• 한국식품영양과학회지
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• 제42권8호
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• pp.1318-1324
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• 2013

본 연구는 경남지역에서 5개의 산업체 공장 위탁급식소 근로자 300명을 대상으로 설문조사를 통해 급식서비스 만족도와 고객충성도를 평가하고, 급식서비스 만족도가 고객충성도에 미치는 영향을 파악함으로써 산업체 위탁급식의 경쟁력을 높이는데 필요한 자료를 제공하고자 시도되었다. 설문조사는 2012년 7월 20일~2012년 8월 31일까지 진행하였다. 연구 결과, 급식서비스 만족도 평가는 전체 평균 점수가 3.51/5.00점으로 나타났으며, 음식 3.47/5.00점, 서비스 3.50/5.00점, 위생 3.65/5.00점, 환경 3.45/5.00점이었다. 이용기간에 따른 급식서비스 만족도는 2년 이상~4년 미만이 가장 높은 점수를 보였으나 유의적인 차이는 보이지 않았다. 직무에 따른 급식서비스 만족도는 생산직이 사무직보다 유의적(P<0.001)으로 높은 점수를 보였으며, 음식(P<0.001), 서비스(P<0.001), 위생(P<0.01), 환경(P<0.001) 요인의 각 급식서비스 만족도도 생산직이 사무직보다 유의적으로 높은 점수를 보였다. 고객충성도 전체 평균 점수는 3.46/5.00점이며 재구매 의도 3.60/5.00점, 구전 의도 3.52/5.00점, 전환하지 않을 의도 3.27/5.00점이었다. 이용기간은 전환하지 않을 의도 중 '비록 나는 시간과 돈이 허락해도 외부 식당을 이용하지 않을 것이다'는 2년 이상~4년 이하가 2년 미만과 4년 이상보다 유의적(P<0.05)으로 높은 점수를 보였다. 생산직이 사무직보다 구전 의도(P<0.01), 전환하지 않을 의도(P<0.001)에서 유의적으로 높은 점수가 나타났다. 급식서비스 요인과 고객충성도와의 상관관계는 음식, 서비스, 위생, 환경 요인 모두 재방문 의도, 구전 의도, 전환하지 않을 의도와 양의 유의적인(P<0.01) 상관관계를 보였다. 재방문 의도는 음식 요인이, 구전 의도와 전환하지 않을 의도(P<0.01)는 서비스 요인이 다른 요인보다 유의적으로(P<0.01) 높은 상관관계를 보였다. 고객충성도에 영향력을 주는 급식서비스 만족도 요인 분석에서, 재구매 의도에는 위생, 음식, 서비스요인의 순으로, 구전 의도에는 서비스, 음식, 위생요인 순으로, 전환하지 않을 의도에는 서비스, 음식요인의 순으로 영향력을 주어 산업체 위탁급식소에서 고객충성도의 세 가지 요인에 영향력을 모두 주는 급식 서비스요인은 서비스요인과 음식요인이었다.

• 한국미생물·생명공학회지
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• 제20권5호
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• pp.519-526
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• 1992

