• Proceedings of the KSAR Conference
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• 2001.03a
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• pp.74-74
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• 2001

난자 동결방법의 선별은 보다 효과적인 난자은행의 개발에 필수 불가결한 중요한 요소이다. 이전의 연구에서 마우스의 난자를 ethylene glycol과 electron microscope grid를 이용한 유리화 동결법으로 동결 융해한 결과 기존의 slow freezing 방법에서보다 높은 생존율과 배발달율이 나타남을 관찰하였다. 그러나 동결융해후의 난자는 방추사와 염색체의 이상성이 대조군에 비해 높은 빈도로 나타나 융해후의 배발달율을 감소시키는 것으로 보고되었다. 이에 본 연구에서는 유리화동결법동안 항동해제에 Cytoskeleton system을 안정화시키는 cytoskeleton stabilizer인 taxol을 첨가시킨후 동결시켰을때 생존율과 발달율을 개선시킬 수 있는지 알아보고자 본 실험을 시행하였다. ICR mouse의 성숙란을 채취하여 연구목적에 따라 taxol을 첨가시키지 않은 대조군과 첨가시킨 실험군으로 분류하였다. 동결방법은 난자를 1.5 M ethylene glycol (EG)에 2분 30초간 노출시킨후 5.5 M EG와 1 M sucrose가 첨가된 동결액에 20초간 노출시킨 후 Grid에 난자를 부착시킨후 직접 액체질소에 침지하여 동결하였다. 동결후 난자는 5단계로 융해를 실시한 후 정자와 체외수정을 시킨 후 수정된 난자는 modified P1 배약액에 124 h까지 발달율을 관찰하였고, 배양 후 발달된 배반포는 대조군과 실험군, 각각 4마리의 발정동기화된 recipient에 이식을 시행하였다. 배발달율은 대조군에 비해 실험군에서 4세포기 (48 vs. 84.4%), 8세포기 (34% vs. 70.6%), 상실배 (26% vs. 58.6%) 그리고 배반포 발달율은 (24% vs. 58.6%)로 높게 관찰되었다. 배아이식후 대조군과 실험군에서 각각 2 마리가 임신이되어 정상적인 산자를 분만하였다. 따라서 항동해제에 taxol의 첨가는 동결 융해후의 난자의 배발달율을 증진시킬 수 있었다..8%로 나타나 난할율 및 배반포 발생율에 있어서 융합조건에 따라 큰 차이는 없었으나 1.9㎸/cm, 30$\mu\textrm{s}$ 2회의 조건이 다른 조건들에 비하여 유의적으로 낮았다. 따라서, 체세포와 수핵란 세포질간의 융합율과 배반포 발생에 미치는 영향은 전압보다는 시간에 더 크게 받음을 알 수 있었으며, 이와 같은 결과에서 융합시 시간을 오래 주는 것보다 전압을 높이는 것이 수핵난자의 세포질에 상해를 줄이고 이후 배반포 발생에 유리할 것으로 사료되었다.면에서도 더욱 더 활발할 것으로 기대된다. 배란후 72시간째에 초음파진단기를 이용하여 난소의 난포발달을 조사한 결과 , 대조구와 bFF처리구에 비해 AI처리구에서 발달난포가 유의적으로 많은 것을 확인하였다. 이상과 같은 결과로, Anti-inhibin serum은 한우 자체에서 분비하는 Inhibin을 특이하게 억제하여 Inhibin에 의해 억제되는 FSH분비가 촉진됨으로써 난포발달과 estrogen의 농도가 촉진되는 것으로 사료되어 anti-inhibin serum이 한우의 과배란유기 효과가 있는 것으로 사료된다.정량 분석한 결과이다. 시편의 조성은 33.6 at% U, 66.4 at% O의 결과를 얻었다. 산화물 핵연료의 표면 관찰 및 정량 분석 시험시 시편 표면을 전도성 물질로 증착시키지 않고, Silver Paint 에 시편을 접착하는 방법으로도 만족한 시험 결과를 얻을 수 있었다.째, 회복기 중에 일어나는 입자들의 유입은 자기폭풍의 지속시간을 연장시키는 경향을 보이며 큰 자기폭풍일수록 현저했다. 주상에서 관측된 이러한 특성은 서브스톰 확장기 활동이 자기폭풍의 발달과 밀접한 관계가 있음을 시사한다.se that were all low in two aspects, named "the Nonsignificant group". And the issues were high risk perception in general setting and

