• BMB Reports
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• v.40 no.5
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• pp.708-714
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• 2007

The two-component signal transduction, which typically consists of a histidine kinase and a response regulator, is used by bacterial cells to sense changes in their environment. Previously, the SphS-SphR histidine kinase and response regulator pair of phosphate sensing signal transduction has been identified in Synechocystis sp. PCC 6803. In addition, some response regulators in bacteria have been shown to be cross regulated by low molecular weight phosphorylated compounds in the absence of the cognate histidine kinase. The ability of an endogenous acetyl phosphate to phosphorylate the response regulator, SphR in the absence of the cognate histidine kinase, SphS was therefore tested in Synechocystis sp. PCC 6803. The mutant lacking functional SphS and acetate kinase showed no detectable alkaline phosphatase activity under phosphate-limiting growth conditions. The results suggested that the endogenous acetyl phosphate accumulated inside the mutants could not activate the SphR via phosphorylation. On the other hand, exogenous acetyl phosphate could allow the mutant lacking functional acetate kinase and phosphotransacetylase to grow under phosphate-limiting conditions suggesting the role of acetyl phosphate as an energy source. Reverse transcription PCR demonstrated that the transcripts of acetate kinase and phospho-transacetylase genes in Synechocystis sp. PCC 6803 is up-regulated in response to phosphate limitation suggesting the importance of these two enzymes for energy metabolism in Synechocystis cells

• Microbiology and Biotechnology Letters
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• v.31 no.2
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• pp.103-110
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• 2003

The regulation of pyrimidine nucleotide synthesis has been proved to be controlled by a regulatory protein PyrR-mediated attenuation in the Gram-positive bacteria. After several bacterial genome sequencing projects, we have discovered the PyrR orthologues in the databases for Haemophilus influenzae and Synechocystis and sp. PCC6803 genome sequences. To investigate whether these PyrR orthologue proteins regulate pyrimidine nucleotide synthesis as well as the cases of Bacillus, the PyrR regions of each strains were amplified by PCR and cloned with pUC19 or T-vector in Escherichia coli and with a shuttle vector pHPS9 for E. coli and B. subtilis. For the regulation test of the PyrR orthologues, the aspartate-transcarbamylase (ATCase) assay was carried out. From the results of the ATCase assay, it was confirmed that Synechocystis sp. PCC6803 could not restore by pyrimidines to a B. subtilis, PyrR but H. influenzae PyrR could. For Purification of PyrR orthologue proteins, PyrR orthologue genes were cloned into the expression vector (pET14b). Over-expressed product of PyrR orthologue genes was purified and analyzed by the SDS-PACE. The purified PyrR orthologue proteins from H. influenzae and Synechocystis sp. PCC6803 turned out to be molecular mass of 18 kDa and 21 kDa, respectively. The result of uracil phosphoribosyl transferase (UPRTase) assay with purified PyrR orthologue proteins showed that H. influenzae PyrR protein only has UPRTase activity. In addition, we could predict several regulatory mechanisms that PyrR orthologue proteins regulate pyrimidine de novo synthesis in bacteria, through phylogenetic analysis for PyrR orthologue protein sequences.

• Journal of the Korean Ceramic Society
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• v.53 no.1
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• pp.7-12
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• 2016

In this paper, we have developed a simple, new and economical carbonation method to synthesize a pure form of aragonite needles using dolomite raw materials. The obtained aragonite Precipitated Calcium Carbonate (PCC) was characterized by XRD and SEM, for the measurement of morphology, particle size, and aspect ratio (ratio of length to diameter of the particles). The synthesis of aragonite PCC involves two steps. At first, after calcinated dolomite fine powder was dissolved in water for hydration, the hydrated solution was mixed with aqueous solution of magnesium chloride at $80^{\circ}C$, and then $CO_2$ was bubbled into the suspension for 3 h to produce aragonite PCC. Finally, aragonite type precipitated calcium carbonate can be synthesized from natural dolomite via a simple carbonation process, yielding product with average particle size of $30-40{\mu}m$.

