• Title/Summary/Keyword: P. florida

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Comparisons of Soluble Protein Bands for Pleurotus Species and Interspecific Crosses of Pleurotus Species (느타리버섯속균과 교잡종에 대한 단백질 밴드에 의한 분류)

  • Kim, Myong-Jo;Shim, Jae-Ouk;Lee, Youn-Su
    • The Korean Journal of Mycology
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    • v.25 no.1 s.80
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    • pp.20-25
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    • 1997
  • In comparison of three Pleurotus species and their selfed and crossed isolates using SDS-polyacrylamide gel electrophoresis of total soluble proteins, Pleurotus ostreatus 201 showed low similarity to selfed or P. ostreatus 201 crossed ones. Pleurotus ostreatus 2042 showed low similarity to selfed or P. ostreatus 2042 crossed ones. However, P. ostreatus $2042{\times}P$. ostreatus 202, P. ostreatus $2042{\times}P$. sajor-caju, and P. ostreatus $2042{\times}P$. ostreatus 900 showed high similarity. Pleurotus ostreatus 202 showed low similarity to selfed or crossed ones. Pleurotus sajorcaju showed low similarity to selfed or crossed ones. Pleurotus ostreatus 900 showed low similarity to selfed or crossed ones. However, selfed P. ostreatus and P. $ostreatus{\times}P$. florida showed high similarity. Pleurotus florida and selfed P. florida showed high similarity, too.

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Virus-like Particles from Abnormal Growing Oyster Mushrooms, Pleurotus florida and P. ostreatus (균사생장(菌絲生長)이 부진(不進)한 사철느타리 및 느타리버섯으로부터 Virus입자(粒子)의 분리(分離))

  • Go, Seung-Joo;Cha, Dong-Yeul;Shin, Gwan-Chull
    • The Korean Journal of Mycology
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    • v.20 no.2
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    • pp.149-153
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    • 1992
  • This study aimed to investigate possible cause of slow and abnormal growth of oyster mushrooms, Pleurotus florida and P. ostreatus collected from bad crop farms. Spherical virus particles of 30 nm in diameter from P. florida, 23 nm particles from P. ostreatus, and both 23 and 30 nm particles were also found from interspecies mated culture between P. florida and P. osreatus. The virus particles might be associated with the bad crop of Pleurotus species.

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Isolation, Regeneration and PEG-Induced Fusion of Protoplasts of Pleurotus pul-monarius and Pleurotus florida

  • Eyini, M.;Rajkumar, K.;Balaji, P.
    • Mycobiology
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    • v.34 no.2
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    • pp.73-78
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    • 2006
  • Inter-specific hybridization between Pleurotus pulmonarius and P. florida was attempted through PEG-induced protoplast fusion to select a fusant. The protocol for protoplast release, regeneration and fusion in these two Pleurotus species was standardized using the variables controlling the process. The mixture of mycolytic enzymes, i.e. commercial cellulase, crude chitinase and pectinase, KCl (0.6 M) as osmotic stabilizer, pH 6 of the phosphate buffer and an incubation time of 3 hours resulted in the maximum release of protoplasts from 3-day-old mycelia of P. florida ($5.3{\sim}5.75{\times}10^{7}$ protoplasts/g) and P. pulmonarius ($5.6{\sim}6{\times}10^{7}$ protoplasts/g). The isolated protoplasts of P. florida regenerated mycelium with 3.3% regeneration efficiency while P. pulmonarius showed 4.1% efficiency of regeneration. Polyethyleneglycol (PEG)-induced fusion of protoplasts of these two species resulted in 0.28% fusion frequency. The fusant produced fruiting bodies on paddy straw but required a lower temperature of crop running ($24{\pm}2^{\circ}C$) than its parents which could fruit at $28{\pm}2^{\circ}C$. The stable fusant strain was selected by testing for the selected biochemical markers i.e. Carbendazim tolerance and utilization of the lignin degradation product, vanillin.

The Effects of Hydrogen Peroxide Pretreatment on Rice Straw Fermentation for Feed (볏짚 발효사료 제조시 과산화수소 전처리 효과)

  • Choi, Yoon-Hee;Lee, Sang-Bok;Kim, Myeong-Sook;Hong, Jai-Sik
    • Applied Biological Chemistry
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    • v.37 no.5
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    • pp.326-333
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    • 1994
  • Pleurotus florida with high cellulase activity as well as lignin degradability was selected out among strains for fermentation of the rice straw to improve the nutritive value. When the rice straw was fermented by P. florida, the contents of hemicellulose, cellulose and lignin were decreased to 22.5%, 11.4% and 28.1%, respectively, whereas the contents of rice straw fermented after pretreatment with $H_2O_2$ or alkaline hydrogen peroxide were decreased much in the lower concentration. The content of T-N (total-nitrogen) and crude fat was increased with the longer fermentation period. The amino acid content of rice straw fermented by P. florida in 30 days was increased to 28.9% and 35.1% as the rice straw was fermented after pretreatment without and with 4% $H_2O_2$, respectively. The crystalline intensity of rice straw was decreased by pretreatment with 4% $H_2O_2$ and fermentation by P. florida. However, the crystall intensity was increased by treatment with alkaline hydrogen peroxide and the more when the straw was washed after the treatment. When the rice straw was fermented by P. florida for 30 days, the in vitro organic matter digestibility was increased up to 6% of $H_2O_2$ pretreatment.

