• Title/Summary/Keyword: PHYSIOLOGICAL RESPONSE

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A SERI technique reveals an immunosuppressive activity of a serine-rich protein encoded in Cotesia plutellae bracovirus

  • Barandoc, Karen P.;Park, Jay-Young;Kim, Yong-Gyun
    • BMB Reports
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    • v.43 no.4
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    • pp.279-283
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    • 2010
  • Polydnavirus genome is segmented and dispersed on host wasp chromosome. After replication, the segments form double- stranded circular DNAs and embedded in viral coat proteins. These viral particles are delivered into a parasitized host along with parasitoid eggs. A serine-rich protein (SRP) is predicted in a polydnavirus, Cotesia plutellae bracovirus (CpBV), genome in its segment no. 33 (CpBV-S33), creating CpBV-SRP1. This study explored its expression and physiological function in the diamondback moth, Plutella xylostella, larvae parasitized by C. plutellae. CpBV-SRP1 encodes 122 amino acids with 26 serines and several predicted phosphorylation sites. It is persistently expressed in all tested tissues of parasitized P. xylostella including hemocyte, fat body, and gut. Its physiological function was analyzed by injecting CpBV-S33 and inducing its expression in nonparasitized P. xylostella by a technique called SERI (segment expression and RNA interference). The expression of CpBV-SRP1 significantly impaired the spreading behavior and total cell count of hemocytes of treated larvae. Subsequent RNA interference of CpBV-SRP1 rescued the immunosuppressive response. This study reports the persistent expression of CpBV-SRP1 in a parasitized host and its parasitic role in suppressing the host immune response by altering hemocyte behavior and survival.

Evaluation of Thermal Comfort on Protective Clothing Worn in an Radiation Power Plant

  • Kim, Eun-Joo;Han, Myung-Sook;Lee, Won-Ja
    • The International Journal of Costume Culture
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    • v.3 no.1
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    • pp.30-40
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    • 2000
  • The purpose of this research was to device thermal comfort model for radiation power plant workers in protective clothing. Three fabrics commonly used in protective workwear were made into coveralls of identical design and were evaluated by adult healthy males in four simulated work environment. It was investigated between the physiological response and subjects comfort according to environmental variance and clothing types. The of simulated work enviro mensent was controlled under four different humidity and temperature of each type. (Temperature 20±1℃, RH 40∼70%±5%, Temperature 30±1℃, RH 40∼70%±5%) An index of physiological response was connected with the thermal comfort designed. Mean skin temperature, skim temperature, Axillasy temperature ear canal temperature, clothing climate, total sweat, blood pressure, and R-R interval were be evaluated. Skin temperature difference ocurring during exercise and rest were significant only with respect to time and regions of the body, This despite physical differences in the three coveralls, particulary mass statistically experiment. Also, an index of subject wearing sensation was designed for thermal comfort after investigation determined the kind of clothes and the type of environment. As a result of this research, two types of multiple regressions was deviced to estimate thermal comfort of the protective clothing.

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Pysiological Responses of Diploid and Triploid Far Eastern Catfish, Silurus asotus to Water Temperature Stress

  • Lee, Hyo Bin;Kim, Dong Soo;Gil, Hyun Woo;Park, In-Seok
    • Development and Reproduction
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    • v.22 no.2
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    • pp.165-174
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    • 2018
  • The aim of study is to contribute to this knowledge-base by investigating the respiratory function, the metabolic rate and the difference of physiological responses un-der low water temperature ($20^{\circ}C{\rightarrow}15^{\circ}C$) stress be-tween diploid and triploid far eastern catfish, Silurus asotus. During the 48 hrs of water temperature stress exposure time, the respiratory frequencies, $CO_2$ and ${NH_4}^+$ concentrations of diploid had higher values than those of triploid (p<0.05). However, pH of triploid was higher than those of diploid (p<0.05), and oxygen consumption rate was not different between diploid and triploid (p>0.05). The level of plasma cortisol and plasma glucose of triploid was lower than those of the diploid (p<0.05). However, in case of lactic acid, there were not significant between triploid and diploid (p>0.05). These results suggest that diploid was more sensitive for low water temperature stress response than triploid in this species.

