• Journal of Environmental Science International
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• v.2 no.3
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• pp.217-225
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• 1993

This study was performed at Sa-Sang Industrial area in Pusan City. To measure the total suspended particulates(T.S.P) and $SO_3$ following places was selected industrial area, down town, residentail area. T.S.P was collected on glass filters by High-Volume Air Sampler, $SO_3$ was measured by PbO2 Candle method. The collected T.S.P was analyzed for various anlon(for example, $NO_3$, $SO_4$, $PO_4$, F, Cl) by ion Chromatography, and Pb by Inductively Coupled Plasma.

• Journal of Life Science
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• v.20 no.8
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• pp.1201-1206
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• 2010

We performed a comparative analysis using a Real-time PCR (Polymerase Chain Reaction) and LC/MS (Liquid-Chromatograph/Mass Spectrometer) method in order to detect microcystin in environmental sources. Among the three different primer sets tested for microcystin using three positive strains of Microcystis aeruginosa by Real-time PCR assay, only TOX2P/TOX2M primer pairs were able to detect Microcystis aeruginosa. According to the results of a survey carried out from June 2009 to September 2009, 11 out of 11 (100%) raw water samples were were found to have microcystin when the Real-Time PCR and LC/MS method was used, with total microcystin concentration ranging from 5.98~219.0 ${\mu}g/l$. A microcystin removal treatment process was used to ensure entire removal, by passing it through a BAC filtration step. It was concluded that real-time PCR assay can be used to estimate micrucystin detection more rapidly and easily than the LC/MS method.

• Journal of Environmental Science International
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• v.16 no.1
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• pp.81-94
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• 2007

The purpose of this work is to investigate the water quality change characteristics of treated water in water distribution systems of Water Treatment Plants (WTPs) of Jeju City. For this, the raw water, treated water and tap water that did not pass (named as not pass-tap water) and passed through the water storage tank (named as pass-tap water) were sampled and analyzed monthly from September 2001 to August 2002, for four (W, S, B and O) WTPs except for D WTP (where treated water is not supplied continuously) among WTPs of Jeju City. The concentrations of $NO_3^-$ and $Cl^-$ of treated water in distribution systems changed little, but changed seasonally, which is considered to be based on the seasonal variation of the quality of raw water. The pH of treated water changed little in distribution systems for S WTP, but for the other WTPs, the pH of not pass-tap water was similar to that of treated water and the pH of pass-tap water was higher than that of treated water. The turbidity of treated water in distribution systems changed little except for W2 of W WTP and S4 and S5 of S WTP, where it was higher than that of each treated water. The residual chlorine concentrations between treated water and not pass-tap water changed little, but those between treated water and pass-tap water changed greatly, based on the its long residence time in water storage tank and so its reaction with organic matter, etc or its evaporation. The concentrations of TTHMs (total trihalomethanes) and $CHCl_3$ that induce cancers in water distribution systems of these WTPs, were much lower than their water quality criteria and those in other cities. The concentrations of TTHMs of treated water and not pass-tap water were similar, but concentrations of pass-tap water were 1.5 to 2.0 times higher than those of treated water and not pass-tap water, due to the reaction of residual chlorine and organic matter, etc, with the result of long residence time in water storage tank.

• Asian Pacific Journal of Cancer Prevention
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• v.13 no.5
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• pp.2363-2367
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• 2012

Objective: To investigate the association between the connexin 37 C1019T polymorphism and Helicobacter pylori infection in patients with gastric cancer. Methods: 388 patients with gastric cancer (GC), 204 with chronic superficial gastritis (CSG) were studied. H. pylori was detected by gastric mucosal biopsies biopsy dyeing method. Connexin 37 gene polymorphism 1019 site genotypes were determined by gene sequencing technology. Genotypes and alleles frequencies were compared. Results: (1) Connexin37 gene 1019 site distribution frequency (CC type, TC type, TT type) in the CSG group was 18.1%, 45.1% and 36.8%; in the stomach cancer group it was 35.1%, 45.9% and 19.%, conforming to the Hardy-Weinberg euilibrium. (2) In comparison with CSG group, the frequency of Connexin37 C allele was higher in the gastric cancer group (58.0% vs 40.7%, OR = 2.01, 95%CI = 1.58-2.57, P < 0.01). The prevalence of gastric cancer risk was significantly increased in the carriers of C allele (CC+TC) than in TT homozygote (OR = 2.47, 5%CI = 1.68- 3.610. (3) Gastric cancer patients complicated with Hp infection 211 cases, gastric cancer group of the male patients with HP positive patients with 187 cases, 40 cases of female patients with negative patients, 24 cases were HP positive, negative in 137 cases, control group male patients, 28 cases were Hp positive, negative in 95 patients, female patients with Hp positive 6 cases, 75 cases were negative. On hierarchical analysis, the male group OR value was 15.9 (95%CI to 9.22-27.3), and the female OR was 2.19 (95%CI 0.88-5.59), indicating a greater contribution in males (P <0.01). After elimination of gender effects, positive HP and gastric cancer were closely related (OR 8.82, 95% CI: 5.45-14.3). (4) The distribution frequency of C allele in patients with Hp infection was much higher than that in Hp negative cases in the GC group (64.5% vs 47.0%, OR = 2.05, 95%CI = 1.54-2.74, P < 0.01). Compared with TT homozygotes, (CC+TC) genotype prevalence of gastric cancer risk increased significantly (OR = 2.96, 5%CI = 1.76-2.99 ). Conclusion: The T allele in the connexin37 gene might not only be associated with gastric cancer but also with H. pylori infection.

