• Asian-Australasian Journal of Animal Sciences
• /
• 제18권9호
• /
• pp.1242-1248
• /
• 2005

This study was designed to investigate the in vitro developmental ability and apoptosis of bovine embryos nucleartransferred (NT) with frozen-thawed or cooled donor cells. Cultured adult bovine ear cells were used as donor cells after sub-culturing to confluence (CC), cooling to 4$^{\circ}C$ for 48 h, or freezing-thawing (FT). Apoptotic cells in blastocysts were evaluated for apoptosis by terminal deoxynucleotidyl transferase mediated dUTP nick end labeling (TUNEL) method. Fusion, cleavage and blastocyst rates were 69.0 (167/242), 68.8 (115/167), and 29.9 (50/167) with CC cells, 70.4 (88/125), 69.3 (61/88), and 29.6 (26/88) with cooled cells and 66.1 (117/177), 70.1 (82/117), and 13.7 (16/117) with FT cells, respectively. Blastocyst rates of NT embryos derived from FT cells were significantly lower than those from CC or cooled cells (p<0.05). In addition, NT blastocysts produced by using FT cells showed significantly higher apoptosis rates (6.4${\pm}$4.0%) than those produced by CC (2.8${\pm}$1.7%) or cooled (2.3${\pm}$1.3%) cells. However, cooling of donor cells had no significant adverse effect on blastocyst rate as well as apoptosis rate. Therefore, our results suggest that cooled cells may be used as an alternative to freshly cultured confluent culture cells, as donor cells, for the production of Somatic nuclear cloned cattle.

• 한국수산과학회지
• /
• 제53권3호
• /
• pp.342-350
• /
• 2020

The objective of this study was to investigate the sensory and nutritional characteristics of seasoned broughton's ribbed ark Scapharca broughtonii product (S-BRA). Based on 100 g, the proximate composition of S-BRA was 68.9 g moisture, 16.1 g crude protein, 4.0 g crude lipid and 4.0 g ash. The salinity was 2.2 g. Based on the results of a taste-intensity test using an electronic tongue, S-BRA had a stronger umami taste and less sourness than the control (BRA prepared with commercial seasoning sauce) (P<0.05). In a sensory evaluation, S-BRA scored higher for appearance, taste, flavor, and texture compared to the control. The total amino acid content based on 100 g of S-BRA was 16.04 g, and the major amino acids were aspartic acid, glutamic acid, leucine, and arginine. The S-BRA was higher in calcium, phosphorus, potassium, iron, and zinc than the control. The fatty acid content based on 100 g of S-BRA was 3,242 mg, and the major fatty acids were 16:0, 20:5n-3 and 22:6n-3. These results indicated that S-BRA had greater nutritional value than the control.

• Molecular & Cellular Toxicology
• /
• 제3권1호
• /
• pp.53-59
• /
• 2007

This study was conducted to evaluate the mutagenic potential of dimethyl isophthalate (DMIP) using Ames bacterial reverse mutation test, chromosomal aberration test and mouse lymphoma $tk^{+/-}$ gene assay. As results, in Ames bacterial reversion assay, DMIP was tested up to the concentration of 5,000 ${\mu}g$/plate and did not induce mutagenicity in Salmonella typhimurium strains TA98, TA100, TA1535 and TA1537, and Escherichia coli WP2uvrA with or without metabolic activation (S9 mix). Using cytotoxicity test, the maximal doses of DMIP for chromosomal aberration assay were determined at 1,250 ${\mu}g/mL$, which was a minimum precipitation concentration ($IC_{50}>1,940\;{\mu}g/mL$ or 10 mM) and at 155 ${\mu}g/mL$ ($IC_{50}:155\;{\mu}g/mL$) in the presence and the absence, respectively, of S9 mix. DMIP in the presence of S9 mix induced statistically significant (P<0.001) increases in the number of cells with chromosome aberrations at the dose levels of over 250 ${\mu}g/mL$, when compared with the negative control. However, DMIP in the absence of S9 mix did not caused significant induction in chromosomal aberrant cells. In MLA, DMIP at the dose range of 242.5-1,940 ${\mu}g/mL$ in the presence of S9 mix induced statistically significant increases in mutation frequencies related to small colony growth, whereas any significant mutation frequency was not observed in absence of S9 mix. From these results, it is conclusively suggested that dimethyl isophthalate may be a clastogen rather than a point mutagen.

