• Journal of Microbiology and Biotechnology
• /
• v.19 no.3
• /
• pp.257-264
• /
• 2009

A $\beta$-agarase gene, agaB34, was functionally cloned from the genomic DNA of a marine bacterium, Agarivorans albus YKW-34. The open reading frame of agaB34 consisted of 1,362 bp encoding 453 amino acids. The deduced amino acid sequence, consisting of a typical N-terminal signal peptide followed by a catalytic domain of glycoside hydrolase family 16 (GH-16) and a carbohydrate-binding module (CBM), showed 37-86% identity to those of agarases belonging to family GH-16. The recombinant enzyme (rAgaB34) with a molecular mass of 49 kDa was produced extracellularly using Escherichia coli $DH5{\alpha}$ as a host. The purified rAgaB34 was a $\beta$-agarase yielding neoagarotetraose (NA4) as the main product. It acted on neoagarohexaose to produce NA4 and neoagarobiose, but it could not further degrade NA4. The maximal activity of rAgaB34 was observed at $30^{\circ}C$ and pH 7.0. It was stable over pH 5.0-9.0 and at temperatures up to $50^{\circ}C$. Its specific activity and $k_{cat}/K_m$ value for agarose were 242 U/mg and $1.7{\times}10^6/sM$, respectively. The activity of rAgaB34 was not affected by metal ions commonly existing in seawater. It was resistant to chelating reagents (EDTA, EGTA), reducing reagents (DTT, $\beta$-mercaptoethanol), and denaturing reagents (SDS and urea). The E. coli cell harboring the pUC18-derived agarase expression vector was able to efficiently excrete agarase into the culture medium. Hence, this expression system might be used to express secretory proteins.

• The Journal of the Society of Korean Medicine Diagnostics
• /
• v.16 no.2
• /
• pp.59-70
• /
• 2012

Objectives : Pulse diagnosis by Expert is the only way to classify 8 Constitutions so the study to supplement classifying method by the questionnaire has developed and modified and ECM-32 System has designed in 2010. But analyzing with Decision tree had many nodes and 32 important questions omitted while processing the data. So this study was to classify the 8 constitution patients into 2 groups first and analyze its characters in consecutive order. Methods : The participants of this study were 1027 patients who classified into one of the 8 constitutions according to pulse diagnosis and answered 251 questionnaires in 2010. They were divided into sympathetic nerve acceleration constitution and parasympathetic nerve acceleration constitution and analyzed with decision tree. Results : The reponses of the questionnaire were analyzed with 4 methods of 5 scales interval method from 0 to 5, Na, Low(1,2), Medium(3), High(4,5), average value, Y/N dichotomy. Average Value had no significance. 1. From the 5 scale interval method 6 questionnaires with 7 nodes (F5e, B1d, F7f, F2a, F1b, C4L) were significant. The accuracy was 92.5%. 2. From L, M, H method 7 questionnaires with 7 nodes(F5e, B1d, F7f, F1a, B1c, C4L, P3d) were significant. The accuracy was 92.5%. 3. From Y/N dichotomy 9 questionnaires with 9 nodes( F5e, B1d, F7f, F1a, B1c, C4L, B1b, P1i, B2a) were significant. The accuracy was 93.18%. Conclusions : Based on this study, Yes or No dichotomy method was most significant and categorized among the 4 methods. Unlike previous studies which used interval scale method only, Y/N dichotomy method was more statistically significant with the questionnaire to supplement the method of pulse diagnosis. For further study by analyzing decision tree method in consecutive order, the patients can be divided into 8 Constitutions with higher significance with less questionnaires.

• The Transactions of the Korean Institute of Electrical Engineers P
• /
• v.63 no.2
• /
• pp.61-68
• /
• 2014

Function of battery bank stores energy for DC load in general, and DC power system of the nuclear power plant is used to supply DC loads for safety- featured instrumentation and control such as inverter, class 1E power system control and indication, and station annunciation. Class 1E DC power system must provide a power for the design basis accident conditions, and adequate capacity must be available during loss of AC power and subsequent safe shutdown of the plant. In present, batteries of Class 1E DC power system of the nuclear power plant uses lead-acid batteries. Class 1E batteries of nuclear power plants in Korea are summarized in terms of specification, such as capacity, discharge rate, bank configuration and discharge end voltage, etc. This paper summarizes standards of determining battery size for the nuclear power plant, and analyzes duty cycle for the class 1E DC power system of nuclear power plant. Then, battery cell size is calculated as 2613Ah according to the standard. In addition, this paper analyzes performance test results during past 13 years and shows performance degradation in the battery bank. Performance tests in 2001 and 2005 represent that entire battery cells do not reach the discharge-end voltage. Howeyer, the discharge-end voltage is reached in 14.7% of channel A (17 EA), 13.8% of channel B (16 EA), 5.2% of channel C (6 EA) and 16.4% of channel D (19 EA) at 2011 performance test. Based on the performance test results analysis and size calculation, battery capacity and degradation by age in Korearn nuclear power plant is discussed and would be used for new design.

