• Asian Pacific Journal of Cancer Prevention
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• v.15 no.24
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• pp.10647-10652
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• 2015

Background: The aim of our study was to establish COLD-PCR combined with an unlabeled-probe HRM approach for detecting KRAS codon 12 and 13 mutations in plasma-circulating DNA of pancreatic adenocarcinoma (PA) cases as a novel and effective diagnostic technique. Materials and Methods: We tested the sensitivity and specificity of this approach with dilutions of known mutated cell lines. We screened 36 plasma-circulating DNA samples, 24 from the disease control group and 25 of a healthy group, to be subsequently sequenced to confirm mutations. Simultaneously, we tested the specimens using conventional PCR followed by HRM and then used target-DNA cloning and sequencing for verification. The ROC and respective AUC were calculated for KRAS mutations and/or serum CA 19-9. Results: It was found that the sensitivity of Sanger reached 0.5% with COLD-PCR, whereas that obtained after conventional PCR did 20%; that of COLD-PCR based on unlabeled-probe HRM, 0.1%. KRAS mutations were identified in 26 of 36 PA cases (72.2%), while none were detected in the disease control and/or healthy group. KRAS mutations were identified both in 26 PA tissues and plasma samples. The AUC of COLD-PCR based unlabeled probe HRM turned out to be 0.861, which when combined with CA 19-9 increased to 0.934. Conclusions: It was concluded that COLD-PCR with unlabeled-probe HRM can be a sensitive and accurate screening technique to detect KRAS codon 12 and 13 mutations in plasma-circulating DNA for diagnosing and treating PA.

• Polymer(Korea)
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• v.38 no.5
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• pp.596-601
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• 2014

Polyamide (PA) oligomers, which are the hard segment of poly(ether-block-amide) (PEBA), presenting thermoplastic and high performance elastomeric properties were prepared by polycondensation between 4-aminobenzoic acid and 12-aminododecanoic acid. Subsequently PEBAs were obtained by addition polymerization of the PA oligomers and various molecular weights of poly(tetramethylene glycol) (PTMG). The structure of the final PEBA was identified by using FTIR and $^1H$ NMR and the thermal properties depending on changes in the structure of hard segment were collected by using DSC and UTM analysis. As the results, the melt temperature ($T_m$), the initial modulus, and the maximum strength of PEBAs increased with an increase in aromatic moiety up to 30% without reducing crystallinity.

• Journal of Microbiology and Biotechnology
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• v.12 no.3
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• pp.457-462
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• 2002

Low Electromagnetic Field (EMF) intensity in the range of $1{\mu}T\;to\;10{\mu}T$(Tesla) was found to enhance the growth of CHO cells and the production of tPA in batch and perfusion cultivations. At $1{\mu}T\;intensity,\;1.3{\times}10^7$ viable cells/ml of maximum cell density and 80 mg/l of maximum tPA production were obtained in batch cultivation, compared to $2.8{\times}10^6$ viable cells/ml and 59 mg tPA/1 in unexposed case (control). A similar trend was observed in the perfusion process, where it was possible to obtain $1.2{\times}10^7$ viable cells/ml of maximum cell density and 81 mg tPA/l of maximum tPA production by more than 80 days of cultivation. However, there was not much difference between $1{\mu}T\;and\;10{\mu}T$ in perfusion cultivation, possibly due to better environmental growth conditions being maintained by continuous feeding of fresh medium into the reactor. On the contrary, both cell growth and tPA production were severely inhibited at higher than 1 mT intensity, showing no growth at 10 mT exposure. Specific growth rate was linearly correlated to specific tPA production rate at $1{\mu}T$EMF intensity, which represents a partially growth-related relationship. It was also found that a large amount of $Ca^2+$ was released at low EMF intensity, even though the cell growth was not much affected. Low EMF intensity significantly improved both cell growth and tPA production, and tPA production seemed to be more affected than the cell growth, possibly due to the changes of cell membrane characteristics. It can be concluded that the elaboration of EMF intensity less than $10{\mu}T$ could improve cell growth and tPA production, but mainly tPA secretion through batch or perfusion process in a bioreactor.

