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Effect of Vitamin E and Selenium Administration on The Reproductive Performance in Dairy Cows

  • Kim, H.S.;Lee, J.M.;Park, S.B.;Jeong, S.G.;Jung, J.K.;Im, K.S.
    • Asian-Australasian Journal of Animal Sciences
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    • v.10 no.3
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    • pp.308-312
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    • 1997
  • Incidence of retained placenta in dairy cows was investigated in 120 parturitions. Prior to calving, cows were allotted into four groups; 1) control, 2) the infection of Vitamin E (500 IU), 3) the injection of selenium (40 mg), and 4) the infection of Vitamin E (500 IU) and selenium (40 mg). Selenium (Se) and Vitamin E were injected intramusculary 20 d prior to the estimated calving date. No effects of Se or Vitamin E administration alone was observed for number of service per conception, conception rate, and estrus rate (p > 0.05). But the Vitamin E administration with Se significantly (p < 0.05) reduced the incidence of retained placenta and the days required for calving the first service. Incidence of retained placenta was not significantly (p > 0.05) influenced by parity, but it was significantly (p < 0.01) influenced by season, especially, increased during July and August. Concentration of Se in plasma was not significantly (p > 0.05) changed during peripartum period whether the treatment. The results of this study on retained placenta suggest that this disorder will be reduced by the administration of Se and Vitamin E prior to calving in dairy cows.

고온 환경조건의 육용종계에서 비타민 섭취에 의한 난각질 저하 방지 효과

  • 지규만;정만기
    • Proceedings of the Korea Society of Poultry Science Conference
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    • 2003.05a
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    • pp.49-59
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    • 2003
  • Heat stress환경의 육용종계에서 사료에 첨가한 비타민 C(200 mg/kg)와 비타민 E(250 mg/kg)가 난각 품질 및 경골 강도에 미치는 영향을 확인하고자 실험을 실시하였다. 강제 환우에서 회복된 83주령의 Ross품종 육용종계 160수를 4처리 4반복 10수씩 개별 케이지에 수용 한 뒤, 10일간의 적응 기간을 둔 뒤, 3주간에 걸쳐 32$^{\circ}C$에서 지속적으로 온도를 유지하면서 사료 섭취량, 산란율, 폐사율, 난중, Haugh unit, 난각의 품질, 경골 파괴강도 그리고 혈액 중의 혈구 세포 등을 조사하였다. Heat stress는 폐사율, 산란율, 난중, 그리고 Haugh unit를 감소시키는 경향이 있었으나 통계적으로 유의하지는 않았다. 난각의 SWUSA와 압축 파괴강도는 비타민 C/E 첨가구에서 2, 3주차에 무첨가구보다 유의하게 높았으나 (P<0.05), 비타 C 또는 E 첨가구 사이에서는 유의차가 없었다. Heterophil과 lymphocyte의 숫자는 heat stress 동안에 각각 증가 또는 감소하였다. 처리구간에는 비타민 C/E 첨가구가 H/L/ratio 수준이 가장 낮았으며, 무첨가구가 가장 높은 경향을 나타내었다. 경골의 파괴강도는 비타민 C 첨가구에서 가장 높았다(P<0.05). 혈액 중의 비타민 C 농도는 비타민 C첨가구와 비타민 C/E 첨가구에서 각각 12.73 g/ml과 8.23 g/ml으로 높았다(P<0.05). 비타민 E 첨가구와 비타민 C/E 첨가구에서 혈액 중 비타민 E 농도가 유의하게 높았다 (P<0.05). Corticosterone 농도는 무첨가구에서 5.97g/ml으로 통계적으로 유의하게 높았다.(P<0.05).

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Water and energy partitioning of catchments under various climatic conditions (다양한 기후조건에 따른 유역에서의 물과 에너지 분배)

