• 한국미생물·생명공학회지
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• 제18권5호
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• pp.530-534
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• 1990

방선균의 한 종인 Thermoactinomuces sp. CH-53이 퇴비에서 분리되었다. 분리된 이 균은 pH 6.5-9.5, 수분 55-65의 무살균계분에서 왕성히 성장하며, 밀기울 배지에 포자를 충분히 착생시켜 시판의 배합사료에 1 비율로 첨가하여 닭에 급여해서 방선균의 생균수가$10^7-10^8$. cell/g에 도달하는 계분을 얻었으며, 처리 동안 악취성분이 소실되었다. 처리된 계분의 비료효과실험은 pot에서 Brassica rapa var. perviridis의 생육조사에서 다량시용에도 생육저해가 없었으며 질소함량으로 pot당 0.4g에 해당하는 시용구에서 최대생산을 보였다.

• 한국응용곤충학회지
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• 제32권1호
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• pp.51-60
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• 1993

흰 불나방 핵 다각체바이러스(Hyphantria cunea nuclear Palyhedrosis virus: HcNPV) 다각체 단백질 유전자포함 절편의 탐색과 클로닝을 행하였다. Autographa cahfornica NPV EeoRI-I 절편 (약 7.3 kb), Bombyx mori NPV PstI-F 절편 (약 7 kb) 및 합성 oligonucleotide ( 3D-mer) 를 probe로 한 southern hybridization을 행하여 HcNPV PstI - L 절편 (5.3 kb)을 탐색하고, pUC18을 이용하여 E. coli에 형질전환시켜 클로닝하였다. 클로닝한 plasmid의 EeoRI, SaIl, Kpnl, HindIII, SacI 및 AvaI의 제한효소지도를 작성하고 pHeP-L(8.0 kb)이라 명명히였으며, 이를 다시 pHcP-Ll(4.7 kb), pHcP-L2(7.1 kb), pHcP-L3(5.3 kb), pHcP-L4(4.2 kb) 및 pHeP-L5(4.5 kb)로 subeloning 하였다.

• 한국식품영양학회지
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• 제8권4호
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• pp.307-317
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• 1995

The purity of mtDNAs isolated from Archicitrus and Metacitrus leaves was higher in percoll density gradient centrifugation than differential and sucrose density gradient centrifugation. The most clear mtDNAs were obtained from mitochondria included in the Interface band of between 21% and 45% under isomotic, low viscosity conditions in the three step discontinuous percoll density gradient centrifugation. DNase treatment to the crude mitochondrlal suspension still more increased purity of mtDNA by the effective removal of the nuclear and chloroplast DNA and mtDNAs were appeared as a single band at middle position of tube by EtBr /cscl density gradient centrifugation. Agarose gel electrophoresis of mtDNAs resolved a single, broad band containing high molecular weight DNAs In all preparation. Yield of mtDNAs was about 110 and 2 ug Per 2009 in mature and immature leaves respectively. The mtDNA fragment patterns showed by EcoR I treatment were indistinguishable with respect to nom bet and position of bands in Archicitrus and Metacitrus. In the pattern of Hind E restriction, the Metacitrus displayed the unique band between 5.0 and 4.0kb, in addition to four fragments about 5.0, 2.4, 2.15, and 2.0kb, respectively, different from Archicitrus. Also the pattern of total mtDNAs fragment by the treatment of Pst I showed that the distinguishable fragment pat tern was not appeared in Archicitrus(C. iyo Tanaka), but about 6.0, 5.5, 5.0 and 2.Bkb fragments were appeared only in Metacitrus(C. junos Sieb). Therefore it was indicated that two species in intra-subgenus were identical each other, whereas considerable difference was revealed for inter-subgenus.

• 한국임상수의학회지
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• 제17권1호
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• pp.194-204
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• 2000

Echocardiography was performed to obtain the optimal planes for diagnosis of canine heartworm disease in 11 dogs. Imaging planes were taken the left ventricular outflow tract long-axis view, 4-chamber long-axis view, chordae tendineae level short-axis view and modified pulmonary arteries level short-axis view in the right parasternal window, and aptical 4-chamber long-axis view in hte left parasternal window. After echocaridography, the pulmonary artery valve pat was ligated with double 1-0 silk under the general anesthesia to prevent heartworm moving from pulmonary artery to right ventricle. The dogs were euthanized and examained for heartworms in heart and great vessels. Many adult heartworms were identified ultrasonographically within main pulmonary artery, bifurcation of pulmonary artery and right pulmonary artery, but not identified within right ventricle and atrium. At necropsy, adult heartworms were mainly found in pulmonary artery in 11 days, and also found in right ventricular outflow tract and right ventricle in 2 of 11 dogs. The modified pulmonary arteries level short-axis view was the best imaging plane for identifiying heartworms because the main pulmonary artery, branches of pulmonary artery and right pulmonary artery could be showed. The aortic root internal dimension (AOID) and right pulmonary atery internal dimension (RPAID) were measured from the modified pulmonary ateries level short-axis view and left ventricular outflow tract long-axis view. The RPAID and RPAID/AOID was higher in heartworm infected dogs than normal Korea Jin-do dogs in modified pulmonary arteries level short-axis view, and the AOID, RPAID and RPIAID/AOID was higher than in left ventricular outflow tract long-axis view, respectively. These results indicate that the pulmonary arteries were the major habitat of adult heartworm in canine heartworm disease and the modified pulmonary arteries level short-axis view was the best imaging planes for identifying heartworms because the main pulmonary arteries could be showed. Therefore the modified pulmonary arteries level short-axis view can be used for diagnosing heartworm disease and for monitoring dilation of pulmonary artery.