• Applied Chemistry for Engineering
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• v.9 no.7
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• pp.961-967
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• 1998

In order to improve the optical properties and printability of paper, various fillers are used in papermaking. This study was performed to investigate several fillers-precipitated calcium carbonate(PCC), ground calcium carbonate(GCC), and blends(PCC/talc or micro-talc(MVP) and GCC/talc or micro-talc(MVP)-for their effects on various newsprint properties. Results obtained from the study were summarized as follows ; 1) PCC treatment gave about 16.8% higher retention than GCC treatment at the filler level of 5% and the retention of filler in the handsheet increased as average particle size of mixed filler was increasing($PCC{\leq}GCC<MVP<talc$). 2) PCC treatment kept opacity more highly than no filler treatment and opacity decreased as average particle size of mixed filler was increasing. 3) Independent treatment of PCC kept tear strength more effectively than GeC did, and tear strength increased as average particle size of mixed filler was increasing. 4) Independent treatment of pee kept tensile strength more highly than other treatments and Gee treatment kept tensile strength highly than pee treatment under mixed filler treatment. 5) Burst strength under PCC treatments decreased linearly as average particle size of mixed filler was increasing. 6) Compared "With non-filler treatment, filler treatment gave much better printability.

• Journal of the Korean Society of Hazard Mitigation
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• v.9 no.1
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• pp.21-25
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• 2009

The results of a recent case study for material characterizations and structural evaluation to design asphalt overlay thickness of William P Hobby airport in Houston, Texas are presented herein. The existing runway 12R-30L of Hobby airport consisted of thick asphalt overlay over Portland Cement Concrete (PCC) and the localized surface shoving as evident in the closure of surface groove has been observed recently. Using the field cored asphalt concrete mixtures, measurements of percent air voids, asphalt content and aggregate gradation were conducted to find out the causations of surface shoving and groove closure. The FAA layered elastic program, LEDFAA was utilized to evaluate pavement structural conditions for new asphalt overlay. Two different composition assumptions for existing pavement were made to evaluate the pavement as followings: 1) APC, Asphalt Concrete Overlay over PCC pavement and 2) AC, Asphalt Concrete pavement. Based on laboratory testing results, a ratio of percent passing #200 to asphalt content ranged 1.1 to 2.2, which is considered a high ratio and a tendency of tender mix design was observed. Thus, the localized surface shoving and groove closure of the runway 12R-30L could be attributed to the use of excessive fine contents and tender mix design. Based on the structural evaluation results, it was ascertained that the analysis assuming the pavement structure as AC pavement gives more realistic structural life when the asphalt overlay is thicker enough compared to PCC layer because the existing PCC pavement under asphalt overlay acts more like a high quality base material.

• The Transactions of the Korean Institute of Power Electronics
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• v.20 no.1
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• pp.1-10
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• 2015

This paper proposes a control algorithm for permanent magnet synchronous generators with a back-to-back three-level neutral-point clamped voltage source converter in a medium-voltage off-shore wind power system under unbalanced grid conditions. Specifically, the proposed control algorithm compensates for unbalanced grid voltage at the PCC (Point of Common Coupling) in a collector bus of an off-shore wind power system. This control algorithm has been formulated based on symmetrical components in positive and negative synchronous rotating reference frames under generalized unbalanced operating conditions. Instantaneous active and reactive power is described in terms of symmetrical components of measured grid input voltages and currents. Negative sequential component of AC input current is injected into the PCC in the proposed control strategy. The amplitude of negative sequential component is calculated to minimize the negative sequential component of grid voltage under the limitation of current capability in a voltage source converter. The proposed control algorithm enables the provision of balanced voltage at the PCC resulting in the high quality generated power from off-shore wind power systems under unbalanced network conditions.

