• Journal of the Korea Institute of Information Security & Cryptology
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• v.17 no.5
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• pp.27-38
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• 2007

One of the most important security issues of e-mail service is user privacy. Currently, various security protocols, like PGP(pretty Good Privacy), S/MIME(Secure/Multipurpose Internet Mail Extension), have been proposed. These protocols, however, do not provide forward secrecy. Recently, some security protocols that provide forward secrecy were proposed. But all of them require changes to the current e-mail infrastructure. Moreover, contrary to authors' intention, some of them do not actually provide perfect forward secrecy. In this paper, we propose a new practical e-mail security protocol. The proposed protocol provides perfect forward secrecy and uses a practical e-mail model that dose not require any changes to existing e-mail servers. It encrypts and authenticates messages efficiently using elliptic curve based signcryption scheme. In addition, we provide a way to send secure group e-mails.

• Journal of Microbiology and Biotechnology
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• v.33 no.5
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• pp.607-620
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• 2023

The biocontrol approach using beneficial microorganisms to control crop diseases is becoming an essential alternative to chemical fungicides. Therefore, new and efficient biocontrol agents (BCA) are needed. In this study, a rhizospheric actinomycete isolate showed unique and promising antagonistic activity against three of the most common phytopathogenic fungi, Fusarium oxysporum MH105, Rhizoctonia solani To18, and Alternaria brassicicola CBS107. Identification of the antagonistic strain, which was performed according to spore morphology and cell wall chemotype, suggested that it belongs to the Nocardiopsaceae. Furthermore, cultural, physiological, and biochemical characteristics, together with phylogenetic analysis of the 16S rRNA gene (OP869859.1), indicated the identity of this strain to Nocardiopsis alba. The cell-free filtrate (CFF) of the strain was evaluated for its antifungal potency, and the resultant inhibition zone diameters ranged from 17.0 ± 0.92 to 19.5 ± 0.28 mm for the tested fungal species. Additionally, the CFF was evaluated in vitro to control Fusarium wilt disease in Vicia faba using the spraying method under greenhouse conditions, and the results showed marked differences in virulence between the control and treatment plants, indicating the biocontrol efficacy of this actinomycete. A promising plant-growth promoting (PGP) ability in seed germination and seedling growth of V. faba was also recorded in vitro for the CFF, which displayed PGP traits of phosphate solubilization (48 mg/100 ml) as well as production of indole acetic acid (34 ㎍/ml) and ammonia (20 ㎍/ml). This study provided scientific validation that the new rhizobacterium Nocardiopsis alba strain BH35 could be further utilized in bioformulation and possesses biocontrol and plant growth-promoting capabilities.

• Nuclear Medicine and Molecular Imaging
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• v.41 no.3
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• pp.209-217
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• 2007

Purpose: Multidrug resistance (MDR) of the cancer cells related to mdr1 gene expression can be effectively treated by selective short hairpin RNA for mdr1 gene (shMDR). Sodium/iodide symporter (NIS) gene is well known to have both reporter and therapeutic gene characteristics. We have co-transfected both shMDR and NIS gene into colon cancer cells (HCT15 cell) expressing MDR and Tc-99m sestamibi and I-125 uptake were measured. In addition, cytotoxic effects of doxorubicin and I-131 therapy were also assessed after transfection. Material and Methods: At first, shMDR was transfected with liposome reagent into human embryonic kidney cells (HEK293) and HCT cells. shMDR transfection was confirmed by RT-PCR and western blot analysis. Adenovirus expressing NIS (Ad-NIS) gene and shMDR (Ad-shMDR) were co-transfected with Ad-NIS into HCT15 cells. Forty-eight hours after infection, inhibition of P-gycoprotein (Pgp) function by shMDR was analyzed by a change of Tc-99m sestamibi uptake and doxorubicin cytotoxicity, and functional activity of induced NIS gene expression was assessed with I-125 uptake assay. Results: In HEK293 cells transfected with shMDR, mdr1 mRNA and Pgp protein expressions were down regulated. HCT15 cells infected with 20 MOI of Ad-NIS was higher NIS protein expression than control cells. After transfection of 300 MOI of Ad-shMDR either with or without 10 MOI of Ad-NIS, uptake of Tc-99m sestamibi increased up to 1.5-fold than control cells. HCT15 cells infected with 10 MOI of Ad-NIS showed approximately 25-fold higher I-125 uptake than control cells. Cotransfection of Ad-shMDR and Ad-NIS resulted in enhanced cytotoxic by doxorubicin in HCT15 cells. I-131 treatment on HCT15 cells infected with 20 MOI of Ad-NIS revealed increased cytotoxic effect. Conclusion: Suppression of mdr1 gene expression, retention of Tc-99m sestamibi, enhanced doxorubicin cytotoxicity and increases in I-125 uptake were achieved in MDR expressing cancer cell by co-transfection of shMDR and NIS gene. Dual therapy with doxorubicin and radioiodine after cotransfection shMDR and NIS gene can be used to overcome MDR.