Bacillus pasteurii의 urease gene이 Escherichia coli HB101에서 발현된 Bacillus성 recombinant urease를 단일단백질으로 정제하고 그 효소학적 특성을, 별도로 정제한 B.pasteurii urease의 그것과 비교검토하였다. B.pasteurii urease gene이 cloning 된 E.coli HB101(pBU11)의 균체파쇄액으로 부터 TEAE-cellulose, DEAE-Sephadex A-50, Sephadex G-150, sephadex G-200 등의 이온교환 크로마토그래피와 gel filtration을 이용하여 E.coli내에서 발현된 B. pasteurii성제하였으며, 또한 B.pasteurii로부타 비활성도 185.2배의 urease를 정제하여 disc gel electrophoresis로 단일 단백으로 정제되었음을 확인하였다. 정제된 두 urease 들의 native 상태의 분자량은 공히 280,000$pm$10,000 정도로 확인되었고, SDS-electrophoresis에의 해 subunit 유무와 분자량을 확인한 결과도 67,000정도의 subunit 4개와 20,000의 subunit 1개로 된 $\alpha$$\beta$ 구조의 동일한 효소단백으로 추정할 수 있었다. Gene donor인 B. pasteurii와 cloning 된 균주 E. coli(pBU11)이 생산한 두 urease의 효소학적 특성을 비교 조사해본 결과 두 urease의 최적반응 pH는 공히 7.5로 나타났으며, pH에 대한 안정성도 두 ureaserk 공히 pH 5.5에서 10.5 사이에서 50% 이하로 활성이 떨어지지 않는 강한 pH 안정성을 보였다. 두 urese의 최적반응의 온도는 $60^{\circ}C$였으며, 비교적 온도에 대한 저항이 강한 효소임을 알았다. 두 urease의 활성에 미치는 금속이온의 영향은 $Ag^{2+}$, $Hg^{2+}$ 등에서 양효소가 모두 강한 저해현상을 받는 반면, $Mn^{2+}$, $Mg^{2+}$ 에서는 다소 촉진되는 현상을 보였다. 효소반응 저해제들의 영향을 조사해 본 결과 p-CMB, acetohydroxamic acid에 두 urease가 모두 강한 저해를 받았다. 두 urease의 $K_m$ 값과 $V_{max}$ 값은 E. coli(pBU11)의 urease는 $4.21{\times}10^{-2}mol/\ell$, $86.96\ell$mol/min 이었고, B. pasteurii urease는 $4.04{\times}10^{-2}mol/\ell$, $160\ell$mol/min이었다. 따라서 B. pasteurii의 urease나 그 urease gene으로 cloning되어 E. coliso에서 발현된 recombinant urease는 분자량이나 효소학적 특서에서 거의 동일한 효소단백임을 알 수 있었다.

• Journal of Chest Surgery
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• 제47권4호
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• pp.344-349
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• 2014

Background: The Damus-Kaye-Stansel (DKS) procedure is a method for mitigating the risk of systemic ventricular outflow tract obstruction (SVOTO). However, there have been few reports on which surgical technique shows a better outcome. The objective of this study was to compare the outcome of the DKS procedure according to the surgical technique used. Methods: We retrospectively reviewed 12 consecutive patients who underwent the DKS procedure from March 2004 to April 2013. When the relationship of the great arteries was anterior-posterior, the double-barrel technique (group A) was performed. If the relationship was side-by-side, the ascending aortic flap technique (group B) was performed. Results: There was no early mortality and 1 late mortality in group B. There was no statistically significant difference in the median peak pressure gradient of preoperative subaortic stenosis in both groups: 14 mmHg (range, 4 to 53 mmHg) in group A and 15 mmHg (range, 0 to 30 mmHg) in group B (p=0.526). Further, a significant postoperative pressure gradient was not observed in either group A or group B. More than moderate postoperative neoaortic regurgitation was observed in 1 patient of group B; this patient underwent neoaortic valve replacement 66 months after the DKS procedure. No one had a recurrent SVOTO during follow-up. Conclusion: The DKS procedure is an effective way to minimize the risk of SVOTO, and there is little difference in the outcomes of the DKS procedure according to the surgical technique used.

• Journal of Nutrition and Health
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• 제44권6호
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• pp.518-526
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• 2011

To determine blood heavy metal concentrations by seafood consumption in Korean adults, blood cadmium, mercury, and lead concentrations in a representative sample of 1,709 Koreans participated in the Fourth Korea National Health and Nutrition Examination Survey (KHANES IV-2) in 2008 were analyzed by age and seafood consumption frequency. The mean blood cadmium, mercury, and lead concentrations were $1.14{\pm}0.73{\mu}g/L$, $5.50{\pm}3.83{\mu}g/L$, and $2.56{\pm}1.22{\mu}g/dL$, respectively. The subjects aged ${\geq}$ 50 years had significantly higher blood cadmium concentrations than the subjects aged 20~39 years. Blood mercury concentrations of the 50's were significantly higher than those of the 20's and 30's (p < 0.05). Approximately, 43% of males and 22% of females had blood mercury concentrations > $5.8{\mu}g/L$ which is a blood mercury level equivalent to the current Reference Dose. Only 2 subjects had lead concentrations > $10{\mu}g/dL$, the standard lead level by the Centers for Disease Control and Prevention, USA. The subjects consuming pollack, mackerel, anchovy, corvina, shellfish, and salted seafood at least once a week had significantly higher mercury concentrations than the subjects consuming those seafoods less than once a month. However, there were no clear relationships between blood cadmium and lead concentrations and seafood consumption frequencies.

