeding prevented the increase of $\beta$-lipoprotein fraction in cholesterol feeding group. Our results demonstrate that riboflavin has an inhibitory effects on the derangements of lipid metabolism due to administration of cholesterol, cholesterol plus MLHP.

• Fashion & Textile Research Journal
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• v.15 no.1
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• pp.35-45
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• 2013

The purposes of this study were to segment the women's plus-size market by the types of store patronage, profile the segments, and examine the differences in satisfaction with clothing attributes and variety of plus-size apparels according to different segments. The questionnaires included the 7-point likert scales on store patronage, satisfaction, clothing involvement and body cathexis. Five body measurements were recorded by sales people, and the respondents also provided information on their weight, height and garment size in addition to demographic characteristics. Questionnaires were collected from 7 franchise stores in Seoul, Anyang, Daegu, and Cheonan during the months of February, 2011 to April, 2011. 210 questionnaires were distributed and 160 were returned. Excluding incomplete questionnaires, a total of 149 questionnaires were used in the final analysis. The cluster analysis based on store patronage identified four segments- major patrons of specialty stores, multi-channel users, regular store users, and internet shopping mall users. Significant differences were found among the four segments of women's plus-size consumers in terms of clothing involvement, age, occupation, education and clothing expenditures. Internet shopping mall user group were in overall less satisfied with several clothing attributes and variety compared to other groups.

• Advances in Traditional Medicine
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• v.7 no.2
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• pp.128-132
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• 2007

Diarun plus, a polyherbal formulation containing herbal ingredients of folkloric Antidiabetic effect, was investigated for its effect on glycemic status in rats and mice. In contrast to conventional chemical induced diabetic animal models, changes in glycemic states were induced by physiological maneuvers. Results revealed that in euglycemic animals Diarun plus elicited little change (-10 to +10%), which was insignificant. In food deprivation/swim exercise induced hypoglycemia, Diarun plus reduced the degree of hypoglycemia in both rats and mice (from 38% to 27% in rats and 45% to 32% in mice). Similarly, the marked hyperglycemia induced by dextrose (70% in rats and 95% in mice) was reduced markedly to 8% and 25% respectively. The findings of the present study suggests that the ingredients of Diarun plus have the unique property of maintaining near euglycemic state irrespective of the altered glycemic state, and that have no significant effect in euglycemic condition.

• Restorative Dentistry and Endodontics
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• v.46 no.1
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• pp.4.1-4.16
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• 2021

Objectives: This study evaluated the biocompatibility and bioactive potential of NeoMTA Plus mixed as a root canal sealer in comparison with MTA Fillapex. Materials and Methods: Polyethylene tubes filled with NeoMTA Plus (n = 20), MTA Fillapex (n = 20), or nothing (control group, CG; n = 20) were inserted into the connective tissue in the dorsal subcutaneous layer of rats. After 7, 15, 30 and 60 days, the specimens were processed for paraffin embedding. The capsule thickness, collagen content, and number of inflammatory cells (ICs) and interleukin-6 (IL-6) immunolabeled cells were measured. von Kossa-positive structures were evaluated and unstained sections were analyzed under polarized light. Two-way analysis of variance was performed, followed by the post hoc Tukey test (p ≤ 0.05). Results: At 7 days, the capsules around NeoMTA Plus and MTA Fillapex had more ICs and IL-6-immunostained cells than the CG. However, at 60 days, there was no significant difference in the IC number between NeoMTA Plus and the CG (p = 0.1137) or the MTA Fillapex group (p = 0.4062), although a greater number of IL-6-immunostained cells was observed in the MTA Fillapex group (p = 0.0353). From 7 to 60 days, the capsule thickness of the NeoMTA Plus and MTA Fillapex specimens significantly decreased, concomitantly with an increase in the collagen content. The capsules around root canal sealers showed positivity to the von Kossa stain and birefringent structures. Conclusions: The NeoMTA Plus root canal sealer is biocompatible and exhibits bioactive potential.