• Horticultural Science & Technology
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• v.32 no.6
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• pp.879-886
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• 2014

This study was conducted to test in vitro the antagonistic effect of composted liquid manure (CLM) against soil-borne turfgrass pathogenic fungi, Rhizoctonia solani AG-2-2 (IIIB) (brown patch), R. solani AG-2-2 (IV) (large patch), and Sclerotinia homoeocarpa (dollar spot) for environmentally friendly turfgrass management. CLMs were collected from 9 livestock excretion treatment facilities around the country including Gunwi (GW), Hapcheon (HC), Hoengseong (HS), Icheon (IC), Iksan (IS), Muan (MA), Nonsan (NS), and Yeoju (YJ). CLMs of IC, GW, and IS showed s ignificant (p < 0.05) mycelium growth inhibition that was 17.8%, 20.4%, and 48.0% against R. solani AG-2-2 (IIIB), R. solani AG-2-2 (IV), and S. homoeocarpa, respectively. A t otal of 110 bacterial isolates were obtained from the CLMs that showed antagonistic effects. Among them, 5, 4, and 10 microbe isolates showed promising antifungal activity against mycelium growth of R. solani AG-2-2 (IIIB), R. solani AG-2-2 (IV), and S. homoeocarpa, respectively. The bacterial isolates ICIIIB60, GWIV70, and ISSH20 effectively inhibited the mycelial growth of three soil-borne turfgrass pathogens. Selected bacterial isolates were identified as Alcaligenes sp., Bacillus licheniformis Ab2, and B. subtilis C7-3 through 16s rDNA gene sequence analysis. Among 5 fungicides, the most compatible fungicide with ICIIIB60, GWIV70, and ISSH20 was tebuconazol, toclofos-methyl and toclofos-methyl, respectively. These findings suggested that CLMs could be effectively used not only as organic liquid fertilizer sources but also as biological control agents for soil-borne turfgrass diseases such as brown patch, large patch, and dollar spot.

• Journal of Mushroom
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• v.11 no.4
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• pp.201-207
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• 2013

Fungus gnats are usually found in mushroom farm and have recently become important pest because they can cause severe damage and reduce the production on shiitake mushroom. Usually shiitake mushrooms are cultivated on both oak bed logs and in the artificial sawdust beds in greenhouses. Using yellow sticky trap, the dipteran species in shiitake mushroom farm were collected from May to September in Kyonggi-do and Chungcheong-do in 2013. To identify the main species of fungus gnat on the shiitake farm in Korea, the collected samples were determined the sequence of cytochrome c oxidase subunit I (COI) by DNA barcoding. The phylogeny based on maximum likelihood analyses from COI sequence showed that Bradysia difformis and B. alpicola were main species of shiitake bed log and Scatopsidae sp. and B. difformis were dominant species of sawdust beds.

• Korean journal of applied entomology
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• v.51 no.4
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• pp.469-477
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• 2012

Ostrinia larvae feed the pods and stem of red bean and seriously damage the bean production from farmers. In this study we investigated biological and developmental characteristics including field collection, host feeding preference, artificial rearing diet, morphological and molecular taxonomical identification, and pheromone analysis for an Ostrinia sp. in Korea. The male adults have massive tibia in the middle legs and 3-lobed uncus in the genitalia. The partial nucleotide sequences of mitochondrial cytochrome oxidase I (COI) and II (COII) were not corresponded to those DNA sequences from other Ostrinia species reported previously in Japan and China. Host plants for this species are also different from the previous species reported. In the gas chromatography (GC) analyses, (Z)-9-tetradecenyl acetate was not detected from the pheromone gland of our species while the component as a sex pheromone was found in O. zaguliaevi and O. zealis, With taken results, we conclude this Ostrinia species in Korea is Ostrinia scapulalis or closely related species. When larvae collected in a fall were incubated in the outdoor condition, they emerged to adult between June and July in the next year. The result indicates that the winter diapause could be started in late larval stage. In addition, we developed a semi-synthetic artificial diet adopted for mass rearing of the O. scapulalis in laboratory.

• Proceedings of the Korea Water Resources Association Conference
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• 2012.05a
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• pp.782-782
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• 2012