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Health Disparities between Black Hispanic and Black Non-Hispanic Cervical Cancer Cases in the USA

  • Khan, Hafiz Mohammad Rafiqullah;Gabbidon, Kemesha;Abdool-Ghany, Faheema;Saxena, Anshul;Gomez, Esneider;Stewart, Tiffanie Shauna-Jeanne
    • Asian Pacific Journal of Cancer Prevention
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    • v.15 no.22
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    • pp.9719-9723
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    • 2014
  • Background: Globally, cervical cancer is a major public health concern. Cervical cancer is the second most common cancer among women, resulting in approximately 500,000 cases per year. The purpose of this study is to compare disease characteristics between Black Hispanic (BH) and Black non-Hispanic (BNH) women in the US. Materials and Methods: We used stratified random sampling to select cervical cancer patient records from the SEER database (1973-2009). We used Chi-square and independent samples t-test to examine differences in proportions and means. Results: The sample included 2,000 cervical cancer cases of Black non-Hispanic and 91 Black Hispanic women. There were statistically significant differences between black Hispanic and black non- Hispanics in mean age at diagnosis (p<0.001), mean survival time (p<0.001), marital status (p<0.001), primary site of cancer (p<0.001); lymph node involvement (p<0.001); grading and differentiation (p<0.0001); and tumor behavior (p<0.001). Black women were more likely to develop cervical cancer and to have the highest mortality rates from the disease. Conclusions: Findings from this study show clear racial and ethnic disparities in cervical cancer incidence and prognosis that should be addressed.

Progress in Novel Oxides for Gate Dielectrics and Surface Passivation of GaN/AlGaN Heterostructure Field Effect Transistors

  • Abernathy, C.R.;Gila, B.P.;Onstine, A.H.;Pearton, S.J.;Kim, Ji-Hyun;Luo, B.;Mehandru, R.;Ren, F.;Gillespie, J.K.;Fitch, R.C.;Seweel, J.;Dettmer, R.;Via, G.D.;Crespo, A.;Jenkins, T.J.;Irokawa, Y.
    • JSTS:Journal of Semiconductor Technology and Science
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    • v.3 no.1
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    • pp.13-20
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    • 2003
  • Both MgO and $Sc_2O_3$ are shown to provide low interface state densities (in the $10^{11}{\;}eV^{-1}{\;}cm{\;}^{-2}$ range)on n-and p-GaN, making them useful for gate dielectrics for metal-oxide semiconductor(MOS) devices and also as surface passivation layers to mitigate current collapse in GaN/AlGaN high electron mobility transistors(HEMTs).Clear evidence of inversion has been demonstrated in gate-controlled MOS p-GaN diodes using both types of oxide. Charge pumping measurements on diodes undergoing a high temperature implant activation anneal show a total surface state density of $~3{\;}{\times}{\;}10^{12}{\;}cm^{-2}$. On HEMT structures, both oxides provide effective passivation of surface states and these devices show improved output power. The MgO/GaN structures are also found to be quite radiation-resistant, making them attractive for satellite and terrestrial communication systems requiring a high tolerance to high energy(40MeV) protons.

Clinical Comparison Of Manual Probe With Florida Probe In Adult Periodontitis (성인형 치주염 환자에 있어 manual probe의 Florida probe의 임상적 비교)

  • Yu, Hyang-Mi;Chung, Chin-Hyung
    • Journal of Periodontal and Implant Science
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    • v.26 no.1
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    • pp.244-254
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    • 1996
  • The periodontal probe is a commonly used instrument to assess periodontal conditions. And so, there has been many studies to develop the accuracy and reproducibility of the periodontal probe. The purpose of this study was to compare two different periodontal probes for measurement reliability and time required to use in subjects with moderate periodontitis. It was done after evaluating reproducibility of probing depth by stent guiding for a Manual probe and a Florida probe in subjects with healthy periodontal condition. The results were as follows 1. In experiment to evaluate the reproducibility of probing depth by stent guiding for a Manual probe and Florida probe in subjects with healthy periodontal condition, there was no major significant difference between intraprobe and interprobe relationships. 2. There were reduced probing measuremint error by using the Florida probe for posterior teeth and by using the Manual probe for anterior teeth of subjects with moderate periodontitis. 3. At proximal area, there was higher measurement error by using the Manual probe than the Florida probe. 4. The mean of pocket depth measurement using Manual probe was signifi cantly higher than that using Florida probe(p<0.05). With increasing pocket depth, interprobe difference increased and reproducibility reduced. 5. There was no significant difference in time required to use between Manual probe and Florida probe(p<0.05). 6. There was slight probing measurement difference between Manual probe and Florida probe at different site, but both probes have similar degrees of reproducibility and similar time required to probe.