  • Sanghyun Yoo;Kyungrock Paik
    • Proceedings of the Korea Water Resources Association Conference
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    • 2023.05a
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    • pp.103-103
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    • 2023
  • 질량 및 에너지 보존 법칙은 수문현상을 포함한 자연 현상의 기본 법칙이다. 유역에서 물의 질량 보존은 강수량 P가 유출량 Q, 증발산량 E, 그리고 육역저수량변화 ΔS로 분할되는 것(P=Q+E+ΔS)을 의미하며 열 에너지 보존은 순복사에너지 Rn이 잠열 λE, 현열 H, 그리고 지열 G로 분할되는 것(Rn=λE+H+G)을 의미한다. 유역에서 물과 에너지의 분배 과정은 E로 연결되어 있으며 이 두 과정을 포괄적으로 이해하는 것은 기후 및 지표환경의 변화를 예측하고 대비하는데 중요하다. 이 연구에서는 미국 전역의 400여개 유역에 대한 정보를 제공하는 Model Parameter Estimation Project(MOPEX) 데이터를 이용하여 유역의 기후 조건에 따라 물과 에너지의 분배가 어떻게 달라지는지 Budyko 평면에서 분석했다. 장기간에 대해 ΔS와 G는 무시할 수 있다는 가정하에 건조한 유역일수록 P, Q, 그리고 E 모두 작게 나타나는데 P와 Q의 감소폭이 훨씬 크기 때문에 E의 P에 대한 비는 크게 나타났다. 또한 건조한 유역일수록 E는 작고 Rn이 크기 때문에 H가 크게 나타났으며 H가 큰 유역일 수록 유역의 최대 기온과 최저 기온의 차이가 크게 나타났다. 이러한 변화는 동일한 유역내에서 물과 에너지 분배의 시간적 변화로도 나타나고 있다.

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Temperature-Dependent Expression of Escherichia coli Thioredoxin Gene

  • Lee, Jin-Joo;Park, Eun-Hee;Ahn, Ki-Sup;Lim, Chang-Jin
    • BMB Reports
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    • v.33 no.2
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    • pp.166-171
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    • 2000
  • Thioredoxin is a multifunctional protein that is ubiquitous in microorganisms, animals and plants. Previously, the expression of the Escherichia coli thioredoxin gene (trxA) was found to be negatively regulated by cAMP. In the present study, the effect of temperature on the expression of the E. coli trxA gene was investigated. In order to examine the temperature effect, the fusion plasmid pCL70 that harbors the E. coli trxA P1P2 promoter was used. The other two fusion plasmids, pJH3 and pMH521 that were constructed in different vectors which harbor the E. coli trxA P2 promoter, were also used. When the E. coli strain MC1061/pCL70 was grown in a rich medium at $25^{\circ}C$, $34^{\circ}C$ and $42^{\circ}C$, the cells grown at $42^{\circ}C$ gave the highest $\beta$-galactosidase activity. The E. coli MC1061/pJH3 and MC1061/pMG521 cells showed increased $\beta$-galactosidase activity after the shift of the culture temperature to $42^{\circ}C$. The wild-type trxA gene of the E. coli MC1061 cells produced much higher thioredoxin activity at the higher temperature. These results support the conclusion that the E. coli trxA gene is regulated in a temperature-dependent manner. Especially the expression from its P2 promoter appeared to be sensitive to temperature.

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High-Level Expression of A Bacillus subtilis Mannanase Gene in Escherichia coli. (대장균에서 Bacillus subtilis의 Mannanase 유전자 과잉발현)

  • 권민아;손지영;윤기홍
    • Microbiology and Biotechnology Letters
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    • v.32 no.3
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    • pp.212-217
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    • 2004
  • The gene coding for mannanase from Bacillus subtilis WL-7, a number of glycosyl hydrolase family 26, was hyperexpressed in Escherichia coli. Two recombinant plasmids, pE7MAN and pENS7, were constructed by introducing the complete mannanase gene and the mature mannanase gene lacking N-terminal signal peptide region into a expression vector pET24a(+), respectively. The level of mannanase produced by E. coli BL21 (DE3) carrying pENS7, which included the mature mannanase gene, was considerably higher than that by E. coli BL21 (DE3)/pE7MAN. Almost mannanase produced by the recombinant E. coli carrying pENS7 at growth temperature of $37^{\circ}C$ existed as inactive enzyme of insoluble form. Growth at temperature below $31^{\circ}C$ increased the soluble fraction of mannanase having catalytic activity in the recombinant E. coli cells. The highest productivity of active mannanase was observed in cell-free extract of the recombinant E. coli grown at growth temperature ranging from $25^{\circ}C$ to $28^{\circ}C$, while mannanase activity per soluble protein of the cell-free extract was highest in the cells grown at $^31{\circ}C$.

