• Korean Journal of Soil Science and Fertilizer
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• v.40 no.2
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• pp.145-150
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• 2007

This study was conducted to evaluate the application effects of phospho-gypsum fertilizer (PGF) as reclamation material in the newly reclaimed paddy fields located in Goheung and Youngam, Jeonnam province in Korea. The PGF used in this experiment was produced by Namhae Chemical Co. as the name of Soil-Saver$^{TM}$. Prior to rice transplanting, the PGF was applied as soil amendment as the amount of $3,000kg\;ha^{-1}$. The PGF increases rice plant height and number of tiller at the heading stage by 119.9 cm and 9.1, respectively, in Youngam area. The harvest index of brown rice increased up to 5 and 13% more in the PGF applied paddy field from both sites than in the non-application of PGF at paddy field before rice transplanting, and the ripening ratio in increased in both sites to 81 and 90%. Protein content of brown rice was also greater than in the non-application of PGF at the both sites. For the effects of the reclamation by PGF in the paddy field soils, we found that PGF reduced exchangeable Na to 18 and 28% for both sites, respectively, and increased exchangeable Ca and $SO_4$. And we found relatively higher amounts of $K_2O$, CaO and MgO in the rice plants from both sites applied with PGF.

• Journal of Environmental Health Sciences
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• v.36 no.1
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• pp.44-51
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• 2010

This study aimed to develop analytical method for 8-isoprostanes as biomarkers for oxidative stress with LC/MS/MS technique and to apply the method for human urine samples. Analyzed compounds for urinary oxidative stress markers were 7 stereo-isomers of prostaglandins and the internal standard (iso-$PGF_{2{\alpha}}-d_4$) was used to adjust the recovery rate. The method for determining urinary iso-$PGF_{2{\alpha}}$ consisted of solid phase extraction and LC/MS/MS detection. Separation of isomers of prostaglandins completed by porous graphitic carbon column and buffer solution. Detection limits for urinary markers of oxidative stress, iso-$PGF_{2{\alpha}}$ with LC/MS/MS were 0.01 ng/ml by S/N ratio 3 and 0.028 ng/ml by calculated as to FDA method. The recovery (92.8~101.9%) and precision (8.8~20.7%) of analysis were feasible for detecting iso-$PGF_{2{\alpha}}$ in real human urine samples. We detected 4 isomers of prostaglandins in human urine samples. Mean (standard deviation) of urinary iso-$PGF_{2{\alpha}}$ concentration were 0.231 (0.117) ng/mg creatinine for smoking group and 0.154 (0.082) ng/mg creatinine for non-smoking group.

• The Korean Journal of Pharmacology
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• v.12 no.2 s.20
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• pp.43-49
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• 1976

The facts that $PGE_2$ produced diuresis in the rabbit when given into a lateral ventricle of the brain and that $PGF_{2{\alpha}}$ is abundantly found in the brain prompted us to investigate the effects of $PGF_{2{\alpha}}$ introduced directly into the ventricle on the renal function. $PGF_{2{\alpha}}$ given intraventriculary in doses of $10{\mu}g\;and\;100{\mu}g$ elicited prompt diuresis, 10-fold increase of sodium excretion and two-fold increment of potassium excretion. Free water reabsorption also increased along with the increased osmolar clearance. Neither renal plasma flow nor glomerular filtration rate did change significantly. This, along with the fact that the percentage of reabsorbed sodium filtered decreased from 99.5 to 93.9, indicates the tubular site of the diuretic and natriuretic action. Atropine pretreatment did not influence the renal effects of intraventricular $PGF_{2{\alpha}}$. Intravenously administered $PGF_{2{\alpha}}$ in doses of 30 to $100{\mu}g$ did not produce any significant change in renal function. Intraventricular $PGF_{2{\alpha}}$ had no effect on the systemic blood pressure, whereas intravenous administration brought about a transient hypotension. These observations suggest that $PGF_{2{\alpha}}$ induces diuresis and natriuresis via central mechanism, that the site of the action resides in renal tubules, and that the reabsorption of sodium is inhibited in the proximal tubule, possibly through mediation of certain humoral agent. Overall, it is suggested that $PGF_{2{\alpha}}$ might play a roll in regulating renal function through the center.

