• Journal of Nutrition and Health
• /
• 제30권2호
• /
• pp.187-194
• /
• 1997

The effects of diet induced hyperlipidemia on kidney function were studied in Sprague-Dawley rats fed high fat diet containing 20% beef tallow and high cholesterol diet containing 5% cholesterol for 8, 12, 16 weeks, respectively. The concentrations of serum total lipid, total cholesterol and LDL-cholesterol were significantly high cholesterol diet groups during all experimental periods (p<0.05). HDL-cholesterol concentration was the lowest value in high cholesterol diet group of 16 weeks(p<0.05). Triglyceride concentration was not affected by experimental diets. Serum total protein, albumin and creatinine concentration tended to higher in high cholesterol diet groups than those in high fat diet groups. And serum urea-N concentration was higher in high fat diet group of 16 weeks than that in other diet groups. Urinary total protein and urea-N were higher in high cholesterol diet groups than those in other diet groups regardless of experimental period period. There was no significant difference in urinary creatinine concentratin among diet groups(p<0.05). GFR was lower in high cholesterol diet groups than that in high fat diet groups at 8, 16 weeks, respectively. Wet weight per body weight, total lipid, triglyceride, total cholesterol concentations of liver tissue were apparently high in high cholesterol diet groups(p<0.05). Kidney wet weight per body weight wer not affected by experimently diets, total lipid concentration of kidney tissue was significantly high in high fat diet groups of 12 weeks(p<0.05), kidney tissue triglyceride concentrations of high cholesterol diet groups of 12, 16 weeks apparently low, and total cholesterol concentration of kidney tissue was higher in experimental diet groups than that of control groups at 12, 16 weeks(p<0.05). Fecal excretion, total lipid, triglyceride and cholesterol concentrations of feces were markedly high in all high cholesterol diet groups except high fat diet group of 16 weeks. The results of light microscopic examination indicated that glomerulosclerosis was not obsrved in rats fed experimental diets.

• Asian Pacific Journal of Cancer Prevention
• /
• 제16권16호
• /
• pp.6845-6849
• /
• 2015

Background: Roles of tobacco and alcohol use in etiology of cancer are well established. Alterationin in P53 have essential roles neoplastic change by preventing genome mutation; the aim of this study was to assess the association between P53 mutation and tobacco and alcohol consumption, as well as to assess the epidemiology of tobacco and alcohol use as risk factors for cancer in the adult population of northern Saudi civilians. Materials and Methods: A cross-sectional survey from October 2014 to January 2015, covering 3,398 adults, was performed. P53 mutation molecular detection was performed for 100 tobacco and alcohol users, usingDNA extracted from buccal cells. Results: Of the 3,398 participants 3,253/3398(95.7%) responded, with a male female ratio of 1.10: 1.00. Out of these, 24.8% had smoked tobacco in their lifetime and 2.7% were consumers of alcoholic beverages. None was identified with any P53 mutation. Conclusions: The prevalence of tobacco smoking among the northern Saudi civilians was relatively high. Females' attitudes in tobacco and alcohol related issues were found to be affected by social stigma. Tobacco and alcohol use has no link to P53 gene mutations.

• The Plant Pathology Journal
• /
• 제16권5호
• /
• pp.252-256
• /
• 2000

The 16S-23S rRNA intergenic spacer regions (ISRs) were sequenced and analyzed to design specific primer for identification of Pectobacterium chrysanthemi. Two types ISRs, large and small ISRs, were identified from three strains (ATCC 11663, KACC 10163 and KACC 10165) of P. chrysanthemi and Pectobacterium carotovorum subsp. carotovorum ATCC 15713.Large ISRs contained transfer RNA-Ile(tRNA$^{Ile}$)and tRNA$^{Ala}$, and small ISRs contained tRNA$^{Glu}$. Size of the small ISRs of P. chrysanthemi ranged on 354-356 bp, while it was 451 bp in small ISR of P. carotovorum subsp. carotovorum ATCC 15713. From hypervariable region of small ISRs, species-specific primer for P. chrysanthemi with 20 bp length (CHPG) was designed from hypervariable region of small ISRs, which was used as forward promer to detect P. chrysanthemi strains with R23-1R produced PCR product of about 260bp size (CHSF) only from P. chrysanthemi strains, not from other Pectobacterium spp. and Erwinia spp. Direct PCR from bacterial cell without extracting DNA successfully amplified a specific fragment, CHSF, from P. chrysanthemi ATCC 11663. The limit of PCR detection was 1${\pm}10^2$ cfu/ml.