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Influence of lateralized versus medialized reverse shoulder arthroplasty design on external and internal rotation: a systematic review and meta-analysis

  • Kevin A. Hao;Robert J. Cueto;Christel Gharby;David Freeman;Joseph J. King;Thomas W. Wright;Diana Almader-Douglas;Bradley S. Schoch;Jean-David Werthel
    • Clinics in Shoulder and Elbow
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    • v.27 no.1
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    • pp.59-71
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    • 2024
  • Background: Restoration of external (ER) and internal rotation (IR) after Grammont-style reverse shoulder arthroplasty (RSA) is often unreliable. The purpose of this systematic review was to evaluate the influence of RSA medio-lateral offset and subscapularis repair on axial rotation after RSA. Methods: We conducted a systematic review of studies evaluating axial rotation (ER, IR, or both) after RSA with a defined implant design. Medio-lateral implant classification was adopted from Werthel et al. Meta-analysis was conducted using a random-effects model. Results: Thirty-two studies reporting 2,233 RSAs were included (mean patient age, 72.5 years; follow-up, 43 months; 64% female). The subscapularis was repaired in 91% (n=2,032) of shoulders and did not differ based on global implant lateralization (91% for both, P=0.602). On meta-analysis, globally lateralized implants achieved greater postoperative ER (40° [36°-44°] vs. 27° [22°-32°], P<0.001) and postoperative improvement in ER (20° [15°-26°] vs. 10° [5°-15°], P<0.001). Lateralized implants with subscapularis repair or medialized implants without subscapularis repair had significantly greater postoperative ER and postoperative improvement in ER compared to globally medialized implants with subscapularis repair (P<0.001 for both). Mean postoperative IR was reported in 56% (n=18) of studies and achieved the minimum necessary IR in 51% of lateralized (n=325, 5 cohorts) versus 36% (n=177, 5 cohorts) of medialized implants. Conclusions: Lateralized RSA produces superior axial rotation compared to medialized RSA. Lateralized RSA with subscapularis repair and medialized RSA without subscapularis repair provide greater axial rotation compared to medialized RSA with subscapularis repair. Level of evidence: 2A.

Effects of Geography, Weather Variability, and Climate Change on Potato Model Uncertainty

  • Fleisher, D.H.;Condori, B.;Quiroz, R.;Alva, A.;Asseng, S.;Barreda, C.;Bindi, M.;Boote, K.J.;Ferrise, R.;Franke, A.C.;Govindakrishnan, P.M.;Harahagazwe, D.;Hoogenboom, G.;Naresh Kumar, S.;Merante, P.;Nendel, C.;Olesen, J.E.;Parker, P.S.;Raes, D.;Raymundo, R.;Ruane, A.C.;Stockle, C.;Supit, I.;Vanuytrecht, E.;Wolf, J.;Woli, P.
    • Proceedings of The Korean Society of Agricultural and Forest Meteorology Conference
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    • 2016.09a
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    • pp.41-43
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    • 2016
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Transformation of Pleurotus florida with Neurospora pyr 4 Gene (Neurospora pyr 4 유전자를 이용한 사철 느타리버섯의 형질전환)

  • Byun, Myung-Ok;Yoo, Young-Bok;You, Chang-Hyun;Cha, Dong-Yeul;Cho, Moo-Je
    • The Korean Journal of Mycology
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    • v.17 no.4
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    • pp.209-213
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    • 1989
  • Transformation of an auxotrophic requirement for uracil in Pleurotus florida P101 has been achieved using chimeric vector containing Aspergillus nidulans ans 1, and Neurospora crassa pyr 4 DNA. Protoplasts of $Ura^-$strains of P. florida were incubated with plasmid pDJB3 containing the cloned pyr 4 gene in the presence of polyethylene glycol and $CaCl_2$. Transformants could grow on MMM showing mitotical stability. Southern hybridization analysis of DNA isolated from transformants showed that the Neurospora pyr 4 gene and vector sequence might be integrated into the P. florida chromosomes. As the transformants were monokaryon, each transformant was mated with the other monokaryon. Fruitbody shape of untransformant was eroded type but those of transformants were eroded type, funnel type, plane type and ungrowing cap type.

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