Differences of Psychophysiological Responses due to Individual Sensitivity of Simulator Sickness (Simulator Sickness의 개인적인 민감도에 따른 심리생리학적 반응의 차이)

  • 정순철;민병찬;정은지;이봉수;이정한;김철중
    • Journal of Korean Society of Industrial and Systems Engineering
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    • v.25 no.2
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    • pp.11-18
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    • 2002
  • Psychological and physiological effects of the simulator sickness could be important bias factors for the sensibility evaluation. The purpose of the present study was to clear the differences of psychophysiological responses due to individual sensitivity of simulator sickness. The present experiment tried to investigate the simulator sickness objectively by observing the change of the simulator sickness for the different level of sickness groups (sick and non_sick group). The subjective evaluations using Simulator Sickness Questionnaire (SSQ) and physiological responses were measured every five minutes when they were driving as 60km/h in the driving graphic simulator. Response level of the subjective evaluation for all subjects on the simulator sickness was linearly increased with time for every item, and the response level of sick group was bigger than that of non_sick group. When the analysis on central nervous system was done separately on the sick and the non_sick group, there was significant difference in the parameter $\theta$/total at Fz and Cz. Although the analysis on autonomic nervous system for all subjects showed the increased activation of sympathetic nervous system, there was no significant difference between the sick and non_sick group. In summary, it is necessary to select the subjects who feel less simulator sickness in order to be accurate sensibility evaluation. The parameters to distinguish between the sick and non_sick group were the change on $\theta$/total and subjective evaluation using SSQ.

Experimental Study on Cerebral Hemodynamics during Observation of Plants

  • Suda, Ayumu;Lee, Ju-Young;Fujii, Eijiro
    • Proceedings of the Korean Institute of Landscape Architecture Conference
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    • 2007.10b
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    • pp.214-219
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    • 2007
  • Psychological and physiological effects of plants were studied by investigating human responses while observing plants. Eighteen healthy adult male(aged between $19{\sim}25$ years) participated in this study. Semantic differential method(SD method) and multi-channel near-infrared spectroscopy(NIRS) were used to survey verbal and non-verbal response, respectively. Cerebral hemodynamics as a new evaluation index of brain activity was recorded for right brain hemisphere where visual information is mainly delivered. Thirty seconds of cerebral blood flow in forty seven channels were calculated when watching five types of picture images with different rates of hedge against gray block wall; 0:10, 3:7, 5:5, 7:3, 10:0. In the SD results, similar evaluations were found in all subjects. However, the change of cerebral hemodynamics as a non-verbal response varied among subjects. Largely two patterns of hemodynamics change were found with increasing plants rate in picture images; group A showed significant decreases of blood flow volume in many cortical regions, Group B had significant increase of blood flow volume in the occipital region for the scenes seen comparatively more plant. Our findings on the cerebral hemodynamics may indicate that there are two patterns of brain activity during observation of plants; group A in which brain areas associated with visual information and thinking work simultaneously to the visual stimuli; group B in which brain areas associated only with visual information work.

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Effects of induced heat stress on temperature response and biochemistry: alteration of biochemical constituents in Holstein calves by heat stress

  • Lim, Hyun-Joo;Ki, Kwang-Seok
    • Korean Journal of Agricultural Science
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    • v.46 no.3
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    • pp.637-643
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    • 2019
  • The aim of the present study was to evaluate the effect of the induced heat stress on physiological response and serum biochemical parameters involving glucose, cholesterol, blood urea nitrogen (BUN), non-esterified fatty acids (NEFA), and cortisol in Holstein calves. Ten calves were kept in a climate controlled room (air temperature $37^{\circ}C$ and 90% humidity from 09:00 to 19:00) for three days. Those animals were given a one-day adaptation period. During the treatment period, we measured the skin temperature six times. Following the treatment periods, blood samples were collected before the experiment began (09:00) and at the end of the stress period (19:00). To aid analysis of the biochemical parameters, also we monitored the rectal temperature. The results, exhibited that both rectal and skin temperature showed increase in the heat stress-induced animals as compared with unstressed animals. Moreover, we noticed that the levels of BUN and NEFA increased in the blood serum of heat stress induced animals when compared with un-stressed ones. From these results, we concluded that the physiological and biochemical changes in the calves were induced by heat stress. Hence, the present study findings could be employed as base line data for development of stress reduction techniques in the dairy industry.

Acute and Chronic Effects of Nanoplastics on the Water Flea Moina macrocopa

  • Md. Niamul Haque;Jaehee Kim;Jae-Sung Rhee
    • Journal of Marine Life Science
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    • v.8 no.1
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    • pp.78-86
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    • 2023
  • Here, upon acute (96 h) and chronic (14 days) exposure, ingestion of polystyrene NPs (100 nm) and physiological, biochemical, and cholinergic modulations were analyzed in the water flea Moina macrocopa exposed to different concentrations (0.001, 0.01, 0.1, 1, 5, 10, 50, 100, and 500 ㎍ l-1). Exposed NPs were observed in the internal organs (e.g., digestive tract and foregut) of the water flea. Chronic exposure to the relatively high concentrations resulted in significant decreases in survival, body length, and the total number of molts, whereas reproduction parameter was not affected. Significant increase in oxidative stress biomarker (malondialdehyde) and decrease in the intracellular content of endogenous antioxidant (glutathione) and enzymatic activity of antioxidant enzymes (glutathione peroxidase, glutathione reductase, catalase, superoxide dismutase, and glutathione S-transferase) were detected in response to relatively high concentrations of NPs. Transcriptional expression of the hsp70 gene was increased in response to relatively high concentrations of NPs, whereas acetylcholinesterase activity was lowered by the same concentrations of NPs. Taken together, NPs exposure would be a significant modulator on physiological and biochemical metabolism of water flea.