• 한국양식학회지
• /
• 제11권1호
• /
• pp.1-10
• /
• 1998

돌돔 사료의 적정 단백질 및 지질 함량을 구하기 위해 북양어분과 카제인을 단백원으로 하여 사료의 단백질 함량이 30, 40, 50 및 60%가 되도록 하고, 각 단백질 함량에 지질 함량이 8 및 16%가 되는 8종의 실험사료로 평균체중 7 g의 돌돔을 대상으로 8주간 사육 실험하였다. 사료의 단백질 함량에 따른 증체율 및 사료효율은 사료지질 8% 수준에서 사료단백질이 40% 이상인 사료구에서는 사료단백질 함량이 증가함에 따라 유의차 있게 증가하였으며(P<0.05), 사료지질 16% 수준에서는 사료단백질이 증가함에 따라 증가하다가, 사료단백질 50% 이상에서는 유의적인 차이가 없었다(P>0.05). 사료지질 16% 수준에서 broken line model을 이용하여 증체율을 지표로 하여 돌돔의 적정 단백질 요구량을 구한 결과 46%로 추정되었다. 사료의 지질 함량에 따른 증체율은 사료단백질 40 및 50% 수준에서 고지질 사료구(16%)가 유의하게 높았으며, 사료효율은 모든 사료단백질 수준에서 고지질 사료구(16%)가 유의하게 높았다(P<0.05). 단백질 효율 및 단백질 축적율은 모든 사료단백질 수준에서 고지질 사료구가 높았다. 따라서 성장과 사료효율을 기준으로 볼 때 돌돔 사료의 적정 지질 함량은 16% 전후로 추정되었다. 전어체 및 등근육의 성분에서 단백질은 사료의 단백질 함량이 증가함에 따라 증가하다가 사료 단백질 40% 이상에서는 유의차가 없었으며, 지질은 모든 사료단백질 수준에서 고지질 사료구가 높았다. 간 및 내장의 성분에서 단백질은 사료의 단백질 함량에 따라 뚜렷한 경향이 없었지만, 지질은 모든 사료단백질 수준에서 고지질 사료구(16%)가 높았다.

• The Journal of Korean Physical Therapy
• /
• 제18권1호
• /
• pp.95-106
• /
• 2006

Purpose: The aim of this study was to investigate the kinematics of young adults during ramp climbing at different inclinations. Methods: Twenty-three subjects ascended and descended four steps at four different inclinations(level, $8^{\circ},\;16^{\circ},\;24^{\circ}$). Temporal-spatial parameters were measured by GaitRite system(standard mat). Groups difference was analysed with on-way ANOVA and Student-Newman-Keuls test. Results: The different kinematics of ramp ascent and descent were analysed and compared to level walking patterns. In ascending ramps, step length and stride length decreased with $24^{\circ}$ inclination (p<.000). Stance duration increased with $24^{\circ}$, but swing duration decreased with $24^{\circ}$ inclination (p<.000). Step time and velocity decreased with $16^{\circ}C,\;24^{\circ}$ inclination (p<.000). Cadence decreased with all inclination($8^{\circ},\;16^{\circ},\;24^{\circ}$)(p<.000). In descending ramps, step length and stride length, velocity decreased with all inclination($8^{\circ},\;16^{\circ},\;-24^{\circ}$)(p<.000). Stance duration increased with all inclination($8^{\circ},\;-16^{\circ},\;-24^{\circ}$) and swing duration decreased with all inclination($-8^{\circ},\;-16^{\circ},\;-24^{\circ}$)(p<.000). But Step time was not differentiated with different inclinations. Cadence decreased with only. $8^{\circ}$ inclination(p<.05). Conclusion: These results suggest that there is a certain inclination angle or angular range where subjects do switch between level walking and ramp walking gait pattern. This shows their motor control strategy between level and ramp walking. Further studies are necessary to confirm and detect the ascent and descent ramp gait patterns.

• Asian Pacific Journal of Cancer Prevention
• /
• 제16권16호
• /
• pp.7195-7200
• /
• 2015

Background: Since seventeen employees of an offset printing company in Osaka, Japan developed cholangiocarcinoma it has become recognized as an occupational cancer. This study investigated the differences of clinical features between occupational cholangiocarcinoma and sporadic young-onset cholangiocarcinoma. Materials and Methods: Thirty-four young adults (<50 years old) with sporadic cholangiocarcinoma were extracted from the Rosai Hospital Group database (sporadic group) and their clinical features were compared with those of 17 patients with occupational cholangiocarcinoma (occupational group). Results: The 34 patients in the sporadic group were treated for cholangiocarcinoma at 16 different Rosai hospitals. There were significant differences of age (p<0.01), gender (p<0.01), abnormal laboratory tests (p<0.01), and tumor location (p<0.01) between the two groups. The percentage of patients with abnormal laboratory tests was significantly higher in the occupational group than in the sporadic group (p<0.001). Regional dilation of bile ducts, which is a characteristic of occupational cholangiocarcinoma, was not observed in the sporadic group. Conclusions: No cluster of cholangiocarcinoma cases was identified in the Rosai Hospital database. There were differences of clinical features between occupational and sporadic cholangiocarcinoma, which might be helpful for diagnosing occupational cholangiocarcinoma in the future.