• Proceedings of the KSME Conference
• /
• 2001.06d
• /
• pp.565-571
• /
• 2001

The objective of this study was to investigate the characteristics of air flow and heat transfer caused by trapezoid rods array in impinging air jet system. In this study, trapezoid rods have been set up on front of flat plate to act as a turbulence promoter. Local Nusselt numbers were determined as a function of three parameters: (a) the space from rods to heating surface(C=1, 2, 4mm), (b) the pitch between each rods(P=30, 40, 50mm), (c) the distance from nozzle exit to flat plate(H/B=2, 6, 10). And this research compared the above with the experiment without trapezoid rods. As a result, heat transfer performance was best under the condition of C=1mm and as the pitch is 30mm. In this case, maximum rate of heat transfer augmentation is about 1.9 times greater compared to that without trapezoid rods.

• Proceedings of The Korean Society of Agricultural and Forest Meteorology Conference
• /
• 2016.09a
• /
• pp.41-43
• /
• 2016

• Journal of Microbiology and Biotechnology
• /
• v.33 no.3
• /
• pp.371-377
• /
• 2023

In this study, a pepA gene encoding glutamyl (aspartyl)-specific aminopeptidase (PepA; E.C. 3.4.11.7) was cloned from Tetragenococcus halophilus CY54. The translated PepA from T. halophilus CY54 showed very low similarities with PepAs from Lactobacillus and Lactococcus genera. The pepA from T. halophilus CY54 was overexpressed in E. coli BL21(DE3) using pET26b(+). The recombinant PepA was purified by using an Ni- NTA column. The size of the recombinant PepA was 39.13 kDa as determined by SDS-PAGE, while its optimum pH and temperature were pH 5.0 and 60℃, respectively. In addition, the PepA was completely inactivated by 1 mM EDTA, indicating its metallopeptidase nature. The Km and Vmax of the PepA were 0.98 ± 0.006 mM and 0.1 ± 0.002 mM/min, respectively, when Glu-pNA was used as the substrate. This is the first report on PepA from Tetragenococcus species.

• Journal of Microbiology
• /
• v.38 no.2
• /
• pp.80-87
• /
• 2000

The nucleotide sequence of the luxC gene coding for lux-specific fatty acyl-CoA reductase and the upstream DNA (325bp)of the structural gene from bioluminescent bacterium, Photobacterium phosphoreum, has been deternubed. An open reading frame extending for more than 20 codons in 325 bp DNA upstream of luxC was not present in both directions. The lux gene can be translated into a polypeptide of 54 kDa and the amino acid sequences of lux specific reductases of P. phosphoreum shares 80, 65, 58, and 62% identity with those of the Photobacterium leiognathi, Vibrio fischeri, Vibrio harveyi, and Xehnorhabdus luminescenens reductases, respectively. Analyses of codon usage, showing that a high frequency (2.3%) of the isoleucine codon, AUA, in the luxC gene compared to that found in Escherichia coli genes (0.2%) and its absence in the luxA and B genes, suggested that the AUA codon may play a modulator role in the expression of lux gene in E. coli. The structural genes (luxC, D, A, B, E) of the P. phosphoreum coding for luciferase (${\alpha}$,${\beta}$) and fatty acid reductase (r, s, t) polypeptides can be expressed exclusively in E. coli under the T7 phage RNA polymerase/promoter system and identificationof the [35S]methionine labelled polypeptide products. The degree of expression of lux genes in analyses of codon usage. High expression of the luxC gene could only be accomplished in a mutant E. coli 43R. Even in crude extracts, the acylated acyl-CoA reductase intermediate as well as acyl-CoA reductrase activities could be readily detected.

