• 한국통신학회논문지
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• 제34권10B호
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• pp.1076-1085
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• 2009

본 논문에서는 3차원 동영상의 시간적 중복성과 룩업 테이블을 이용한 효과적인 3차원 동영상 홀로그램 생성방법을 제안한다. 기존의 N-LT를 이용한 홀로그램 생성 기법은 미리 생성된 요소 프린지 패턴을 단순 이동 시켜 물체에 대한 홀로그램을 고속으로 생성할 수 있게 하였다. 하지만, 3차원 동영상 홀로그램의 경우는 정지영상에 대한 홀로그램에 비해 방대한 양으로 인하여 실시간 홀로그램을 생성하는데 많은 시간이 필요하게 되어 실직적인 제한이 있었다. 반면 이러한 3차원 동영상은 인접한 프레임은 유사한 정보를 갖는다는 시간적 중복성을 갖고 있고, 이러한 특성을 동영상 압축에 이용하고 있다. 따라서 본 논문에서는 이러한 3차원 동영상의 시간적 중복성을 이용하여 계산하여야 하는 데이터의 양을 현저하게 줄임으로써 3차원 동영상에 대한 홀로그램을 고속으로 생성할 수 있는 새로운 방법을 제안하고 실험을 통하여 검증하였다.

• Bulletin of the Korean Chemical Society
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• 제34권11호
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• pp.3284-3288
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• 2013

This paper describes first development and validation of pentafluorophenylprophyl ligand-based liquid chromatography coupled to tandem mass spectrometry (PFPLC-MS/MS) method to determine metformin, a highly polar compound, in human plasma. Metformin and Phenformin (internal standard) were extracted from human plasma 50 ${\mu}L$ with a single-step protein precipitation. The chromatographic separation was performed using a linear gradient elution of mobile phase involving 5.0 mM ammonium formate solution with 0.1% formic acid (A) and acetonitrile (B) over 3.0 min of run time on a Phenomenex Luna PFP column. The detection was performed using a triple-quadrupole tandem mass spectrometer (Waters Quattro micro) with electrospray ionization in the mode of positive ionization and multiple-reaction monitoring (MRM). The developed method was validated with 5.0 ng/mL of lower limit of quantification (LLOQ). The calibration curve was linear over 5-3000 ng/mL of the concentration range ($R^2$ > 0.99). The specificity, selectivity, carry-over effect, precision, accuracy and stability of the method met the acceptance criteria. The method developed in this study had had rapidness, simplicity and ruggedness. The reliable method was successfully applied to high throughput analysis of real samples for a practical purpose of a pharmacokinetic study.

• 대한한의학회지
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• 제36권2호
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• pp.8-20
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• 2015

Objectives: This study aims to evaluate the efficacy of pharmacopuncture in the treatment of acute peripheral facial paralysis (APFP) through a meta-analysis of randomized controlled trials to assess the clinical evidence for pharmacopuncture as a treatment for PFP. Methods: Literature was searched using databases. The search terms used were "pharmacopuncture," "sweet BV," "apipuncture," "apitoxin," "injection acupuncture," and "herbal acupuncture" in English and "bongchim," "bongyakchim," and "yakchim" in Korean. The RevMan 5.2 program was used for meta-analysis. Results: In all, 1902 studies were screened and the full texts of 219 articles were reviewed. Of these, nine studies were included in the systematic review and nine studies were included in the meta-analysis. Treatments utilizing bee venom, sweet bee venom (SBV), Soyeom, Calculus Bovis Fel Ursi Moschus (BUM), Hominis Placenta, and Suseunghwagang (SSHG) were observed in nine RCTs. In the forest plot, the diamond favors the PA treatment against the control treatment. (95% CI: 0.34 [0.08, 0.60], Z = 2.57, P = 0.01), which Our meta-analysis in RevMan 5.2 confirmed the significant efficacy of PA in treating APFP. Conclusion: Our systematic review and meta-analysis suggest that treatments using Soyeom, bee venom, and BUM are effective in APFP and they reduce the duration of postauricular pain occurring at the acute stage of APFP. However, due to small effect size, further investigation is needed.

