• Journal of Veterinary Clinics
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• v.28 no.5
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• pp.538-541
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• 2011

An 8-year-old spayed Yorkshire Terrier Dog was presented to the Veterinary Teaching Hospital of Kyungpook National University because of the recurrent superficial pyoderma. At the presentation, pustules and papules were present throughout the body. Numerous rods with a few cocci were observed on impression smears and they were confirmed to be Proteus mirabilis and Staphylococcus pseudointermedius consecutively. The patient was treated with systemic enrofloxacin and amoxicillin-clavulanic acid based on the results of antimicrobial sensitivity tests with once a week basis 4% chlorhexidine shampoo. An excellent clinical response was achieved in 2 weeks of therapy and the lesions were fully resolved in 6 weeks. The possibility of P. mirabilis infection should not be overlooked by clinicians in dogs with recurrent superficial pyoderma although it's been considered to be rare.

• Proceedings of the Korean Society for Applied Microbiology Conference
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• 1986.12a
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• pp.526.2-526
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• 1986

P. mirabilis 로부터 정제한 cysteinylglycine 분해효소의 성질 및 세포내 국재성을 검토하였다. 본 효소의 일반적 성질은 pH가 7.3 온도 37$^{\circ}C$에서 최대 활성을 냐타내었으며 pH 8.0에서 안정하였고, 열안정성은 $50^{\circ}C$, 30분처리에 30%의 활성 손실을 보였다. 또한 $Mn^{+2}$이온과 $Mg^{+2}$ 이온에 의해 활성이 촉진되었으며 본 효소를 반응전 30분 Preincubation 하므로서 최대 활성을 보였다. 본 효소는 glutathione 일단계 분해효소인 ${\gamma}$-glutamyl transpeptidase와 마찬가지로 세포내의 periplasmic space에 존재하였다.

• Journal of the Korean Chemical Society
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• v.32 no.5
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• pp.422-427
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• 1988

The bio-electrode for L-asparagine, excellent in the reproducibility of responsibility, has been constructed by immobilizing the bacterium Proteus mirabilis on an ammonia gas sensor. This electrode was investigated for the effects of pH, temperature, buffer solution, bacterial amounts and interferences, and stability with the lapse of time. The response of the bacterial electrode was linear in the range of $9.0{\times}10^{-5}$ ∼ $1.0{\times}10^{-2}M$ L-asparagine with a slope of 58.9mV/decade in pH 7.8, 0.05M phosphate buffer solution at $30^{\circ}C$. The bacterial amounts used for this electrode was 3 mg and response time was 7∼9 min. Therefore, this assembly can be used for the determination of L-asparagine.

• Archives of Pharmacal Research
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• v.9 no.3
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• pp.163-167
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• 1986

Twenty six urea analogues, most of which have already been approved for human use, were tested for their antiurease activity in vitro. Cell-free extracts obtained from a clinical isolate of Proteus mirabilis was used as the source of enzyme. Acetohydroxamic acid which is a proven potent urease inhibitor but not approved for human use was again shown to be the most active compound among the tested. Phenacemide, cycloserine, and deferoxamine were demonstrated to be moderate inhibitors. Oxtetracycline, trimethoprim, and cefamandole revealed a demonstrable antiruease activity, but only at very high concentrations. The antiurease activity of cycloserine, trimethoprim, and cefamandole was pH dependent-only active at acidic pH. The inhibitory activity of acetohydroxamic acid however was independent of change in pH. The inhibitory activity of acetohydroxamic acid however was independent of change in pH. Hydrogen ion concentration plays an important role in urease activity and acidification (pH 5. 5) alone eliminates approximately 65% of the enzymic activity. Adjustment of pH therefore appears to be an important adjunct in reducing unrease activity and should always be studied to maximize the effcacy of antiurease compounds under investigation.

• Korean Journal of Veterinary Service
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• v.32 no.3
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• pp.215-218
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• 2009

The dog breeding kennel bacterial infection are very significant in perinatal mortality. In many case, Staphylococcus aureus, Streptococcus, and Escherichia coli were infected in intra-uterine or by the genital tract to the puppies, and they are cause of septicemic death of the puppies and clinical mastitis of bitch, leading to septicemic death of newborn puppies. Severe mastitis due to bacterial infection was diagnosed in a 2 year-old female Brittany Spaniel which loss 6 puppies with odor small rice sharp white diarrhea of nine puppies. Bright curd milk and intestinal sample were inoculated on MacConkey agar, blood agar and brain heart infusion agar, and incubated at $37^{\circ}C$ for 24-48 hrs. Gram negative colonies isolated from these sample which were characterized as Glu, Ure, $H_2S$, Orn, Cit, and Cl, and were identified by Microscan Walk-Aways Baxter, American Type Culture Collection, USA) as Proteus mirabils. The isolate was more sensitive to ampicillin, gentamicin, cefoxitin, cefuroxime, and cefazidime. In this results, we confirmed that cause bacteria of septicemic death in puppies was P. mirabilis.