Lichen as Bioindicators: Assessing their Response to Heavy Metal Pollution in Their Native Ecosystem

  • Jiho Yang;Soon-Ok Oh;Jae-Seoun Hur
    • Mycobiology
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    • v.51 no.5
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    • pp.343-353
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    • 2023
  • Lichens play crucial roles in the ecosystems, contributing to soil formation and nutrient cycling, and being used in biomonitoring efforts to assess the sustainability of ecosystems including air quality. Previous studies on heavy metal accumulation in lichens have mostly relied on manipulated environments, such as transplanted lichens, leaving us with a dearth of research on how lichens physiologically respond to heavy metal exposure in their natural habitats. To fill this knowledge gap, we investigated lichens from two of South Korea's geographically distant regions, Gangwon Province and Jeju Island, and examined whether difference in ambient heavy metal concentrations could be detected through physiological variables, including chlorophyll damage, lipid oxidation, and protein content. The physiological variables of lichens in response to heavy metals differed according to the collection area: Arsenic exerted a significant impact on chlorophyll degradation and protein content. The degree of fatty acid oxidation in lichens was associated with increased Cu concentrations. Our research highlights the value of lichens as a bioindicator, as we found that even small variations in ambient heavy metal concentrations can be detected in natural lichens. Furthermore, our study sheds light on which physiology variables that can be used as indicators of specific heavy metals, underscoring the potential of lichens for future ecology studies.

Effects of Design and Material Change of Firefighter Station Uniform on Thermal Physiological and Subjective Responses (소방 기동복의 디자인과 소재 변화가 착용자의 온열생리 반응 및 주관적 감각에 미치는 영향)

  • Kim, Hee-Eun;Kim, Seong-Suk;Son, Su-Young
    • Journal of the Korean Society of Clothing and Textiles
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    • v.44 no.4
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    • pp.776-787
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    • 2020
  • This study investigated physiological and subjective responses to different types of firefighter station uniforms made with various designs and materials. Six healthy males participated in this study that consisted of 20 min of rest, 30 min of treadmill exercise, and 30 min of recovery in a hot and humid environment (34℃ and 65%RH). The experimental clothing conditions were as follows. 1) a fitted T-shirt and trouser made of 100% polyester (FC-Uniform), and 2) flame retardant T-shirts made of acrylic and cotton as well as trousers with aramid and polyester, designed for overfitting (Control). There were no significant differences in the body temperature, and sweat rate between the two conditions; however, the heart rate with the FC-Uniform was significantly lower than Control (p=.025). The clothing microclimate temperature at the chest of the FC-Uniform was significantly lower than the Control (p=.037), and a difference of 1℃ was maintained until the recovery was complete. There were no significant differences in the subjective responses; however, participants experienced a humidity sensation faster with FC-Uniform in the recovery phase. The results indicate that changes in the design and material of firefighter station uniforms may have a positive influence on reducing the thermal stress of firefighters.

Measurement of Emotional Transition Using Physiological Signals of Audiences (관객의 생체신호 분석을 통한 감성 변화)

  • Kim, Wan-Suk;Ham, Jun-Seok;Sohn, Choong-Yeon;Yun, Jae-Sun;Lim, Chan;Ko, Il-Ju
    • The Journal of the Korea Contents Association
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    • v.10 no.8
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    • pp.168-176
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    • 2010
  • Audience observing visual media with care experience lots of emotional transition according to characteristics of media. Enjoy, sadness, surprising, etc, a variety of emotional state of audiences is often arranged by James Russell's 'A circumplex model of affect' utilized on psychology. Especially, in various emotions, 'Uncanny' mentioned by Sigmund Freud is represented a sharp medium existing in a crack of clearly emotional conception. Uncanny phenomenon is an emotional state of changing from unpleasant to pleasant on an audience observing visual media is been aware of immoral media generally, therefore, because this is a positive state on a social taboo, we need to analyze with a scientific analysis clearly. Therefore, this study will organize James Russell's 'A circumplex model of affect' and uncanny phenomenon, will be progressed to establish a hypothesis about a state of uncanny on audiences observing visual media and analyze results of the physiological signals experiment based on ECG(Electronic Cardiogram), GSR(Galvanic Skin Response) signals with distribution, distance, and moving time in a circumplex model of affect.