• Health Policy and Management
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• v.23 no.4
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• pp.415-426
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• 2013

Background: This study was aim to identify the specific words and to develop the scale for the public health center (PHC) image. Methods: We collected 824 words from the previous studies and by open questions and reduced them by 77 words, then which were rated properly by 355 citizens of Seoul. We examined explanatory factor analysis for 69 words, and examined content validity test and confirmatory factor analysis (CFA) for the image structures (4 factors and 16 words). And then we developed the image questionnaire using them through council. We conducted a survey and retested the PHC image scales as the measuring tool targeting 2,000 persons, and compared the inexperience and experience persons for PHC usage. Results: The image structures were consisted of 4 factors and 16 words such as 'trustworthiness' (warm, exemplary, faithful, service-mindedness, beneficial to health), 'fairness' (honesty, clear, consistent, ruled), 'development possibility' (changing, goal-directed, developmental, propulsive), and 'flexibility' (not authoritative, not perfunctory, not rigid) in total. Cronbach's ${\alpha}$ values of all factors were above 0.7. As a result of CFA, model fit indexes yielded satisfactory results (root mean square error of approximation [RMSEA] 0.049, goodness of fit index [GFI] 0.937, and adjusted goodness of fit index [AGFI] 0.912). According to the result of retest for measuring tool by using other samples, Cronbach's ${\alpha}$ values were above 0.8, and model fit indexes yielded satisfactory results (RMSEA 0.059, GFI 0.952, AGFI 0.933). RMSEAs of the inexperiences and the experiences were each 0.59, 0.68. Conclusion: A reliable, valid, and generalizable scale was created for PHC image.

• 보존과학연구
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• s.19
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• pp.3-22
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• 1998

The effect of pH on degradation of paper by some fungi, which able to degrade cellulose, was investigated. Trichoderma koningii, Aspergillus nigerand Penicillium nigulosum were cultured at $28^{\circ}C$ for 16 days in the selective medium (PH3, PH4, PH5, PH6, PH7, PH8, PH9, PH10, PHC) containing paper as substrate. Each paper was pretreated with each pH buffer (pH 3∼pH 10, D.W.)prior to addition to the selective medium. Enzyme activities in the each culture medium were measured spectroph to metrically using C.M.C., Avicel, PNPG as the substrates for endoglucanase, exoglucanase and $\beta$-glucosidase, respectively. In all experimental fungi, the enzyme activities of PH3 and PH9 medium were usually much higher than those of other experimental groups. However in the PH6medium, enzyme activity was lower than other groups. To analyze the concentration and pattern of protein in the each culture medium, the medium was concentrated by lyophilization. The protein concentration of PH3 and PH9 medium were relatively high (T.koningii; 6.31mg, 6,19mg, A.niger; 1.62mg, 1.96mg, P.nigulosum;2.50mg, 2.73mg, respectively), but that of PH6 was relatively low. The protein pattern of each medium was analyzed by using SDS-PAGE and VDS Image Master Analysis Program. The concentrations of bands in the each lane were usually high at lane2 (PH3) and lane8 (PH9) and low at lane5 (PH6). Therefore, the incresed cellulolytic activity of fungus against acidified paper could be result of structural change and deterioration of paper caused by being acidified.