지금까지 우리나라에서는 도시하수, 공장폐수 등의 점오염원에 국한하여 중점적으로 수질관리를 실행하여 부분적으로 효과를 얻을 수 있었으나, 하천과 호소의 수질은 크게 향상되지 않고 있다. 이는 급속한 도시화와 산업발달로 토지개발이 가속화되고 대지, 도로, 주차장 등 불투수층 면적이 늘어남에 따라 비점오염원에 의한 하천, 호소의 수질영향도가 커지고 있기 때문이다. 인구증가로 인해 물 사용량 뿐만 아니라 이에 따라 배출되는 오염원의 종류 및 오염부하량 역시 함께 증가하고 있다. 장래의 수질관리 성공여부는 비점오염원의 효율적인 관리여부가 큰 변수로 작용할 것으로 본다. 따라서 공공수역의 수질관리를 위해서는 토지이용과 지역특성을 고려한 비점오염원 부하량의 합리적인 조사, 오염 부하량 절감을 위한 관리기술의 개발, 비점오염원 관리정책의 개발 및 수질모형을 이용한 정확한 수질예측 등이 필요하다. 따라서 본 연구에서는 공간정보를 바탕으로 한 낙동강 유역에서의 비점오염원 정량화 분석을 수행하고자 한다. 우선 대상유역으로 낙본 G유역을 선정하여 이에 대한 조사를 통해 점오염원의 실측자료를 구축하고 이를 HSPF의 입력하여 모의를 수행하여 대상유역에 대한 실측치를 이용해 모형의 보정과 검증을 수행한다. 이러한 과정을 통해 도출된 결과는 대상유역의 총 오염량을 의미한다. 따라서 위의 과정에서 도출된 매개변수를 이용하고, 점오염원을 제거한 뒤 모의를 재수행하여 나온 결과가 대상유역의 비점오염원의 양이라 판단하였다. 모의 결과 대상유역인 낙본 G유역에서 약 39% 정도의 비점오염원 비율을 보였다. 그러나 수질 및 유량 관측치를 지금까지는 국립환경과학원 낙동강물환경연구소 유량측정데이타를 사용하고 있는데 이 자료는 8일 이상 간헐적으로 측정이 수행되고 있다. 따라서 검 보정 대상이 되는 실측치의 자료의 부족과 부정확한 유역이 있음이 한계점으로 작용한다. 그러므로 추후의 신경망 모형이나 기타 실측치 보간에 있어서의 신뢰도를 높이는 기법 개발이나 측정제도의 보편적인 기술의 증대도 앞으로의 모델링에 있어서 중요할 것으로 판단된다. 또한 유역수질모형의 모델링 과정에서 좀 더 신뢰도 높은 측정자료와 그 측정자료를 활용하여 PEST 보정기법을 적용한다면 더욱 정확한 예측이 이루어질 수 있을 것이며, 본 연구에서의 평가방법을 바탕으로 유역수질모델링이 이루어진다면 보다 더 정확성 높은 비점오염원 정량화와 수질 예측이 수행될 수 있을 것이며 더 나아가 오염총량제의 수행에 효과적으로 적용될 것으로 판단된다.

• Journal of Life Science
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• v.15 no.6 s.73
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• pp.916-922
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• 2005

Parkin, known as an E3 ubiquitin ligase, has essential role in protein quality control, and its severe dysfunction leads to neurodegenerative disorders. Human Parkin was excessively degraded when expressed in Escherichia coli under the conventional induction condition ($37^{\circ}C$ culture condition with 0.5 mM IPTG). To optimize the induction and culture conditions for recombinant human Parkin and develop a rapid method for the Parkin purification, we expressed Parkin by using PCEX system at the different culture temperatures and IPTC concentrations. The intact Parkin protein was purified to approximately $90\%$ purity with suitable amounts of protein under the optimal culture condition ($25^{\circ}C$E with 0.01 mM IPTG). Additionally, we constructed various parkin plasmids with different tagging systems and investigated their expression patterns in HEK293 cells. We found that the proteolytically sensitive site is localized within a ubiquitin-like domain of Parkin. This study developes a method for generating useful reagents to investigate biochemical properties of Parkin.

• Journal of Bio-Environment Control
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• v.13 no.1
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• pp.16-20
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• 2004

Aphis gossypii Glover(Homoptera: Aphididae) is an serious pest on various crops. This experiment was conducted to evaluate the effect of A. gossypii Glover on the growth of oriental melon and the plant recovery after removing A. gossypii. Visible damage symptom caused by A. gossypii feeding to oriental melon was leaf distortion and stunting. A. gossypii feeding for 20 days on oriental melon significantly reduced the growth of oriental melon with increasing A. gossypii density level. Plant growth was decreased by 63${\sim}$69%, 62${\sim}$88% and 49${\sim}$70% in plant height, leaf area and dry weight, respectively. During 10 day after aphids removal, the infested leaves remained stunt, however, new shoot and leaf recovered gradually. By 20 day, plant height, leaf area and dry weight substantially increased in 5 aphid per plant. Plant recovered rapidly with day and among aphid density level, 5 aphid per plant showed rapid recovery. When plant were infested with several density of aphid per plant, sugar contents of total leaves were not significantly different between aphid density level. Whereas, sugar contents of lear infested with aphid per leaf were decreased with increasing cumulative aphid-days.

