• Title/Summary/Keyword: P-V value

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소양호에서의 유기인산염 분해율

  • 최승익;안태석;김범철
    • Korean Journal of Microbiology
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    • v.30 no.2
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    • pp.113-118
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    • 1992
  • To elucidate a part of phosphorus cycle an'd the nutritional status of phosphorus. the degradation rates of organic phosphate by phosphatase activity (PA). were studied at the water column of Lake Soyang. from March 1900 to April 1991. Phosphatase activity showed the range of 1-2220 nMillhr. Its maximum value was recortled on August and minimum during October and November. The PA and chlorophyll u v~luess howed high correlation coefficent (0.69). and the values of specific astivity was highest during Winter, 45-12\ulcorner nMihripg chl. a, and lowest on October. 2 nMl hripg chl. 11. The values of PA and bioavailable dissolved inorganic phosphate (DIP) showed negative correlation in surface water. During destratification period (Oct. and Nov. 1990). the values of PA were about 11240 times lower than those during August albeit high concentration of chlorophyll a (1.7- 7.2 mglm'). Such results seem to be cause'd by DIP supply from the metalimnion. hypolimnion ant1 sediment. With these results. phosphate was sufficient to sustain the biomass in Autumn. and internal loading of phosphate shoultl accelerate the eutrophication in Lake Soyang.

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Purification, Characterization, and Partial Primary Sequence of a Major-Maltotriose-producing $\alpha$-Amylase, ScAmy43, from Sclerotinia sclerotiorum

  • Ben Abdelmalek-Khedher, Imen;Urdad, Maria Camino;Limam, Ferid;Schmitter, Jean Marie;Marzouki, M. Nejib;Bressollier, Philippe
    • Journal of Microbiology and Biotechnology
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    • v.18 no.9
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    • pp.1555-1563
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    • 2008
  • A novel $\alpha$-amylase ($\alpha$-1,4-$\alpha$-D-glucan glucanohydrolase, E.C. 3.2.1.1), ScAmy43, was found in the culture medium of the phytopathogenic fungus Sclerotinia sclerotiorum grown on oats flour. Purified to homogeneity, ScAmy43 appeared as a 43 kDa monomeric enzyme, as estimated by SDS-PAGE and Superdex 75 gel filtration. The MALDI peptide mass fingerprint of ScAmy43 tryptic digest as well as internal sequence analyses indicate that the enzyme has an original primary structure when compared with other fungal a-amylases. However, the sequence of the 12 N-terminal residues is homologous with those of Aspergillus awamori and Aspergillus kawachii amylases, suggesting that the new enzyme belongs to the same GH13 glycosyl hydrolase family. Assayed with soluble starch as substrate, this enzyme displayed optimal activity at pH 4 and $55^{\circ}C$ with an apparent $K_m$ value of 1.66 mg/ml and $V_{max}$ of 0.1${\mu}mol$glucose $min^{-1}$ $ml^{-1}$. ScAmy43 activity was strongly inhibited by $Cu^{2+}$, $Mn^{2+}$, and $Ba^{2+}$, moderately by $Fe^{2+}$, and was only weakly affected by $Ca^{2+}$ addition. However, since EDTA and EGTA did not inhibit ScAmy43 activity, this enzyme is probably not a metalloprotein. DTT and $\beta$-mercaptoethanol strongly increased the enzyme activity. Starting with soluble starch as substrate, the end products were mainly maltotriose, suggesting for this enzyme an endo action.