• 한국축산식품학회지
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• 제40권4호
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• pp.512-526
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• 2020

Synthetic nitrite is considered an undesirable preservative for meat products; thus, controlling synthetic nitrite concentrations is important from the standpoint of food safety. We investigated 1,000 species of microorganisms from various kimchi preparations for their potential use as a starter culture for the production of nitrites. We used 16S rRNA gene sequence analysis to select a starter culture with excellent nitrite and nitric oxide productivity, which we subsequently identified as Staphylococcus hominis subspecies hominis WiKim0113. That starter culture was grown in NaCl (up to 9%; w/v) at 10℃-40℃; its optimum growth was observed at 30℃ at pH 4.0-10.0. It exhibited nonproteolytic activity and antibacterial activity against Clostridium perfringens, a bacterium that causes food poisoning symptoms. Analysis of Staphylococcus hominis subspecies hominis WiKim0113 with an API ZYM system did not reveal the presence of β-glucuronidase, and tests of the starter culture on 5% (v/v) sheep blood agar showed no hemolytic activity. Our results demonstrated the remarkable stability of coagulase-negative Staphylococcus hominis subspecies hominis WiKim0113, especially in strain negative for staphylococcal enterotoxins and sensitive to clinically relevant antibiotics. Moreover, Staphylococcus hominis subspecies hominis WiKim0113 exhibited a 45.5% conversion rate of nitrate to nitrite, with nitrate levels reduced to 25% after 36 h of culturing in the minimal medium supplemented with nitrate (200 ppm). The results clearly demonstrated the safety and utility of Staphylococcus hominis subspecies hominis WiKim0113, and therefore its suitability as a starter culture.

• 한국치위생학회지
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• 제10권2호
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• pp.335-344
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• 2010

Objectives : Targeting Korean adults, the food pattern are grasped. And, its correlation with oral disease is analyzed. In order to offer basic data to developing the nutritional policy and nutritional program for the future prevention from oral disease, a research was conducted by utilizing the Korean National Health and Nutrition Examination Survey 2005(the 3rd term). Methods : The subjects in this study were 6,526 adults in more than fully 19 years among 9,047 persons who participated in the food intake survey out of those who completed the health interview survey. The statistical analysis was analyzed by using SPSS 12.0 program. Results : 1. As a result of Group Analyzing was indicated to dangerous-type food pattern and protection-type food pattern. 2. As a result of analyzing the answers for having dental caries in the annually personal recognition was indicated to be high in the dangerous-type food pattern, and had not the statistically significant difference. 3. As a result of analyzing the answers for having periodontal disease in the annually personal recognition was indicated to be high in the dangerous-type food pattern, and had the statistically significant difference(p<0.05). 4. As a result of analyzing the food pattern factors that have influence upon both major oral illnesses in the annually personal recognition, the person, who has the dangerous-type food pattern, had high risk level of the periodontal disease in the annually personal recognition. Conclusions : In the above results, as a result of surveying and analyzing importance of the food pattern in the incidence of both major oral illnesses, it is considered that there will be necessity of continuing to research into developing the nutritional policy and nutritional program in order to prevent oral illness in the future.

• 한국치위생학회지
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• 제9권1호
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• pp.101-111
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• 2009

Objectives : The purpose of this study was to evaluate the use of oral hygiene products in Korean adult population. And it was conducted to make a baseline in planning the operation of the oral health for the Korean adult. Methods : The National Survey data of Oral Health was used. The use of each oral hygiene product was analysed according to socioeconomic status (Age, Occupation, Residential district), smoking status and concern about one's oral health. The subject of this study were 14,368 of 2000 year, 3,526 of 2003 year and 2,439 of 2006 year. They were organized from 20s to 60s. Data was analysed into Pearson chi-square test and Fisher's exact test using SAS 8.01 program. Results : 1. The rate of using oral hygiene product was increased, but the rate of non-using people remains 65.8% on the 2006 data. 2. The non-using group of the oral hygiene products was the age of sixty, farmer, rancher, fisherman, military, ruralist, smoker and the people do not concern about their oral health(p<0.05). Conclusion : To improve the rate of using oral hygiene products, effective motivation method would be necessary. In addition, It is required to educate the group more individually. This is for them to have a interesting about oral hygiene products and learn how to choice appropriate product. In the future, study about the reason of non-using oral hygiene products, and more concrete method for improving their behavior would be needed.

