• 전기학회논문지
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• 제61권2호
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• pp.347-353
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• 2012

In this paper, authors will develop the quick and precise remote controller of automatic fire detection equipment (P-type one-class receiver) based on information communication technology (IT). The remote controller detects the fire and disaster in the building automatically and quickly and then activates the facilities to extinguish the fire and disaster, monitoring such situation in a real time through wire-wireless communication network. The proposed remote controller is applied a programmable logic device (PLD) micom. of one-chip type which is small size and lightweight and also has highly sensitive-precise reliabilities. The one-chip type PLD micom. analyzes digital signals from sensors, then activates fire extinguishing facilities for alarm and rapid suppression in a case of fire and disaster. The detected data is also transferred to a remote situation room through wire-wireless network of RS232c and bluetooth communication, and then the situation room sends an emergency alarm signal. The automatic fire detection equipment (AFDE) based on IT will minimize the life and wealth loss while prevents fire and disaster.

• Journal of Microbiology and Biotechnology
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• 제20권2호
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• pp.301-310
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• 2010

Bacillus subtilis strains produce a broad spectrum of bioactive peptides. The lipopeptide surfactin belongs to one well-known class, which includes amphiphilic membrane-active biosurfactants and peptide antibiotics. Both the srfA promoter and the ComP-ComA signal transduction system are an important part of the factor that results in the production of surfactin. Bs-M49, obtained by means of low-energy ion implantation in wild-type Bs-916, produced significantly lower levels of surfactin, and had no obvious effects against R. solani. Occasionally, we found strain Bs-M49 decreased spore formation and the development of competence. Blast comparison of the sequences from Bs-916 and M49 indicate that there is no difference in the srfA operon promoter PsrfA, but there are differences in the coding sequence of the comA gene. These differences result in three missense mutations within the M49 ComA protein. RT-PCR analyses results showed that the expression levels of selected genes involved in competence and sporulation in both the wild-type Bs-916 and mutant M49 strains were significantly different. When we integrated the comA ORF into the chromosome of M49 at the amyE locus, M49 restored hemolytic activity and antifungal activity. Then, HPLC analyses results also showed the comA-complemented strain had a similar ability to produce surf actin with wild-type strain Bs-916. These data suggested that the mutation of three key amino acids in ComA greatly affected the biological activity of Bacillus subtilis. ComA protein 3D structure prediction and motif search prediction indicated that ComA has two obvious motifs common to response regulator proteins, which are the N-terminal response regulator receiver motif and the C-terminal helix-turn-helix motif. The three residues in the ComA N-terminal portion may be involved in phosphorylation activation mechanism. These structural prediction results implicate that three mutated residues in the ComA protein may play an important role in the formation of a salt-bridge to the phosphoryl group keeping active conformation to subsequent regulation of the expression of downstream genes.