Construction of shuttle vectors expressing the cry11Aa gene and their mosquitocidal activity (모기살충성 cry11Aa 유전자를 발현하는 벡터의 구축과 모기살충효과)

  • Lee, Dae-Weon;Kim, Ho-San;Je, Yeon-Ho;Kim, Joo-Up;Yu, Hyo-Sok;Kang, Seok-Kwon
    • The Korean Journal of Pesticide Science
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    • v.2 no.1
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    • pp.91-96
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    • 1998
  • For the expression of the cry11Aa gene highly toxic to dipteran insects, we constructed two cyanobacteria-Escherichia coli(E. coli) shuttle vectors, pCYASK5-l and pCYASK5-2. These vectors were transformed into E. coli and selected with kanamycin. The expression of the cry11Aa gene in E. coli was characterized by SDS-polyacrylamide gel electrophoresis and Western blot analysis. Two E. coli transformants harboring pCYASK5-1 and pCYASK5-2 expressed the cry11Aa gene in size of 72 kDa and 64 kDa, respectively and showed over 89% mortality against Culex pipiens larvae.

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Association of a p53 Codon 72 Gene Polymorphism with Environmental Factors and Risk of Lung Cancer: a Case Control Study in Mizoram and Manipur, a High Incidence Region in North East India

  • Saikia, Bhaskar Jyoti;Das, Mandakini;Sharma, Santanu Kumar;Sekhon, Gaganpreet Singh;Zomawia, Eric;Singh, Yanglem Mohen;Mahanta, Jagadish;Phukan, Rup Kumar
    • Asian Pacific Journal of Cancer Prevention
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    • v.15 no.24
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    • pp.10653-10658
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    • 2015
  • Background: A very high incidence of lung cancer is observed in Mizoram and Manipur, North East India. We conducted a population based case control study to establish associations of p53 codon 72 polymorphisms and interactions with environmental factors for this high incidence. Material and Methods: A total of 272 lung cancer cases and 544 controls matched for age (${\pm}5years$), sex and ethnicity were collected and p53 codon 72 polymorphism genotypes were analyzed using a polymerase chain based restriction fragment length polymorphism assay. We used conditional multiple logistic regression analysis to calculate adjusted odds ratios and 95% confidence intervals after adjusting for confounding factors. Results: p53 Pro/Pro genotype was significantly associated with increased risk of lung cancer in the study population (adjusted OR=2.14, CI=1.35-3.38, p=0.001). Interactions of the p53 Pro/Pro genotype with exposure to wood smoke (adjusted OR=3.60, CI=1.85-6.98, p<0.001) and cooking oil fumes (adjusted OR=3.27, CI=1.55-6.87, p=0.002), betel quid chewing (adjusted OR=3.85, CI=1.96-7.55, p<0.001), tobacco smoking (adjusted OR=4.42, CI=2.27-8.63, p<0.001) and alcohol consumption (adjusted OR=3.31, CI=1.10-10.03, p=0.034) were significant regarding the increased risk of lung cancer in the study population. Conclusions: The present study provided preliminary evidence that a p53 codon 72 polymorphism may effect lung cancer risk in the study population, interacting synergistically with environmental factors.

The Relation of the Elevated Plasma Lipid Levels to Plasma Vitamin E Status and Activities of Erythrocyte Glutathione Perosicase in Smokers (흡연인들에서 증가된 혈장지질 농도가 비타민 E 영양상태와 글루타티온 과산화효 활성에 미치는 영향)