• Asian Pacific Journal of Cancer Prevention
• /
• 제15권23호
• /
• pp.10289-10292
• /
• 2015

Background: The involvement of HPV in oral and oropharyngeal carcinogenesis was first proposed in 2004, based on epithelial HPV tropism and detection of HPV genotypes in oral squamous cell carcinoma samples. While 60-70% of oropharynx tumors may be HPV-positive, only 10 to 19% of tumors of the oral cavity, larynx and hypopharynx appear to have HPV infection. The aim of the study was to evaluate HPV infection associated with oropharyngeal cancer. Materials and Methods: Seventy-eight cases were selected for p16 immunoexpression reactions, and demographic data were collected for comparisons. Results: Most patients were over 60 years old, and 64.1% were smokers. Immunohistochemistry results showed that 86.3% of cases stained positive for p16 protein. Conclusion: The oropharyngeal cancer profile at Erasto Gaertner Hospital presented a high index of smokers over 60 years as well a high number of p16+ tumors, for what we can not determinate the main etiologic factor, but can be aware of the number of patients that presented HPV infection. Since prevention is still the best way to deal with cancer disease, it is important to analyze the interaction of these two etiologic factors and how to detect lesions at an early stage.

• Journal of Interdisciplinary Genomics
• /
• 제1권1호
• /
• pp.10-13
• /
• 2019

A 10-year and 5 month-old girl with developmental delay, intellectual disability, attention deficit hyperactivity disorder, poor weight gain, and microcephaly was transferred to our pediatric clinic for genetic evaluation. Her height was within the 5-10th percentile, and her weight was under the 3rd percentile. On the social maturity scale, her developmental status was scored as 3 years 9 months for social age, and the social quotient was 35.98. A chromosomal microarray analysis was performed and the microduplication at chromosome 16p was observed: arr[GRCh37] 16p11.2 (29580020_30190029)${\times}3$. Currently, the patient is diagnosed with Grade 2 intellectual disability and is attending a computerized cognitive rehabilitation class twice weekly. In addition, nutritional support and growth follow up are also ensured in the Pediatric Gastrointestinal and Endocrinology clinic.

• Journal of the Korean Wood Science and Technology
• /
• 제38권6호
• /
• pp.541-546
• /
• 2010

본 연구의 목적은 3시간 오존산화 처리식용유와 pMDI를 혼합하여 고온과 중온에서의 접착제를 제조하였고, 이를 상태 인장전단 접착강도 시험을 통하여 식용유의 목재 접착제 응용 가능성을 확인하였다. 고온조건에서 3시간 오존산화 식용유의 상태 접착강도 시험 결과, 오존산화 식용유 : pMDI 비율이 1 : 0.5일 때 4.74 kgf/$cm^2$, 1 : 0.75일 때 7.14 kgf/$cm^2$, 1 : 1일 때 9.29 kgf/$cm^2$, 1 : 2일 때 16.53 kgf/$cm^2$, 1 : 3일 때 17.42 kgf/$cm^2$, 1 : 4일 때 16.75 kgf/$cm^2$를 나타냈다. 따라서 3시간 오존산화 식용유 : pMDI 비율이 1 : 2∼1 : 3일 때 가장 높은 접착력을 보임을 알 수 있어, 두 물질의 적정 당량비가 이 비율에서 형성됨을 알 수 있었다. 중온조건에서 3시간 처리한 식용유의 상태 접착강도 시험 결과, 식용유 : pMDI 비율이 1 : 0.5일 때 3.16 kgf/$cm^2$, 1 : 0.75일 때 6.13 kgf/$cm^2$, 1 : 1일 때 8.18 kgf/$cm^2$ 1 : 2일 때 11.82 kgf/$cm^2$이 나타났다. 즉, 고온, 중온에서의 오존산화 처리한 식용유:pMDI 혼합비율이 1 : 2~1 : 3 부근에서 높은 접착력을 보였고, 목재접착제로서 고온에서 접착 할 경우 합판에서의 이용 가능성이 높을 것으로 판단된다.

• Asian Pacific Journal of Cancer Prevention
• /
• 제13권12호
• /
• pp.6083-6086
• /
• 2012

Background: The aim of this study was to screen for human papillomavirus (HPV) infections in head and neck squamous cell carcinomas (HNSCCs) using P16 immunostaining. Materials and Methods: A retrospective study was performed on 150 samples from patients diagnosed with HNSCCs. HPV status was determined using $p16^{INK4A}$. Results: 31 of the 150 (20.7%) HNSCCs were HPV positive. Conclusions: A large proportion of HNSCCs in Sudan are associated with HPV infection. The fact that the prevalence of HPV is high among Sudanese patients with head and neck cancers (HNC) has obvious implications for vaccine therapy.