• Journal of Biomedical and Translational Research
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• 제19권4호
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• pp.130-139
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• 2018

This study aimed to analyze a high-performance liquid chromatography (HPLC) separation using a pentafluorophenyl column of parent drug hydroxychloroquine (HCQ) and its active metabolite, desethylhydroxchloroquine (DHCQ) applying to determine bioequivalence of two different formulations administered to patients. A rapid, simple, sensitive and specific liquid chromatography-tandem mass spectrometry (LC-MS/MS) method has been developed and validated for bioanalysis of HCQ and its metabolite DHCQ in human whole blood using deuterium derivative $hydroxychloroquine-D_4$ as an internal standard (IS). A triple-quadrupole mass spectrometer was operated using electrospray ionization in multiple reaction monitoring (MRM) mode. Sample preparation involves a two-step precipitation of protein techniques. The removed protein blood samples were chromatographed on a pentafluorophenyl (PFP) column ($50mm{\times}4.6mm$, $2.6{\mu}m$) with a mobile phase (ammonium formate solution containing dilute formic acid) in an isocratic mode at a flow rate of 0.45 mL/min. The standard curves were found to be linear in the range of 2 - 500 ng/mL for HCQ; 2 - 2,000 ng/mL for DHCQ in spite of lacking a highly sensitive MS spectrometry system. Results of intra- and inter-day precision and accuracy were within acceptable limits. A run time of 2.2 min for HCQ and 2.03 min for DHCQ in blood sample facilitated the analysis of more than 300 human whole blood samples per day. Taken together, we concluded that the assay developed herein represents a highly qualified technology for the quantification of HCQ in human whole blood for a parallel design bioequivalence study in a healthy male.

• Genomics & Informatics
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• 제21권3호
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• pp.35.1-35.12
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• 2023

The Bacillus cereus group, also known as B. cereus sensu lato (B. cereus s.l.), is composed of various Bacillus species, some of which can cause diarrheal or emetic food poisoning. Several emerging highly heat-resistant Bacillus species have been identified, these include B. thermoamylovorans, B. sporothermodurans, and B. cytotoxicus NVH 391-98. Herein, we performed whole genome analysis of two thermotolerant Bacillus sp. isolates, Bacillus sp. B48 and Bacillus sp. B140, from an omelet with acacia leaves and fried rice, respectively. Phylogenomic analysis suggested that Bacillus sp. B48 and Bacillus sp. B140 are closely related to B. cereus and B. thuringiensis, respectively. Whole genome alignment of Bacillus sp. B48, Bacillus sp. B140, mesophilic strain B. cereus ATCC14579, and thermophilic strain B. cytotoxicus NVH 391-98 using the Mauve program revealed the presence of numerous homologous regions including genes responsible for heat shock in the dnaK gene cluster. However, the presence of a DUF4253 domain-containing protein was observed only in the genome of B. cereus ATCC14579 while the intracellular protease PfpI family was present only in the chromosome of B. cytotoxicus NVH 391-98. In addition, prophage Clp protease-like proteins were found in the genomes of both Bacillus sp. B48 and Bacillus sp. B140 but not in the genome of B. cereus ATCC14579. The genomic profiles of Bacillus sp. isolates were identified by using whole genome analysis especially those relating to heat-responsive gene clusters. The findings presented in this study lay the foundations for subsequent studies to reveal further insights into the molecular mechanisms of Bacillus species in terms of heat resistance mechanisms.

• Imaging Science in Dentistry
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• 제30권3호
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• pp.155-158
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• 2000

1. 일반방사선사진과 칼라화한 방사선사진의 비교에서 각각 필름에서 진단을 시행할 때 ROC해석법에서는 true positive fraction (TPF), false positive fraction (FPF)를 매개변수로 하고 있으므로 우선 두가지 필름형태에 대해 각각 따로 다음과 같이 평가한다. 2. 판정기준 병변없다 A, 거의 없다 B, 모르겠다 C, 거의 있다 D, 있다 E 먼저 일반방사선사진에서 실제로 병소가 총있는 것이 50, 총없는 것이 50인데 위 판정기준 각각에 대해(equation omitted) 3. 곡선만들기 a.횡축은 FPF 종축은 TPF로 한 그래프를 plot를 한다. sensitivity 17/50 specificity 26/50 accuracy 43/100 b. 곡선만들기 프로그램을 이용하여 곡선을 만들시에는 TPF를 a에 입력하고 PFP를 b에 입력한다. 이 plot을 그릴 수 있는 프로그램은 http://www.members.tripod.co.kr/jdakim 또는 http://www.chosun.ac.kr/∼jdakim의 홈페이지내 공개자료실에서 다운 받으실 수 있습니다. (equation omitted) 이 프로그램에서 입력할 a, b의 값은 (equation omitted) 위와같이 입력하여 얻어진 일반방사선사진에서의 판독 결과 얻어진 곡선이 그래프에서 곡선이 된다. 이와 같은 커브를 컬러화한 사진 판독에서 똑같이 시행하여 ROC곡선(윗곡선)을 만든 다음 두 곡선을 비교하여 아래면적이 더 큰 쪽이 병소 판독에 우수하다고 결론짓는다.