• Title/Summary/Keyword: PHOL stain

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Association of Prototheca with Bovine Mastitis (Prototheca에 의한 소 유방염 발생예)

  • 팔마헨드라;이창우
    • Journal of Veterinary Clinics
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    • v.14 no.2
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    • pp.349-351
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    • 1997
  • 7세의 홀스타인 암소에서 엽록소가 없는 Prototheca에 의한 만성적인 진행성 유방 염의 발생에 대해 보고한다. 유방염 우유로 만든 습윤표본에서 명확한 세포벽과 내생포자를 갖고 있는 구형 내지 난원형의 병원체가 직접 관찰되었고, Sabouraud배지에서의 순수배양물 에 대해 Wright염색 및 PHOL염색을 했을 때 algae가 증명되었으며, nutrient배지에서 세균 이 중식하지 않아서 Prototheca가 유방염의 원인으로 제시되었다. 이 병원체는 자연상태에서 는 사물기생성이지만 감염원은 확실하지 않았다. algae와 진균의 형태학적인 연구를 하는데 있어서 PHOL염색액을 광범위하게 응용할 수 있음을 제시하였다. Prototheca의 ecology와 병원성을 규명하기 위해 더 많은 연구가 이루어져야 될 것으로 생각된다.

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Clinical and Mycological Observations on Equine Ringworm Due to Microsporum gypseujm (말에 있어서 Microsporum gypseum에 의한 백선증의 임상학적 및 진균학적 관찰)

  • Pal Mahendra;Matsusaka Naonori;Lee Chang Woo
    • Journal of Veterinary Clinics
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    • v.11 no.1
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    • pp.343-346
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    • 1994
  • Microsporum gypseum was identified as the prime cause of dermatitis in two young horses who were housed in unhygienic stables. The lesions were mainly distributed on the thorax, abdomen and rump. The diagnosis was established on the direct demonstration of dermatophyte in the cutaneous lesions and isolation of the fungus in pure and heavy growth from the infected hairs and skin scales on mycological medium at 3$0^{\circ}C$. Microscopoc morphology of the isolate in 'PHOL' stain many macroconidia and few microconidia. Epidemoilogical investigation revealed the prevalence of M. gypseum in the soil of stables. Mycological examination is highly imperative to distinguish the disease from other dermatological disorders. This apperas to be the first report of equine dermatitis due to M. gypseum in Westenn India.

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Occurrence of Geotrichum candidum in Two Cases of Canine Dermatitis (두 마리 개의 피부염에 있어서 Geotrichum candidum의 존재)

  • Mahendra Pal;Chang woo Lee;Naonori Matsusaka
    • Journal of Veterinary Clinics
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    • v.19 no.4
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    • pp.433-435
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    • 2002
  • Ceotrichum candidum was identified from 2 dogs among 52 dogs which had various dermatological problems. One was 5-month-old male Labrador and the other was 7-month-old female German Shepherd. The diagnosis was confirmed by direct microscopy of the specimen as well as cultured isolation of the fungus from the cutaneous lesions. The. organism showed septate hyphae and rectangular to cylindrical arthrospores and no blastospores. Urea was not utilized and no assimilation of maltose was observed. Clinical improvement was occurred by application of 2% gentian violet and 2% miconazole solutions. It is suggested that this organism should be included in the differential diagnosis of canine dermatitis.