• Journal of Preventive Medicine and Public Health
• /
• 제51권5호
• /
• pp.242-247
• /
• 2018

Objectives: To examine survivorship disparities in demographic factors and risk status for non-muscle-invasive bladder cancer (NMIBC), which accounts for more than 75% of all urinary bladder cancers, but is highly curable with early identification and treatment. Methods: We used the US National Cancer Institute's Surveillance, Epidemiology, and End Results registries over a 19-year period (1988-2006) to examine survivorship disparities in age, sex, race/ethnicity, and marital status of patients and risk status classified by histologic grade, stage, size of tumor, and number of multiple primary tumors among NMIBC patients (n=29 326). We applied Kaplan-Meier (K-M) and Cox proportional hazard methods for survival analysis. Results: Among all urinary bladder cancer patients, the majority of NMIBCs were in male (74.1%), non-Latino white (86.7%), married (67.8%), and low-risk (37.6%) to intermediate-risk (44.8%) patients. The mean age was 68 years. Survivorship (in median life years) was highest for non-Latino white (5.4 years), married (5.4 years), and low-risk (5.7 years) patients (K-M analysis, p<0.001). We found significantly lower survivorship for elderly, male (female hazard ratio [HR], 0.96), Latino (HR, 1.20), and unmarried (married HR, 0.93) patients. Conclusions: Survivorship disparities were ubiquitous across age, sex, race/ethnicity, and marital status groups. Non-white, unmarried, and elderly patients had significantly shorter survivorship. The implications of these findings include the need for a heightened focus on health policy and more organized efforts to improve access to care in order to increase the chances of survival for all patients.

• Asian-Australasian Journal of Animal Sciences
• /
• 제15권11호
• /
• pp.1611-1616
• /
• 2002

The alkanes in plant materials can potentially be used as markers to estimate composition and digestibility of diet of deer for the development of feeding strategies, but the analysis of alkanes in plant materials and deer faeces is time-consuming and expensive. In this study, 242 faecal samples and 119 pasture samples were scanned using near infrared spectroscopy (NIR) and the concentrations of alkanes in these samples were analysed to develop calibrations for predicting alkane concentrations in pastures and deer faeces. The $R^2$ values for NIR calibrations were <0.6 for $C_{24}$, $C_{26}$, $C_{32}$ and $C_{36}$, but were >0.8 for other alkanes for faecal samples. The $R^2$ values were >0.87 for alkanes with chains from $C_{27}$ to C35 for pasture samples. However, NIR was unable to predict concentrations of alkanes with chains of $C_{24}$, $C_{26}$, $C_{32}$ and $C_{36}$ in faecal samples and $C_{24}$, C25, $C_{26}$ and $C_{36}$ in pasture samples. While the use of these NIR calibrations will accelerate the estimation of diet digestibility, dietary components and botanical composition of pastures, the influence of the type of pasture on NIR calibration will require further examination.

• Asian Pacific Journal of Cancer Prevention
• /
• 제16권15호
• /
• pp.6645-6650
• /
• 2015

Background: Gastric cancer (GC) is the third most common cancer regarding mortality in the world. The cag pathogenicity island (PAI) of Helicobacter pylori which contains genes associated with a more aggressive phenotype may involve in the pathogenesis of gastrointestinal disease. We here aimed to examine the associations of cagH, cagL, orf17, and cagG genotypes of H. pylori cag PAI with severe gastrointestinal disease. Materials and Methods: A total of 242 H. pylori strains were genotyped. Histopathological examination and classification of subjects were performed. Results: The frequencies of the cagH, cagL, cagG, and orf17 genotypes were 40/54 (74.1%), 53/54 (98.1%), 38/54 (70.4%), and 43/54 (79.6%), respectively, in patients with peptidic ulceration (PU),while in the control group, the frequencies were 87/147 (59.6%) for cagH, 121/146 (82.9%) for cagL, 109/146 (74.7%) for cagG, and 89/146 (61.0%) for orf17. The results of simple logistic regression analysis showed that the cagL and orf17 genotypes were significantly associated with an increased risk of PU not GC; the ORs (95% CI) were 10.950 (1.446-82.935), and 2.504 (1.193-5.253), respectively. No significant association was found between the cagH and cagG genotypes and the risk of both the PU and the GC in Iran (P>0.05). Finally, multiple logistic regression analysis showed that the cagL genotype was independently and significantly associated with the age-and sex-adjusted risk for PU; the OR (95% CI) was 9.557 (1.219-17.185). Conclusions: We conclude that the orf17 and especially cagL genotypes of H. pylori cag PAI could be factors for risk prediction of PU, but not GC in Iran.