• Proceedings of the Korean Society of Surveying, Geodesy, Photogrammetry, and Cartography Conference
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• 2004.04a
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• pp.3-8
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• 2004

This study describes the validation of the GPS/Levelling using GPS data that were obtained from three Permanent GPS stations of the Jeju island. Each orthometric height of three Permanent GPS stations was calculated from the Bench mark of levelling with Traverse-survey and relative baseline processing The test result shows that the error of closure of orthometric height was about 1.6cm and one of ellipsoidal height was about 1.5cm. This result represents that the geoidal height and undulations are regular in the Jeju island. According to the test results, it is identified that one can calculate the orthometric height using Permanent GPS station data rather than traditional levelling method which has a some complexity especially in Jeju island.

• Proceedings of the Korean Information Science Society Conference
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• 1998.10a
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• pp.506-508
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• 1998

전세계가 정보화 열풍을 겪고 있는 시점에서, 엄청난 사용자들이 다양한 인터넷 서비스 중 전자우편 서비스를 생활의 일부분으로 사용하고 있다. 그러나 기존의 전자우편 서비스만을 제공하는 환경하에서는 많은 보안 위험요소 들이 존재하고 있으며 전자우편 서비스의 사용에 많은 제약과 노출 위험 가능성을 안고 있다. 이러한 문제점들을 극복하기 위한 노력들이 이미 기울여져 다양한 방법으로 해결책을 제시하고 있지만 아직 완전한 형태의 결과물이 나오지 못하고 있다. 그 중 대표적인 PEM은 구현의 어려움으로 인하여 보편화되지 못하고 있으며, 또 다른 제품의 PGP는 키 인증 등의 문제점을 안고 있는 실정이다. 또한 이들 제품들은 전자우편 보안 요구사항들을 모두 충족시켜주지 못한다는 문제점들도 안고 있다. 본 논문에서는 위에서 언급했던 제품들이 안고 있는 문제점들을 극복할 수 있는 다른 해결책을 내 놓기 위해 모든 보안 요구사항을 만족할 수 있고 사용하기 편리한 전자우편 보안 프로토콜을 설계하였다. 이를 위해서 비대칭키 방식인 키 분배서버를 이용하며, 하부 사용자는 키 분배 서버에 의존해 기밀성 및 무결성등 정보보호 서비스를 제공 받을 수 있다.

• Proceedings of the Korea Institutes of Information Security and Cryptology Conference
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• 2006.06a
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• pp.344-348
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• 2006

현재 PGP(Pretty Good Privacy)와 S/MIME(Secure/Multipurpose Internet Mail Extension)와 같은 여러 가지 이메일 보안 프로토콜들이 제안되어 사용되고 있으나 이들 프로토콜은 최근 중요시되고 있는 보안 요구사항인 전방향 안전성을 보장하지 못한다. 최근에 이 요구사항을 충족하는 이메일 보안 프로토콜들이 제안되었으나 현실적이지 못한 가정 하에 설계되었거나 효율성 측면에서 개선이 필요한 프로토콜들이다. 또한 일부 프로토콜들은 실제 완전한 전방향 안전성을 제공하지 못하고 있다. 이 논문에서는 이 부분을 개선하고, 완전한 전방향 안전성을 제공하는 안전한 이메일 프로토콜을 제안한다. 제안되는 프로토콜은 Zheng 의 signcryption 기법을 사용하여 효율적이고 안전한 인증을 제공한다.

