• Journal of the Korean Society of Food Science and Nutrition
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• v.39 no.10
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• pp.1522-1527
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• 2010

This study was conducted to find out the minimal time needed for detection of Salmonella spp. which exist at very low concentration in foods by using real-time PCR. The sal-F and sal-R sequences were used as primers and sal-P was used as a probe. The detection limit of Salmonella spp. was $3.77{\times}10^2\;cfu/mL$ in buffered peptone water (BPW). Microbial growth was monitored after artificially inoculated Salmonella spp. into BPW. The obtained growth curve was well fitted with the equation, y＝$0.0127x^2$+0.5927x-0.4317 ($R^2$=0.99), if assuming that 1 cell exists in 25 g sample (0.04 cfu/mL). The microbial concentration will be reduced to 10 fold by adding BPW during sample treatment, so actual initial concentration at the starting point of enrichment is 0.004 cfu/mL. At this condition, real-time PCR detection would be possible only when microbial concentration increase occurs to exceed the detection limit (377 cfu/mL). The time needed for microbial increase was calculated from the growth curve equation as 7 hours and 20 minutes. Therefore the total time required for detection was less than 10 hours including the PCR operating time.

• Journal of Plant Biotechnology
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• v.35 no.1
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• pp.55-61
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• 2008

Previous studies on genetic transformation of chrysanthemum using cold regulated gene (BN115) have been conducted and the PCR and Real-Time PCR based method to determine the presence of the transferred cold regulated gene in the chrysanthemum was established. To check whether over-expression of BN115 gene in transgenic chrysanthemum will enhance their tolerance to cold stress, the transgenic chrysanthemum were grown under low temperature condition and several cold signalling including growth characteristics, stoma size and shape, SPAD value and ion leakage test were investigated. The transgenic chrysanthemum in the low temperature growth chamber grow much faster in term of the height, number and size of the leaves than those of wild-type plants and damage of transgenic plant caused by the low temperature was much less than that of wild-type plants. The stoma type and size of transgenic plant leaves grown at $5^{\circ}C$ were much similar to of wild-type plant cultured on $25^{\circ}C$ It has been found that SPAD value of transgenic plants was much higher than those of wild-type, but the EC density being lower under low temperature condition.

• Journal of Veterinary Clinics
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• v.36 no.4
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• pp.200-206
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• 2019

This study was performed to assess the anti-inflammatory and cartilage regenerative effects of platelet-rich plasma (PRP) on canine chondrocytes. Proliferation and migration assays under both normal and lipopolysaccharide (LPS)-induced inflammatory conditions were performed with various concentrations of PRP (1% to 10%). The expression levels of genes related to osteoarthritis were evaluated in the following groups: PRP group, LPS group and LPS + PRP group. mRNA expression levels were detected using real-time polymerase chain reaction (RT-PCR). Proliferation assays showed significantly enhanced proliferation in all PRP-treated groups compared with the no serum group. Compared with 10% fetal bovine serum (FBS), PRP concentrations above 3% in the normal condition and 1% to 7% PRP in the LPS-induced inflammatory condition were found to significantly promote chondrocyte proliferation. In the normal condition, all PRP-treated groups showed significantly increased cell migration compared with the no serum group. Chondrocyte migration was decreased with LPS-induced inflammation, but PRP treatment resulted in significantly enhanced migration compared with the other groups in this condition. According to RT-PCR, the LPS + PRP group showed significantly higher levels of COL1A1, IL-6, aggrecan and lower levels of $TNF-{\alpha}$, MMP-1, MMP-3 mRNA expression compared to the LPS group. The results of this study suggest that PRP application can enhance the proliferation and migration of canine chondrocytes and improve canine articular cartilage regeneration.

• Proceedings of the KSME Conference
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• 2007.05b
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• pp.2038-2043
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• 2007

Analysis of the internal state of the blast furnace is needed to predict and control the operating condition. Especially, it is important to develop modeling of blast furnace for predicting cohesive zone because shape of cohesive zone influences on overall operating condition of blast furnace such as gas flow, temperature distribution and chemical reactions. Because many previous blast furnace models assumed cohesive zone to be fixed, they can't evaluate change of cohesive zone shape by operation condition such as PCR, blast condition and production rate. In this study, an axi-symmetric 2-dimensional steady state model is proposed to simulate blast furnace process using the general purpose-simulation code. And Porous media is assumed for the gas flow and the potential flow for the solid flow. Velocity, pressure and temperature distribution for gas and solid are displayed as the simulation results. The cohesive zones are figured in 3 different operating conditions.

• 한국연소학회:학술대회논문집
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• 2006.04a
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• pp.39-45
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• 2006

Analysis of the internal state of the blast furnace is needed to predict and control the operating condition. Especially, it is important to develop modeling of blast furnace for predicting cohesive zone because shape of cohesive zone influences overall operating condition of blast furnace such as gas flow, chemical reactions and temperature. because many previous blast furnace models assumed cohesive zone to be fixed, they can't evaluate change of cohesive zone shape by operation condition such as PCR, blast condition, and production rate. In this study, an axi-symmetric 2-dimensional steady state model is proposed to simulate blast furnace process. In this model, cohesive zone is changed by solid temperature range, FVM is used for numerical simulation. To find location of cohesive zone whole calculation procedure is iterated Until cohesive zone is converged. Through this approach, shape of cohesive zone, velocity, composition and temperature within the furnace are predicted by model.