• Asian Pacific Journal of Cancer Prevention
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• 제15권18호
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• pp.7625-7628
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• 2014

Breast cancer is the most common cancer among women worldwide. The primary aim of this work was to provide an in-depth evaluation of research publications in the field of diets and breast cancer. The impact of economic outcome on national academic productivity was also investigated. Data were retrieved using Pubmed for English-language publications. The search included all research for which articles included words relating to "diets and breast cancer". Population and national income data were obtained from publicly available databases. Impact factors for journals were obtained from Journal Citation Reports$^{(R)}$ (Thomson Scientific). There were 2,396 publications from 60 countries in 384 journals with an impact factor. Among them, 1,652 (68.94%) publications were Original articles. The United States had the highest quantity (51% of total) and highest of mean impact factor (8.852) for publication. Sweden had the highest productivity of publication when adjusted for number of population (6 publications per million population). Publications from the Asian nation increased from 5.3% in 2006 to 14.6% in 2012. The Original article type was also associated with geography (p<0.001; OR=2.183; 95%CI=1.526-3.123), Asian countries produced more proportion of Original articles (82%) than those of rest of the world (67.6%). Diets and breast cancer-associated research output continues to increase annually worldwide including publications from Asian countries. Although the United States produced the most publications, European nations per capita were higher in publication output.

• International Journal of Internet, Broadcasting and Communication
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• 제9권3호
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• pp.27-34
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• 2017

According to the material about the analysis of digital consumers' lifestyle in Korea published at the end of 2016, 100% had experienced a smartphone before, and 91% had joined in digital communications. Among digital contents, 85.5% had experienced music, and 78.6% had used TV contents before. Also, SNS utility rate equaled 83.3%, and the Facebook utility rate of those in the 10's and 20's exceeded 80%. It means that it is necessary to conduct research on SNS utility in broadcasts. Based on the results of operating KBS Facebook accounts, this paper aims to compare the reaches of entertainment, drama, and culture genres and analyze correlation between the reaches and 'like' friends on Facebook and also correlation between the reaches and ratings.To address the goal, among the Facebook mentions operated within a year, 2016, this researcher analyzed 2,526 mentions to publicize entertainment, drama, and culture genres and the results of 834 million reaches and also used the data of original broadcasts' ratings. To analyze the data, this study employed SPSS Ver. 18.0 to perform the independent sample t-test and Pearson's correlation analysis. The conference interval is set as 95%, and p<0.05 is regarded to be statistically significant. According to the results, more reaches are found in entertainment, drama, and culture genres in order, and it is found to have strongly positive correlation with the frequency of 'like's. The reaches and ratings have no correlation, and there is no statistical significance found in it. It is expected that the results of this study can be applied to publicize programs efficiently for broadcasting.

• 한국전기전자재료학회:학술대회논문집
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• 한국전기전자재료학회 2002년도 추계학술대회 논문집 Vol.15
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• pp.391-394
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• 2002

There has recently been much interest and research in developing digital x-ray systems based on using amorphous selenium(a-Se) photoconductors as the image receptor. The amorphous selenium layer that is currently being studied for use as an x-ray photoconductor is not pure a-Se but rather amorphous selenium alloyed with arsenic. We fabricated samples using the selenium and arsenic alloy with various concentrations of the arsenic. In this work, x-ray photoconductor using amorphous selenium alloyed with arsenic were fabricated with different concentrations of the arsenic (0.1 wt.%, 0.3wt.%, 0.5wt.%, 1wt.%, 1.5wt.%, 3wt.%, 5wt.%). The seven kind of samples was fabricated with a-Se alloyed with arsenic through vacuum thermal evaporation. We also investigate the arsenic concentration dependence on the device performance in radiation detector. The electric characteristics of radiation detector devices with changing additive ratio of the arsenic is performed by measuring the x-ray induced photocurrent and integrating it over time to find the total charge. The thickness of a-Se is $100{\mu}m$. Bias voltages $3V/{\mu}m$, $6V/{\mu}m$， $9V/{\mu}m$ are applied at the samples. As results, the net charge of a-Se 0.3% As sample is $526.0pC/mR/cm^2$ at $9V/{\mu}m$ bias. The net charge is decreased as with the increasing additive ratio of arsenic.