• Journal of Korean Society of Environmental Engineers
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• v.35 no.12
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• pp.943-952
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• 2013

Algae blooms have soared recently in the lakes across the nation due to eutrophication. Blue-green algae cause unpleasant scene, produce taste and odor problem, and hinder processes in drinking water treatment. Algae toxicity monitoring has been strengthened, because the damages of wild lives and livestocks by algal toxins have been reported. Investigation on the characteristics of cyanobacterial occurrence and concentration distribution of Cyanotoxins in Hoeya reservoir have been conducted. Physical and chemical influences of water environment on cyanobacterial occurrences have also been studied. Movements of four species of Microcystin and five species of Anatoxin-a among Cyanotoxins were observed by LC-MS/MS analysis. Microcystis spp. among the cyanobacteria have mainly dominated in the Hoeya reservoir during the investigating period. The density of cyanobacteria were positively correlated with temperature and pH of water. Highest concentrations of Microcystin-LR and Microcystin-RR were $0.424{\mu}g/L$ and $0.117{\mu}g/L$ at the sampling points. Total concentration of Cyanotoxins in water coming into the water treatment plant was $0.182{\mu}g/L$, and they were not detected in treated water.

• Journal of Food Hygiene and Safety
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• v.35 no.3
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• pp.225-233
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• 2020

By the standards and specifications for hygiene products, three test methods for formaldehyde are specified for each item type of hygiene product. After derivatization using acetylacetone and 2,4-dinitrophenylhydrazine (2,4-DNPH), formaldehyde is analyzed by spectrophotometer and high-performance liquid chromatography (HPLC). Validation of the three test methods was performed on tissue, diaper lining and waterproof layer, and panty liner products. The results of linearity (R²), limit of detection (LOD), limit of quantification (LOQ), recovery rate (%) and reproducibility (%), showed that all three methods are suitable for analyzing formaldehyde in hygiene products. After derivatization with 2,4-DNPH and cetylacetone, formaldehyde was analyzed at 0, 3, 6, 9, 24 and 48 hours by HPLC. Formaldehyde derivatized with 2,4-DNPH showed no statistically significant change in formaldehyde peak area over time (P>0.05). But, acetylacetone-derivatizated formaldehyde showed a negative correlation coefficient (r) over time (P<0.01). We investigated the residual amounts of formaldehyde in 205 hygiene products distributed in Busan. Among 74 disposable diaper products tested, 73 had low concentrations of formaldehyde (0.13-29.87 mg/kg). Moreover, formaldehyde was not detected in any of 78 tissue, 27 disposable paper towel, 12 disposable dishcloth, 7 paper cup, one brand of paper straw and 6 disposable napkin products.

• Journal of Environmental Science International
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• v.3 no.2
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• pp.101-109
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• 1994