  • 윤군애
    • Journal of Nutrition and Health
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    • v.31 no.8
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    • pp.1254-1262
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    • 1998
  • This study has done to investigate the relationship between the icreased lipid oncentration caused by smoking and plama levels of vitamin A and vitamin E, antiodative enzyme activity, and lipid peroxidation , in 52 male smokers and 32 non-smokers, Dietary vitamin A and vitamin E intake was imilar in both smokers and non-smokers. Absolute plasma concentrations of vitamin A and vitamin E were not significantly different between two groups, whereas vitamin E/cholesterol ration in plasma was low or in smokers than in that of non-smokers(p<0.05). It was considered that this lowered effect was due to the elevated plasma lipid concentration rather than oxidant stress derived from smoking, in view of the fact that smokers had higher cholesterol (15.2%) adn LDL-C(26.6%) levels than non-smokers. In non-smokers, plasma thiobarbiturin acid reactive substances(TBARS) conrrelated positively with total cholesterol(r=0.63466, p<0.001), LDL-C level(r=0.57166, p<0.01) , and LDL-C/HDL-C ratio(r=0.45926, p<0.05) . Activities of glutathione perosidase(GSH-Px) , superoside dismutase(SOD), and catalse made no difference in both groups. However, it was observed in non-smokers that GSH-Px activity had negative correlations with total cholesterol(r=-0.67293, p<0.001), LDL-C level(r=-0.62878, p<0.001), and LDL-C/HDL-C ratio (r=-0.58824, p<0.01), indicating that there was a dependent relationship between lipid perosidation and plasma lipid level. The smokers also showed negative correlations for GSH-Px activity with total cholesterol (r=-0.29946, p<0.05) and LDL-c level (r=0.45914, p<0.001), and LDL-C/HDL-c ratio(r=-0.35438, p<0.05). It seemed that the lipid that the lipid level elevated by sustaines smoking resulted in reducing vitamin E/cholesterol ratio and proportion of antioxidant to oxidant load, and then GSH-Px activity, with insufficient removal of free radicals(TBARS 2.43$\pm$0.51 and 1.81$\pm$0.15nmol/ml in smokers and non-smokers, respectively). These findings suggest that higher plasma lipid levels may play a more important role in perturbing the antioxidant defense system including vitamin E status and GSH-Px activity, at least in circumstances that increase lipid concentration . In addition, in exposure to free radicals like those in cigarette smoke. In those cases the ratio of vitamin E/lipid in plasma can be a more indicator of vitamin E status than plasma levels of vitamin E alone.

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A Retrospective Study on Canine Epilepsy: Etiological Distribution, Therapeutic Outcome, and Survival Time

  • Park, Seo-Yeon;Jeong, Yoonsoo;Yun, Taesik;Jung, Dong-In;Chang, Dong-Woo;Kang, Ji-Houn;Yang, Mhan-Pyo;Kang, Byeong-Teck
    • Journal of Veterinary Clinics
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    • v.36 no.3
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    • pp.150-154
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    • 2019
  • The purpose of this study was to investigate the etiological distribution, therapeutic outcome, and survival time in canine epilepsy. The medical records of 57 epileptic dogs were reviewed for the evaluation of etiological distribution. Among them, 27 dogs (47%) and 30 dogs (52%) had idiopathic epilepsy (IdE) and structural epilepsy (StE), respectively. Twenty-nine dogs (IdE: 16 dogs, StE: 13 dogs) were evaluated for therapeutic outcome and survival time. The incidence of generalized epileptic seizure (IdE, 56% vs. StE, 44%; P = 0.043) and the median seizure frequency at the time of first presentation (IdE, 2.0/month vs. StE, 13.3/month; P < 0.01) were significantly different between the two groups. Although pre-treatment seizure frequency and duration were not different, the median duration of seizure in the IdE group (0.5 min) was significantly shorter than that in the StE group (3 min) after treatment (P < 0.01). In addition, the median frequency of seizure was relatively lower in the IdE group (0.25/month) compared to the StE group (2.00/month) following antiepileptic therapy (P = 0.053). The median survival time of the IdE group (1.5 years [95% CI, 1.0-2.3 years]) was significantly longer than that of the StE group (0.4 year [95% CI, 0.2-1.3 years]) (P < 0.01). The information on etiological data and intracranial lesions may be useful for predicting treatment response and prognosis in epileptic dogs.

Molecular cloning and foreign gene expression of restriction endonuclease fragments of the Hc nuclear polyhedrosis virus DNA (Hc nuclear polyhedrosis virus DNA 제한효소절편의 molecular cloning 과 외래 유전자 발현)

  • Lee, Keun-Kwang
    • Journal of fish pathology
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    • v.8 no.1
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    • pp.31-36
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    • 1995
  • Hc nuclear polyhedrosis virus DNA genome was digested with EcoRI endonuclease, these partial fragments were recombined into the pUC8 plasmid vector and transformed in E. coli JM 83 cell. The genome DNA has 24 EcoRI fragments and 12 fragments of them were subcloned. The four recombinants were named as eNP-O, eNP-Q, eNP-R and eNP-S. The expression of foregin gene of the recombinants was investigated by analysing protein patterns on the SDS-PAGE. The eNP-O, eNP-Q and eNP-R were expressed a different weight of protein as comparision with potypeptide bands of E. coli JM 83 host cell.

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