• Safety and Health at Work
• /
• 제2권3호
• /
• pp.236-242
• /
• 2011

Objectives: To determine the feasibility of predicting static and dynamic peak back-compressive forces based on (1) static back compressive force values at the lift origin and destination and (2) lifting speed. Methods: Ten male subjects performed symmetric mid-sagittal floor-to-shoulder, floor-to-waist, and waist-to-shoulder lifts at three different speeds (slow, medium, and fast), and with two different loads (light and heavy). Two-dimensional kinematics and kinetics were captured. Linear regression analyses were used to develop prediction equations, the amount of predictability, and significance for static and dynamic peak back-compressive forces based on a static origin and destination average (SODA) backcompressive force. Results: Static and dynamic peak back-compressive forces were highly predicted by the SODA, with R2 values ranging from 0.830 to 0.947. Slopes were significantly different between slow and fast lifting speeds (p < 0.05) for the dynamic peak prediction equations. The slope of the regression line for static prediction was significantly greater than one with a significant positive intercept value. Conclusion: SODA under-predict both static and dynamic peak back-compressive force values. Peak values are highly predictable and could be readily determined using back-compressive force assessments at the origin and destination of a lifting task. This could be valuable for enhancing job design and analysis in the workplace and for large-scale studies where a full analysis of each lifting task is not feasible.

• 한국치위생학회지
• /
• 제20권2호
• /
• pp.233-242
• /
• 2020

Objectives: The purpose of this study is to investigate the relationship between perceived oral health, medical service satisfaction, and oral health impact profile (OHIP-14) and the factors affecting OHIP for orthodontic patients, and to provide basic data to improve the quality of life of orthodontic patients in accordance with oral health. Methods: The study explained the purpose and purport of this study to orthodontic patients who visit dental clinics located in Daejeon and Chungcheong province for about two months from July 01, 2019, and conducted a self‐reported questionnaire survey for 220 participants who agreed to participate in this study. of the collected questionnaires, data of 197 subjects were used for the final analysis except 23 that were inadequate. Results: As for orthodontic treatment satisfaction according to perceived oral health and OHIP, there was statistically significant difference between respondents who were 'very healthy' and 'not healthy' (p<0.05). The higher the supplementary service satisfaction, the higher the orthodontic treatment satisfaction, and the higher the perceived oral health, the OHIP was found to be the higher. The factors affecting OHIP were found to be significantly associated with orthodontic treatment satisfaction and perceived oral health. The explanatory power was 15.6%. Conclusions: Active and ongoing oral health education should be provided to dental hygienists to ensure that the patient's oral cavity is healthy, and although additional services need to be improved, it is more important to have management strategies for patients to improve orthodontic treatment satisfaction.

• 한국환경농학회지
• /
• 제11권3호
• /
• pp.235-242
• /
• 1992

부식산과 흘브산이 Paraquat의 약해에 미치는 영향은 호밀의 생물검정을 통해 고사일수, 생중량 및 성장률에 관한 약해지표를 설정하고 Model을 도입하여 평가했다. 호밀의 생육상황에 대한 유기리간드 자체의 영향은 거의 없었으나, Paraquat의 독성은 매우 높았다. Paraquat의 약해가 강하게 나타나기 시작하는 농도는 $0.4{\sim}0.6{\mu}M$ 이었으며, 약해한계농도 (Phytotoxic Critical concentration)는 약 11${\mu}M$ 로 추정되었다. 유기리간드의 처리는 Paraquat의 약해에 의해 초래되는 고사일수를 약 40%까지 지연 시켰으며, Paraquat 단독처리구보다 생중량 및 성장률을 유의성있게 향상시켜, Paraquat의 호밀에 대한 약해를 줄여 주었다.

• Asian-Australasian Journal of Animal Sciences
• /
• 제29권1호
• /
• pp.29-35
• /
• 2016

The aim of the present study was to investigate the effect of single nucleotide polymorphisms in the major histocompatibility complex (MHC) class II gene on resistance to Newcastle disease virus and body weight of the Thai indigenous chicken, Leung Hang Khao (Gallus gallus domesticus). Blood samples were collected for single nucleotide polymorphism analysis from 485 chickens. Polymerase chain reaction sequencing was used to classify single nucleotide polymorphisms of class II MHC. Body weights were measured at the ages of 3, 4, 5, and 7 months. Titres of Newcastle disease virus at 2 weeks to 7 months were determined and the correlation between body weight and titre was analysed. The association between single nucleotide polymorphisms and body weight and titre were analysed by a generalized linear model. Seven single nucleotide polymorphisms were identified: C125T, A126T, C209G, C242T, A243T, C244T, and A254T. Significant correlations between log titre and body weight were found at 2 and 4 weeks. Associations between single nucleotide polymorphisms and titre were found for C209G and A254T, and between all single nucleotide polymorphisms (except A243T) and body weight. The results showed that class II MHC is associated with both titre of Newcastle disease virus and body weight in Leung Hang Khao chickens. This is of concern because improved growth traits are the main goal of breeding selection. Moreover, the results suggested that MHC has a pleiotropic effect on the titre and growth performance. This mechanism should be investigated in a future study.