• Title/Summary/Keyword: PCR(polymerase chain reacion)

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A study on development of plastic vial tube for the DNA detection process (DNA 검출 공정 전용 플라스틱 튜브형 시험관 개발에 관한 연구)

  • Choi, Kyu-wan;La, Moon-woo;Gang, Jung-hee;Chang, Sung-ho
    • Design & Manufacturing
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    • v.11 no.3
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    • pp.35-40
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    • 2017
  • PCR(Polymerase chain reaction) is a technique to replicate and amplify a desired part of DNA. It is used in various aspects such as DNA fingerprint analysis and rare DNA amplification of an extinct animal. Especially in the medical diagnosis field, it provides various measurement methods at the molecular level such as genetic diagnosis, and is a basic tool for molecular diagnostics. The internal shape of the plastic vial tube for PCR analysis used in the DNA detection process, and the surface roughness and internal cleanliness can affect detection and discrimination results. The plastic vial tube demanded by the developer of the PCR analysis equipment should be changed to a structure that eliminates the residual washing solution in the washing process to ensure the internal cleanliness. Thus the internal structure and the internal surface design for improving the PCR amplification efficiency are key issues to develop the plastic vial tube for the DNA detection process.

Plant Regeneration and Expression of Mouse Adenosine Deaminase Gene in Transgenic Hot Pepper (Capsicum annuum L.) Plants (형질전환된 고추( Capsicum annum L.) 식물체의 Mouse Adenosine Deaminas 유전자 발현)

  • 양덕춘;이계연;유영숙;최경화;임학태
    • Korean Journal of Plant Tissue Culture
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    • v.24 no.1
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    • pp.37-41
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    • 1997
  • The in vitro regeneration and genetic transformation systems in hot pepper(Capsicum annuum L.) have not been routinely available, which has been a major limiting factor in the application of new genetic manipulations. An efficient procedure to regenerate whole pepper plants and to generate transgenic plants expressing a foreign gene was established. A relatively high frequency of plant regeneration was observed when hypocotyl and cotyledon explants were cultured on MS medium supplemented with NAA 0.1 mg/L plus zeatin 2.0 mg/L or IBA 10.0 mg/L plus BAP 1.0 mg/L. Addition of AgNO$_3$5 $\mu$M to these media improved the regeneration frequency up to 8%. For plant transformation, hypocotyl and cotyledon explants of hot pepper were precultured on shoot induction media without kanamycin added for 2 days, and then cocultured with Agrobacterium tumefaciens pDY183 for 2 days. Putative transformants were obtained from selection media containing 100 mg/L kanamycin sulfate and 500 mg/L carbenicillin. Putatively selected transformants were confirmed by amplification of selectable marker genes (ADA and NPT II) by polymerase chain reacion. Successful transcripts of ADA gene were detected by Northern blot analysis. Enzyme activity of ADA was also examined by spectrophotometric analysis, and expression of ADA gene in hot pepper suggests the potential application of ADA gene as a selectable marker in plants.

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