• Proceedings of the Korea Institutes of Information Security and Cryptology Conference
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• 1998.12a
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• pp.21-32
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• 1998

공개키 암호시스템은 온라인 전자상거래와 개방형 네트워크에서 정보보호가 요구되는 많은 응용들에 사용되고 있다. 공개키 암호시스템을 광범위하게 사용하려면 공개키 값을 관리하는 공개키 기반구조(Public Key Infrastructure)가 필요하다 이러한 공개키 기반구조의 기능이 없이, 공개키 암호화는 단지 전통적인 비밀키 암호화와 다를 것이 없다 본 논문은 전자상거래의 기본이 되는 공개키 기반구조가 기본적으로 갖추어야 할 특징과 공개키 기반구조를 구성하는 객체들, 그리고 공개키 기반구조를 구성하는 기본적인 방법에 대해 살펴보았다. 그리고, 현재 인증 기반 시스템으로 제안ㆍ사용되고 있는 PKI 중에서, X.509 기반 시스템(X.509, PEM)과 비-X.509 기반 시스템(PGP, SDSI, SPKI)에 대해 연구를 하였으며, 이에 대해 비교 분석하였다.

• Asian Pacific Journal of Cancer Prevention
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• v.15 no.16
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• pp.6849-6853
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• 2014

Multidrug resistance (MDR) is a major impediment to successful chemotherapy of gastric cancer. Our aim was to establish an epirubicin-resistant cell subline (AGS/EPI) and to elucidate the mechanisms involved in acquired EPI resistance. The AGS/EPI cell subline developed by exposing parental AGS cells to stepwise increasing concentrations of EPI demonstrated 2.52-fold resistance relative to the AGS cell line, and mRNA expression of the ATP-dependent drug-efflux pump P-glycoprotein (Pgp), more recently known as ABCB1 protein, was similarly upregulated. An AGS/EPI cell subline could thus be effectively established, and MDR mechanism of these cells was shown to be related to the overexpression of mRNA of the ABCB1 gene.

• Journal of Applied Biological Chemistry
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• v.48 no.2
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• pp.79-83
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• 2005

While screening for nematicidal activity of bacterial origins, various pseudomonads strains were inhabited in tomato rhizosphere. One isolate designated as $PE_{10}$ was selected for studies on nematicidal properties and plant growth-promoting (PGP) activity and was identified as Pseudomonas aeruginosa based on morphological features, biochemical and physiological tests, and carbohydrate utilization. To investigate nematicidal activity, Meloidogyne incognita juvenile mortality was determined using $PE_{10}$ culture filtrate. Inhibition of strain $PE_{10}$ against Fusarium oxysporum was observed using dual culture technique. Strain $PE_{10}$ showed good siderophore activity, HCN and IAA production abilities, and growth and development enhancement of tomato.

• Annals of Clinical Neurophysiology
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• v.17 no.2
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• pp.53-60
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• 2015

Skin biopsy with investigation of small nerve fiber in human epidermis and dermis has been proven to be a useful method for demonstration of small fiber neuropathy. Quantification of intraepidermal nerve fiber density using anti-Protein Gene Product 9.5 (PGP 9.5) antibody is standardized method to diagnose the small fiber neuropathy. Skin biopsy method also makes it possible to differentiate the type of nerve fibers by using different antibodies. Quantification of dermal structures with different type of nerve fibers could be used to invest pathophysiologic mechanism of diseased state.