• Journal of Microbiology and Biotechnology
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• v.33 no.4
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• pp.485-492
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• 2023

Methylorubrum extorquens, a facultative methylotroph, assimilates C₁ compounds and accumulates poly-β-hydroxylbutyrate (PHB) as carbon and energy sources. The ethylmalonyl pathway is central to the carbon metabolism of M. extorquens, and is linked with a serine cycle and a PHB biosynthesis pathway. Understanding the ethylmalonyl pathway is vital in utilizing methylotrophs to produce value-added chemicals. In this study, we determined the crystal structure of the mesaconyl-CoA hydratase from M. extorquens (MeMeaC) that catalyzes the reversible conversion of mesaconyl-CoA to β-methylmalyl-CoA. The crystal structure of MeMeaC revealed that the enzyme belongs to the MaoC-like dehydratase domain superfamily and functions as a trimer. In our current MeMeaC structure, malic acid occupied the substrate binding site, which reveals how MeMeaC recognizes the β-methylmalyl-moiety of its substrate. The active site of the enzyme was further speculated by comparing its structure with those of other MaoC-like hydratases.

• Microbiology and Biotechnology Letters
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• v.32 no.3
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• pp.243-248
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• 2004

Production of (R)-3-hydroxybutyric acid (R3HB) by fed-batch culture and continuous culture of metabolically engineered Escherichia coli harboring Ralstonia eutropha PHB biosynthesis and depolymerase genes was examined in a 30 1 pilot-scale fermentor. A new stable two-plasmid system, pBRRed containing the R. eutropha PHB depolymerase gene and pMCS 105 containing the R. eutropha PHB biosynthesis genes, was developed. Among a variety of E. coli strains harboring plasmids, recombinant E. coli XL-10 Gold (pBRRed, pMCS105) was able to produce R3HB with the highest efficiency in a batch culture. By the fed-batch culture of recombinant E. coli XL-10 Gold(pBRRed, pMCS 105) in a 30 1 fer-mentor, the final R3HB concentration was 22.4 g/l giving a productivity of 0.97 g/l-h. To produce R3HB to a high concentration with high productivity, a new strategy of fed-batch culture followed by a continuous culture was investigated. The maximum productivity and R3HB concentration were 5.06 g/l-h and 25.3 g/l, respectively. These results show that economical production of R3HB is possible by recombinant E. coli in large scale.

• Proceedings of the Korean Fiber Society Conference
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• 1998.04a
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• pp.20-24
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• 1998

Poly(ethylene 2,6-naphthalate) (PEN) has been known since 1948, when its synthesis was first reported by ICI. Co. In spite of its long history. application of PEN is limited as compared with poly(ethylene terephthalate) (PET). because PEN monomer is very expensive, and PEN exhibits relatively high melt viscosity that is not easy for fiber spinning and injection molding.(omitted)

• Proceedings of the Korean Information Science Society Conference
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• 2003.10c
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• pp.127-129
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• 2003

현재의 인터넷은 트래픽의 양적 증가뿐 아니라 다양한 응용 수용이라는 질적인 측면의 문제에 봉착했다. 이를 수용하기 위해서 Diffserv 개념이 도입되었으며, 본 논문에서는 프로그램 가능한 네트워크 프로세서 IXP1200를 이용하여 간단한 Diffserv Interior­node PHB의 설계를 통하여 CoS(Class of Service)를 지원하는 Diffserv를 설계하고 구현하였다.

• Proceedings of the Korean Fiber Society Conference
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• 2001.10a
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• pp.199-202
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• 2001

현재 플라스틱에 의한 환경오염이 사회문제로까지 확대되면서 이를 해결하기 위한 한가지 대책으로 분해성 플라스틱의 개발이 절실히 요구되어 전세계적으로 많은 연구가 이루어지고 있다. 생분해성을 가지는 미생물 폴리에스테르 중에서 가장 대표적인 poly(3-hydroxybutyrate), PHB는 우수한 역학적 성질을 가지며, 또한 천연고분자 중에서 융점을 가지는 유일한 열가소성 재료이다. (중략)

• Biotechnology and Bioprocess Engineering:BBE
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• v.7 no.5
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• pp.295-302
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• 2002

To understand the utilization property of light energy, Synechococcus sp. MA19, a poly-${\beta}$-hydroxybutyrate (PHB) producer, was cultivated at the different incident light intensities of 15.3, 50.0 and 78.2 W/$m^2$ using media with and without phosphate. From the results of metabolic flux analysis, it was found that the cell yield based on ATP synthesis was estimated as $3.5{\times}10^{-3}$ kg-biomass/mol-ATP in these cultures. Under the examined conditions, there were no significant differences in the efficiency of light energy conversion to chemical energies estimated as ATP synthesis and reducing potential (NADH + NADPH) formation whether the PHB synthesis took place or not. The energy converted from light to ATP was kept relatively high around the energy absorbed by the cells of $2.5-3.0{\times}10^{6} J\;h^{-1}\;kg^{-1}$, whereas the energy of reducing potential was hardly changed in the examined range of the energy absorbed by the cells.