The Environmental Factors Affecting the Distribution and Activity of Bacteria in the Estuary of Naktong River (낙동강 하구의 세균분포와 활성에 미치는 환경요인)

  • 안태영;조기성;하영칠
    • Korean Journal of Microbiology
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    • v.29 no.5
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    • pp.329-338
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    • 1991
  • From July 1985 to December 1986, 28 variables of phycal-chemical factors, bacteria and heterotrophic activity were investigated 17 times at 3 stations in the estuary of Naktong River and the influences of environmental factors to bacterial population and heterotrophic activity were analyzed through multiple regression. The results of multiple regression were as follows. At station 1, total bacteria and heterotrophic bacteria(Z-25) could explain 57% of the variation of maximum uptake velocity for glucose and 54% of turnover time for glucose was explained by total coliform bacteria and MBOD, Sixty four percent of the variation of Kt+SN was accounted for salinity, MBOD-N and inorganic phosphate. Turnover rate for acetate was also accounted for the change of MBOD-P by 56%. At station 2 maximum uptake velocity for glucose depends on MBOD-N by 81%; turnover time on bacteria by 50%; Kt+Sn on avilable nutrient by 61%. More than 50% of maximum uptake velocity and turnover time for glucose were influenced by bacteria and that of Kt+Sn by the change of nutrient in the surface water of station 3. In the bottom water of station 3, the change of maximumuptake velocity, turnover time and Kt+Sn for glucose was controlled by total bacteria and available nutrient, bacteria, the change of nutrient salts respectively. On the whole, more than 50% of maximum uptake velocity and turnover time for glucose could be due to the change in the number of bacetria and the value of Kt+Sn was affected by the change of nutrient salts. Turnover rate for acetate was controlled by available phosphate at station 1 and by bacteria at station 2 and 3, which showed a distinct difference between the environmental factors which govern the rate of glucose and acetate uptake in the Naktong esturine ecosystem. And bacterial communities were controlled by available nutrients at station 1, by nutrient salts and salinity at station 2 and in the surface water of station 3 and by salinity in the bottom water of station 3.

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Rheological Properties of Doen-Jang(Korea Fermented Soybean Paste) Suspensions (된장 현탁액의 리올로지적 성질)

  • Lee, Chul-Weon;Hwang, Eung-Soo;Lee, Shin-Young;Pyun, Yu-Ryang
    • Korean Journal of Food Science and Technology
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    • v.22 no.2
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    • pp.111-115
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    • 1990
  • The rheological properties of soybean paste suspensions as affected by concentrations and heating were studied. All suspensions of soybean paste showed thioxotropic plastic behavior. The concentrated suspensions below the ratio of water to soybean paste, 1.25 exhibited Figure-eight phenomena which were presented by two Intersections of the up curve with the dorm curve of shear rate. The dilute suspension (soybean paste: water=1:2) showed H type hysteresis loop at low temperature. But increasing the heating temperature above $60^{\circ}C$, the hysteresis loop exhibited a Figure eight phenomenon. Consistency index of the suspensions increased remarkably by heating and showed the highest value around isoelectric point of the protein. It was suggested that the proteinous nitrogen might be the main component taking part in viscous behavior under different pH and heat treatment.

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Study on Electrical Properties of X-ray Sensor Based on CsI:Na-Selenium Film

  • Park Ji-Koon;Kang Sang-Sik;Lee Dong-Gil;Choi Jang-Yong;Kim Jae-Hyung;Nam Sang-Hee
    • Transactions on Electrical and Electronic Materials
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    • v.4 no.3
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    • pp.10-14
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    • 2003
  • In this paper, we have introduced the x-ray detector built with a CsI:Na scintillation layer deposited on amorphous selenium. To determine the thickness of the CsI:Na layer, we have estimated the transmission spectra and the absorption of continuous x-rays in diagnostic range by using computer simulation (MCNP 4C). A x-ray detector with 65 ${\mu}m$-CsI:Na/30 ${\mu}m$-Se layer has been fabricated by a thermal evaporation technique. SEM and PL measurements have been performed. The dark current and x-ray sensitivity of the fabricated detector has been compared with that of the conventional a-Se detector with 100 ${\mu}m$ thickness. Experimental results show that both detectors exhibit a similar dark current, which was of a low value below $400 pA/cm^2$ at 10 V/${\mu}m$. However, the CsI:Na-Se detector indicates high x-ray sensitivity, roughly 1.3 times that of a conventional a-Se detector. Furthermore, a CsI:Na-Se detector with an aluminium reflective layer shows a 1.8 times higher x-ray sensitivity than an a-Se detector. The hybrid type detector proposed in this work exhibits a low dark current and high x-ray sensitivity, and, in particular, excellent linearity to the x-ray exposure dose.