• Pediatric Gastroenterology, Hepatology & Nutrition
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• v.17 no.4
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• pp.209-213
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• 2014

Purpose: Pathologic aerophagia (PA) may lead to bowel perforation or volvulus in mentally retarded patients. The authors investigated the effects of clonazepam on the management of PA in children with severe to profound mental retardation (MR). Methods: This study was undertaken as a retrospective case analysis of 21 PA patients with MR who were followed for over 12 months and diagnosed as having PA. Patients were assigned to two management groups, that is, to a clonazepam randomized open-labeled, treatment group or a reassurance group. The following were recorded and analyzed; age, response, remission rate to clonazepam treatment, and the side effect of clonazepam. It was defined positive response (response+) as being symptom-free for a whole week within 1 month of commencing treatment and remission(+) as being symptom-free for a whole month within 6 months of treatment. Results: The average age of the 21 PA children with MR was 10 years and 13 patients were female. Symptom duration before diagnosis of PA was 7 months. Clinical features of the clonazepam-trial group (n=11) and the reassurance group (n=10) were non-significantly different. Response(+) was achieved by 2 patients (18.2%) in the clonazepamtrial group and by no patient in the reassurance group. Remission(+) was achieved by 6 patients (54.5%) in the clonazepam-trial group and by one patient (10%) in the reassurance group (p=0.040). Conclusion: When PA children with MR with severe bowel distention are considered for surgical treatment to prevent acute abdomen, a trial of clonazepam could be recommended.

• Korean Journal of Exercise Nutrition
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• v.23 no.2
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• pp.16-21
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• 2019

[Purpose] The purpose of this study was to comparatively investigate the correlation among body composition, resting metabolic rate (RMR), and physical activity (PA) between young and middle-aged Korean adults. [Methods] A total of 53 [male n=23, female n=30] subjects were included in this study, among whom 34 subjects were healthy young adults [male n=18, female n=16] and 19 were middle-aged adults [male n=5, female n=14]. The body composition and RMR of all the participants were measured after overnight fasting (≥8 h). The Korean version of the WHO Global Physical Activity Questionnaire (GPAQ) was used to assess physical activity. [Results] Body composition was not significantly different between young adults and middle-aged adults. Whole-body bone mineral density and bone mineral contents (BMC) were significantly lower in middle-aged adults than in young adults. Total blood cholesterol (TC) and blood glucose levels were significantly higher in middle-aged adults (TC; 195.21 ± 43.34, glucose; 103.57 ± 12.61 mg/dL) than in young adults. RMR was significantly lower in middle-aged adults (1619.57 ± 290.28 kcal/day) than in young adults (1894.37 ± 405.00 kcal/day). In middle-aged adults physical activity (PA). PA (METs, min, EE) was inversely correlated with fat mass (FM, kg, and %) and blood triglyceride (TG) level in young adults. In middle-aged adults, PA showed a significant positive correlation with lean body mass (LBM), FM (%), and RMR. Furthermore, PA EE showed significant interrelatedness with BMC among middle-aged adults. [Conclusion] These results demonstrated that high PA levels enable LBM and RMR maintenance in middle-aged adults. Furthermore, in young adults, more PA is required to induce change in body composition.

• Tuberculosis and Respiratory Diseases
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• v.43 no.6
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• pp.987-996
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• 1996

Background : We prospectively evaluated the applicability and effect of noninvasive ventilation (NIV) in acute respiratory failure and tried to find out the parameters that could predict successful application of NIV. Methods : Twenty-six out of 106 patients with either acute ventilatory failure (VF: $PaCO_2$ > 43 mm Hg with pH < 7.35) or oxygenation failure (OF: $PaO_2/AO_2$ < 300 mm Hg with $pH{\geq}7.35$) requiring mechanical ventilation were managed by NIV (CPAP + pressure suppon, or BiPAP) with face mask. Eleven out of 19 cases with VF (57.9%) (M : F=7 : $55.4{\pm}14.6$ yrs) and 15 out of 87 cases with OF (17.2%) (M : F=12 : 3, $50.6{\pm}15.6$ yrs) were s uilable for NIY. Respiratory rates, arterial blood gases and success rate of NIV were analyzed in each group. Results: 81.8% (9/11) of YF and 40% (6/15) of OF were successfully managed on NIV and were weruled from mechanical ventilator without resorting to endotracheal intubation. Complications were noted in 2 cases (nasal skin necrosis 1, gaseous gastric distension 1). In NIV for ventilatory failure, the respiration rate was significantly decreased at 12 hour of NIV ($34{\pm}9$ /min pre-NIV, $26{\pm}6$ /min at 12 hour of NIV, p=0.045), while $PaCO_2$ ($87.3{\pm}20.6$ mm Hg pre-NIV, $81.2{\pm}9.1$ mm Hg at 24 hour of NIV) and pH ($7.26{\pm}0.04$, $7.32{\pm}0.02$, respectively, p <0.05) were both significantly decreased at 24 hour of NIV In NIV for oxygenation failure, $PaCO_2$ were not different between the successful and the failed cases at pre-NIV and till 12 hours after NIV. The $PaO_2/FIO_2$ ratio, however, significantly improved at 0.5 hour of NIV in successful cases and were maintained at around 200 mm Hg (n=6 : at baseline, 0.5h, 6h, 12h : $120.0{\pm}19.6$, $218.9{\pm}98.3$, $191.3{\pm}55.2$, $232.8{\pm}17.6$ mm Hg, respectively, p=0.0211), but it did not rise in the failed cases (n=9 : $127.9{\pm}63.0$, $116.8{\pm}24.4$, $100.6{\pm}34.6$, $129.8{\pm}50.3$ mm Hg, respectively, p=0.5319). Conclusion : From the above results we conclude that NIV is effective for hypercapnic respiratory failure and its success was heralded by reduction of respiration rale before the reduction in $PaCO_2$ level. In hypoxic respiratory failure, NIV is much less effective, and the immediate improvement of $PaO_2/FIO_2$ ratio at 0.5h after application is thought to be a predictor of successful NIV.