• Economic and Environmental Geology
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• v.31 no.2
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• pp.127-139
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• 1998

A new technique of simultaneous inversion for 3-D seismic velocity structure by using direct, reflected, and refracted waves is applied to the southeast part of the Korean Peninsula including Pohang Basin, Kyongsang Basin and Ryongnam Massif. Pg, Sg, PmP, SmS, Pn, and Sn arrival times of 44 events with 554 seismic rays are inverted for locations and crustal structure. $6{\times}6$ with $0.5^{\circ}$ and 8 layers (4 km each layer) model was inverted. 3-D seismic crustal velocity tomography including eight sections from surface to Moho, ten profiles along latitude and longitude are analyzed. The results are as follows: 1) the average velocity and thickness of sediment are 5.04 km/s and 3-4 km, and the velocity of basement is 6.11 km/s. The shape of velocity in shallower layer is agreement with Bouguer gravity anomaly (Cho et al., 1997). 2) the velocities fluctuate strongly in the upper crust. The velocity distribution of the lower crust under Conrad appears basically horizontal. 3) the average depth of Moho is 30.4 km, and velocity is 8.01 km/s. 4) from the velocity and depth of the sediment, the thickness, velocity and form of the upper crust, and the depth and form of Moho, we can find the obvious differences among Ryongnam Massif, Kyongsang Basin and Pohang Basin. 5) the deep faults (a Ulsan series faults) near Kyongju and Pohang areas can be found to be normal and/or thrust faults with detachment extended to the bottom of the upper crust.

• Journal of Periodontal and Implant Science
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• v.27 no.3
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• pp.585-595
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• 1997

Removal of subgingival calculus is essential for the success in periodontal treatment. Subgingival instrumentation is used for the removal of all bacterial plaque and calculus. In this study, two types of anterior curet were used on ant. teeth to conduct subgingival scaling and root planing. The remaining amount of calculus was evaluated according to type of instrument, depth of pocket, and tooth surface. 24 teeth extracted from patients being treated at Dan Kook University dept. Perio. were used. 4 surfaces per tooth a total of 96 areas were evaluated. 12 teeth treated with Gracey No. 1-2 was used as the control group and 12 teeth treated with Mini-five curet No. 1-2 was the experimental group. The 4 surfaces of the teeth {buccal, mesial, lingual or palatal, distal) were observed under a stereomicroscope and the images were captured 3 times per surfaced with a CCD. The image were observed on the monitor using a $10{\times]10$ grid produced with the Microsoft power point. The amount of calculus remaining was evaluated 3 times per surface. The results were as follows. 1. There was no significant difference in remaining calculus according to the pre-treatment pocket depth, and tooth position{Mx. or Mn). 2. The Mini-five curet showed better results than the Gracey curet but there was no statistically significant difference. 3. In both Gracey curet group and Mini-five curet group the lingual(or palatal) surface showed significant difference compared to the other surfaces(p<0.05). From the results above, it is thought that when treating ant. teeth consideration of the tooth surface is more important than the choice of instrument.

• Journal of the Korean Society of Food Science and Nutrition
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• v.41 no.2
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• pp.270-277
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• 2012

The purpose of this study was to evaluate the importance and satisfaction level foreigners place on human and physical evidence service in Korean restaurants based on nationality such as American/European, Japanese, Chinese, and Southeast Asian. The research was performed by using questionnaires conducted from August to September on 365 foreigners living in Busan. Total mean scores for the importance (3.96/5.00) and satisfaction (3.33/5.00) of Korean restaurant human and physical evidence service were significantly different (p<0.01), as the gap was -0.63. The mean scores of the gap were -1.03 for cleanliness of dining area, -1.01 for cleanliness of tableware, and -0.95 for easily understandable menu board. The mean scores of satisfaction for Southeast Asian individuals (3.77) were significantly (p<0.01) higher than those of American/European (3.40), Chinese (3.37), and Japanese (2.81). The importance and satisfaction grid showed that an easily understandable menu board and a brief description of the food or menu scored high for the importance and low for the satisfaction in American/European, Chinese, and Japanese individuals. Cleanliness of the dining area, cleanliness of tableware, cleanliness of staff appearance, kindness of employees, rapid resolution of complaints, and prompt and quick service scored high for importance and low for satisfaction in Japanese. However, Southeast Asians were satisfied with human and physical evidence service of Korean restaurants.