Drastic Brightness and Color Variations of the New Discovered Polar OTJ 071126+440405

  • Kim, Yong-Gi;Yoon, Joh-Na;Andronov, Ivan L.;Breus, Vitalii V.;Dubovsky, P.;Chinarova, Lidia L.;Han, Won-Yong
    • The Bulletin of The Korean Astronomical Society
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    • v.37 no.1
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    • pp.59-59
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    • 2012
  • Self-consistent mathematical model of the exotic object OTJ 071126+440405= CSS 081231:071126+440405 is discussed. The system was discovered as a polar at the New year night 31.12.2008/01.01.2009 by D.Denisenko (VSNET Circ), and we have initiated an international campaign of photometric and polarimetric observations of this object (totally -80 runs in Ukraine, Korea, Slovakia, Finland, USA) as a part of the "Inter-Longitude Astronomy" (ILA) project on monitoring of variable stars of different classes (Andronov et al., 2003). Here we present the geometrical and physical model of the system in the low luminosity state and in the intermediate luminosity state as well as in the high luminosity state. As the system is of -20 mag at minimum, no spectral observations were made to determine parameters of the red dwarf. From the statistical relationship, the mass of the red dwarf is estimated to be -0.165 solar masses, for the white dwarf (from eclipse duration) - from 0.5 to 1.76 solar masses. As the system resembles ER UMa in some characteristics, the lower value may be assumed. The inclination of the system and other physical parameters are estimated. The object is an excellent laboratory to study multiple physical processes in the magnetic systems.

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Formation and Characterization of Polyvinyl Series Organic Insulating Layers (폴리비닐 계열 유기절연막 형성과 특성평가)

  • Jang Ji-Geun;Jeong Jin-Cheol;Shin Se-Jin;Kim Hee-Won;Kang Eui-Jung;Ahn Jong-Myong;Seo Dong-Gyun;Lim Yong-Gyu;Kim Min-Young
    • Journal of the Semiconductor & Display Technology
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    • v.5 no.1 s.14
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    • pp.39-43
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    • 2006
  • The polyvinyl series organic films as gate insulators of thin film transistor(TFT) have been processed and characterized on the polyether sulphone (PES) substrates . The poly-4-vinyl phenol(PVP) and polyvinyl toluene (PVT) were used as solutes and propylene glycol monomethyl ether acetate(PGMEA) as a solvent in the formation of organic insulators. The cross-linking of organic insulators was also attempted by adding the thermosetting material, poly (melamine-co-formaldehyde) as a hardener in the compound. The electrical characteristics measured in the metal-insulator-metal (MIM) structures showed that insulating properties of PVP layers were generally superior to those of PVT layers. Among the layers of PVP series; copolymer PVP(10 wt%), 5wt% cross-linked PVP(10 wt%), copolymer PVP(20 wt%), 5 wt% cross-linked PVP(20 wt%) and 10 wt% cross-linked PVP(20 wt%), the 10 wt% cross-linked PVP(20 wt%) layer showed the lowest leakage current of 1.2 pA at ${\pm}10V$. The ms value of surface roughness and the capcitance per unit area are 2.41 and $1.76nF/cm^2$ in the case of 10 wt% cross-linked PVP(20 wt%) layer, respectively.