• The Journal of Korean Institute of Communications and Information Sciences
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• v.29 no.3B
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• pp.325-338
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• 2004

As the recent rapid development of internet technology and the wide spread of multimedia communication, massive increase of network traffic causes some problems such as the lack of network paths and the bad quality of service. To resolve these problems, this paper presents a traffic control agent that can perform the dynamic resource allocation by controlling traffic flows on a DiffServ network. In addition, this paper presents a router that can support DiffServ on Linux to support selective QoS in IP network environment. To implement a method for selective traffic transmission based on priority on a DiffServ router, this paper changes the queuing discipline in Linux, and presents the traffic control agent so that it can efficiently control routers, efficiently allocates network resources according to service requests, and relocate resources in response to state changes of the network. Particularly for the efficient processing of Assured Forwarding(AF) Per Hop Behavior(PHB), this paper proposes an ACWF$^2$Q$^{+}$ packet scheduler on a DiffServ router to enhance the throughput of packet transmission and the fairness of traffic services.s.

• Journal of the Institute of Electronics Engineers of Korea TC
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• v.40 no.12
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• pp.125-135
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• 2003

In this paper, we developmed and implemented router and bandwidth broker that provides end-to-end QoS in differentiated service IP networks. Our design goal is to provide end-to-end premium service using the EF PHB. The bandwidth broker and router were implemented in the FreeBSD 4.6 platform. For the DiffServ router, we implemented QoS daemon, rollback managment routine, monitoring, and COPS-PR client. Also, Ive implemented bandwith broker which has SLS and resource management function, and signaling protocols for interfacing intra-and inter-domain. On the testbed, we have validated and evaluated the performance of the implemented DiffServ router and bandwidth broker using the inter-domain signaling protocol client in MS Windows platform. The test results show that the end-to-end QoS can be guaranteed for the inter-domain diffServ by the implemented system.

• The Journal of the Korea institute of electronic communication sciences
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• v.11 no.3
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• pp.303-308
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• 2016

Recently, as the rapid development of network technology and the increase of services required high bandwidth such as multimedia service, the network traffic dramatically increases. This massive increase of network traffic causes some problems such as the degradation of QoS and the lack of network resources and, to solve these problems, various research to guarantee QoS have been performing. Currently, The most representative method to guarantee the QoS is the DiffServ(: Differentiated Service). The DiffServ defines the AF(: Assured Forwarding) PHB(: Per Hop Behavior) and statistically ensures the throughput over the certain level of data rate. However, the TCP congestion control method that make up the majority of the Internet traffic is not fundamentally suitable to the DiffServ that guarantees the throughput without managing the individual flow. Therefore, in this paper, we present this mismatch through the simulation as an example and propose the solution by controlling the TCP of the terminal in the network. The proposed scheme utilizes the information of the reception window size included in the ACK frame and does not require any modification of the TCP algorithms currently in use.

• Journal of Microbiology and Biotechnology
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• v.9 no.6
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• pp.847-853
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• 1999

A Pseudomonas sp. strain that is capable of utilizing dicarboxylic acids as a sole carbon source was isolated from activated sludge by using the enrichment culture technique. This organism accumulated polyhydroxyalkanoates (PHAs) with an unusual pattern of monomer units that depends on the carbon sources used. Polyhydroxybutyrate (PHB) homopolyester was synthesized from glucose or small $C_{-even}$ alkanoic acids, such as butyric acid and hexanoic acid. Accumulation of PHB homopolyester was also observed in the cells grown on $C_{-odd}$ dicarboxylic acids, such as heptanedioic acid and nonanedioic acid as the sole carbon sources. In contrast, a copolyester consisting of 6 mol% 3-hydroxybutyrate (3HB) and 94 mol% 3-hydroxyvalerate (3HV) was produced with a PHA content of as much as 36% of the cellular dry matter. This strain produced PHAs consisting both of the short-chain-length (SCL) and the medium-chain-length (MCL) 3-hydroxyacid units when heptanoic acid to undecanoic acid were fed as the sole carbon sources. Most interestingly, polyester consisting of significant amount of relevant fractions, 3HB, 3HV, and 3-hydroxyheptanoate (3HHp), was accumulated from heptanoic acid. According to solvent fractionation experiments, the polymer produced from heptanoic acid was a blend of poly(3HHp) and of a copolyester of 3HB, 3HV, and 3HHp units. The hexane soluble fractions contained only 3HHp units while the hexane-insoluble fractions contained 3HB and 3HV units with a small amount of 3HHp unit. The copolyester was an elastomer with unusual mechanical properties. The maximum elongation ratio of the copolyester was 460% with an ultimate strength of 10 MPa, which was very different from those of poly(3HB-co-3HV) copolyesters having similar compositions produced from other microorganisms.