• Reproductive and Developmental Biology
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• v.29 no.3
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• pp.141-144
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• 2005

This experiment was to investigate whether the mitochondria function assessment can be used for the prediction of sperm fertility through examining the correlation between mitochondria fluoromicroscopic frequency of frozen-thawed eight Hanwoo bull semen using rhodamine123 (R123) and in vitro embryo development following fertilization. Individual sperm were stained in 5 ${\mu}g/mL$ R123-added calcium-free Sp-TALP for 30 min at 0 h, 6 h, 12 h and 24 h after thawing and examined their mid-piece under an epifluorescence microscope using 495 nm excitation filter (x1,000). Three replications were taken, and at least 300 sperm per individual were examined. When semen samples were separated into two groups (good and poor) by sperm motility and fluorescent frequencies at just after thawing, average fluorescent frequencies were remarkably reduced as time going (0 h; $53.29{\~}72.94\%$, 6 h; $21.40{\~}58.90\%$, 12 h; $8.26{\~}25.93\%$, 24 h; $1.00{\~}13.78\%$, irrespective of selected group, and there were no differences at 6 h or 12 h after thawing between selected groups but indicated significant difference at 24 h after thawing (p<0.05). In vitro fertilization rates in good and poor groups ranging $70.8{\~}77.8\%$ and $52.1{\~}84.5\%$, respectively, were not significantly different. However, in vitro development rates of the same groups ranging $25.7{\~}40.0\%$ and $12.9{\~}1.8\%$, respectively, were significant different (p<0.05). These results demonstrate that mitochondria fluoromicroscopic assessment of frozen-thawed bovine sperm may be used as a criterion to select more fertile sperm.

• Childhood Kidney Diseases
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• v.20 no.2
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• pp.88-91
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• 2016

Nephrogenic diabetes insipidus (NDI) can cause nonobstructive hydronephrosis. Congenital NDI (CNDI) is caused by a genetic mutation. This case report presents a 12-year-old girl who was incidentally diagnosed with nonobstructive hydronephrosis due to NDI caused by AQP2 gene mutation after being evaluated for microscopic hematuria found on routine health examination at school. The patient's medical and family history was unremarkable, and she complained of nocturia only at the time of the clinic visit. Bilateral hydronephrosis on abdominal ultrasonography prompted a water deprivation test, leading to diagnosis of NDI. Genetic study confirmed p.Asn (AAC)123Ser (AGC) in exon 2 of the AQP2 gene. Polyuria and hydronephrosis improved following arginine-vasopressin therapy. CNDI responsive to treatment should be considered as a possible cause of nonobstructive hydroureter.

• The Journal of the Korean dental association
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• v.22 no.10 s.185
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• pp.869-877
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• 1984

An objective examination of 123 subjects aged from 19 to 23 was made from a clinical standpoints to determine the natural occurrence in anterior tooth contact in centric occlusion, and tooth contact in protrusive occlusion, left and right lateral excursions, and temporomandibular dysfunction. 1. In centric occlusion, maxillary 6 anterior contact type was frequently observe (30.08%) compared with other types of contact (incisor contact: 19.47%, no contact: 17.70%, canine and incisor contact: 15.04%, canines contact: 12.39%, unilateral canine contact: 5.31%) (P<0.01) 2. In protrusive position (edge to edge bite), maxillary central incisors contact was predominant (86.7%). (P<0.01) 3. In lateral excursion, there was not any significant difference between canine guided occlusion (47.79%) and group function occlusion (total 51.32%, AG:9.29%, PG:13.27%, G:28.76%). 4. Temporomandibular joint dysfunction was observed in 12.4% of 123 subjects.

• Journal of Life Science
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• v.13 no.5
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• pp.712-717
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• 2003

In order to establish yeast cells that produce leucocin A, a bacteriocin, the 117 bp leucocin A gene with start and stop codons was synthesized and cloned in pAUR123, a yeast vector. Transformed yeast cells showed antibacterial activity against Bacillus subtilis. The leucocin A gene was confirmed by means of PCR methods with plasmid prepared from transformed yeast cells as template and two leucocin A-specific primers. In this results, yeast cells that produce mass amounts of bacteriocin to use as food preservative or antibiotics were established.