• Tuberculosis and Respiratory Diseases
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• v.78 no.4
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• pp.371-374
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• 2015

Pulmonary pneumatoceles are air-filled thin-walled spaces within the lung and are rare in adult cases of pneumonia. We report the case of a 74-year-old male who was admitted with a cough and sputum production. He had been treated with oral dexamethasone since a brain tumorectomy 6 months prior. Contrast-enhanced computed tomography (CT) of the chest revealed a large pneumatocele in the right middle lobe and peripheral pneumonic consolidation. Bronchoalveolar lavage was performed; cultures identified extended-spectrum ${\beta}$-lactamase (ESBL) producing Proteus mirabilis. A 4-week course of intravenous ertapenem was administered, and the pneumatocele with pneumonia resolved on follow-up chest CT. To the best of our knowledge, this is the first reported case of pulmonary pneumatocele caused by ESBL-producing P. mirabilis associated with pneumonia.

• Journal of the Korean Chemical Society
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• v.35 no.1
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• pp.38-45
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• 1991

The cytidine bio-sensors have been constructed by immobilizing the bacterium Proteus mirabilis and organelle on an ammonia gas sensor. The bacterial sensor was investigated for the effects of pH, temperature, buffer solution, bacterial amounts, interferences and lifetime. The bacterial sensor had linearity in the range of 5.0 ${\times}$ 10$^{-4}$M ∼ 1.0 ${times}$ 10$^{-2}$M cytidine with a slope of 56 mV/decade at pH 7.8, 30$^{\circ}C$ and 3 mg in 1.0 M phosphate buffer solution. This bacterial sensor was compared with it's organelle sensor.

• Food Science and Preservation
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• v.15 no.2
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• pp.300-305
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• 2008

We investigated an antibacterial substance produced by Bacillus subtilis HS 25. Antibacterial activity was relatively heat-stable, with no effect caused by heating at $100^{\circ}C$ for 10 min, but a gradual decrease in activity after 15 min at $100^{\circ}C$. The antibacterial substance was more stable at pH 7.42-12 than at pH 4.5-5.0. There was strong antibacterial activity against E. coli and P. mirabilis at pH 12. The minimum inhibition concentration (MIC) of the substance was 5 mg/mL for E. coli 7.5 mg/mL for P. mirabilis and S. aureus and 15 mg/mL for S. enteritidis, K. pneunoniae, and V. parahaemolyticus. When the substance was added to cultures of E. coli, S. enteritidis, and P. mirabilis, the bacterial surfaces became irregular and deeply changed. The substance produced from B. subtilis HS 25 was not degraded by Papain but was degraded by a protease from Aspergillus orzae, pancreatin, and pepsin.

• Food Science and Preservation
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• v.15 no.5
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• pp.774-781
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• 2008

was performed and Staphylococcus sciuri, Bacillus cereus, Escherichia coli, Proteus mirabilis, and Enterococcus faecalis were identified. No Salmonella strain, a typical contaminant of eggs, was found. The radiation sensitivities of isolated bacteria and Salmonella typhimurium, in an inoculated model system, were expressed in $D_{10}$ values. The ranges of $D_{10}$ values shown by S. typhimurium, S. sciuri, B. cereus, E. coli, P. mirabilis, and E. faecalis were 0.365-0.399 kGy, 0.418-0.471 kGy, 1.075-1.119 kGy, 0.280-0.304 kGy, 1.132-1.330 kGy, and 0.993-1.290 kGy, respectively. The growth of all six test bacteria in eggs (inoculated at $10^6-10^7\;CFU/g$) during 3 days of post-irradiation storage at ambient conditions ($25^{\circ}C$) was recorded. S. typhimurium was eliminated by irradiation at 3 kGy, and E. coli and S. sciuri were eliminated by irradiation at 5 kGy. The viable cell counts of B. cereus, P. mirabilis, and E. faecalis in eggs showed 4-6 log reductions after irradiation with 5 kGy.

• Korean Journal of Microbiology
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• v.29 no.3
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• pp.195-198
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• 1991

Isolation and identification of mesophilic and psychrotrophic bacteria distributed in Korean refrigerated beef were attempted. Total isolated colonies were 192, and identified as 5 genera and 10 species. Among them, mesophilic bacteria were Enterobacter aerogenes, E. agglomerans, Serratia liquefaciens, Proteus mirabilis, and "psychrotrophic" bacteria were Pseudomons fluorescens, P. putida, P. pickettii, P. mendocina, P. stutzeri, Alcaligenes faecalis. Dominant species was Serratia liquefaciens as mesophiles, and Pseudomonas putida as psychrotroph.chrotroph.