• The Transactions of the Korean Institute of Electrical Engineers P
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• v.64 no.2
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• pp.57-61
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• 2015

Current research and development efforts and other projects for energy storage systems (ESS) have recently been gaining attention. This is due to the many applications where ESS, particularly batteries, can be used. Among these are peak shaving, frequency regulation, and stabilization of renewable energy output. KEPCO has completed the construction and demonstration of a 4-MW battery energy storage system (BESS) located in Jeju City to verify its practicability in the power grid. KEPCO Research Institute has also been developing technology for the commercialization of BESS, and has been conducting a trial run of a 52-MW ESS (28MW + 24MW of Seo-Anseong and Shin-Yongin substations) constructed in 2014 for frequency regulation. This paper discusses the development of operation methods, as well as an operation user interface, for the safe operation and monitoring of BESS used for frequency regulation in a power system. Included are operation and simulation methods for various normal and transient frequency situations that can be experienced by a power system. Also discussed are the results obtained after applying these methods to the 4-MW BESS and the 52-MW ESS, both used for frequency regulation. The technology in this paper will be applied to 500MW ESS for frequency regulation of KEPCO by 2017. It is expected that this technology helps a safe and reliable operation and control of ESS for frequency regulation through its continuous upgrade.

• Journal of the Korean Society of Food Science and Nutrition
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• v.43 no.12
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• pp.1920-1928
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• 2014

The purpose of this study was to evaluate the importance and performance of middle school foodservice hygiene in Busan. In this study, questionnaires were administered to 1,000 students consisting of 100 students in 10 middle schools in Busan in December, 2013. The students assessed importance and performance of personal hygiene as 3.81 and 3.60 on a scale of 5.00, respectively. Males gave higher scores to importance and performance of personal hygienic than females. First grade students gave significantly higher scores than 2nd and 3rd grade students to importance and performance of personal hygienic. The mean scores of importance of foodservice hygiene were 4.47 for food hygiene, 4.25 for equipment hygiene, 4.23 for employee hygiene, and 4.19 for environment hygiene. For importance of foodservice hygiene, females gave significantly (P<0.001) higher scores than males. However, male gave significantly higher scores than females for performance of foodservice hygiene. The importance and performance grid showed important factors for personal and foodservice hygiene. 'Hand washing before eating the meal' for personal hygiene and 'cleanliness of tray' for foodservice hygiene showed the highest importance and lowest performance scores. Foodservice hygiene can be improved through customized education of students regarding meal service and food hygiene, equipment hygiene, and environment hygiene.

• Journal of the Korean Dietetic Association
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• v.19 no.3
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• pp.265-286
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• 2013

The present study was conducted to develop a standardized job description for clinical dietitians working in hospitals. A developing curriculum (DACUM) method was used for the job analysis of clinical dietitians. Based on DACUM analysis with 14 members, including clinical dietitians and professors majoring in clinical nutrition and job analysis, information on the duties, tasks, and task elements of clinical dietitians was determined. To verify the job descriptions derived from DACUM analysis, a total of 46 tertiary and general hospitals with over 500 beds were recruited for the survey. The final developed job description for clinical dietitians included 7 duties, 27 tasks, and 93 task elements. The duties consisted of nutritional assessment, nutrition diagnosis, nutrition intervention, nutrition monitoring evaluation, consultation cooperation, nutrition research, and self-development. The mean scores of perceived importance, performance, and difficulty on the clinical dietitian's task elements (out of a maximum score of 5.0) were 4.5, 3.7, and 3.5, respectively, with significant differences between the items (P<0.001). The perceived importance and performance grid of clinical dietitian's tasks showed that "construction and maintenance of collaboration" (E2) and "activity of quality improvement" (F1) received relatively low scores for performance despite their high importance scores; thus the performance of these tasks requires significant improvement. In conclusion, the job descriptions of clinical dietitians developed from this study are useful for the qualitative improvement of clinical nutrition services in hospitals.