• Korean Chemical Engineering Research
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• v.55 no.4
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• pp.443-455
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• 2017

This article provides an exclusive overview of the synthesized aragonite precipitated calcium carbonate and its applications in various fields. The last decade has seen a steady increase in the number of publications describing the synthesis, characterization and applications of calcium carbonate morphologies. Mainly, two kinds of processes have been developed for the synthesis of aragonite precipitated calcium carbonate under controlled temperature, concentrations and aging, and the final product is single-phase needle-like aragonite precipitated calcium carbonate formed. This review is mainly focused on the history of developed methods for synthesizing aragonite PCC, crystal growth mechanisms and carbonation kinetics. Carbonation is an economic, simple and ecofriendly process. Aragonite PCC is a new kind of functional filler in the paper and plastic industries, nowadays; aragonite PCC synthesis is the most exciting and important industrial application due to numerous attractive properties. This paper describes the aragonite PCC synthetic approaches and discusses some properties and applications.

• Asian Journal of Turfgrass Science
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• v.10 no.4
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• pp.315-329
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• 1996

The changes of growth and protein synthesis pattern by aluminum (Al), cadmium (Cd), zinc (Zn) treatments were studied in Cyanobacterium synechocystis sp. PCC 6803. When exposed to Al from 5ppm to 3oppm, synechocystis grows normally. But more than that retard the growth of algae notably. The 0.05ppm Cd additions had no effect on the growth of algae. 0.1, 0.2, and 0.5ppm Cd inhibited growth. Under 1 and 2ppm Cd stress, growth was greatly diminished. Zn had dual effects. The growth of algae in media containing 5ppm was stimulated. As concentration increases more than l5ppm, growth inbition increases. Under 25ppm Zn stress, growth was greatly diminished. According to logistic theory, r and K values of each heavy metal-treated groups were estimated. Correlation analysis of r and K values with metal concentration shows that there is negative correlation between K and concentration in Cd and Zn treatments. Critical concentration which shows lethal or sublethal effect was estimated by t-test of each r and K value. The cells cultured in 10, 20, 30, 40 and 5oppm of Al, 1 and 2ppm of Cd, and 10, 15, 20, 25 and 30ppm of Zn for 4 days was used for protein analysis. Analysis of protein synthesis with SDS-PACE showed alterations of protein synthesis pattern. The synthesis of protein about 220kD increased markedly. In this study, it showed that resistance mechanism against Al, Cd, and Zn is K selection and that metal stress induced the change of protein synthesis in Cyanobacterium synechocystis sp. PCC 6803.Key words:Cyanobacterium synechocystis sp. FCC 6803, Heavy metals, Aluminum, Cadmiutm Zinc, Crowth, Frotein synthesis.

• MALSORI
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• no.61
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• pp.1-14
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• 2007

The purpose of this study was to analyze the relationship between segmental correctness and speech intelligibility in adults with hearing impairment. Segmental correctness was measured by percentage of correct vowels(PCV) and percentage of correct consonants(PCC). The results were shown as follows: First, PCV and PCC could predict speech intelligibility with statistical significance. Second, in consonant classes divided by place and manner of articulation, the PCC of plosives and alveolar sounds were significant predicting variables in each group ($R^{2}=50%;\;59%$). According to this study, the importance of segmental correctness on speech intelligibility of adults with hearing impairment was confirmed. Also correctness of plosive sounds in manner and alveolar sounds in place were significant factors to speech intelligibility.

• Proceedings of the Korean Society for Information Management Conference
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• 1998.08a
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• pp.205-208
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• 1998

1876년 Cutter의 개념을 계승한 AACR2R의 편목의 3수준은 오늘날과 같은 전자환경에서 유용하다. 본 연구에서는 편목의 다양한 수준에 관해 논의하였다. 최소수준목록(Minimal level Cataloging)은 영미에서 연구가 시작되어 미의회도서관과 OCLC에서 표준으로 제정하여 사용하고있으며, 핵심수준목록을 대표하는 PCC(Program for Cooperative Cataloging)의 핵심레코드(Core record) 표준은 최근들어 모든 수준의 도서관에서 사용이 증가되고 있다. 이에 인터넷자원의 표현을 위한 더블린코어와 PCC 핵심레코드의 데이터요소를 비교하여 공통데이터요소와 비공통데이터요소를 도출하여 인터넷환경에서 새로운 핵심수준 서지기술 데이터요소를 제안하는 기초자료로 삼고자 하였다.