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The Development of Estimation Model for Iron Compound Originated from Anaerobic Microbial Corrosion (혐기성 미생물부식에 의해 생성되는 철화합물 추정식 개발)

  • Jeon, Seok-Jun
    • Journal of Korean Society on Water Environment
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    • v.18 no.4
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    • pp.379-386
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    • 2002
  • In this study, estimation model for iron compound originated from upflow, anaerobic fixed bed reactor, which treats sewage domestic wastewater, was developed. The estimation model was formulated by a mathematical expression which was based on the mass balance. Below the HRT of 60 minute, sulfide concentration combining with iron $FeS_2$ is the highest because the maximum sulfate consumption rate $V_{maxS}$ and half-saturation constant of sulfate $K_{mS}$ exert an important effect on the estimation model as temperature was increased. But increment of $FeS_2$ concentration is weakened above the HRT of 60 minutes and represent the lowest value at the HRT of 108 minutes. It implies that liquid phase distribution ratio of sulfide ${\alpha}r$ becomes lower as temperature was increased. While phosphorus concentration combining with iron $Fe_3(PO_4)_3$ is increased as HRT and temperature are increased, which is affected by phosphorus removal rate constant $k_p$. As the result of estimating the iron concentrations of corrosion by the model, the concentration of iron corrosion is higher than any other at the HRT of 108 minute and $20^{\circ}C$. The predicted values were compared with measured ones at different HRT(13.5, 27, 54, 108 min) and temperature(20, 25, $30^{\circ}C$). The experimental data could be fitted with the simulated curves. Therefore, the mathematical expression could be applicable to design full-scale wastewater treatment plants.

Study on Explosion Behavior of Air-born Rice Bran Dusts according to Ignition Energy (점화에너지 변화에 따른 쌀겨분진의 폭발 거동에 관한 연구)

  • 김정환;김현우;현성호;백동현
    • Fire Science and Engineering
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    • v.13 no.2
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    • pp.26-32
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    • 1999
  • We had investigated combustion pro야$\pi$ies of rice bran dusts. Decomposition of rice bran d dusts with temperature were investigated using DSC and the weight loss according to t temperature using TGA in order to find the thermal hazard of rice bran dusts, and the p properties of dust explosion in variation of their dust with the same particle size. Using H Hartman's dust explosion apparatus which estimate dust explosion by electric ignition after m making dust disperse by compressed air, dust explosion experiments have been conducted by v varying concen$\sigma$ation and size of rice br뻐 dust. According to the results for thermodynamic stability of rice bran dust, there are little change of initiation temperature of heat generation 때d heating value for used particle size. But i initiation temperature of heat generation decreased with high heating rate whereas d decomposition heat increased with particle size. Also, the explosion pressure was increased as t the ignition energy increased and average maximum explosion pressure was 13.5 kgv'cnt for 5 BJ/60 mesh and 1.5 뼈Ie미 dust concentration.

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Production, Nutritional Quality and In vitro Methane Production from Andropogon gayanus Grass Harvested at Different Maturities and Preserved as Hay or Silage

  • Ribeiro, G.O. Jr.;Teixeira, A.M.;Velasco, F.O.;Faria, W.G. Junior;Pereira, L.G.R.;Chaves, A.V.;Goncalves, L.C.;McAllister, T.A.
    • Asian-Australasian Journal of Animal Sciences
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    • v.27 no.3
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    • pp.330-341
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    • 2014
  • Andropogon gayanus is an important grass due to its high biomass production, drought tolerance and favorable growth on low fertility acidic soils. Currently, there is little research on the impact of growth stage on the nutritional quality or the degree of $CH_4$ production that may arise from this forage during ruminal fermentation. The objectives of this study were to determine the effects of regrowth stage of A. gayanus on its chemical composition, in vitro production of gas and CH4, as well as in vitro dry matter (DM) digestibility when grown under tropical Brazilian conditions and conserved as hay or as silage. The nutritional value of A. gayanus grass declined with increasing maturity; however digestible DM yield linearly increased. After 112 d of regrowth, A. gayanus produced higher quality silage (higher lactate and lower pH and butyrate content) and higher DM yield. However, the low levels of crude protein at this time would make protein supplementation a necessity for proper rumen fermentation. No differences in $CH_4$ kinetic parameters were found with advancing maturity or preservation method (hay or silage).