• KOREAN JOURNAL OF CROP SCIENCE
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• v.50 no.4
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• pp.221-237
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• 2005

For developing the site-specific fertilizer management strategies of crop, it is essential to know the spatial variability of soil factors and to assess their influence on the variability of crop growth and yield. In 2002 and 2003 cropping seasons within-field spatial variability of rice growth and yield was examined in relation to spatial variation of soil properties in the· two paddy fields having each area of ca. $6,600m^2$ in Suwon, Korea. The fields were managed without fertilizer or with uniform application of N, P, and K fertilizer under direct-seeded and transplanted rice. Stable soil properties such as content of clay (Clay), total nitrogen (TN), organic mater (OM), silica (Si), cation exchange capacity (CEC), and rice growth and yield were measured in each grid of $10\times10m$. The two fields showed quite similar spatial variation in soil properties, showing the smallest coefficient of variation (CV) in Clay $(7.6\%)$ and the largest in Si $(21.4\%)$. The CV of plant growth parameters measured at panicle initiation (PIS) and heading stage (HD) ranged from 6 to $38\%$, and that of rice yield ranged from 11 to $21\%$. CEC, OM, TN, and available Si showed significant correlations with rice growth and yield. Multiple linear regression model with stepwise procedure selected independent variables of N fertilizer level, climate condition and soil properties, explaining as much as $76\%$ of yield variability, of which $21.6\%$ is ascribed to soil properties. Among the soil properties, the most important soil factors causing yield spatial variability was OM, followed by Si, TN, and CEC. Boundary line response of rice yield to soil properties was represented well by Mitcherich equation (negative exponential equation) that was used to quantify the influence of soil properties on rice yield, and then the Law of the Minimum was used to identify the soil limiting factor for each grid. This boundary line approach using five stable soil properties as limiting factor explained an average of about $50\%$ of the spatial yield variability. Although the determination coefficient was not very high, an advantage of the method was that it identified clearly which soil parameter was yield limiting factor and where it was distributed in the field.

• Korean Journal of Community Nutrition
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• v.16 no.5
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• pp.602-612
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• 2011

The purpose of this study was to evaluate importance and performance of dietitian's task at long term care hospitals foodservices in the Busan Kyongnam area. The research was performed through using questionnaires and conducted from June 11 to July 16, 2010 for 186 dietitians at 141 long-term care hospitals. Seventy-two percent of hospitals had two dietitians and 69% of them had a dietitian's office. Fifty-two percent of dietitians has worked for less than 2 years at long term care hospital, and 37.1% of them worked additional tasks. Seventy-three percent of hospitals conducted a therapeutic diet program and the therapeutic diets frequently provided were diabetic diet > tube feeding diet > dysphasia diet > sodium controlled diet. Mean score for the importance (4.36/5.00) and performance (3.91/5.00) of dietitian's tasks were significantly different (p < 0.001). The importance and performance grid showed that the purchase-inspection management and sanitation-safety management were high scores to the importance and performance (doing great area), menu-foodservice management and cooking-working management were low scores to the importance and high scores to the importance (overdone area), and nutrition management was low scores to the importance and performance (low priority). Forty-three percent of dietitians agreed with the needs for role separation between foodservice dietitian and clinical dietitian.

• Journal of The Korean Society of Agricultural Engineers
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• v.55 no.1
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• pp.17-29
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• 2013

Long-Term Hydrologic Impact Assessment (L-THIA) model which is a distributed watershed model was applied to analyze the spatial distribution of surface runoff and nonpoint source pollutant loading from Imha watershed during 2001~2010. L-THIA CN Calibration Tool linked with SCE-UA was developed to calibrate surface runoff automatically. Calibration (2001~2005) and validation (2006~2010) of monthly surface runoff were represented as 'very good' model performance showing 0.91 for calibration and 0.89 for validation as Nash-Sutcliffe (NS) values. Average annual surface runoff from Imha watershed was 218.4 mm and Banbyun subwatershed was much more than other watersheds due to poor hydrologic condition. Average annual nonpoint source pollutant loading from Imha wateshed were 2,295 ton/year for $BOD_5$, 14,752 ton/year for SS, 358 ton/year for T-N, and 79 ton/year for T-P. Amount of pollutant loading and pollutant loading rates from Banbyun watershed were much higher than other watersheds. As results of analysis of loading rate from grid size ($30m{\times}30m$), most of high 10 % of loading rate were generated from upland. Therefore, major hot spot area to manage nonpoint source pollution in Imha watershed is the combination of upland and Banbyun subwatershed. L-THIA model is easy to use and prepare input file and useful tool to manage nonpoint source pollution at screening level.