• Journal of Environmental Science International
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• v.4 no.5
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• pp.123-123
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• 1995

The treatment performances of anaerobic-aerobic activated sludge process were investigated under various operation conditions. The treatment system proposed in this study gave a relatively stable performance against hourly change of the flow rate and showed a satisfactory removal of nitrogen and phosphorus compounds under experimental conditions. The recycle ratio of mixed liquor from aerobic to anaerobic region and peak coefficient primarily controlled the extent of nitrogen removal. The recycle ratio had the optimum values which were determined by the microbial activities of nitrification and denitrification. The behavior of the treatment unit could be simulated by using the kinetic equations and reactor models which considered the treatment units as complete mixing tanks.

• Advances in Traditional Medicine
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• v.10 no.2
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• pp.123-133
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• 2010

The entire plant of Leptadenia reticulata (Asclepidaceae) is extensively used as lactogen, traditionally, in veterinary practice. The plants of Dregea volubilis and Pentatropis microphylla (Asclepidaceae) are now used as its substitute and sometimes replace the original drug as traditional lactogen. The lactogenic potential of these drugs was studied in rats using, pup weight, weight of mother, parenchyma percentage, secretary rating, estimation of total protein content and glycogen content of mammary glands tissues as assessment parameters. HPTLC profiles of bioactive extracts were also generated to serve the authentification needs. The results of present studies show that P.microphylla forms a better substitute over D. volubilis.

• Animal Systematics, Evolution and Diversity
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• v.32 no.2
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• pp.123-132
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• 2016

Six species of springtails (Collembola) are reported for the first time from North Korea: Pachyotoma takeshitai (Kinoshita, 1916), Folsomia bisetosa Gisin, 1953 and Agrenia agilis Fjellberg, 1986 of Isotomidae, Entomobrya sibirica Stach, 1963 and Willowsia buski (Lubbock, 1870) of Entomobryidae, and Ptenothrix setosa (Krausbauer, 1898) of Dicyrtomidae. The genus Pachyotoma Bagnall, 1949 is mentioned for the first time from the Korean Peninsula. In order to facilitate the identification of these species, illustrations and short diagnoses are provided and the poorly known P. takeshitai is described in detail. Ptenothrix setosa and E. sibirica link the Korean peninsular fauna with Central Asia, P. takeshitai shows the zoogeographical connection of the Korean Peninsula with the Japanese Islands and A. agilis has a trans-pacific distribution. Folsomia bisetosa is an interesting circumpolar element in the Korean fauna while W. buski is a subcosmopolitan species.

• Proceedings of the Korea Environmental Mutagen Society Conference
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• 2003.10a
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• pp.123-123
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• 2003

• Proceedings of the Korean Society of Toxicology Conference
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• 2003.10b
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• pp.123-123
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• 2003

Primary cultures of rat fetus brain exhibit phenotypes of neuron, oligodendrocyte, and astrocyte from "neurospheres". To understand the role of mitogen-activated protein kinase (MAPK) cascade and gap junctional intercellular communication (GJIC) in the differentiation of neurosphere-derived astrocyte, we investigated the effects of 12-O-tetradecanoylphorbol-13-acetate (TPA) on the cultured astrocyte morphology.(omitted)

• The Korean Journal of Nuclear Medicine
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• v.29 no.1
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• pp.105-109
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• 1995

L-3-[$^{123}I$]iodo-${\alpha}$-methyltyrosine([$^{123}I$] IMT) was synthesized by electrophilic radio-iodination using chloramine-T and Iodobead in phosphate buffered solution. And the biodistribution was examined in 9L glioma bearing rats. The radiosynthesis of [$^{123}I$]IMT with iodobead was simpler and higher in radiochemical yield(88%) than the method using chloramine-T(83%) as radioiodinating reagent. The highest yield was obtained from the reaction using 1 piece of Iodobead, $200{\mu}g$ ${\alpha}$-methyltyrosine in $100{\mu}l$ phosphate-buffered solution(pH 5.5) and the reaction was completed in 7min. 24 hours after the injection, the biodistribution in 9L glioma transplanted rats revealed the in vivo deiodination, the excretion via kidney, and 3 times higher uptake in the tumor than normal brain. These results suggest the promising clinical use of [$^{123}I$] IMT in the various malignancies.