• 미생물학회지
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• 제4권2호
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• pp.5-10
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• 1966

The relationships between tumor score and peroxidase activities of tomato stems infected with Agrobacterium tumefaciens strain A6Kl, B6, T372 11BNV6, 11BV7 and wounded stem as a control were examined in relation to crown gall tumor development on purpose to study the lignification of tumor tissue which is affected to the development of crown gall tumor. As the previous paper has been mentioned the fact that the induction of tumor tissues were inhibited or limited in the lignified stem of host plant. It was presumed that the activities of peroxidase related to the development of lignification were decreased during the period of tumor development. But the experimental result in this experiment shows that the peroxidase activities of crown gall tumor-tissues infected with the A. tumefaciens strains which are already known as virulent are increasing during four weeks, however, in the strain 11BNV6 and wound the peroxidase activities are decreasing on the second week after the inoculation of the bacteria strains. These results could be explained on the basis of that possible regulatory agents of lignification which were accumulated in tumor tissues, IAA, ascorbic acid, glutathion(GSH) and caffeic acid esters, were postulated to act as antioxidants which has been suggested by Stafford. Total nitrogen contents in relation to crown gall tumor development were determined for the detection of protein synthesis related to the enzyme activities which are increasing in the time of plant growth. Generally six groups are contained the largest amount of nitrogen on the second week after the inoculation of the bacterium. Comparing to the tumor score, it is presumed that the all of enzyme activities including peroxidase in tumor tissues are increasing from the second week through the third week after the inoculation of bacterium and the protein synthesis is stimulated under the most appropriated temperature during the above periods.

• 대한미생물학회지
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• 제9권1호
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• pp.25-31
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• 1974

A comparative study was performed with 176 cultures of Salmonella organisms on tetracyline, neomycin and colistin in order to find out the relationship between the results obtained from the Ericsson's single disk method and the tube dilution method of antibiotic sensitivity tests which may be carried out in many hospital laboratories. With tetracycline, thirty-three out of 163 cultures of Salmonella typhi were found to be either sensitive or moderate sensitive by means of the disk method and thirty one(ca 94%) out of the thirty three cultures showed less than 1.0 ${\mu}g$ of the Minimal Inhibitory Concentretions(MIC) in the tube-dilution tests, which mean that there were a quite good agreement between the two methods. With neomycin, a hundred and five out of 163 S.typhi were appeared to be either sensitive or moderate sensitive by means of Ericsson's single disk method, among which 103 cultures showed less than 10.0 ${\mu}g$ MIC in the tubedilution method. And also there was a quite correlative pat. terns observed in the result of testing with 13 salmonella cultures other than S. typhi. With colistin, it was hard to observe any particular tendency in the distribution of plotting for 148 cultures showing less the 18 mm in the inhibiting zone diameters between MIC and disk sensitivity patterns except the fifteen, cultures out of 176 salmonella, which appeared to be sensitive in the single disk method and showed less than 1.0 ${\mu}g$ MIC in the tube dilution method.

• Archives of Pharmacal Research
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• 제27권9호
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• pp.947-953
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• 2004

The antioxidative and hepatoprotective potentials of two anthraquinones, alaternin (2-hydroxy-emodin) and emodin, to scavenge and/or inhibit hydroxyl radicals generated by the Fenton reaction and to protect tacrine-induced cytotoxicity in human liver derived HepG2 cells were evaluated, respectively. The inhibitory activity on hydroxyl radical generated in a cell-free chemical system (FeSO$_4$/$H_2O$$_2$) was investigated by a fluorescence spectrophotometer using a highly fluorescent probe, 2$^1$,7$^1$-dichlorofluorescein. The hydroxyl radical scavenging activity was determined by electron spin resonance spectroscopy using 5,5-dimethy-1-pyrroline-N-oxide as hydroxyl radicals trapping agents. Tacrine-induced HepG2 cell toxicity was determined by a 3-［4,5-dimethylthiazole-2yl］-2,5-diphenyltertrazolium bromide assay. Although the scavenging activity of alaternin on hydroxyl radical was similar to that of emodin in dose-dependent pat-terns, the inhibitory activity exhibited by the former on hydroxyl radical generation was stron-ger than that of the latter, with $IC_{50}$/ values of 3.05$\pm$0.26 $\mu$M and 13.29$\pm$3.20 $\mu$M, respectively. In addition, the two anthraquinones, alaternin and emodin showed their hepatoprotective activ-ities on tacrine-induced cytotoxicity, and the EC$_{50}$ values were 4.02 11M and 2.37 $\mu$M, respec-tively. Silymarin, an antihepatotoxic agent used as a positive control exhibited the EC$_{50}$ value of 2.00 $\mu$M. These results demonstrated that both alaternin and emodin had the simultaneous antioxidant and hepatoprotective activities.ies.

