• Korean Journal of Microbiology
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• v.42 no.4
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• pp.319-325
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• 2006

[ $\beta$ ]-Glucans (AG) were prepared from Agaricus blazei cultured in the medium fortified with the roots of Pueraria spp. by repeated extraction with hot water, gel filtration chromatography and DEAE ion exchange chromatography. Oligosaccharides (AO) were derived from the hydrolysis of AG by an endo-$\beta$-(1$\rightarrow$6)-glucanase from Bacillus megaterium. The anti-HT-29 human colon cancer activity of AG or AO was investigated using MTT assay, apoptosis assay, cell cycle analysis, and cDNA microairay. AG and AO both inhibited proliferation and growth of HT-29 cells, and stimulated apoptosis of the cells in a dose-dependent manner. In cell cycle analysis, treating HT-29 cells with AG or AO resulted in the increase of cells in the G0 (sub-G1) and G1 phase. Especially, AO was more effective in inducing G0/G1 cell cycle arrest than AG. To screen the genes involved in the increase of apoptosis, the gene expression profile of the HT-29 cells treated with AO was examined by cDNA microarray. While several genes involved in cell cycle progression (CCND2 and CDK2) were down-regulated, many genes involved in apoptosis (TNFSF9, TNFRSF9, FADD, CASP8, BAD, CRADD, CASP9 etc), cell cycle inhibitor (CDKN2A), immune response (IL6, IL18, IL6R etc), and tumor suppressor (CEACAM1, TP53BP2, IRF1, and PHB) were up-regulated. These results suggest that AO could inhibit the proliferation and growth of HT-29 cells by G0/G1 cell cycle arrest and induction of apoptosis.

• Korean Journal of Microbiology
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• v.53 no.3
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• pp.200-207
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• 2017

A new approach to the solubilization of waste activated sludge (WAS) using alginate-quaternary ammonium complex beads was investigated under controlled mild alkaline conditions. The complex beads were prepared by the reaction of sodium alginate (SA) with 3-(trimethoxysilyl)propyl-octadecyldimethylammonium chloride (TSA) in acid solution, followed by crosslinking with $CaCl_2$. Treatment of WAS with SA-TSA complex beads was effective for enhancing the efficacy of WAS solubilization. The highest value of soluble chemical oxygen demand (SCOD) concentration (3,900 mg/L) was achieved after 10 days of treatment with 30% (v/v) SA-TSA complex beads. The WAS solubilization efficacy of the complex beads was also evaluated by estimating the concentrations of volatile fatty acids (VFAs). The maximum value of VFAs was 2,961 mg/L, and the overall proportions of VFAs were more than 75% of SCOD. The main components of VFAs were acetic, propionic, iso-butyric, and butyric acids. These results suggest that SA-TSA complex beads might be useful for enhancing the solubilization of WAS. The potential use of VFAs as the external carbon substrate for the production of polyhydroxyalkanoate (PHA) by a mixed microbial culture (MMC) was also examined. The enrichment of PHA-accumulating MMC could be achieved by periodic feeding of VFAs generated from WAS in a sequencing batch reactor. The composition of PHA synthesized from VFAs mainly consisted of 3-hydroxybutyrate. The maximum PHA content accounted for 25.9% of dry cell weight. PHA production by this process is considered to be promising since it has a doubly beneficial effect on the environment by reducing the amount of WAS and concomitantly producing an eco-friendly biopolymer.

• Journal of the Institute of Electronics Engineers of Korea TC
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• v.39 no.5
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• pp.232-242
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• 2002

In a MPLS(Multiprotocol Label Switching) network, it is important to reduce the number of labels and LSP(Lable Switched Path)s for network resource management. MTP(Multipoint-to-Point) LSP can be used to solve this problem. In consideration of traffic engineering, MTP LSP must be chosen to enhance the availability of network and link utilization. Also, a fast mechanism to setup MTP LSPs is required for rerouting capability against link failure. In this paper, we propose a fast MTP LSP traffic engineering of multipath MTP LSP by using a mapping of a MTP LSP upon Diffserv PHBs(Per Hop Behavior) in a Diffserv-capable MPLS network. In the proposed traffic engineering, we determine multiple MTP LSPs in a hierarchical manner according to the characteristics of different services. By using Monte-Carlo method for traffic load balancing process, it provides fast rerouting capability in case of frequent link failure across large network. Out method produces to be nearly optimal within reasonable run-times. It's time complexity is in O( Cn$^2$logn) as conventional multipath routing and it is much faster than Linear Programming approach. Simulation results show that the proposed traffic engineering can be controlled effectively in an administrative manner and enhance the availability of network in comparison with conventional multipath routing.

• Journal of the Institute of Electronics Engineers of Korea TC
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• v.42 no.12
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• pp.149-158
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• 2005

This paper proposes a congestion avoidance algorithm which provides stable throughput and transmission rate regardless of buffer size by limiting the TCP congestion window in fixed bandwidth networks. Additive Increase, Multiplicative Decrease (AIMD) is the most commonly used congestion control algorithm. But, the AIMD-based TCP congestion control method causes unnecessary packet losses and retransmissions from the congestion window increment for available bandwidth verification when used in fixed bandwidth networks. In addition, the saw tooth variation of TCP throughput is inappropriate to be adopted for the applications that require low bandwidth variation. We present an algorithm in which congestion window can be limited under appropriate circumstances to avoid congestion losses while still addressing fairness issues. The maximum congestion window is determined from delay information to avoid queueing at the bottleneck node, hence stabilizes the throughput and the transmission rate of the connection without buffer and window control process. Simulations have performed to verify compatibility, steady state throughput, steady state packet loss count, and the variance of congestion window. The proposed algorithm can be easily adopted to the sender and is easy to deploy avoiding changes in network routers and user programs. The proposed algorithm can be applied to enhance the performance of the high-speed access network which is one of the fixed bandwidth networks.

• Journal of Life Science
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• v.32 no.7
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• pp.510-522
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• 2022

In this study, to biosynthesize PHA with properties more similar to polypropylene, a Bacillus sp. EMK-5020 strain that biosynthesized poly (3-hydroxybutyrate-co-3-hydroxyvalerate) (PHBV) was isolated from soil. Bacillus sp. EMK-5020 strain biosynthesized PHBV containing 1.3% 3-hydroxyvalerate (3HV) using reducing sugar contained in Makgeolli lees enzymatic hydrolysate (MLEH) as a single carbon source. As the amount of propionic acid, which was added as a second carbon source, increased, the content of 3HV also increased. PHBV containing up to 48.6% of 3HV was synthesized when 1.0 g/l of propionic acid was added. Based on these results, the strain was cultured for 72 hr in a 3 l fermenter using reducing sugar in MLEH (20 g/l) and propionic acid (1 g/l) as the main and secondary carbon sources, respectively. As a result, 6.4 g/l DCW and 50 wt% of PHBV (MLEH-PHBV) containing 8.9% 3HV were biosynthesized. Through gel permeation chromatography and thermogravimetric analysis, it was confirmed that the average molecular weight and the decomposition temperature of MLEH-PHBV were 152 kDa and 273℃, respectively. In conclusion, the Bacillus sp. EMK-5020 strain could biosynthesize PHBV containing various 3HV fractions when MLEH and propionic acid were used as carbon sources, and PHBV-MLEH containing 8.9% 3HV was confirmed to have higher thermal stability than standard PHBV (8% 3HV).