Chlorinated water in swimming pools contains chloroform at elevated levels compared to chlorinated drinking water Chloroform levels in four indoor swimming pools(swimming pools A, B and C in a city of Korea and swimming pool D in a city of New Jersey in the United States) were examined. The chloroform levels in the water of swimming pool C (city-managed) weve shown to be significantly(p=0.0001) different from those of private swimming pools A and B: the mean chloroform levels in the pools A, B, and C are 22.8, 17.8, and 31.1 $\mu\textrm{g}$/l, respectively. Furthermore, all of these chloroform levels are significantly(P=0.0001) different from those of New Jersey: chloroform concentration of the Korean pools ranged from 10.9 $\mu\textrm{g}$.l to 47.9 $\mu\textrm{g}$/l with a mean of 23.2 $\mu\textrm{g}$/l, while it ranged from 27 $\mu\textrm{g}$/l to 96$\mu\textrm{g}$/l with a mean of 64.4 $\mu\textrm{g}$/l in the New Jersey pool. The disinfection processes would cause part of this difference since the swimming pools in Korea applied both chlorination and ozonation method, while the swimming pool in New Jersey used chlorination method only. It was implied that swimming parameters inconsistently vary, resulting in fluctuation of and no constant accumulation of chloroform in the water with the change of time for the day. A regression analysis showed no relationships between sampling time and chloroform concentrations for the sampling day in the swimming pools of Korea. A F-test indicated no significant difference of chloroform concentrations in the morning and afternoon samples collected in the swimming pools. Ingestion dose was estimated to be 0.58$\mu\textrm{g}$ from an hour swimming in a city of Korea, taking into accounting an average of 23.2 $\mu\textrm{g}$/l in swimming pools in the city In extreme situation, the ingestion dose was estimated to be 12.0 $\mu\textrm{g}$ from an hour swimming in a city of Korea.

• Asian Pacific Journal of Cancer Prevention
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• v.16 no.7
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• pp.2813-2818
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• 2015

Objective: To investigate the regulatory effect of curcumin on expression of signal transducer and activator of transcription 3 (STAT3) in skin squamous cell carcinoma tissues as well as possible mechanisms of curcumin in prevention and treatment of skin squamous cell carcinoma. Materials and Methods: Highly invasive A431 cells were treated with curcumin at various doses .The cytotoxic effects of treatment with 5, 10, 15, 20, 25, 30, 35, 40 and 50 umol/L curcumin for 24, 48 and 72 hours on A431 cells were measured by MTT assay. The invasion capacity of cells treated with 5, 10 and 15 umol/L curcumin was measured by Transwell test, while adhesive ability was assessed by cell adhesion assay. The effects of 5,10 and 15 umol/L curcumin on expression levels of STAT3 were determined by Western blotting and on transcription levels of STAT3 mRNA by RT-PCR. Results: Treatment with curcumin at a doses of more than 15 umol/L for more than 24 hour inhibited the growth of A431 cells in a time-and dose-dependent fashion (p<0.001). The doses of 15 umol/L and less for 24 hours showed no significant cytotoxic effects on the cells, survival rates being more than 85%.The invasion and adhesive abilities decreased gradually with the increasing curcumin concentration, 15 umol/L exerting the strongest inhibitory effects (p<0.05). Curcumin showed significant dose-dependent inhibitory effects on the transcription level of STAT3 mRNA (p<0.05). Conclusions: Curcumin may reduce the invasive ability of A431 cells by inhibiting the activation of STAT3 signal pathway and expression of STAT3 as a target gene in the pathway.

• Korean Journal of Agricultural Science
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• v.32 no.1
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• pp.1-8
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• 2005

This study was carried out to examine the effect of heavy traffic on chemical properties of soil in roadside trees of Daejeon city. Soil samples at 0~10cm and 10~30cm depths were collected from soil of the roadside trees, Platanus occidentalis, and Ginko biloba, Soil pH in heavy traffic regions were around 7.0 at 0~10cm and 10~30cm soil depths because of spraying of calcium chloride for snow moving. The concentrations of Fe, Cd, Cu, Zn, and Pb in soil were higher in heavy traffic regions(Daejeon Station and Daehwa Industrial Complex) than in light traffic region (Chungnam National University). The result could be from rubbing and wear of car tire and metals when they travel.

• Korean Journal of Occupational Health Nursing
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• v.23 no.4
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• pp.197-207
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• 2014

Purpose: The purpose of this study was to investigate the status and characteristics of visiting nurses' management for elders with cognitive impairment living in a community focused on health centers in a metropolitan city and five medium-sized cities. Methods: Data were collected from 47 visiting nurses working in a metropolitan city and 47 visiting nurses working in five medium-sized cities from November to December 2012. Results: There were no statistically significant differences in knowledge, attitudes, and nursing behaviors of cognitive impairment between two groups (F=2.13, p=.148; F=3.64, p=.060; F=0.28, p=.595). Among the elders referred to a physician in a metropolitan city by visiting nurses, 42.4% were diagnosed as mild cognitive impairment and 15.2% were diagnosed as severe dementia. The major intervention programs which visiting nurses currently applied for elders were medication and exercise intervention programs, and the intervention programs which they would want to apply in the future were playing, music and recall intervention programs. Conclusion: The cognitive impairment screening test can be done effectively by visiting nurses. This study also suggests to develop various kinds of intervention programs to improve cognitive function for elders living in a community.