• Journal of Chest Surgery
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• 제33권1호
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• pp.20-25
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• 2000

Background: Median sternotomy remains the standard approach used by surgeons for most intracardiac operations but the residual scar is cosmetically unsatisfactory. To avoid an unsightly midline scar we have tried to use alternative skin incision (bilateral subm-ammary skin incision) to median sternal skin incision, In this study we have tried to compare different postoperative results of wound between two different skin incisional methods. Material and Method: Between June 1997 and June 1998, a bilateral subma-mmary skin incision combined with vertical sternotomy was performed in 21 pediatric female patients (submammary group)to repair acyanotic congenital heart disease. after the period we carried out a retrograde study about postoperative wound states comparing this incision with median sternal skin incision controls in whom there were 23 pediatric pat-ients (control group). Result: Patients' age ranged from 14 to 96 months(mean 38.2 months) Mean duration of subcutaneous drains using Hemovac which was used only in the patients of submammary group was 4.2 days and total amount of the drained effusion was 51.1 ml. Postoperative wound complications included wound eruption in one patient wound disruption in one patient and skin necrosis in 3 patients in submammary group and included wound disruption in 4 patients in controls. mean duration required for wound healing was 15,5 days in submammary group versus 10.4 days in controls. The mean scar length was 12.5 cm in submammary group versus 11.3 cm in controls. The average follow-up was 8.2 months in submammary group versus 9.0 months in controls. In submammary group 3 patients parents(14.3%) were pleased with their cosmetic results of wound scar but 8(38.1%) were dissatisfied. Among the 23 patients in control group 8(34.8%) were pleased but 8(34.8%) complained ofunhappiness with the scar.

• 대한치과보철학회지
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• 제37권5호
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• pp.672-686
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• 1999

The purpose of this study was to compare the casting problem and corrosion behavior in two types of HL Hexed abutments of the Steri-Oss system ; gold/plastic coping and gold coping. The anodic Polarization behavior, the galvanic corrosion between abutments and Type III gold alloys, before and after casting were analyzed, and the crevice corrosion of casting samples was analyzed with the CPPT test and the SEM. The results are as follows : 1. Anodic polarization behavior of samples ; Before casting, gold/plastic coping and gold coping was shown to have a similar corrosion pat-terns. Type III casting gold alloy was shown to have a lower corrosion potential and passivation film. Corrosion potential of the case of gold/plastic coping after casting was higher than that of gold coping, but the region of passivation film for gold/plastic coping was smaller than that of gold coping. 2. Galvanic corrosion behavior of samples ; Contact current density between casting gold alloys and gold/plastic before casting was higher than that between gold coping and casting gold alloy Galvanic corrosion of samples after casting was shown to have similar contact current density 3. Crevice corrosion behavior of samples ; Crevice corrosion resistance of casting sample using gold coping was lower than that of cast-ing sample using gold/plastic coping, and a severe corrosion pattern was observed at the abutment-casting gold alloy interface by the SEM.

• 한국항해항만학회:학술대회논문집
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• 한국항해항만학회 2000년도 Proceeding of CIN-KIN Joint Symposium 2000 on M.E.T. Under STCW 78/95 and SINO-KOREA MARITIME CONTACT IN MID-CENTURIES
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• pp.115-155
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• 2000

Nawadays LNG has been beginning to take the place of petroleum as fuel all over the world and VLCC tankers of LNG will take the same sea routes that had been used by VLCC tankers of petroleum in the last pat of he 20th century. The transportation of LNG by a VLCC include more dangerous nature of sea peril than that of petroleum. We already know the dimensions of a disaster a LNG tanker could bring about in the case of the LNG tanker, Yuyo-Maru No. 10 in the Tokyo Bay of Japan in 1974. From the point of safety when we construct a LNG base or LNG pier in the base, the appropriate government authority and constructing company had better take sea pilots or some ships handling experts to participate in a prior consultation of the design of the project. A G/T 100,000 ton LNG base and pier were completed in November of 1996 in Inchon harbour in Korea and LNG VLCC tankers of G/T 100,000 ton class have been entering into the base ever since. This study was started and completed to comply with the requisition of the Sea Pilot Association of Inchon harbour in advance of the opening of the LNG base. As the entrance and exit channels leading to Inchon harbour were constructed in the years of 1930s, it was one of the most pressing works for Inchon sear pilots in 1996 to certify the method of safe passing maneuvering of a G/T 100,000 ton LNG tanker through the Pudo narrow channel prior to commercing actual piloting of the LNG VLCC tanker. The author made some mathematical models computing maneuvering of a vessel changing her course with her control surface through a narrow channel and computed maneuvering of a G/T 100,000 ton LNG tanker and also made maneuvering simulations of the vessel by a desk-top simulator. The results of computations and simulations are well coincided with each other in qualitative aspects to assure safe passing of the LNG VLCC.