• Journal of Food Hygiene and Safety
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• v.18 no.2
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• pp.79-86
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• 2003

Most of food poisoning is frequently raised from mass catering. Especially, staphylococci takes the large part of pathogenic agents which are related to the hygienic condition. Among total 98 samples, four staphylococci were isolated from food service environment such as drinking water (A), hands (D), refrigerator and apron (E) of 5 elementary school (A, B, C, D, E) in Gyeongnam Province. These isolated strains are characterized as 1 MRCNS (Methicilline Resistant Coagulase Negative Staphylococcus aureus) and 3 MSCPS (Methicilline Sensitive Coagulase positive Staphylococcus aureus). Also, production of enterotoxin B (sob gene) were examined by PCR which has known as a big problem because of their temperature resistance. Hence, PCR was performed on isolated 4 staphylococci. The all 4 isolated Staphylococcus aureus have 477 bp of seb gene. Antibiotics susceptibility test was completed on PCR detected strains. All strains were fully resistance to ampicillin and penicillin. The drinking water of A place has resistance to oxacilline, therefore this strain turned out to be MRSA (Methicilline Resistant Staphylococcus Aureus).

• Journal of Sericultural and Entomological Science
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• v.38 no.1
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• pp.7-12
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• 1996

Reproducible the random amplified polymorphic DNAs(RAPDs) patterns were obtained in the two silkworm strains(J111, Galwon) by adjusting concentration optimized of Taq DNA polymerase(one unit), dNTP(200$\mu$M), MgCl2(1.5mM) and template DNA(30ng). In addition, anealing temperature ranging 35$^{\circ}C$ to 42$^{\circ}C$ by the adjusted condition was investigated and fixed at 35$^{\circ}C$ in this study. Variation among individuals and between male and female of Jam 113 strain was not authorized. DNA polymorhpisms among silkworms were authorized by five RAPD markers using OPM04 random primer. Using the primer showing polymorhpims between parents(J111, Galwon) in thirty three individuals, RAPD-PCR for F2 analysis was performed and segregated 3 : 1 in the F2 population. Consequently, RAPDs detected in the parents were obtained as genetic markers, which can be used for construction of genetic map for this industrially particular insect, silkworm Bombyx mori

• Journal of Life Science
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• v.8 no.1
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• pp.8-13
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• 1998

The maize mitochondrial mutant (NCS2) is derived from homologous recombination between genes encoding NADH dehydrogenase subunit 4 and subunit 6. Plants from mitochondria mutants exhibited severe related growth and development including dwarfism and striping on the leaves. Aborted embryos from NCS2 mutants have been rescued and cultured on the N6 medium supplemented with 2,4-D 1 mg/l. Most calli from NCS2 aborted embryos showed slow growing pattern at first stage. However, upon continuous culturing them on the medium, those were segregated into mutant and normal callus lines. These segregations could be detected by using PCR method with three primers. Such segregation seems to be resulted from the preferential growth of normal cells over the mutant cells on the normal culture condition. Therefore, this method can be used for determining rate of indirect cytoplasmic segregation by estimating amplified band intensities. When NCS2 mutant callus lines cultured on regeneration medium, no adventitious shoot induction was observed. However, callus lines with more mitochondria induced adventitious shoots. These studies suggest that mitochondria NADH-dehydrogenase for electron transport in the inner membrane of mitochondria is essential for the differentiation and development of plants.

• Korean Journal of Veterinary Service
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• v.39 no.1
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• pp.29-34
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• 2016

This study was carried out to investigate the tick-borne pathogens from one hundred nineteen cattle farms (38 farms of Andong, 41 of Yeongju, 12 of Uiseong, 5 of Cheongsong, 5 of Yoengyang, 18 of Bonghwa) in northern areas of Gyeongbuk province by polymerase chain reaction (PCR). Among 119 cattle farms, the positive ratios against Babesia, Theileria, Anaplasma, Ehrlichia and Rickettsia were 3.4% (4/119), 10.1% (12/119), 6.7% (8/119), 1.7% (2/119) and 16.8% (20/119), respectively. Also, the PCR results revealed that 8 farms were positive for T. sergenti in positive of Theileria and 2 farms were positive for A. phagocytophilum in positive of Anaplasma. Therefore, further studies regarding vectors, environmental condition, interaction between domestic and wild animals and development of control program are needed to reduce the numbers of bovine tick-borne disease in northern areas of Gyeongbuk province.

• Korean Journal of Plant Tissue Culture
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• v.25 no.5
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• pp.323-327
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• 1998

The flavonoid biosynthetic pathway has been studied as a genetic model system, particularly in Petunia hybrida. In order to study the flavonoid biosynthetic pathway, we constructed a fusion gene system between Cauliflower Mosaic Virus (CaMV) 35S promoter and eggplant flavonoid 3', 5'-hydroxylase in pBI 121 plasmid. An optimal condition for plant regeneration was observed when internode explants were cultured on MS medium supplemented with IAA 0.2 mg/L plus BA 3 mg/L. For plant transformation internode explants of Petunia hybrida were precultured on BM medium supplemented with IAA 0.2 mg/L plus BA 3 mg/L. Putative transgenic plants were selected on medium containing kanamycin 50 mg/L plus cefotaxim 300 mg/L. Putative selected transformants were confirmed by amplification of selectable marker gene (nptII) by polymerase chain reaction (PCR) and Southern hybridization of flavonoid 3',5'-hydroxylase gene.