• Korean Journal of Breeding Science
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• v.43 no.1
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• pp.56-61
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• 2011

Brown planthopper(BPH) is one of the most destructive insect pests of rice. The use of genetically resistant cultivars has proven to be a more economical, efficient and environment friendly means to combat this pest. This study was carried out to investigate the relationship between BPH resistant gene, Bph18 and yield components of rice using DH(doubled haploid) lines derived from 'Saenuri'/SR30071-3-7-23-6-1-1-1. SR30071-3-7-23-6-1-1-1 line has Bph18 gene derived from wild species, Oryza australiensis. BPH resistant gene, Bph18 shortened heading days, enlarged culm length and panicle length and reduced ratio of ripened grains of rice. The results indicate that backcrossing breeding is necessary to develop elite cultivars carrying Bph18 gene.

• Journal of Sericultural and Entomological Science
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• v.28 no.1
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• pp.43-53
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• 1986

Among many microbial pesticides, Bacillus thuringiensis is one of the most hopeful pesticide and some commercial products have been appearing on the market. Because these commercial products contain living spores and toxins of the organism, there is a danger that living spores of B. thuringiensis may be scattered by wind and cause a great damage in the sericulture areas. In order to avoide these risks it is desirable to select the strain which has low pathogenicity to the silkworm, and at the sometime being highly pathogenic to the pest insects. Thus this study has been carried out to acquire some basic informations about the procedure of desicable strain selection. Three strains of B. thuringiensis var. kurstaki, var. dendrolimus and var. aizawai were used for the pathogenicity test on the silkworm, Bombux mori and the fall webwarm, Hyphantria cunea. Those strains were investigated by the agarose gel electrophoresis patterns of plasmid DNA determine whether mutation had occured. Pathogenicity tests were carried out of using isolated crystal proteins and spore-crystal protein to mixtures of each strain, seperatively. In case of using spore-crystal protein mixture, the order of pathogenicity in varities of B. thuringiensis against B.mopri and H.cunea were kurstaki, aizawai, dendrolimus and kurstaki, dendrolimus, aizawai, respectively. But using isolated crystal proteins, dendrolimus had the highest toxicity to H. cunea and the lowest toxicity to B. mori among tested three strains. From the above results, dendrolimus was presumed the most desirable straing for using microbial pesticide.

• Journal of the Korean Society of Environmental Restoration Technology
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• v.24 no.6
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• pp.1-13
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• 2021

This study was performed to investigate the ecosystem service patterns in relation to climate change acceleration utilizing big data analysis. This study aimed to use big data analysis as one of the network of views to identify convergent thinking in two fields: climate change and ecosystem service. The keywords were analysed to ascertain if there were any differences in the perceiving problems, policy direction, climate change implications, and regional differences. In addition, we examined the research keywords of each continent, the centre of ecosystem service research, and the topics to be referred to in domestic research. The results of the analysis are as follows: First, the keyword centrality of climate change is similar to the detailed indicators of The Intergovernmental Science-Policy Platform on Biodiversity and Ecosystem Services (IPBES) regulations, content, and non-material ecosystem services. Second, the cross-analysis of terms in two journals showed a difference in value-oriented point; the Ecosystem Service Journal identified green infrastructure as having economic value, whereas the Climate Change Journal perceives water, forest, carbon, and biodiversity as management topics. The Climate Change Journal, but not the former, focuses on future predictions. Third, the analysis of the research topics according to continents showed that water and soil are closely related to the economy, and thus, play an important role in policy formulation. This disparity is due to differences in each continent's environmental characteristics, as well as economic and policy issues. This fact can be used to refer to the direction of research on ecosystem services in Korea. Consistent with the recent trend of expanding research regarding the impacts of climate change, it is necessary to study strategies to scientifically predict and respond to the negative effects of climate change.

• Korean journal of applied entomology
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• v.61 no.2
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• pp.349-356
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• 2022

Fall armyworm, Spodoptera frugiperda, is a invasive migratory pest of native to the tropical and sub-tropical regions that has recently invaded Korea with damage to cornfield. The study was conducted to investigate the development periods of S. frugiperda on artificial diet at six different temperatures. The developmental period from eggs to adult at 18, 21, 24, 27, 30 and 32℃ was 79.8, 54.2, 34.3, 28.4, 24.6 and 24.0 days, respectivery and decreased with increasing temperature. The pupal periods of females were shorter than males. Most of the larvae developed through six instar, but the ratio of 7 or more instar increased at low temperatures. The relationship between the development rate and temperature was fitted with by linear regression analysis. The lower development threshold for egg and larva development was 12.9℃ and 11.3℃, respectively, 12.6℃ for pupae and 11.8℃ for egg-to-adult development. The effective accumulative temperature for the development of the respective life cycle stages were 37.0 DD for eggs, 286.3 DD for larvae, 132.2 DD for pupae and 456.8 DD egg-to-adult development.