• Journal of Veterinary Clinics
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• v.20 no.3
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• pp.384-388
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• 2003

To establish the method of estrus induction using the injection of PGF$_2$$\alpha$ㆍ the 60 dairy cows treated by PGF$_2$$\alpha$ㆍ at 6, 8, 10, 12, 14, and 16 day of estrous cycle, recpectively. The plasma progesterone concentrations at day of injection of PGF$_2$$\alpha$ㆍ were $1.5\pm$1.3 (mean$\pm$SD) ng/ml at 6 days of estrous cycle, 3.7$\pm$1.4 ng/ml at 8 days, 6.5$\pm$1.8 ng/ml at 10 days, 7.9$\pm$2.0 ng/ml at 12 days, 6.5$\pm$2.5 ng/ml at 14 days, and 2.5$\pm$1.2 ng/m] at 16 days, respectively. The percentages of induction of estrus after PGF$_2$$\alpha$ㆍ treatment were 52.8% at 3th day after treatment, 21.7% at 4th day, 8.3% within 2th day, and ] 7.2% after 5th day, respectively. The percentages of cows conceived at first service after induction of estrus were 73.3% on 16 days of estrous cycle at treatment, 66.7% on 10 and 14 days, 63.3% on 8 and 12 days, and 53.3% on 6 day, respectively. The percentages of cows conceived on first service were 70.5% at 3th day after induction, 66.7% at 4th day and 65% at 5th day, respectively. These results suggest that the PGF$_2$$\alpha$ㆍ treatment at regressing stage of corpus luteum showed high induction of estrus and pregnancy rate, and there were high induction of estrus and pregnancy rate at 3th and 4th day after PGF$_2$$\alpha$ㆍ treatment.

• Korean Journal of Animal Reproduction
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• v.5 no.2
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• pp.56-59
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• 1981

The sows which had not returned estrus since 15 to 152 days after weaning were treated with PMSG or PGF2$\alpha$ to induce estrus and inseminated to conceive on that estrus. 1. Eight (83%) among 12 sows treated with PMSG came into estrus on average 5.3 days after treatment. 2. The one sow which didn't show any estrus by treatment of PMSG showed an estrus by intramuscular use of 10mg PGF2$\alpha$ and the other one showed an estrus by another intramuscular use of 1000 I.U. of PMSG at 15 days after first injection of PMSG. 3. The one sow injected with Vit. A.D.E. complex(Injacom) (Vit. A 500,000 I.U., Vit. D. 75,000 I.U., and Vit. E. 50 I.U. per ml) on 6 days before PMSG injection showed an estrus on 6 days after PMSG injection and farrowed 8 piglets. 4. Five among 10 sows showed an estrus by treatment of PMSG or PGF2$\alpha$ were pregnant and litter size of the four farrowed sows averaged 9.5.

• Korean Journal of Animal Reproduction
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• v.9 no.1
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• pp.66-71
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• 1985

After the induction of estrus by the PGFF2$\alpha$-GTH combination on the 118 repeat breeder cow during of 6-13 days of the estrus cycle, the effects of GTH, antibiotics, antibiotics-GTH combination were compared. The results of this study, the following conclusion were obtained: 1. The rate of estrus was reduced the administration of GTH before the administration of PGF2$\alpha$. 2. The rate of ovulation delayed was 24.6% in the group with the administration GTH, but 34.1% without the administration GTH. Duration of ovulation was shortened by GTH. 3. The impregnation induction by the administration of PGF2$\alpha$, PGF2$\alpha$-antibiotics, PGF2$\alpha$-GTH-antibiotics were 41.7%, 69.5%, 55.6% and 80.8% respectively injection in the uterus was more effective than the administration. Before or after the A.I., the above combination was most effective. 4. In antibiotics sensitivity test in vitro of the intravagina bacteria, 5.0%, 12.7% and 60.0% of bacteria were sensitive to Penicilline, Streptomycin and Gentamicin respectively.

• Reproductive and Developmental Biology
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• v.31 no.4
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• pp.249-252
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• 2007

The objective of this study was to determine the effect of administration of Prostaglandin $F_2{\alpha}$ ($PGF_2{\alpha}$) on semen collection training and semen characteristics in sexually inexperienced boars. Boars were moved individually to a semen collection pen and were trained to mount dummy sow. During the first and second semen collection secessions, 4 out of 17 boars and 4 out of remaining 13 boars allowed collection of semen. The 9 boars that failed semen collection from first 2 attempts received immediately 15 mg of $PGF_2{\alpha}$ i.m. (intramuscular injection) upon entering the collection pen for semen collection resulted in successful semen collection from all 9 boars. Total numbers of spermatozoa were higher in $PGF_2{\alpha}$ treated boars but there was no significant difference in % motility kinematics characteristics between control and $PGF_2{\alpha}$ treated groups during 72 hr period. Overall, administration of $PGF_2{\alpha}$ in sexually inexperienced boars increased the sex drive and facilitated the mounting activity to the dummy sow for semen collection.