• Journal of the Korean Crystal Growth and Crystal Technology
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• v.32 no.1
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• pp.12-15
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• 2022

The effect of SiO₂, Na₂O and CaO on isokom temperatures in soda-lime glass is estimated by comparing calculated isokom temperatures using viscosity model proposed by Lakatos. The isokom temperatures at the viscosity of log η = 12.3, 10, 6.6 and 1 (Pa·s) are lowered by 6, 7, 10 and 24℃, respectively, by the substitution of SiO₂ with Na₂O by 1 mol%. Meanwhile, replacing 1 mol% of SiO₂ with CaO raises the isokom temperatures by 3~4 and 2℃ at the viscosity of log η = 12.3 and 10 (Pa·s), respectively, but lowers the temperatures by 1 and 21℃ at the viscosity of log η = 6.6 and 1(Pa·s), respectively.

• Journal of the Korean Catholic Hospital Association
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• v.9 no.2
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• pp.10-12
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• 1978

• The Zoological Society Korea : Newsletter
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• v.12 no.2
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• pp.112.1-112
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• 1995

No Abstract, See Full Text

• Journal of Chest Surgery
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• v.20 no.1
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• pp.30-38
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• 1987

Sixty-eight consecutive patients have been subjected to total corrective surgery of tetralogy of Fallot at the Hanyang University Hospital between August 1975 and December 1984. Of these, the pressure of chambers and great arteries were measured, before and immediately following an operation in 39 of the survived group and 11 of the expired group. The values of postoperative peak pressure were correlated with the outcome of the operation. The result are as followings; 1] Compared to the average peak pressure of right atrium in the survived group, 14.46*4.16 mmHg, that in the expired group, 19.36*8.19 mmHg, was significantly lower, 2] Compared to the average systolic pressure of right ventricle in the survived group, 56.72z16.37 mmHg, that in the expired group, 70.45*15.26 mmHg, was significantly lower. 3] Compared to the average systolic pressure of pulmonary artery in the survived group, 33.26*12.95 mmHg, that in the expired group, 37.55*11.63 mmHg, was higher. But the difference was not significant. 4] Compared to the average peak pressure of left atrium in the survived group, 17.41*6.90 mmHg, that in the expired group, 31.18*12.47 mmHg, was significantly higher. 5] Compared to the average systolic pressure of left ventricle in the survived group, 103.82~12.83 mmHg, that in the expired group, 90.55*20.02 mmHg, was significantly lower. 6] Compared to the average value of the right ventricle-pulmonary artery systolic pressure gradient [RV-PA] in the survived group, 24.95a15.44 mmHg, that in the expired group, 35.09*17.01 mmHg, was significantly higher. 7] Compared to the average value of the right ventricle to left ventricle systolic pressure ratio [RV/LV] in the survived group, 0.55*0.15, that in the expired group, 0.80a0.20, was significantly higher. 8] Compared to the average value of the pulmonary artery-left atrium peak pressure gradient [PA-LA] in 15.85x12.29 mmHg that in the expired group, 4.18x6.00 mmHg, was significantly lower. It is, therefore, suggested that in the operating room, PA-LA is as valuable as RV - PA, RV/LV in making important surgical decision and predicting the prognosis.