• Journal of dental hygiene science
• /
• v.12 no.2
• /
• pp.123-129
• /
• 2012

The aim of study was to compare the color parameters and mean color difference of porcelain specimens by different veneering technique in order to examine the effect of veneering technique on esthetics of yttria-stabilized tetragonal zirconia polycrystalline(Y-TZP) all ceramic restoration. Three groups of square-shaped core ceramic specimens(14mm in diameter and 0.3, 0.5, 0.7 mm) and two groups of veneering ceramic specimen were prepared for analysis. Color parameter($L^*,a^*,b^*$) and color difference of zirconia core squares and core-veneer specimens were measured with ShadeEye $NCC^{(R)}$ spectrophotometer, respectively. Mean color difference(${\Delta}E^*$)were calculated using color difference formula. Two-way analysis of variance(ANOVA) combined with a Tukey multiple-range test were used to analysis the data(${\alpha}=0.05$). ${\Delta}E^*$ values were not significantly affected by core thickness and veneering porcelain(p=0.083). The color differences(${\Delta}E^*$) of core-veneer specimens with 0.5, 0.7 mm-A1,A2,A3.5 shade were mostly below 3.7 which was within the clinically acceptable range, while color differences between 0.3 mm-A1,A2 showed more than 3.7. All-ceramic system has color characteristics that clinicians have to consider when selecting materials. Also, manufacturers of different porcelain systems must make every effort to achieve color reproducibility.

• Asian-Australasian Journal of Animal Sciences
• /
• v.12 no.6
• /
• pp.871-880
• /
• 1999

The effects of dry roasting (110, 130, $150^{\circ}C$ for 15, 30, 45 min) on potential ruminant protein nutritional values in terms of: a), rumen bypass protein (BCP); b), rumen bypass starch (BST); c), fermented organic matter (FOM); d), true absorbed bypass protein (ABCP); e) microbial protein synthesized in the rumen based on available energy (E_MP); f), microbial protein synthesized in the rumen based on available nitrogen (N_MP); g), true protein supplied to the small intestine (TPSI); h), true absorbed rumen synthesized microbial protein (AMP); i), endogenous protein losses (ENDP); j), true digested protein in the small intestine (DVE); k), degraded protein balance (OEB) of whole lupin seeds (WLS) and faba beans (WFB) were evaluated by the new Dutch DV/OEB protein evaluation system. Dry roasting significantly increased BCP, BST, TPSI, ABCP, DVE (p<0.001) and decreased FOM, E_MP, AMP, N_MP and OEB (p<0.001) with increasing temperatures and times except that when temperature was at $110^{\circ}C$. The values of BCP, BST, TPSI, ABCP and DVE at $150^{\circ}C/45min$ for WLS and WFB were increased 2.2, 3.7; -, 2.0; 1.7, 1.7; 2.3, 3.7 and 1.7, 1.7 times and the values of FOM, E_MP, AMP, N_MP and OEB at $150^{\circ}C/45min$ for WLS and WFB were decreased by 15.3, 25.8; 18.1, 25.8; 18.7, 25.8; 54.6, 41.6 and 82.3% 54.7%, respectively, over the raw WLS and WFB. The results indicated that though dry roasting reduced microbial protein synthesis due to reducing FOM, TPSI didn't decrease but highly increased due to increasing BCP more than enough for compensation of the microbial protein decreasing. Therefore the net absorbable DVE in the small intestine was highly increased. The OEB values were significantly reduced for both WLS and WFB but not to the level of negative. It indicated that microbial protein synthesis might not be impaired due to the sufficient N supplied in the rumen, but the high positive OEB values in the most treatments except of $150^{\circ}C$ for 30 and 45 min of WLS (The OEB values: 54.8 and 26.0 g/kg DM) indicated that there were the large amounts of N loss in the rumen. It was concluded that dry roasting at high temperature was effective in shifting protein degradation from rumen to intestines and it increased the DVE values without reaching the negative OEB values. No optimal treatment was found in WLS due to the too high OEB values in all treatments. But dry roasting at $150^{\circ}C$ for 30 and 45 min might be optimal treatments for WLS due to the very lower OEB values.

• ETRI Journal
• /
• v.28 no.2
• /
• pp.257-259
• /
• 2006

In this letter, we will evaluate the performance degradation of a 40 km high-speed (40 Gb/s) optical system, induced by optical fiber variations of the chromatic dispersion induced by temperature changes. The chromatic dispersion temperature sensitivity will be estimated based on the signal quality parameters.