• Journal of Embryo Transfer
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• v.25 no.3
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• pp.133-139
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• 2010

The purpose of this study was to determine the relationship between conception rate and other parameters (body condition score; BCS, progesterone concentrations and follicle size) before estrus induction with $PGF_2{\alpha}$. The conception rate in cows with (2.75, 2.75 to 3.25 and 3.25), BCS regardless of AI (artificial insemination) time was 47.5, 67.5% and 48.5% at $PGF_2{\alpha}$ injection, respectively. The conception rate regardless of BCS was 59.0% in cows inseminated based on detected estrus, and 46.2% in cows inseminated at 72 to 80 hours (timed artificial insemination, TAI) after $PGF_2{\alpha}$ injection. The conception rate regardless of AI time was 43.0% in cows with low progesterone concentrations (less than 1.0 ng/ml), and 67.5% in cows with high progesterone concentrations (more than 1.0 ng/ml) at $PGF_2{\alpha}$ injection. The conception rate regardless of progesterone concentrations was 59.9% in cows inseminated based on detected estrus, and 48.1% in cows of TAI after $PGF_2{\alpha}$ injection. The conception rate regardless of AI time was 36.0% in cows with small dominant follicles (less than 5 mm), 56.0% in cows between 5 mm to 10 mm of follicle size, and 65.5% in cows with large dominant follicles (more than 10 mm) at $PGF_2{\alpha}$ injection, respectively. The conception rate regardless of follicle size was 57.3% in cows inseminated based on detected estrus, and 47.6% in cows of TAI after $PGF_2{\alpha}$ injection. These results indicated that if the cows with BCS 2.75 to 3.25, active corpus luteum, and/or large dominant follicle (more than 10 mm) are used for estrus induction, the conception rate will be greater.

• The Korean Journal of Pharmacology
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• v.27 no.2
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• pp.183-189
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• 1991

The present study was performed to elucidate the factors which modulate uterine prostaglandin synthesis during the implantation period in rats, by employing delayed implantation model. Administration of estradiol sharply increased uterine cAMP concentration 4 hrs later during the delayed implantation process. Concentrations of uterine PGE and $PGF_2{\alpha}$ were increased at 12 hrs after the estradiol treatment although an increase in $PGF_2{\alpha}$ was not statistically significant. The concomitant treatment of indomethacin with estradiol significantly suppressed estradiol-induced PGE and $PGF_2{\alpha}$ at 12 hrs, while uterine cAMP concentration was not suppressed. The treatment of dbcAMP without estradiol gradually increased uterine PGE and $PGF_2{\alpha}$ showing the maximum 8 hrs later, suggesting that cAMP minics estradiol effect on uterine prostaglandin synthesis during the implantation process. Furthermore, the pretreatment of theophylline, phosphodiesterase inhibitor, induced significantly greater concentrations of uterine PGE and $PGF_2{\alpha}$, compared with estradiol-only treated group. These results suggest that estradiol stimulates uterine prostaglandin synthesis and this process may be mediated by an elevation of cAMP during the delayed implantation process in rats.

• Journal of Embryo Transfer
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• v.27 no.3
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• pp.133-139
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• 2012

This study was carried out to develop the useful inducing method of estrus for Korean native cows. Under the condition of estrus induction by administering $PGF_{2{\alpha}}$ for the cows in which corpus luteum (CL) in ovaries was detected by ultrasonography, ovarian responses and the changes of progesterone ($P_4$) concentration against $PGF_{2{\alpha}}$ compared with conception rate were observed in cows and heifers. In inducing estrus administering $PGF_{2{\alpha}}$. to the cows which has corpus luteum in ovaries, ovarian reponses, the changes of progesterone concentration, and conception rate were identified and compared. The results attained from the studies were as follows. Significant decreases of CL in size over time after $PGF_{2{\alpha}}$ administration were detected in both cow and heifer groups (p<0.001), but not different between groups in the CL regression rate (p>0.05). In addition, the percentage changes relative to the plasma $P_4$ concentration on day 1 after $PGF_{2{\alpha}}$ treatment were decreased to below 1ng/ml. The growth rate of follicle was observed as 31% on day 1 and 42% on day 2 in cows, and 34% on day 1 and 97% on day 2 in heifers, resulting that growth of heifers are faster than that of cows (p<0.05). The conception rate after $PGF_{2{\alpha}}$ treatment were 60.5% and 64.2% in cows and heifers, respectively. It also indicated that the conception rate after estrus observation with $PGF_{2{\alpha}}$ injection was as high as 66.6% while that with timed-artificial insemination (TAI) regardless of the estrus observation was 56%, which means the pregnancy rate of artificial insemination after estrus observation was higher than that of TAI (p<0.05). In the result of all above, there were significant decreases in CL size and the plasma $P_4$ concentration by days but rapid growth in follicles, which has no differences in cows and heifers. The conception rate was commonly high after estrus observation and more than 50% under TAI.